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The map label for this gene is trmE [H]

Identifier: 78050039

GI number: 78050039

Start: 5171573

End: 5172913

Strand: Reverse

Name: trmE [H]

Synonym: XCV4483

Alternate gene names: 78050039

Gene position: 5172913-5171573 (Counterclockwise)

Preceding gene: 78050040

Following gene: 78050037

Centisome position: 99.89

GC content: 65.85

Gene sequence:

>1341_bases
ATGTCGTCTTCCGCTTCGACCATCGTTGCCATCGCCAGTGCTGCCGGAATCGGCGGCGTGGGCATCGTGCGCCTGTCCGG
GGCGCAGTCGGTGCAGATTGCAGCGCAACTGGGAATTGCGCGGATGCAGCCGCGGCATGCGCACTACGCGCGTTTTCGCG
ACGGGCAGGGCGCCGTCATCGACGATGGCATTGCGCTGTGGTTCAACGCGCCGCACAGCTTTACCGGCGAAGACGTCGTC
GAGCTGCAAGGCCATGGCAGCCCGGTGCTGTTGCGGCAACTGGTTGCCCGCTGCATCGAACTGGGCGCCCGCCAGGCACG
CGCCGGTGAATTCAGTGAGCGTGCATTTCTCAACGGCAAGCTCGATCTTGCACAGGCCGAGGCGATTGCCGACTTGATCG
CAGCCGGCGACCTGCGGGCGGCACGCGCCGCGCGGCGCGCACTGGACGGGGTGTTTTCGCGACGGGTCGATGCGGTGGCG
GACACACTGACCCGCCTGCGTATCCACGTGGAAGCGGCCATCGACTTTGCCGACGAGCCGCTCGACACCTTGGGCGGCAA
CCAACTACGCGATGGTCTGGGTCAGGCGCGCAACGTGCTCGCCCAGCTGCTGCGCGATGCCGAACGCGGACGCAAGTTGC
GCGACGGCCTGCATGCGGTGTTGATCGGTCCGCCCAACGCCGGCAAGAGTTCCTTGCTCAATGCGCTGGCCGGTAGCGAT
CGGGCGATCGTCACCGATGTCGCCGGCACCACCCGCGACACCTTGCACGAAGCCATCCAGCTCGACGGCTTCGAGTTGAC
CCTGGTCGATACCGCCGGCCTGCGCGATGGCGGCGATGCGATCGAACGCGAAGGCATGCGCCGTGCACGCGCCGAGCTGG
AGCGCGCGGACCTGGCACTGGTGGTGCTGGATGCACGCGACCCGCAGGCCGCCCGCGATGCCATTGGCGATGCCATCGAT
GCCGTGCCGCGTCAGCTGTGGATCCACAACAAATGCGATCTGCTCGGCAATGCCGTATCGCTCGATGCCAATGCGATCGC
TGTCTCTGCCGTGACCGGACAGGGACTGGAGCAATTGCACATCCGCCTGCGCGCGCTTGCGCTCGGCGATGGCGTGGACA
GCGTCGAGGGCGAATTCTCTGCCCGCACACGGCATGTTCAGGCGCTGCGTCGCGCCGAACAACACGTGGACGCTGCCGAT
CTGGAACTGGGTTTCGAGCAGCTGGAACTGGCTGCCGAAGAACTGCGCCTGGCGCATGAAGCACTCGGCGAGATCACCGG
AAAGCTCAGTGCCGATGACCTGCTCGGCAAGATCTTCTCGAGTTTTTGCATCGGCAAGTGA

Upstream 100 bases:

>100_bases
GGAGGTGATTCAAAAGTGCCCGCAAATATGTGGGTCGGCGTTGTTGACTGCGCCGTTTAAACTACAGGTGTCCTTTTTCA
ATCCACGTAGAGCAGTGCCC

Downstream 100 bases:

>100_bases
TGCGGCGATGCCCAGCGCGATGAGGCGCGCGGGAACCGTCGGAACCGGGCCGCACCCAGACGCAGCTGCCGCCGCACATC
ATCTGCGTGGACGGCGATGC

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MSSSASTIVAIASAAGIGGVGIVRLSGAQSVQIAAQLGIARMQPRHAHYARFRDGQGAVIDDGIALWFNAPHSFTGEDVV
ELQGHGSPVLLRQLVARCIELGARQARAGEFSERAFLNGKLDLAQAEAIADLIAAGDLRAARAARRALDGVFSRRVDAVA
DTLTRLRIHVEAAIDFADEPLDTLGGNQLRDGLGQARNVLAQLLRDAERGRKLRDGLHAVLIGPPNAGKSSLLNALAGSD
RAIVTDVAGTTRDTLHEAIQLDGFELTLVDTAGLRDGGDAIEREGMRRARAELERADLALVVLDARDPQAARDAIGDAID
AVPRQLWIHNKCDLLGNAVSLDANAIAVSAVTGQGLEQLHIRLRALALGDGVDSVEGEFSARTRHVQALRRAEQHVDAAD
LELGFEQLELAAEELRLAHEALGEITGKLSADDLLGKIFSSFCIGK

Sequences:

