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The map label for this gene is gudD [H]

Identifier: 78064873

GI number: 78064873

Start: 253387

End: 254784

Strand: Reverse

Name: gudD [H]

Synonym: Bcep18194_A3396

Alternate gene names: 78064873

Gene position: 254784-253387 (Counterclockwise)

Preceding gene: 78064874

Following gene: 78064872

Centisome position: 6.9

GC content: 70.24

Gene sequence:

>1398_bases
ATGTCCGAATCCCGCCGTGACGGCACCCCGCGCGTCACGCGCATGCAGGTGATTCCCGTCGCCGGCCGCGACAGCATGCT
GCTCAACCTGTGCGGCGCGCACGCACCGTACTTCACGCGCAACCTCGTGATCCTCGACGACAGCAGCGGACACACGGGCG
TCGGCGAAGTGCCGGGCGGCGAAGGCATCCGCCACGCGCTCGAGCGCATGACGGATCTCGTGGTCGGCCAGTCGATCGGG
CGCTACCAGGCGACGCTCAACGCAGTGCGCGCGGCGCTGTCCGGCGCGGGTCCGGGTGCGGGCCGCACGATCCGCCACGA
GGTGACGTCGGCCGGCGAAGCAGCGGTGCTGCGCCAGCCGCACGAGATCAACCTGCGGCTCGACAACGTGATCACCGCGA
TCGAGGCGGCGCTGCTCGACCTGCTCGGCCAGCATCTCGACGTGCCGGTCGCGGCGCTGCTCGGCGAAGGGCAGCAGCGC
GACGCGGTGCCGATGCTCGCTTATCTGTTCTATATCGGCGATCGCGGCCGCACCGACCTGCCGTATCGCGACGAGGCGCA
GGCACGCACCCCGTGGTTCCGGCTGCGTAACGAGGAAGCGCTCACGCCGGCCGCGATCGCTCGGCAGGCCGAGGCCGCGG
TCGATCGCTACGGCTTCGCCGATTTCAAGCTGAAGGGCGGCGTGATGGCCGGTGCCGACGAGATGGAAGCGATCGCCGCG
ATCAAGGCGTGCTTCCCCGACGCACGCGCGACGCTCGACCCGAACGGCGCATGGTCGCTCGACGAAGCCGTCGCGCTGTG
CCGCGGACAAGGCCACCTGCTCGCGTATGCGGAGGATCCGTGCGGGCCGGAAGGTGGCTATTCGGGCCGCGAGGTGATGG
CCGAATTCCGCCGCGCGACGGGAATCCCGACCGCGACCAACATGATCGCGACCGACTGGCGGCAGATGGACCACGCGGTG
CGGCTGCAGGCGGTCGACATCCCGCTCGCCGACCCGCATTTCTGGACGATGCAGGGGTCGGTGCGGCTCGCGCAGCTGTG
CCGCGACTGGGGGCTCACGTGGGGTTCGCACTCGAACAACCACTTCGACGTGTCGCTCGCGATGTTCACGCACGCGGCGG
CAGCCGCACCCGGCACGATCACCGCGATCGACACGCACTGGATCTGGCAGGAAGGCGATGCGCGGCTCACGCGCGAGCCG
CTGAAGATCGTCGGCGGCCAGGTGGCCGTGCCGGAGCGGCCGGGGCTCGGGATCGAGCTCGACATGGCGCAGGTCGAGGC
CGCGCATGCGTTGTACAAGGAGGTCGGCGGTACGGCGCGCGACGACGCGGTCGCGATGCGCTATCTCGTGCCGGGGTGGA
CTTACGATCCTAAGCGGCCGAGTTTCGGGCGAGGGTGA

Upstream 100 bases:

>100_bases
TCGTCGTCGGCGAGATCCGCCGCGTCGAGCTTCAATGAACGGCGCGGGCCGCACCGGCGCAGCCGGCGGCCCGTGCATCA
CCGGAACCAGGAGCGAACCG

Downstream 100 bases:

>100_bases
GGGTCGCGGCGGGTAGCGGCGGGTGGTTGCCAAACGCGGAAAACGCCCGCATCCTGCCGGCTGCCTGAATCGGCCTGCAA
GGCGAACGGTTCGGCGGCCC

Product: glucarate dehydratase

Products: NA

Alternate protein names: GDH; GlucD [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MSESRRDGTPRVTRMQVIPVAGRDSMLLNLCGAHAPYFTRNLVILDDSSGHTGVGEVPGGEGIRHALERMTDLVVGQSIG
RYQATLNAVRAALSGAGPGAGRTIRHEVTSAGEAAVLRQPHEINLRLDNVITAIEAALLDLLGQHLDVPVAALLGEGQQR
DAVPMLAYLFYIGDRGRTDLPYRDEAQARTPWFRLRNEEALTPAAIARQAEAAVDRYGFADFKLKGGVMAGADEMEAIAA
IKACFPDARATLDPNGAWSLDEAVALCRGQGHLLAYAEDPCGPEGGYSGREVMAEFRRATGIPTATNMIATDWRQMDHAV
RLQAVDIPLADPHFWTMQGSVRLAQLCRDWGLTWGSHSNNHFDVSLAMFTHAAAAAPGTITAIDTHWIWQEGDARLTREP
LKIVGGQVAVPERPGLGIELDMAQVEAAHALYKEVGGTARDDAVAMRYLVPGWTYDPKRPSFGRG

Sequences:

>Translated_465_residues
MSESRRDGTPRVTRMQVIPVAGRDSMLLNLCGAHAPYFTRNLVILDDSSGHTGVGEVPGGEGIRHALERMTDLVVGQSIG
RYQATLNAVRAALSGAGPGAGRTIRHEVTSAGEAAVLRQPHEINLRLDNVITAIEAALLDLLGQHLDVPVAALLGEGQQR
DAVPMLAYLFYIGDRGRTDLPYRDEAQARTPWFRLRNEEALTPAAIARQAEAAVDRYGFADFKLKGGVMAGADEMEAIAA
IKACFPDARATLDPNGAWSLDEAVALCRGQGHLLAYAEDPCGPEGGYSGREVMAEFRRATGIPTATNMIATDWRQMDHAV
RLQAVDIPLADPHFWTMQGSVRLAQLCRDWGLTWGSHSNNHFDVSLAMFTHAAAAAPGTITAIDTHWIWQEGDARLTREP
LKIVGGQVAVPERPGLGIELDMAQVEAAHALYKEVGGTARDDAVAMRYLVPGWTYDPKRPSFGRG
>Mature_464_residues
SESRRDGTPRVTRMQVIPVAGRDSMLLNLCGAHAPYFTRNLVILDDSSGHTGVGEVPGGEGIRHALERMTDLVVGQSIGR
YQATLNAVRAALSGAGPGAGRTIRHEVTSAGEAAVLRQPHEINLRLDNVITAIEAALLDLLGQHLDVPVAALLGEGQQRD
AVPMLAYLFYIGDRGRTDLPYRDEAQARTPWFRLRNEEALTPAAIARQAEAAVDRYGFADFKLKGGVMAGADEMEAIAAI
KACFPDARATLDPNGAWSLDEAVALCRGQGHLLAYAEDPCGPEGGYSGREVMAEFRRATGIPTATNMIATDWRQMDHAVR
LQAVDIPLADPHFWTMQGSVRLAQLCRDWGLTWGSHSNNHFDVSLAMFTHAAAAAPGTITAIDTHWIWQEGDARLTREPL
KIVGGQVAVPERPGLGIELDMAQVEAAHALYKEVGGTARDDAVAMRYLVPGWTYDPKRPSFGRG

