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The map label for this gene is kefB [H]

Identifier: 78048045

GI number: 78048045

Start: 2808863

End: 2810713

Strand: Reverse

Name: kefB [H]

Synonym: XCV2489

Alternate gene names: 78048045

Gene position: 2810713-2808863 (Counterclockwise)

Preceding gene: 78048046

Following gene: 78048043

Centisome position: 54.28

GC content: 64.99

Gene sequence:

>1851_bases
ATGAGCCAACCTGCCGAATCCAGTGAGCTGATCAACGTGGTAGTGCTGCTCGGCGCTGCCGTGGTGGCAATCCCGTTGTT
CCGTCGTCTGGGCCTGGGCTCGGTGTTGGGTTATCTGGCGGCGGGACTGGCAATTGGTCCGTTCGGGTTGGGCCTGTTCA
ACGAGCCGCAAGCCATCCTGCATGTGGCCGAGTTGGGCGTGGTGATGTTTCTGTTCGTGATCGGGCTGGAAATGCGCCCC
TCGCACCTGTGGAGTTTGCGCAAGGAAATCTTCGGACTCGGCACGCTGCAGATCGCGGTCTGCGCGCTGGTGTTGACCGG
CGTCGGCATGTTGCTCGGCTTTGCGCTACCGGTGGCCTTCGTGGCGGCGTCCGGTTTCGTGCTGACATCCACTGCCGTGG
TGATGCAGCTCTTGGCAGAGCGTGGCGACGTCGCCTTGCCGCCGGGGCAGAAGATCGTGGCGATCCTGCTGTTCGAAGAC
CTGTTGATCGTGCCGTTGCTGGCGATCGTGGCCTGGATGGGAGCCAGCGGCGACCCGCAGGCGGCATCGCAGCGTTGGCA
GGGAATCGGCATCGGTCTGGCCTGCATCGTCGGCTTGCTGGCGGCCGGGCGTTGGCTGCTCAATCCCTTGTTCCGCATCC
TGGCCGCATCCAAGGCGCGCGAGGTGATGACGGCAGCGGCATTGCTGGTGGTGCTGGGCGCCGCGCTGTTGATGCAGGTG
GGCGGGCTGTCGATGGCGATGGGGGCGTTCCTGGCCGGCGTGCTGTTGTCCGAGTCCACGTTCCGGCATCAGATCGAAGC
GGATATCGAACCTTTTCGCGGCATCCTGCTGGGGCTGTTTTTTCTCGGTGTCGGCATGAGCCTGGACTTGCGCGTGGTGG
CGGCGGACTGGCGCCTGATCGTGGTGGGCGTGCTGGCCCTGATGGTGGGCAAGGGCGTGTGCATCTATGCGGTGGCGCGG
CTGATGCGCAGCGACCATCGCCAGGCGCTGGACCGTGGTGTGTTGATGGCGCAAGGCGGCGAATTCGCATTCGTGCTGTT
TGCCGCCGCGTCGGCATCCGGCGTCATCGATGCCCAGGTCAATGCCAGCCTGACCGCGATCGTGGTGTTGTCGATGGCAC
TGACGCCATTGTTCGTGCTGCTGCAACGGCGGCTGACGCCGGCGGCGGCGGTCTCGCTGGATGGCGTGGAAGCAGCCAGT
GGCCAGACCGGCAGCGTGTTGATCATCGGCTTCGGGCGCTTTGGACAGGTGGCCAGTCAGTCGCTGCTGGCGCGCGACGT
GGATGTGACCATCATCGATAACGACATCGAGATGATCCAGAGCGCGGAAGAATTTGGTTTCAAGATCTACTACGGCGACG
GCACCCGGCTCGATGTGTTGCACGCCTCCGGCGCGCACAGCGCACGTGCGATTGCGGTGTGCGTCGACAACCAGGAGGCG
GCCAATCGCATCGTCGAGCTGGCAACGCGCGAGTTCCCGCATGCCAAGCTGCTGGTGCGTTCGTACGACCGCCAGCATTC
GCTGCAGTTGGTCGCTGCGGGAGTGGATTACCAGATCCGCGAGACCTTCGAGTCGGCGGTGCTCTTCGGCAAAGTGGCGC
TGGTGGAGCTGGGGGTGGACGAGGACGAAGCCGGCATGATTTCGCGTCAGATCCGCCGCCGCGATGCCGAGCGTTTCGAA
CTGGAGATTGCTGCCGGCGATGCACGCGCCGGAGCCAAGCTGATGTATGGCAACACCGTGCCGACCGCGCCCCGGCCCAC
GCCGTTCACGCGGCCGCGTCGCGGCAGCCGCACGTTGAATCCCGATGCGGTGCCGCAGGCTGAGCCAGCAGAGACACAGA
CGCGCGACTAA

Upstream 100 bases:

