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The map label for this gene is gcvA [H]

Identifier: 78049515

GI number: 78049515

Start: 4544612

End: 4545556

Strand: Reverse

Name: gcvA [H]

Synonym: XCV3959

Alternate gene names: 78049515

Gene position: 4545556-4544612 (Counterclockwise)

Preceding gene: 78049516

Following gene: 78049514

Centisome position: 87.78

GC content: 66.98

Gene sequence:

>945_bases
ATGTCGCGTCCGCCGCTTCACGCATTGCAAGGCTTCGTCAGCGCGGTACGCCTGGGCAACCTGTCGCGCGCTGCTGGCGC
GATGCATCTGACTGTCAGCGCCCTTAGTCACCAGATGCGCGTGCTTGAACAACGGCTCGGGTATCCGCTGCTGCAACGGC
ATGCGCGCGGCGTCACTGCCACGCCGCAGGGCCAGCAACTGCTCGACCGCATCGCACCGCACCTGGACGCCATCGCCGAG
GCATTCGAGCCATTCGGTGCGCGTCGCGAAGACACCCTGACCCTGAGTCTCACGCCGTCGATGGCATCGGCCTGGCTGGT
GCCACGCCTGGGCGAATTTCTGGCCATTCATCCCACCATCGAAATCAACCTGTTGTCGAGCGAGCGGCTGGTGGATTTCG
AGCGCCAGCAGCAAGTGGATGCCGCCATGCGCATCGGCGCCGGGCAATGGCCTGGCGTCATCGCCGAGCCCTTGTTCGAC
GAATGGCTGGTGCCGATGGCAAGCCCGGCCCTGATCGCCCGCATGGGCGGCGTGGAGGCGCCACCGTTGCATCAGTGGCC
ATTGCTGGGCGACCCGGATGACGCCTGGTTGCGCTGGTTTGCCGCGTTCGGCGGCGATGTGCCGCGACGCTATGTCGCCG
TACTCGACGAATCAGAGGCACACCACCGTGCCGCCCTGGATGGCGTCGGCGTCGCACTGGGCCGCGTGACGCGCGCACGT
CTGTTGCTGGCGTCCGGCCAGCTGGTGGCGTTGTCCACGCAACGGCTCAAGACCAGTTGGTCGCATTACCTGGTGTATCC
GCCACGTTCCAGCACGCATGGCGGGTTTCTTGCGTTTCGCACCTGGCTGCAGGGCCAGGCGCGCGAACACGCCGCGCATG
CGCAGCAGGCGACCGCCATACCACTTGCCGCGCGCCGCAAGCGCTCCGGGACACTGCCGCCATGA

Upstream 100 bases:

>100_bases
GCCCACCTCCACTTGAGTGGAATCCACGGTAGGCGCAGGCTAGGGGACGTAAAAGCGAGACTTTTACAAGTGAGTCTTGA
AATCCATTCAAGGGTTGGCC

Downstream 100 bases:

>100_bases
GCCATCCACCCTTGCCACCACAATCCGAGCGCCGCGCGCTGGCGATCGCCCAGGCCGTGTACGAAGCGTTCGAGGACTAT
CACGCGCGCTTTTCCGAAAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MSRPPLHALQGFVSAVRLGNLSRAAGAMHLTVSALSHQMRVLEQRLGYPLLQRHARGVTATPQGQQLLDRIAPHLDAIAE
AFEPFGARREDTLTLSLTPSMASAWLVPRLGEFLAIHPTIEINLLSSERLVDFERQQQVDAAMRIGAGQWPGVIAEPLFD
EWLVPMASPALIARMGGVEAPPLHQWPLLGDPDDAWLRWFAAFGGDVPRRYVAVLDESEAHHRAALDGVGVALGRVTRAR
LLLASGQLVALSTQRLKTSWSHYLVYPPRSSTHGGFLAFRTWLQGQAREHAAHAQQATAIPLAARRKRSGTLPP

Sequences:

