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The map label for this gene is rmd [H]

Identifier: 78049273

GI number: 78049273

Start: 4294140

End: 4295075

Strand: Reverse

Name: rmd [H]

Synonym: XCV3717

Alternate gene names: 78049273

Gene position: 4295075-4294140 (Counterclockwise)

Preceding gene: 78049274

Following gene: 78049265

Centisome position: 82.94

GC content: 59.72

Gene sequence:

>936_bases
GTGGCTTCTCATTCTGACCTGACGGGTAAGCGGGTTCTGATAAGCGGAGCGTCCGGGTTCACCGGGCGCTACATGGCCCA
GCAGCTCAAGGAACAGGGCTGCACGGTCATCGGCGTCGGAACGCGTTCGGATGGTACCGGCACGGCTGGTACCGACGAGT
TCTGGCCGATGGATCTGCGCGACGCTGCCGACGTCGCTCGCATCGTTGCGCATGCCCAAGCCGACTACGTTGTACATCTA
GCGGCAGTGGCCTTTGTCGGTCACGGGGATGCTGACGATTTTTATCGTGTCAATCTGCTCGGCACCCGGAACCTCTTGCA
GGCGTTGGCCGCAAGCCGCCACCGTCCGGAAAGGGTGCTGATCGCCAGCAGTGCCAATGTCTACGGCAATGCAACTGAGG
GCGTGATCGACGAGGCGGTCACACCCGCGCCCGCGAACGACTACGCGGTGAGCAAGCTCGCCATGGAATATGTCGCCAGC
TTGTGGCGCGAGCGGCTATCCCTGGTGATTGCCCGCCCTTTTAACTATACCGGGGTCGGTCAGGCGACCAATTTCCTGAT
TCCCAAGATCGTTTCCCATTTCGCATCTCATGCCTCTTCCATCGAGTTGGGCAACACCGAAGTGTGGCGCGATTTCGGCG
ATGTCAGATCGGTCGTGCTGGCTTACCGGAAGCTCCTGCAGGCACCGGCGGCCGAAGGGCAGATCGTCAATGTGTGTTCG
GGTGTCGCCAGTTCGCTGGGGGACATCATCGGGATCTGCTCGAAAATCACTGGCCACGACATTGACGTCCAGGTGAACCC
TGCATTCGTTCGAGAGAATGAAGTCAAGAAGTTGCTTGGCAGCAATGCCAGATTGCAGGAGCTGATCGGCGATTGGCAGA
GTCTTGCGTTAGAGGACACGCTGCGCTGGATGCTCGAAGCTAGCTGCCAGCACTAG

Upstream 100 bases:

>100_bases
GATCCCGAAAAGGCGGCCCGTATCCTCGGCTGGAAGCCTGAAACCACGCTGGAGCAGCTGTGCCAGATGATGGTCGAGGC
TGATCTGAAGAGGAACGAGC

Downstream 100 bases:

>100_bases
GACTGGGCAGTTAGAACAATCCAAGGGAATTGAAGGCAATCGAGCTTCGGCTGGAAAATTCAGCCGAAGCCCCGCCTTGC
GAATTGGTTCGGCGTTATGG

Product: NDP-hexose oxidoreductase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MASHSDLTGKRVLISGASGFTGRYMAQQLKEQGCTVIGVGTRSDGTGTAGTDEFWPMDLRDAADVARIVAHAQADYVVHL
AAVAFVGHGDADDFYRVNLLGTRNLLQALAASRHRPERVLIASSANVYGNATEGVIDEAVTPAPANDYAVSKLAMEYVAS
LWRERLSLVIARPFNYTGVGQATNFLIPKIVSHFASHASSIELGNTEVWRDFGDVRSVVLAYRKLLQAPAAEGQIVNVCS
GVASSLGDIIGICSKITGHDIDVQVNPAFVRENEVKKLLGSNARLQELIGDWQSLALEDTLRWMLEASCQH

Sequences:

>Translated_311_residues
MASHSDLTGKRVLISGASGFTGRYMAQQLKEQGCTVIGVGTRSDGTGTAGTDEFWPMDLRDAADVARIVAHAQADYVVHL
AAVAFVGHGDADDFYRVNLLGTRNLLQALAASRHRPERVLIASSANVYGNATEGVIDEAVTPAPANDYAVSKLAMEYVAS
LWRERLSLVIARPFNYTGVGQATNFLIPKIVSHFASHASSIELGNTEVWRDFGDVRSVVLAYRKLLQAPAAEGQIVNVCS
GVASSLGDIIGICSKITGHDIDVQVNPAFVRENEVKKLLGSNARLQELIGDWQSLALEDTLRWMLEASCQH
>Mature_310_residues
ASHSDLTGKRVLISGASGFTGRYMAQQLKEQGCTVIGVGTRSDGTGTAGTDEFWPMDLRDAADVARIVAHAQADYVVHLA
AVAFVGHGDADDFYRVNLLGTRNLLQALAASRHRPERVLIASSANVYGNATEGVIDEAVTPAPANDYAVSKLAMEYVASL
WRERLSLVIARPFNYTGVGQATNFLIPKIVSHFASHASSIELGNTEVWRDFGDVRSVVLAYRKLLQAPAAEGQIVNVCSG
VASSLGDIIGICSKITGHDIDVQVNPAFVRENEVKKLLGSNARLQELIGDWQSLALEDTLRWMLEASCQH

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=252, Percent_Identity=27.3809523809524, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI56237023, Length=344, Percent_Identity=25.5813953488372, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI56118217, Length=344, Percent_Identity=25.5813953488372, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI189083684, Length=344, Percent_Identity=25.5813953488372, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1788366, Length=361, Percent_Identity=26.8698060941828, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1786974, Length=293, Percent_Identity=24.9146757679181, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI133901788, Length=325, Percent_Identity=26.4615384615385, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI133901786, Length=325, Percent_Identity=26.4615384615385, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI133901790, Length=325, Percent_Identity=26.4615384615385, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17539422, Length=325, Percent_Identity=26.4615384615385, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17539424, Length=325, Percent_Identity=26.4615384615385, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17507723, Length=324, Percent_Identity=25.3086419753086, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71982035, Length=334, Percent_Identity=24.5508982035928, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71982038, Length=336, Percent_Identity=24.1071428571429, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 33523; Mature: 33392

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASHSDLTGKRVLISGASGFTGRYMAQQLKEQGCTVIGVGTRSDGTGTAGTDEFWPMDLR
CCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCHH
DAADVARIVAHAQADYVVHLAAVAFVGHGDADDFYRVNLLGTRNLLQALAASRHRPERVL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHCCCCCEEE
IASSANVYGNATEGVIDEAVTPAPANDYAVSKLAMEYVASLWRERLSLVIARPFNYTGVG
EEECCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEHCCCCCCCCCC
QATNFLIPKIVSHFASHASSIELGNTEVWRDFGDVRSVVLAYRKLLQAPAAEGQIVNVCS
CHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
GVASSLGDIIGICSKITGHDIDVQVNPAFVRENEVKKLLGSNARLQELIGDWQSLALEDT
HHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LRWMLEASCQH
HHHHHHHHCCC
>Mature Secondary Structure 
ASHSDLTGKRVLISGASGFTGRYMAQQLKEQGCTVIGVGTRSDGTGTAGTDEFWPMDLR
CCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCHH
DAADVARIVAHAQADYVVHLAAVAFVGHGDADDFYRVNLLGTRNLLQALAASRHRPERVL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHCCCCCEEE
IASSANVYGNATEGVIDEAVTPAPANDYAVSKLAMEYVASLWRERLSLVIARPFNYTGVG
EEECCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEHCCCCCCCCCC
QATNFLIPKIVSHFASHASSIELGNTEVWRDFGDVRSVVLAYRKLLQAPAAEGQIVNVCS
CHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
GVASSLGDIIGICSKITGHDIDVQVNPAFVRENEVKKLLGSNARLQELIGDWQSLALEDT
HHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LRWMLEASCQH
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]