>100_bases
TTGCCGCGCCGGCAGAGCTCGGCTTGGCAAGCCCAAGCGGGGACGAAGATGCGCACCCTGATGCAGGCTGGGGCCGCGCA
AGTGCCGCCAGAATCCGGGC

Downstream 100 bases:

>100_bases
GGGCGGCCCACAAACCGCTGCGCAGGCGCTCCGCGGCATGGCCAGTGCGCGATGCGGTGCGCCAGTCGTCCGGCGCGATA
GCCGCGATTGGACGACTGCT

Product: glutathione-regulated potassium efflux protein B

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 616; Mature: 615

Protein sequence:

>616_residues
MSQPAESSELINVVVLLGAAVVAIPLFRRLGLGSVLGYLAAGLAIGPFGLGLFNEPQAILHVAELGVVMFLFVIGLEMRP
SHLWSLRKEIFGLGTLQIAVCALVLTGVGMLLGFALPVAFVAASGFVLTSTAVVMQLLAERGDVALPPGQKIVAILLFED
LLIVPLLAIVAWMGASGDPQAASQRWQGIGIGLACIVGLLAAGRWLLNPLFRILAASKAREVMTAAALLVVLGAALLMQV
GGLSMAMGAFLAGVLLSESTFRHQIEADIEPFRGILLGLFFLGVGMSLDLRVVAADWRLIVVGVLALMVGKGVCIYAVAR
LMRSDHRQALDRGVLMAQGGEFAFVLFAAASASGVIDAQVNASLTAIVVLSMALTPLFVLLQRRLTPAAAVSLDGVEAAS
GQTGSVLIIGFGRFGQVASQSLLARDVDVTIIDNDIEMIQSAEEFGFKIYYGDGTRLDVLHASGAHSARAIAVCVDNQEA
ANRIVELATREFPHAKLLVRSYDRQHSLQLVAAGVDYQIRETFESAVLFGKVALVELGVDEDEAGMISRQIRRRDAERFE
LEIAAGDARAGAKLMYGNTVPTAPRPTPFTRPRRGSRTLNPDAVPQAEPAETQTRD

Sequences:

>Translated_616_residues
MSQPAESSELINVVVLLGAAVVAIPLFRRLGLGSVLGYLAAGLAIGPFGLGLFNEPQAILHVAELGVVMFLFVIGLEMRP
SHLWSLRKEIFGLGTLQIAVCALVLTGVGMLLGFALPVAFVAASGFVLTSTAVVMQLLAERGDVALPPGQKIVAILLFED
LLIVPLLAIVAWMGASGDPQAASQRWQGIGIGLACIVGLLAAGRWLLNPLFRILAASKAREVMTAAALLVVLGAALLMQV
GGLSMAMGAFLAGVLLSESTFRHQIEADIEPFRGILLGLFFLGVGMSLDLRVVAADWRLIVVGVLALMVGKGVCIYAVAR
LMRSDHRQALDRGVLMAQGGEFAFVLFAAASASGVIDAQVNASLTAIVVLSMALTPLFVLLQRRLTPAAAVSLDGVEAAS
GQTGSVLIIGFGRFGQVASQSLLARDVDVTIIDNDIEMIQSAEEFGFKIYYGDGTRLDVLHASGAHSARAIAVCVDNQEA
ANRIVELATREFPHAKLLVRSYDRQHSLQLVAAGVDYQIRETFESAVLFGKVALVELGVDEDEAGMISRQIRRRDAERFE
LEIAAGDARAGAKLMYGNTVPTAPRPTPFTRPRRGSRTLNPDAVPQAEPAETQTRD
>Mature_615_residues
SQPAESSELINVVVLLGAAVVAIPLFRRLGLGSVLGYLAAGLAIGPFGLGLFNEPQAILHVAELGVVMFLFVIGLEMRPS
HLWSLRKEIFGLGTLQIAVCALVLTGVGMLLGFALPVAFVAASGFVLTSTAVVMQLLAERGDVALPPGQKIVAILLFEDL
LIVPLLAIVAWMGASGDPQAASQRWQGIGIGLACIVGLLAAGRWLLNPLFRILAASKAREVMTAAALLVVLGAALLMQVG
GLSMAMGAFLAGVLLSESTFRHQIEADIEPFRGILLGLFFLGVGMSLDLRVVAADWRLIVVGVLALMVGKGVCIYAVARL
MRSDHRQALDRGVLMAQGGEFAFVLFAAASASGVIDAQVNASLTAIVVLSMALTPLFVLLQRRLTPAAAVSLDGVEAASG
QTGSVLIIGFGRFGQVASQSLLARDVDVTIIDNDIEMIQSAEEFGFKIYYGDGTRLDVLHASGAHSARAIAVCVDNQEAA
NRIVELATREFPHAKLLVRSYDRQHSLQLVAAGVDYQIRETFESAVLFGKVALVELGVDEDEAGMISRQIRRRDAERFEL
EIAAGDARAGAKLMYGNTVPTAPRPTPFTRPRRGSRTLNPDAVPQAEPAETQTRD

