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The map label for this gene is folD [H]

Identifier: 78048043

GI number: 78048043

Start: 2807311

End: 2808222

Strand: Reverse

Name: folD [H]

Synonym: XCV2487

Alternate gene names: 78048043

Gene position: 2808222-2807311 (Counterclockwise)

Preceding gene: 78048045

Following gene: 78048042

Centisome position: 54.23

GC content: 67.65

Gene sequence:

>912_bases
ATGACCGCTTCTGCGCCAGCTGCCTCCGCTTCGGCCCGCCTTCTCGATGGTCGCCGCATTGCCGAGGAATTGCTCGACGG
ATTGAAGCTGCGGGTGGATGCGCGGCTGGCCGCCGGCAAGGCGCGACCCGGGTTGGCGGTGGTGCTGGTCGGTGGCGATC
CGGCATCGTCGGTGTATGTGCGCAACAAGCGGCGCGCGGCCGAGAAGGTCGGCATCGAAGCCTTCGATTACGACCTGCCG
CAGGGCACCAGCGAAGCCCAGCTGGCGTCGCTGATCGATGAGCTCAATGCCGACCCCAAGATCCACGGCATTCTGATCCA
GCTGCCGCTGCCGGGCATCCCCGATGCCAACCGGCTGATTCAGCGCATCGACCCGCGCAAGGACGTGGACGGATTCCATC
CGCAGAATGTCGGGCACTTGGCCTTGCGCGAATTCGGGCTGCGCCCGTGCACGCCGCGCGGCATCGTGACCTTGCTCGGG
CATACCGACCAACCGGTGCGCGGCCGCAACGCCACCATCGTGGGGGTGAGCAACCACGTGGGCCGGCCGATGGGGTTGGA
GCTATTGATCGCCGGCTGCACGGTCACCAGTTGCCACAAATTCACCCCGCCCGATGTGCTGGAAGCGAGCGTGCGCAATG
CCGACATCCTGGTGGTGGCGGTGGGCCGCCCGGGCCTGATTCCGGGCGAATGGGTCAAGCCGGGCGCGGTGGTGATCGAT
GTCGGCATCAACCGGCTCGATGATGGCCGCCTGGTGGGCGACGTCGGCTTCGACGCCGCCGCGCAGCGCGCGGCCTGGAT
CACCCCGGTGCCGGGCGGGGTAGGGCCAATGACGGTGGCCACGCTGATGCAGAACACCATCGAGGCCGCCGATGCCGCCG
AGAGTGGGGATTCGGGATTGGGGATTGGTTAA

Upstream 100 bases:

>100_bases
CGCTTCGATCGAAGTGGTGCTTGAATCGGTGCGTGAGGTGGTGTCGGTCATGGCGGAATCGGGGACAATAGACGGCCTTC
GACTCTACACCTGACGCCCC

Downstream 100 bases:

>100_bases
CCGCAGCGGCCAAGCGCACGCGTCGGCCGATCCGGATTGCATCCGGGATCGGCCGGCCATCGGCATCCTGACAAACAAGC
GAACGGCGGACGGTGCAGGG

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MTASAPAASASARLLDGRRIAEELLDGLKLRVDARLAAGKARPGLAVVLVGGDPASSVYVRNKRRAAEKVGIEAFDYDLP
QGTSEAQLASLIDELNADPKIHGILIQLPLPGIPDANRLIQRIDPRKDVDGFHPQNVGHLALREFGLRPCTPRGIVTLLG
HTDQPVRGRNATIVGVSNHVGRPMGLELLIAGCTVTSCHKFTPPDVLEASVRNADILVVAVGRPGLIPGEWVKPGAVVID
VGINRLDDGRLVGDVGFDAAAQRAAWITPVPGGVGPMTVATLMQNTIEAADAAESGDSGLGIG

Sequences:

>Translated_303_residues
MTASAPAASASARLLDGRRIAEELLDGLKLRVDARLAAGKARPGLAVVLVGGDPASSVYVRNKRRAAEKVGIEAFDYDLP
QGTSEAQLASLIDELNADPKIHGILIQLPLPGIPDANRLIQRIDPRKDVDGFHPQNVGHLALREFGLRPCTPRGIVTLLG
HTDQPVRGRNATIVGVSNHVGRPMGLELLIAGCTVTSCHKFTPPDVLEASVRNADILVVAVGRPGLIPGEWVKPGAVVID
VGINRLDDGRLVGDVGFDAAAQRAAWITPVPGGVGPMTVATLMQNTIEAADAAESGDSGLGIG
>Mature_302_residues
TASAPAASASARLLDGRRIAEELLDGLKLRVDARLAAGKARPGLAVVLVGGDPASSVYVRNKRRAAEKVGIEAFDYDLPQ
GTSEAQLASLIDELNADPKIHGILIQLPLPGIPDANRLIQRIDPRKDVDGFHPQNVGHLALREFGLRPCTPRGIVTLLGH
TDQPVRGRNATIVGVSNHVGRPMGLELLIAGCTVTSCHKFTPPDVLEASVRNADILVVAVGRPGLIPGEWVKPGAVVIDV
GINRLDDGRLVGDVGFDAAAQRAAWITPVPGGVGPMTVATLMQNTIEAADAAESGDSGLGIG

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=292, Percent_Identity=43.8356164383562, Blast_Score=221, Evalue=5e-58,
Organism=Homo sapiens, GI94721354, Length=297, Percent_Identity=43.4343434343434, Blast_Score=221, Evalue=8e-58,
Organism=Homo sapiens, GI222418558, Length=293, Percent_Identity=41.9795221843003, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI36796743, Length=208, Percent_Identity=25.4807692307692, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1786741, Length=280, Percent_Identity=58.9285714285714, Blast_Score=332, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI17568735, Length=256, Percent_Identity=42.578125, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6319558, Length=296, Percent_Identity=40.5405405405405, Blast_Score=206, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6321643, Length=297, Percent_Identity=41.0774410774411, Blast_Score=202, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6322933, Length=288, Percent_Identity=25.6944444444444, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17136816, Length=296, Percent_Identity=46.9594594594595, Blast_Score=243, Evalue=1e-64,
Organism=Drosophila melanogaster, GI17136818, Length=292, Percent_Identity=47.2602739726027, Blast_Score=241, Evalue=4e-64,
Organism=Drosophila melanogaster, GI62472483, Length=299, Percent_Identity=42.1404682274247, Blast_Score=208, Evalue=4e-54,
Organism=Drosophila melanogaster, GI45551871, Length=299, Percent_Identity=42.1404682274247, Blast_Score=208, Evalue=4e-54,
Organism=Drosophila melanogaster, GI24645718, Length=291, Percent_Identity=42.2680412371134, Blast_Score=207, Evalue=7e-54,
Organism=Drosophila melanogaster, GI17137370, Length=291, Percent_Identity=42.2680412371134, Blast_Score=207, Evalue=7e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 31630; Mature: 31499

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASAPAASASARLLDGRRIAEELLDGLKLRVDARLAAGKARPGLAVVLVGGDPASSVYV
CCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEEEEECCCCCCCEEE
RNKRRAAEKVGIEAFDYDLPQGTSEAQLASLIDELNADPKIHGILIQLPLPGIPDANRLI
ECHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHH
QRIDPRKDVDGFHPQNVGHLALREFGLRPCTPRGIVTLLGHTDQPVRGRNATIVGVSNHV
HHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECHHCC
GRPMGLELLIAGCTVTSCHKFTPPDVLEASVRNADILVVAVGRPGLIPGEWVKPGAVVID
CCCCCEEEEEECCCEECCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEE
VGINRLDDGRLVGDVGFDAAAQRAAWITPVPGGVGPMTVATLMQNTIEAADAAESGDSGL
ECCCCCCCCEEEEECCCCHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
GIG
CCC
>Mature Secondary Structure 
TASAPAASASARLLDGRRIAEELLDGLKLRVDARLAAGKARPGLAVVLVGGDPASSVYV
CCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEEEEECCCCCCCEEE
RNKRRAAEKVGIEAFDYDLPQGTSEAQLASLIDELNADPKIHGILIQLPLPGIPDANRLI
ECHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHH
QRIDPRKDVDGFHPQNVGHLALREFGLRPCTPRGIVTLLGHTDQPVRGRNATIVGVSNHV
HHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECHHCC
GRPMGLELLIAGCTVTSCHKFTPPDVLEASVRNADILVVAVGRPGLIPGEWVKPGAVVID
CCCCCEEEEEECCCEECCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEE
VGINRLDDGRLVGDVGFDAAAQRAAWITPVPGGVGPMTVATLMQNTIEAADAAESGDSGL
ECCCCCCCCEEEEECCCCHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
GIG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]