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The map label for this gene is hemK [H]

Identifier: 78065072

GI number: 78065072

Start: 468686

End: 469528

Strand: Reverse

Name: hemK [H]

Synonym: Bcep18194_A3596

Alternate gene names: 78065072

Gene position: 469528-468686 (Counterclockwise)

Preceding gene: 78065073

Following gene: 78065071

Centisome position: 12.71

GC content: 72.12

Gene sequence:

>843_bases
ATGCCCGACACCACCGCCGACGAACTGCTGCGCGCGTCGCCGCTCGACGCGGTCGATGCGCGCGTGTTGCTCGCGCACGC
GCTCGGCTGGACCCGCACGCAACTGATCACGCGCGGCGACGCGACGCTCGACGCAGCCGCGGTCGAACGCTACCGCGCGC
TCGAGGCACGCCGCCTGGCCGGCGAGCCCGTCGCCCAGCTCGTCGGGATGCGCGAATTCTTCGGCCGGCCATTCGACGTG
ACGCCCGACGTGCTGATCCCGCGCCCGGAAACCGAACTGCTCGTCGAAGCCGCACTCGATGCGATCGACGGGCTGCCGCA
TCCGGCCGTGCTCGATCTCGGCACGGGCAGCGGCGCGATCGCCGTATCGATCGCCGCCGAGCGTCCCGATGCGCGCGTGT
GGGCGCTCGACCGTTCGCCGGCCGCACTCGCGGTCGCGCAACGCAACGCCGGCAAGCTGCTCGACGCGCGCCGCCCCGGC
GGCCCGCTGCACTGGCTGGAGAGCGACTGGTATGCGGCGCTCGATCCGGCGCTCGCATTCGACACGATCGTCAGCAATCC
GCCGTACATCGCGCAGCACGATCCGCACCTCGCGCAAGGCGACCTGCGTTTCGAGCCGCGCGGCGCACTCACCGACGATG
CCGACGGCCTCAGCGCGATCCGCACGATCGTCGCGGGCGCCGGCACTTATCTGAAACCGGGCGGCACGCTCTGGATCGAG
CATGGCTACGACCAGGCCGAGGCCGTGCGCGCAGTGCTCGCATCGCACGGCTTCGTCGCGGTCGAATCGCTCGCCGATCT
GGCCGCCATCGAACGGACGACAGGCGGCCGCCTGCCGGGCTGA

Upstream 100 bases:

>100_bases
GCGCTCATGGACGGCGACCTCGACGAACTGATCAGCGCGCTGGTCAGCGAACACCAGGCCGAACTGCTCGCGTCGCTCGG
CGATGCCGACTGAGGCCGCG

Downstream 100 bases:

>100_bases
TGCCCGGCCCGCCCGGTTGAAATCCGCTATCATTTCGATCTAACGCCCCGCTAACGCAAGGTCAGTCATGGACACCCAAC
AACGTATCAAGCAAATCGTC

Product: modification methylase HemK

Products: NA

Alternate protein names: M.XfaHemK2P [H]

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MPDTTADELLRASPLDAVDARVLLAHALGWTRTQLITRGDATLDAAAVERYRALEARRLAGEPVAQLVGMREFFGRPFDV
TPDVLIPRPETELLVEAALDAIDGLPHPAVLDLGTGSGAIAVSIAAERPDARVWALDRSPAALAVAQRNAGKLLDARRPG
GPLHWLESDWYAALDPALAFDTIVSNPPYIAQHDPHLAQGDLRFEPRGALTDDADGLSAIRTIVAGAGTYLKPGGTLWIE
HGYDQAEAVRAVLASHGFVAVESLADLAAIERTTGGRLPG

Sequences:

>Translated_280_residues
MPDTTADELLRASPLDAVDARVLLAHALGWTRTQLITRGDATLDAAAVERYRALEARRLAGEPVAQLVGMREFFGRPFDV
TPDVLIPRPETELLVEAALDAIDGLPHPAVLDLGTGSGAIAVSIAAERPDARVWALDRSPAALAVAQRNAGKLLDARRPG
GPLHWLESDWYAALDPALAFDTIVSNPPYIAQHDPHLAQGDLRFEPRGALTDDADGLSAIRTIVAGAGTYLKPGGTLWIE
HGYDQAEAVRAVLASHGFVAVESLADLAAIERTTGGRLPG
>Mature_279_residues
PDTTADELLRASPLDAVDARVLLAHALGWTRTQLITRGDATLDAAAVERYRALEARRLAGEPVAQLVGMREFFGRPFDVT
PDVLIPRPETELLVEAALDAIDGLPHPAVLDLGTGSGAIAVSIAAERPDARVWALDRSPAALAVAQRNAGKLLDARRPGG
PLHWLESDWYAALDPALAFDTIVSNPPYIAQHDPHLAQGDLRFEPRGALTDDADGLSAIRTIVAGAGTYLKPGGTLWIEH
GYDQAEAVRAVLASHGFVAVESLADLAAIERTTGGRLPG

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=287, Percent_Identity=30.6620209059233, Blast_Score=92, Evalue=4e-19,
Organism=Escherichia coli, GI1787463, Length=268, Percent_Identity=45.1492537313433, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI87082085, Length=201, Percent_Identity=37.3134328358209, Blast_Score=94, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24582226, Length=206, Percent_Identity=33.9805825242718, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 29714; Mature: 29583

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDTTADELLRASPLDAVDARVLLAHALGWTRTQLITRGDATLDAAAVERYRALEARRLA
CCCCCHHHHHHCCCCCHHHHHHHHHHHHCHHHHHEEECCCCHHHHHHHHHHHHHHHHHHC
GEPVAQLVGMREFFGRPFDVTPDVLIPRPETELLVEAALDAIDGLPHPAVLDLGTGSGAI
CCHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCEE
AVSIAAERPDARVWALDRSPAALAVAQRNAGKLLDARRPGGPLHWLESDWYAALDPALAF
EEEEECCCCCCEEEEECCCCCEEEEECCCCCCHHHCCCCCCCCEEECCCCHHHHCHHHHH
DTIVSNPPYIAQHDPHLAQGDLRFEPRGALTDDADGLSAIRTIVAGAGTYLKPGGTLWIE
HHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCEECCCCEEEEE
HGYDQAEAVRAVLASHGFVAVESLADLAAIERTTGGRLPG
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PDTTADELLRASPLDAVDARVLLAHALGWTRTQLITRGDATLDAAAVERYRALEARRLA
CCCCHHHHHHCCCCCHHHHHHHHHHHHCHHHHHEEECCCCHHHHHHHHHHHHHHHHHHC
GEPVAQLVGMREFFGRPFDVTPDVLIPRPETELLVEAALDAIDGLPHPAVLDLGTGSGAI
CCHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCEE
AVSIAAERPDARVWALDRSPAALAVAQRNAGKLLDARRPGGPLHWLESDWYAALDPALAF
EEEEECCCCCCEEEEECCCCCEEEEECCCCCCHHHCCCCCCCCEEECCCCHHHHCHHHHH
DTIVSNPPYIAQHDPHLAQGDLRFEPRGALTDDADGLSAIRTIVAGAGTYLKPGGTLWIE
HHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCEECCCCEEEEE
HGYDQAEAVRAVLASHGFVAVESLADLAAIERTTGGRLPG
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]