Specific function: Transport system that facilitate potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=387, Percent_Identity=25.0645994832041, Blast_Score=77, Evalue=5e-14,
Organism=Escherichia coli, GI1789749, Length=557, Percent_Identity=42.7289048473968, Blast_Score=393, Evalue=1e-110,
Organism=Escherichia coli, GI1786232, Length=572, Percent_Identity=39.1608391608392, Blast_Score=391, Evalue=1e-110,
Organism=Escherichia coli, GI1786685, Length=498, Percent_Identity=28.714859437751, Blast_Score=143, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 65317; Mature: 65185

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00664 VINCULIN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPAESSELINVVVLLGAAVVAIPLFRRLGLGSVLGYLAAGLAIGPFGLGLFNEPQAIL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
HVAELGVVMFLFVIGLEMRPSHLWSLRKEIFGLGTLQIAVCALVLTGVGMLLGFALPVAF
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VAASGFVLTSTAVVMQLLAERGDVALPPGQKIVAILLFEDLLIVPLLAIVAWMGASGDPQ
HHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
AASQRWQGIGIGLACIVGLLAAGRWLLNPLFRILAASKAREVMTAAALLVVLGAALLMQV
HHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGLSMAMGAFLAGVLLSESTFRHQIEADIEPFRGILLGLFFLGVGMSLDLRVVAADWRLI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHH
VVGVLALMVGKGVCIYAVARLMRSDHRQALDRGVLMAQGGEFAFVLFAAASASGVIDAQV
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEEEECCCCCCCEEEEC
NASLTAIVVLSMALTPLFVLLQRRLTPAAAVSLDGVEAASGQTGSVLIIGFGRFGQVASQ
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHEECCCCCCCCCCCCCEEEEECCCHHHHHHH
SLLARDVDVTIIDNDIEMIQSAEEFGFKIYYGDGTRLDVLHASGAHSARAIAVCVDNQEA
HHHHCCCEEEEECCHHHHHHHHHHCCEEEEECCCCEEEEEECCCCCCCCEEEEEECCHHH
ANRIVELATREFPHAKLLVRSYDRQHSLQLVAAGVDYQIRETFESAVLFGKVALVELGVD
HHHHHHHHHHCCCHHHHHHHHHCCHHCEEEEEECCCHHHHHHHHHHHHHHHHEEEEECCC
EDEAGMISRQIRRRDAERFELEIAAGDARAGAKLMYGNTVPTAPRPTPFTRPRRGSRTLN
CCHHHHHHHHHHHCCCHHEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCC
PDAVPQAEPAETQTRD
CCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SQPAESSELINVVVLLGAAVVAIPLFRRLGLGSVLGYLAAGLAIGPFGLGLFNEPQAIL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
HVAELGVVMFLFVIGLEMRPSHLWSLRKEIFGLGTLQIAVCALVLTGVGMLLGFALPVAF
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VAASGFVLTSTAVVMQLLAERGDVALPPGQKIVAILLFEDLLIVPLLAIVAWMGASGDPQ
HHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
AASQRWQGIGIGLACIVGLLAAGRWLLNPLFRILAASKAREVMTAAALLVVLGAALLMQV
HHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGLSMAMGAFLAGVLLSESTFRHQIEADIEPFRGILLGLFFLGVGMSLDLRVVAADWRLI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHH
VVGVLALMVGKGVCIYAVARLMRSDHRQALDRGVLMAQGGEFAFVLFAAASASGVIDAQV
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEEEECCCCCCCEEEEC
NASLTAIVVLSMALTPLFVLLQRRLTPAAAVSLDGVEAASGQTGSVLIIGFGRFGQVASQ
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHEECCCCCCCCCCCCCEEEEECCCHHHHHHH
SLLARDVDVTIIDNDIEMIQSAEEFGFKIYYGDGTRLDVLHASGAHSARAIAVCVDNQEA
HHHHCCCEEEEECCHHHHHHHHHHCCEEEEECCCCEEEEEECCCCCCCCEEEEEECCHHH
ANRIVELATREFPHAKLLVRSYDRQHSLQLVAAGVDYQIRETFESAVLFGKVALVELGVD
HHHHHHHHHHCCCHHHHHHHHHCCHHCEEEEEECCCHHHHHHHHHHHHHHHHEEEEECCC
EDEAGMISRQIRRRDAERFELEIAAGDARAGAKLMYGNTVPTAPRPTPFTRPRRGSRTLN
CCHHHHHHHHHHHCCCHHEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCC
PDAVPQAEPAETQTRD
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]