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The map label for this gene is gmd [H]

Identifier: 78049274

GI number: 78049274

Start: 4295059

End: 4296099

Strand: Reverse

Name: gmd [H]

Synonym: XCV3718

Alternate gene names: 78049274

Gene position: 4296099-4295059 (Counterclockwise)

Preceding gene: 78049275

Following gene: 78049273

Centisome position: 82.96

GC content: 57.06

Gene sequence:

>1041_bases
ATGACTAACAAGATTGCAATCATTACCGGTATCACCGGACAGGACGGTGCTTACCTGACCGAGCTGTTGCTTGGCAAGGG
TTACAAGGTGTATGGCACATATCGACGGACCAGTTCCGTCAACTTCTGGCGCCTCGAGGAGGTTGGCGTCGCGAAACATC
CGAATCTGGAGTTGGTGGAGTATGACCTCACGGATCTGGGGACGACGATCGCGATGGTCCAGAAGATCCAGCCCGACGAG
ATCTACAATCTCGCTGCCCAGAGCTTCGTCGGAGTCAGTTTCGATCAGCCGACAACGACAGCGCAGATTACCGGTATCGG
CGCACTGCACCTGCTTGAGGCGATTCGTCTGGTGAACCCGAAGATCCGGTTCTACCAGGCATCGACTTCGGAGATGTTCG
GTAAGGTCCAGGCAGTCCCTCAAAAGGAAGACACGCCGTTTTGGCCACGTAGCCCATACGGCGTTGCTAAGCTGTATGCC
CACTGGATTACGGTGAATTATCGCGAGAGCTACGATATCTTCGGATCCAGCGGAATTCTGTTCAACCACGAAAGTCCACT
GCGCGGCCGGGAATTCGTTACCCGCAAGATCACCGACTCGGTGGCTAAGATCAAGCTCGGGCTGCTGGACTGCATGGAGT
TGGGAAACCTGGACGCCAAGCGTGACTGGGGCTTTGCGCGCGAGTATGTGGAAGGTATGTGGCGGATGCTGCAAGCCGAG
GAGCCGGACACTTTCGTGCTCGCGACAAATCGCACCGAAACCGTGCGCGATTTTGTCGGCATGGCGTTTAAGGGCGCAGG
CATCGACGTCGAGTTCCGTGGAGAGGACGCTGAAGAGACTGCGGTGGACATTGCCACCGGCAAGGTGGTCATGCGCATCA
ACTCGAAGTTCCACCGCCCTGCAGAAGTGGAGCTGCTGATCGGCGATCCCGAAAAGGCGGCCCGTATCCTCGGCTGGAAG
CCTGAAACCACGCTGGAGCAGCTGTGCCAGATGATGGTCGAGGCTGATCTGAAGAGGAACGAGCGTGGCTTCTCATTCTG
A

Upstream 100 bases:

>100_bases
GCAAGTTATGATGGCGCGCTTCCTGCACGGCGATGCTCGGGGCGCTCATGGGCGTGATCTAGAGCGGGGTTGCTTTCACA
AATATCCGAAGAGAATGTAG

Downstream 100 bases:

>100_bases
CCTGACGGGTAAGCGGGTTCTGATAAGCGGAGCGTCCGGGTTCACCGGGCGCTACATGGCCCAGCAGCTCAAGGAACAGG
GCTGCACGGTCATCGGCGTC

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase; ORF13.7 [H]

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MTNKIAIITGITGQDGAYLTELLLGKGYKVYGTYRRTSSVNFWRLEEVGVAKHPNLELVEYDLTDLGTTIAMVQKIQPDE
IYNLAAQSFVGVSFDQPTTTAQITGIGALHLLEAIRLVNPKIRFYQASTSEMFGKVQAVPQKEDTPFWPRSPYGVAKLYA
HWITVNYRESYDIFGSSGILFNHESPLRGREFVTRKITDSVAKIKLGLLDCMELGNLDAKRDWGFAREYVEGMWRMLQAE
EPDTFVLATNRTETVRDFVGMAFKGAGIDVEFRGEDAEETAVDIATGKVVMRINSKFHRPAEVELLIGDPEKAARILGWK
PETTLEQLCQMMVEADLKRNERGFSF

Sequences:

>Translated_346_residues
MTNKIAIITGITGQDGAYLTELLLGKGYKVYGTYRRTSSVNFWRLEEVGVAKHPNLELVEYDLTDLGTTIAMVQKIQPDE
IYNLAAQSFVGVSFDQPTTTAQITGIGALHLLEAIRLVNPKIRFYQASTSEMFGKVQAVPQKEDTPFWPRSPYGVAKLYA
HWITVNYRESYDIFGSSGILFNHESPLRGREFVTRKITDSVAKIKLGLLDCMELGNLDAKRDWGFAREYVEGMWRMLQAE
EPDTFVLATNRTETVRDFVGMAFKGAGIDVEFRGEDAEETAVDIATGKVVMRINSKFHRPAEVELLIGDPEKAARILGWK
PETTLEQLCQMMVEADLKRNERGFSF
>Mature_345_residues
TNKIAIITGITGQDGAYLTELLLGKGYKVYGTYRRTSSVNFWRLEEVGVAKHPNLELVEYDLTDLGTTIAMVQKIQPDEI
YNLAAQSFVGVSFDQPTTTAQITGIGALHLLEAIRLVNPKIRFYQASTSEMFGKVQAVPQKEDTPFWPRSPYGVAKLYAH
WITVNYRESYDIFGSSGILFNHESPLRGREFVTRKITDSVAKIKLGLLDCMELGNLDAKRDWGFAREYVEGMWRMLQAEE
PDTFVLATNRTETVRDFVGMAFKGAGIDVEFRGEDAEETAVDIATGKVVMRINSKFHRPAEVELLIGDPEKAARILGWKP
ETTLEQLCQMMVEADLKRNERGFSF

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=343, Percent_Identity=52.1865889212828, Blast_Score=362, Evalue=1e-100,
Organism=Escherichia coli, GI1788366, Length=351, Percent_Identity=50.997150997151, Blast_Score=361, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17539424, Length=341, Percent_Identity=52.1994134897361, Blast_Score=364, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI133901790, Length=341, Percent_Identity=52.1994134897361, Blast_Score=364, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI133901788, Length=341, Percent_Identity=52.1994134897361, Blast_Score=364, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI133901786, Length=341, Percent_Identity=52.1994134897361, Blast_Score=364, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17539422, Length=341, Percent_Identity=52.1994134897361, Blast_Score=363, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17507723, Length=341, Percent_Identity=52.4926686217009, Blast_Score=358, Evalue=2e-99,
Organism=Drosophila melanogaster, GI24158427, Length=342, Percent_Identity=50.2923976608187, Blast_Score=350, Evalue=1e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 39000; Mature: 38869

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNKIAIITGITGQDGAYLTELLLGKGYKVYGTYRRTSSVNFWRLEEVGVAKHPNLELVE
CCCCEEEEEECCCCCCHHHHHHHHCCCEEEEEEEEECCCCCEEEECCCCCCCCCCCEEEE
YDLTDLGTTIAMVQKIQPDEIYNLAAQSFVGVSFDQPTTTAQITGIGALHLLEAIRLVNP
ECHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHCCC
KIRFYQASTSEMFGKVQAVPQKEDTPFWPRSPYGVAKLYAHWITVNYRESYDIFGSSGIL
CEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHEEEEEEEEECCCCCEECCCCEE
FNHESPLRGREFVTRKITDSVAKIKLGLLDCMELGNLDAKRDWGFAREYVEGMWRMLQAE
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCC
EPDTFVLATNRTETVRDFVGMAFKGAGIDVEFRGEDAEETAVDIATGKVVMRINSKFHRP
CCCEEEEECCCHHHHHHHHHHEEECCCEEEEECCCCCHHHEEEEECCEEEEEECCCCCCC
AEVELLIGDPEKAARILGWKPETTLEQLCQMMVEADLKRNERGFSF
CEEEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TNKIAIITGITGQDGAYLTELLLGKGYKVYGTYRRTSSVNFWRLEEVGVAKHPNLELVE
CCCEEEEEECCCCCCHHHHHHHHCCCEEEEEEEEECCCCCEEEECCCCCCCCCCCEEEE
YDLTDLGTTIAMVQKIQPDEIYNLAAQSFVGVSFDQPTTTAQITGIGALHLLEAIRLVNP
ECHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHCCC
KIRFYQASTSEMFGKVQAVPQKEDTPFWPRSPYGVAKLYAHWITVNYRESYDIFGSSGIL
CEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHEEEEEEEEECCCCCEECCCCEE
FNHESPLRGREFVTRKITDSVAKIKLGLLDCMELGNLDAKRDWGFAREYVEGMWRMLQAE
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCC
EPDTFVLATNRTETVRDFVGMAFKGAGIDVEFRGEDAEETAVDIATGKVVMRINSKFHRP
CCCEEEEECCCHHHHHHHHHHEEECCCEEEEECCCCCHHHEEEEECCEEEEEECCCCCCC
AEVELLIGDPEKAARILGWKPETTLEQLCQMMVEADLKRNERGFSF
CEEEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8932701 [H]