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The map label for this gene is rmlB [H]

Identifier: 78049265

GI number: 78049265

Start: 4284693

End: 4285748

Strand: Reverse

Name: rmlB [H]

Synonym: XCV3709

Alternate gene names: 78049265

Gene position: 4285748-4284693 (Counterclockwise)

Preceding gene: 78049273

Following gene: 78049264

Centisome position: 82.76

GC content: 61.46

Gene sequence:

>1056_bases
GTGACGACTTGGCTTGTAACCGGCGGCGCCGGTTTCATCGGCGGCAATTTTGTGCTGGAAGCGGTGTCCCGCGGCGTCCG
CGTGGTCAATCTGGACGCCCTGACTTACGCGGGAAACCTGAACACACTGGCGTCGTTGGAGGGCAATCCTGACCATGTCT
TTGTCAAGGGCGATATCGGTGACGGTGCGCTCGTCACCCGCCTGCTGCAAGAACACCGGCCCGATGCGGTGCTGAATTTC
GCTGCCGAGAGCCATGTCGATCGTTCGATCGATGGGCCTGGCGCCTTCATCCAGACCAATGTCGTCGGGACCTTGGCCCT
GCTCGAAGCAGTGCGTGATTACTGGAAGGCGTTGCCGGATGCGAGTCGGGATGCGTTCCGCTTCCTGCACGTGTCCACCG
ACGAGGTCTACGGCACCCTGGGCGAGACCGGCAAGTTCACTGAGACCACGCCATATGCGCCGAACTCACCGTACTCCGCG
TCGAAAGCCGCCTCGGATCATCTGGTGCGCGCATTCCACCACACTTATGGGCTGCCGGTGCTGACCACCAATTGCTCGAA
TAATTACGGCCCATATCATTTCCCCGAAAAGCTCATCCCGCTGGTGATCGCCAAGGCGCTGGCTGGCGAGCCGCTTCCGG
TCTACGGCGACGGCAAGCAGGTGCGTGACTGGCTGTTCGTCAGCGATCACTGCGAGGCGATCCGTACGGTGCTGGGCAAC
GGTAGGGTCGGCCAAACCTACAATGTCGGTGGCAATTCCGAGCGGCAGAACATCGAGGTCGTGCAGGCCATCTGCGCATT
GCTGGATCAGCATCGTCCGCGCGAAGACGGCAAGCCACGCGCGAGCCAGATTACCCATGTGACCGACCGGCCCGGGCACG
ACCGTCGTTACGCGATCGATGCTTCGAAGTTGAAGAACGAATTGGGTTGGGAGCCTGCCTATACCTTCGAGCAGGGCATC
GCGCAGACGGTGGAGTGGTATCTTGGTAATCAGGCGTGGGTACAGGGCGTGCTGGACGGAAGCTACCGTCTGGAACGTAT
CGGCGCGGCGTCCTGA

Upstream 100 bases:

>100_bases
GCACGTGCCGCGCTTAAGCCGGATGCGGCTGGGACCGTATATCCTGTGTGGTTTAAAAAGCGCGGCACGGTTCGCGTGAC
CAGCACACAAGGGGTAAACA

Downstream 100 bases:

>100_bases
CATCCCTCTCTCTGGTGGCCGCTCAGGTGCGTGCCGACCTTCCAAGGAAGCTCTCATGACACAACGCAAAGGCATCATCC
TGGCAGGCGGCTCCGGCACC

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MTTWLVTGGAGFIGGNFVLEAVSRGVRVVNLDALTYAGNLNTLASLEGNPDHVFVKGDIGDGALVTRLLQEHRPDAVLNF
AAESHVDRSIDGPGAFIQTNVVGTLALLEAVRDYWKALPDASRDAFRFLHVSTDEVYGTLGETGKFTETTPYAPNSPYSA
SKAASDHLVRAFHHTYGLPVLTTNCSNNYGPYHFPEKLIPLVIAKALAGEPLPVYGDGKQVRDWLFVSDHCEAIRTVLGN
GRVGQTYNVGGNSERQNIEVVQAICALLDQHRPREDGKPRASQITHVTDRPGHDRRYAIDASKLKNELGWEPAYTFEQGI
AQTVEWYLGNQAWVQGVLDGSYRLERIGAAS

Sequences:

>Translated_351_residues
MTTWLVTGGAGFIGGNFVLEAVSRGVRVVNLDALTYAGNLNTLASLEGNPDHVFVKGDIGDGALVTRLLQEHRPDAVLNF
AAESHVDRSIDGPGAFIQTNVVGTLALLEAVRDYWKALPDASRDAFRFLHVSTDEVYGTLGETGKFTETTPYAPNSPYSA
SKAASDHLVRAFHHTYGLPVLTTNCSNNYGPYHFPEKLIPLVIAKALAGEPLPVYGDGKQVRDWLFVSDHCEAIRTVLGN
GRVGQTYNVGGNSERQNIEVVQAICALLDQHRPREDGKPRASQITHVTDRPGHDRRYAIDASKLKNELGWEPAYTFEQGI
AQTVEWYLGNQAWVQGVLDGSYRLERIGAAS
>Mature_350_residues
TTWLVTGGAGFIGGNFVLEAVSRGVRVVNLDALTYAGNLNTLASLEGNPDHVFVKGDIGDGALVTRLLQEHRPDAVLNFA
AESHVDRSIDGPGAFIQTNVVGTLALLEAVRDYWKALPDASRDAFRFLHVSTDEVYGTLGETGKFTETTPYAPNSPYSAS
KAASDHLVRAFHHTYGLPVLTTNCSNNYGPYHFPEKLIPLVIAKALAGEPLPVYGDGKQVRDWLFVSDHCEAIRTVLGNG
RVGQTYNVGGNSERQNIEVVQAICALLDQHRPREDGKPRASQITHVTDRPGHDRRYAIDASKLKNELGWEPAYTFEQGIA
QTVEWYLGNQAWVQGVLDGSYRLERIGAAS

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=329, Percent_Identity=35.258358662614, Blast_Score=215, Evalue=6e-56,
Organism=Homo sapiens, GI42516563, Length=330, Percent_Identity=25.4545454545455, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI4504031, Length=342, Percent_Identity=26.3157894736842, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI56237023, Length=347, Percent_Identity=26.2247838616715, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI56118217, Length=347, Percent_Identity=26.2247838616715, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI189083684, Length=347, Percent_Identity=26.2247838616715, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI48994969, Length=357, Percent_Identity=59.1036414565826, Blast_Score=420, Evalue=1e-119,
Organism=Escherichia coli, GI1788353, Length=349, Percent_Identity=58.1661891117478, Blast_Score=415, Evalue=1e-117,
Organism=Escherichia coli, GI1786974, Length=348, Percent_Identity=28.448275862069, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788366, Length=307, Percent_Identity=28.0130293159609, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17568069, Length=337, Percent_Identity=35.6083086053413, Blast_Score=218, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI115532424, Length=338, Percent_Identity=33.4319526627219, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI133901786, Length=346, Percent_Identity=26.0115606936416, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI133901788, Length=346, Percent_Identity=26.0115606936416, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI133901790, Length=346, Percent_Identity=26.0115606936416, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17539424, Length=346, Percent_Identity=26.0115606936416, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17539422, Length=346, Percent_Identity=26.0115606936416, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17507723, Length=343, Percent_Identity=24.4897959183673, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71982035, Length=370, Percent_Identity=27.027027027027, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI71982038, Length=372, Percent_Identity=26.8817204301075, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17539532, Length=330, Percent_Identity=23.030303030303, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319493, Length=358, Percent_Identity=24.5810055865922, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19923002, Length=360, Percent_Identity=27.7777777777778, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI21356223, Length=329, Percent_Identity=24.9240121580547, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24158427, Length=286, Percent_Identity=26.5734265734266, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 38330; Mature: 38199

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTWLVTGGAGFIGGNFVLEAVSRGVRVVNLDALTYAGNLNTLASLEGNPDHVFVKGDIG
CCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCEEECCCCCCEEEEEECCC
DGALVTRLLQEHRPDAVLNFAAESHVDRSIDGPGAFIQTNVVGTLALLEAVRDYWKALPD
CHHHHHHHHHHCCCCCEEHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCC
ASRDAFRFLHVSTDEVYGTLGETGKFTETTPYAPNSPYSASKAASDHLVRAFHHTYGLPV
CCCCCEEEEEECHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCE
LTTNCSNNYGPYHFPEKLIPLVIAKALAGEPLPVYGDGKQVRDWLFVSDHCEAIRTVLGN
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEECCCCHHHHHHEEEHHHHHHHHHHHCC
GRVGQTYNVGGNSERQNIEVVQAICALLDQHRPREDGKPRASQITHVTDRPGHDRRYAID
CCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCCEEEEC
ASKLKNELGWEPAYTFEQGIAQTVEWYLGNQAWVQGVLDGSYRLERIGAAS
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHCCCCC
>Mature Secondary Structure 
TTWLVTGGAGFIGGNFVLEAVSRGVRVVNLDALTYAGNLNTLASLEGNPDHVFVKGDIG
CEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCEEECCCCCCEEEEEECCC
DGALVTRLLQEHRPDAVLNFAAESHVDRSIDGPGAFIQTNVVGTLALLEAVRDYWKALPD
CHHHHHHHHHHCCCCCEEHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCC
ASRDAFRFLHVSTDEVYGTLGETGKFTETTPYAPNSPYSASKAASDHLVRAFHHTYGLPV
CCCCCEEEEEECHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCE
LTTNCSNNYGPYHFPEKLIPLVIAKALAGEPLPVYGDGKQVRDWLFVSDHCEAIRTVLGN
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEECCCCHHHHHHEEEHHHHHHHHHHHCC
GRVGQTYNVGGNSERQNIEVVQAICALLDQHRPREDGKPRASQITHVTDRPGHDRRYAID
CCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCCEEEEC
ASKLKNELGWEPAYTFEQGIAQTVEWYLGNQAWVQGVLDGSYRLERIGAAS
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]