Specific function: Catalyzes the dehydration of glucarate to 5-keto-4- deoxy-D-glucarate (5-kdGluc). Also acts on L-idarate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GlucD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789150, Length=462, Percent_Identity=62.7705627705628, Blast_Score=595, Evalue=1e-171,
Organism=Escherichia coli, GI1789151, Length=456, Percent_Identity=58.7719298245614, Blast_Score=537, Evalue=1e-154,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017653
- InterPro:   IPR013342
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE [H]

EC number: =4.2.1.40 [H]

Molecular weight: Translated: 50282; Mature: 50150

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESRRDGTPRVTRMQVIPVAGRDSMLLNLCGAHAPYFTRNLVILDDSSGHTGVGEVPGG
CCCCCCCCCCCEEEEEEEEECCCCHHHHHHHCCCCCHHHCEEEEEECCCCCCCCCCCCCC
EGIRHALERMTDLVVGQSIGRYQATLNAVRAALSGAGPGAGRTIRHEVTSAGEAAVLRQP
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCHHHHCCC
HEINLRLDNVITAIEAALLDLLGQHLDVPVAALLGEGQQRDAVPMLAYLFYIGDRGRTDL
CEEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCC
PYRDEAQARTPWFRLRNEEALTPAAIARQAEAAVDRYGFADFKLKGGVMAGADEMEAIAA
CCCCCCCCCCCHHEECCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCEEECHHHHHHHHH
IKACFPDARATLDPNGAWSLDEAVALCRGQGHLLAYAEDPCGPEGGYSGREVMAEFRRAT
HHHHCCCCCCEECCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHC
GIPTATNMIATDWRQMDHAVRLQAVDIPLADPHFWTMQGSVRLAQLCRDWGLTWGSHSNN
CCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCC
HFDVSLAMFTHAAAAAPGTITAIDTHWIWQEGDARLTREPLKIVGGQVAVPERPGLGIEL
EEEEEHHHHHHHHHCCCCEEEEEEEEEEEECCCCHHHHCHHHHHCCEEECCCCCCCCEEE
DMAQVEAAHALYKEVGGTARDDAVAMRYLVPGWTYDPKRPSFGRG
HHHHHHHHHHHHHHHCCCCCCHHHHHEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SESRRDGTPRVTRMQVIPVAGRDSMLLNLCGAHAPYFTRNLVILDDSSGHTGVGEVPGG
CCCCCCCCCCEEEEEEEEECCCCHHHHHHHCCCCCHHHCEEEEEECCCCCCCCCCCCCC
EGIRHALERMTDLVVGQSIGRYQATLNAVRAALSGAGPGAGRTIRHEVTSAGEAAVLRQP
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCHHHHCCC
HEINLRLDNVITAIEAALLDLLGQHLDVPVAALLGEGQQRDAVPMLAYLFYIGDRGRTDL
CEEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCC
PYRDEAQARTPWFRLRNEEALTPAAIARQAEAAVDRYGFADFKLKGGVMAGADEMEAIAA
CCCCCCCCCCCHHEECCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCEEECHHHHHHHHH
IKACFPDARATLDPNGAWSLDEAVALCRGQGHLLAYAEDPCGPEGGYSGREVMAEFRRAT
HHHHCCCCCCEECCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHC
GIPTATNMIATDWRQMDHAVRLQAVDIPLADPHFWTMQGSVRLAQLCRDWGLTWGSHSNN
CCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCC
HFDVSLAMFTHAAAAAPGTITAIDTHWIWQEGDARLTREPLKIVGGQVAVPERPGLGIEL
EEEEEHHHHHHHHHCCCCEEEEEEEEEEEECCCCHHHHCHHHHHCCEEECCCCCCCCEEE
DMAQVEAAHALYKEVGGTARDDAVAMRYLVPGWTYDPKRPSFGRG
HHHHHHHHHHHHHHHCCCCCCHHHHHEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]