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The map label for this gene is rluF [H]

Identifier: 78048528

GI number: 78048528

Start: 3379936

End: 3380697

Strand: Reverse

Name: rluF [H]

Synonym: XCV2972

Alternate gene names: 78048528

Gene position: 3380697-3379936 (Counterclockwise)

Preceding gene: 78048529

Following gene: 78048527

Centisome position: 65.28

GC content: 61.81

Gene sequence:

>762_bases
ATGGCGCACGGCCTGCTCCAAATCGCCGATAATTGGCAGATGACGATCCGACTCAACAAATACATCGCCGAATCCGGCTT
CTGCTCACGCCGCGAGGCCGATCGCCTGATCGGCGAACGAAGGGTCACCGTCAATGGCGTACCCGCCGGCACCGGCGCGG
TGGTCGGCGCGGAAGATACCGTGCTGGTGGATGGCCAGCCGCTACGCATACGCGAGGCCAAGAAGCTCGGTGGTCGCAAG
CACGTCTACATCGCGCTCAACAAACCGGTGGGCATCACCTGCACCACCGAGAGTTCGGTCAAGGGCAATATCGTCAAATT
CGTCGGTCACGAGCAGCGCATCTTCCCGATCGGCCGGCTCGACAAGGACTCCGAAGGGCTGATCCTGATGACCAGCAACG
GCAACATCGTCAACGAGATCCTGCGCGCGGAAAATCGCCACCAGAAGGAATATCTGGTCGCGGTCAACAAACCGGTCACC
GATGAATTCCTGCGCGGCATGGCACGCGGGGTGCGCATCCACAACGAAACCACATTGCCGTGCCGCACCGCACGCATTGC
CAAATTCGGCTTCCGCATCGTGCTAGAGCAGGGCCTGAATCGGCAGATTCGCCTGATGGCTGCCGAGTTCGGCTACCGGG
TGACGCAACTGCGCCGGGTGCGCATCGACAACATCAAACTGGCTGCGCTCAAGCCGGGGCAGTGGCGCAACCTCAGCGAT
GCGGAATTGCGCGGGCTGCTGCCGCAACGCACCGACTGGTAA

Upstream 100 bases:

>100_bases
CGCGCTTGACCCGGGCGATGTCGGGCAGCGTCAGGCCGGTGCTGCGTGTACAACGCGGCTGGCGACTGCCTGCTGCCGAT
TGCTGGCCCGGTCAGCCGGC

Downstream 100 bases:

>100_bases
GCGCCTGTGCCGGGCCGGCTGCATCGCAGTCAGGTCAGCGCAGCCGGCGTTTCGGACACGGCATATGCCCGCGCTGTAGT
GCCGTGCTGCCGCGCCAAAT

Product: pseudouridylate synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MAHGLLQIADNWQMTIRLNKYIAESGFCSRREADRLIGERRVTVNGVPAGTGAVVGAEDTVLVDGQPLRIREAKKLGGRK
HVYIALNKPVGITCTTESSVKGNIVKFVGHEQRIFPIGRLDKDSEGLILMTSNGNIVNEILRAENRHQKEYLVAVNKPVT
DEFLRGMARGVRIHNETTLPCRTARIAKFGFRIVLEQGLNRQIRLMAAEFGYRVTQLRRVRIDNIKLAALKPGQWRNLSD
AELRGLLPQRTDW

Sequences:

>Translated_253_residues
MAHGLLQIADNWQMTIRLNKYIAESGFCSRREADRLIGERRVTVNGVPAGTGAVVGAEDTVLVDGQPLRIREAKKLGGRK
HVYIALNKPVGITCTTESSVKGNIVKFVGHEQRIFPIGRLDKDSEGLILMTSNGNIVNEILRAENRHQKEYLVAVNKPVT
DEFLRGMARGVRIHNETTLPCRTARIAKFGFRIVLEQGLNRQIRLMAAEFGYRVTQLRRVRIDNIKLAALKPGQWRNLSD
AELRGLLPQRTDW
>Mature_252_residues
AHGLLQIADNWQMTIRLNKYIAESGFCSRREADRLIGERRVTVNGVPAGTGAVVGAEDTVLVDGQPLRIREAKKLGGRKH
VYIALNKPVGITCTTESSVKGNIVKFVGHEQRIFPIGRLDKDSEGLILMTSNGNIVNEILRAENRHQKEYLVAVNKPVTD
EFLRGMARGVRIHNETTLPCRTARIAKFGFRIVLEQGLNRQIRLMAAEFGYRVTQLRRVRIDNIKLAALKPGQWRNLSDA
ELRGLLPQRTDW

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=233, Percent_Identity=54.9356223175966, Blast_Score=238, Evalue=2e-64,
Organism=Escherichia coli, GI1788510, Length=238, Percent_Identity=34.453781512605, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1787524, Length=245, Percent_Identity=29.7959183673469, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI87081838, Length=177, Percent_Identity=28.2485875706215, Blast_Score=71, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 28473; Mature: 28341

Theoretical pI: Translated: 10.86; Mature: 10.86

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHGLLQIADNWQMTIRLNKYIAESGFCSRREADRLIGERRVTVNGVPAGTGAVVGAEDT
CCCCEEEEECCEEEEEEEEEHHHCCCCCCHHHHHHHHCCCEEEEECCCCCCCEEECCCCE
VLVDGQPLRIREAKKLGGRKHVYIALNKPVGITCTTESSVKGNIVKFVGHEQRIFPIGRL
EEECCCCCEEEHHHHCCCCEEEEEEECCCEEEEEECCCCCCCCEEEEECCCCEEEECCCC
DKDSEGLILMTSNGNIVNEILRAENRHQKEYLVAVNKPVTDEFLRGMARGVRIHNETTLP
CCCCCCEEEEECCCCHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCEEEECCCCCC
CRTARIAKFGFRIVLEQGLNRQIRLMAAEFGYRVTQLRRVRIDNIKLAALKPGQWRNLSD
CHHHHHHHHHHHHEEHHCCCCEEEEEEHHHCCHHHHHEEEEECCEEEEEECCCCCCCCCH
AELRGLLPQRTDW
HHHHCCCCCCCCC
>Mature Secondary Structure 
AHGLLQIADNWQMTIRLNKYIAESGFCSRREADRLIGERRVTVNGVPAGTGAVVGAEDT
CCCEEEEECCEEEEEEEEEHHHCCCCCCHHHHHHHHCCCEEEEECCCCCCCEEECCCCE
VLVDGQPLRIREAKKLGGRKHVYIALNKPVGITCTTESSVKGNIVKFVGHEQRIFPIGRL
EEECCCCCEEEHHHHCCCCEEEEEEECCCEEEEEECCCCCCCCEEEEECCCCEEEECCCC
DKDSEGLILMTSNGNIVNEILRAENRHQKEYLVAVNKPVTDEFLRGMARGVRIHNETTLP
CCCCCCEEEEECCCCHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCEEEECCCCCC
CRTARIAKFGFRIVLEQGLNRQIRLMAAEFGYRVTQLRRVRIDNIKLAALKPGQWRNLSD
CHHHHHHHHHHHHEEHHCCCCEEEEEEHHHCCHHHHHEEEEECCEEEEEECCCCCCCCCH
AELRGLLPQRTDW
HHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]