>Translated_446_residues
MSSSASTIVAIASAAGIGGVGIVRLSGAQSVQIAAQLGIARMQPRHAHYARFRDGQGAVIDDGIALWFNAPHSFTGEDVV
ELQGHGSPVLLRQLVARCIELGARQARAGEFSERAFLNGKLDLAQAEAIADLIAAGDLRAARAARRALDGVFSRRVDAVA
DTLTRLRIHVEAAIDFADEPLDTLGGNQLRDGLGQARNVLAQLLRDAERGRKLRDGLHAVLIGPPNAGKSSLLNALAGSD
RAIVTDVAGTTRDTLHEAIQLDGFELTLVDTAGLRDGGDAIEREGMRRARAELERADLALVVLDARDPQAARDAIGDAID
AVPRQLWIHNKCDLLGNAVSLDANAIAVSAVTGQGLEQLHIRLRALALGDGVDSVEGEFSARTRHVQALRRAEQHVDAAD
LELGFEQLELAAEELRLAHEALGEITGKLSADDLLGKIFSSFCIGK
>Mature_445_residues
SSSASTIVAIASAAGIGGVGIVRLSGAQSVQIAAQLGIARMQPRHAHYARFRDGQGAVIDDGIALWFNAPHSFTGEDVVE
LQGHGSPVLLRQLVARCIELGARQARAGEFSERAFLNGKLDLAQAEAIADLIAAGDLRAARAARRALDGVFSRRVDAVAD
TLTRLRIHVEAAIDFADEPLDTLGGNQLRDGLGQARNVLAQLLRDAERGRKLRDGLHAVLIGPPNAGKSSLLNALAGSDR
AIVTDVAGTTRDTLHEAIQLDGFELTLVDTAGLRDGGDAIEREGMRRARAELERADLALVVLDARDPQAARDAIGDAIDA
VPRQLWIHNKCDLLGNAVSLDANAIAVSAVTGQGLEQLHIRLRALALGDGVDSVEGEFSARTRHVQALRRAEQHVDAADL
ELGFEQLELAAEELRLAHEALGEITGKLSADDLLGKIFSSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=466, Percent_Identity=39.2703862660944, Blast_Score=246, Evalue=3e-65,
Organism=Homo sapiens, GI193082988, Length=498, Percent_Identity=36.9477911646586, Blast_Score=234, Evalue=9e-62,
Organism=Homo sapiens, GI193082993, Length=455, Percent_Identity=38.021978021978, Blast_Score=233, Evalue=3e-61,
Organism=Escherichia coli, GI2367268, Length=467, Percent_Identity=48.3940042826552, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI87082120, Length=90, Percent_Identity=40, Blast_Score=76, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17507259, Length=462, Percent_Identity=32.2510822510823, Blast_Score=190, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6323665, Length=504, Percent_Identity=28.3730158730159, Blast_Score=179, Evalue=1e-45,
Organism=Drosophila melanogaster, GI45550813, Length=484, Percent_Identity=33.8842975206612, Blast_Score=208, Evalue=5e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 47290; Mature: 47159

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSASTIVAIASAAGIGGVGIVRLSGAQSVQIAAQLGIARMQPRHAHYARFRDGQGAVI
CCCCCCHHEEEEHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCEE
DDGIALWFNAPHSFTGEDVVELQGHGSPVLLRQLVARCIELGARQARAGEFSERAFLNGK
ECCEEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCHHHCCCCCHHHHHCCCC
LDLAQAEAIADLIAAGDLRAARAARRALDGVFSRRVDAVADTLTRLRIHVEAAIDFADEP
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LDTLGGNQLRDGLGQARNVLAQLLRDAERGRKLRDGLHAVLIGPPNAGKSSLLNALAGSD
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCEEEEECCCCCCHHHHHHHHCCCC
RAIVTDVAGTTRDTLHEAIQLDGFELTLVDTAGLRDGGDAIEREGMRRARAELERADLAL
CEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCEEE
VVLDARDPQAARDAIGDAIDAVPRQLWIHNKCDLLGNAVSLDANAIAVSAVTGQGLEQLH
EEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEECCCEEEEEECCCCCHHHHH
IRLRALALGDGVDSVEGEFSARTRHVQALRRAEQHVDAADLELGFEQLELAAEELRLAHE
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHHH
ALGEITGKLSADDLLGKIFSSFCIGK
HHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSSASTIVAIASAAGIGGVGIVRLSGAQSVQIAAQLGIARMQPRHAHYARFRDGQGAVI
CCCCCHHEEEEHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCEE
DDGIALWFNAPHSFTGEDVVELQGHGSPVLLRQLVARCIELGARQARAGEFSERAFLNGK
ECCEEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCHHHCCCCCHHHHHCCCC
LDLAQAEAIADLIAAGDLRAARAARRALDGVFSRRVDAVADTLTRLRIHVEAAIDFADEP
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LDTLGGNQLRDGLGQARNVLAQLLRDAERGRKLRDGLHAVLIGPPNAGKSSLLNALAGSD
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCEEEEECCCCCCHHHHHHHHCCCC
RAIVTDVAGTTRDTLHEAIQLDGFELTLVDTAGLRDGGDAIEREGMRRARAELERADLAL
CEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCEEE
VVLDARDPQAARDAIGDAIDAVPRQLWIHNKCDLLGNAVSLDANAIAVSAVTGQGLEQLH
EEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEECCCEEEEEECCCCCHHHHH
IRLRALALGDGVDSVEGEFSARTRHVQALRRAEQHVDAADLELGFEQLELAAEELRLAHE
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHHH
ALGEITGKLSADDLLGKIFSSFCIGK
HHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]