>Translated_314_residues
MSRPPLHALQGFVSAVRLGNLSRAAGAMHLTVSALSHQMRVLEQRLGYPLLQRHARGVTATPQGQQLLDRIAPHLDAIAE
AFEPFGARREDTLTLSLTPSMASAWLVPRLGEFLAIHPTIEINLLSSERLVDFERQQQVDAAMRIGAGQWPGVIAEPLFD
EWLVPMASPALIARMGGVEAPPLHQWPLLGDPDDAWLRWFAAFGGDVPRRYVAVLDESEAHHRAALDGVGVALGRVTRAR
LLLASGQLVALSTQRLKTSWSHYLVYPPRSSTHGGFLAFRTWLQGQAREHAAHAQQATAIPLAARRKRSGTLPP
>Mature_313_residues
SRPPLHALQGFVSAVRLGNLSRAAGAMHLTVSALSHQMRVLEQRLGYPLLQRHARGVTATPQGQQLLDRIAPHLDAIAEA
FEPFGARREDTLTLSLTPSMASAWLVPRLGEFLAIHPTIEINLLSSERLVDFERQQQVDAAMRIGAGQWPGVIAEPLFDE
WLVPMASPALIARMGGVEAPPLHQWPLLGDPDDAWLRWFAAFGGDVPRRYVAVLDESEAHHRAALDGVGVALGRVTRARL
LLASGQLVALSTQRLKTSWSHYLVYPPRSSTHGGFLAFRTWLQGQAREHAAHAQQATAIPLAARRKRSGTLPP

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=288, Percent_Identity=32.6388888888889, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1786448, Length=290, Percent_Identity=32.0689655172414, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI145693193, Length=302, Percent_Identity=30.4635761589404, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1788706, Length=293, Percent_Identity=28.3276450511945, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786401, Length=252, Percent_Identity=28.5714285714286, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787128, Length=309, Percent_Identity=25.8899676375405, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI157672245, Length=160, Percent_Identity=29.375, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1787589, Length=158, Percent_Identity=31.0126582278481, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790262, Length=149, Percent_Identity=31.5436241610738, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34411; Mature: 34280

Theoretical pI: Translated: 10.56; Mature: 10.56

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRPPLHALQGFVSAVRLGNLSRAAGAMHLTVSALSHQMRVLEQRLGYPLLQRHARGVTA
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCC
TPQGQQLLDRIAPHLDAIAEAFEPFGARREDTLTLSLTPSMASAWLVPRLGEFLAIHPTI
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHCCEEECCEE
EINLLSSERLVDFERQQQVDAAMRIGAGQWPGVIAEPLFDEWLVPMASPALIARMGGVEA
EEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCC
PPLHQWPLLGDPDDAWLRWFAAFGGDVPRRYVAVLDESEAHHRAALDGVGVALGRVTRAR
CCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LLLASGQLVALSTQRLKTSWSHYLVYPPRSSTHGGFLAFRTWLQGQAREHAAHAQQATAI
HHHCCCCEEEEEHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PLAARRKRSGTLPP
CHHHHHHCCCCCCC
>Mature Secondary Structure 
SRPPLHALQGFVSAVRLGNLSRAAGAMHLTVSALSHQMRVLEQRLGYPLLQRHARGVTA
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCC
TPQGQQLLDRIAPHLDAIAEAFEPFGARREDTLTLSLTPSMASAWLVPRLGEFLAIHPTI
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHCCEEECCEE
EINLLSSERLVDFERQQQVDAAMRIGAGQWPGVIAEPLFDEWLVPMASPALIARMGGVEA
EEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCC
PPLHQWPLLGDPDDAWLRWFAAFGGDVPRRYVAVLDESEAHHRAALDGVGVALGRVTRAR
CCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LLLASGQLVALSTQRLKTSWSHYLVYPPRSSTHGGFLAFRTWLQGQAREHAAHAQQATAI
HHHCCCCEEEEEHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PLAARRKRSGTLPP
CHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]