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The map label for this gene is alkH [H]

Identifier: 78047789

GI number: 78047789

Start: 2564921

End: 2565580

Strand: Reverse

Name: alkH [H]

Synonym: XCV2233

Alternate gene names: 78047789

Gene position: 2565580-2564921 (Counterclockwise)

Preceding gene: 78047790

Following gene: 78047785

Centisome position: 49.54

GC content: 69.39

Gene sequence:

>660_bases
ATGACGATTGCCCAGACCCAGAACACCGCCGAACGTTTGCTGCGCGATGCCGGCATCCTGCCGGTGGTCACCGTGGACAC
GCTGGACCAGGCGCGCCGCGTCGCCGATGCGCTGCTCGAAGGCGGTCTGCCCGCGATCGAGCTGACCCTGCGCACGCCGG
TTGCCATCGATGCGCTGGCGATGCTCAAGCGCGAGCTGCCCAACATCGTGATCGGCGCCGGCACGGTGCTCAGCGAGCTG
CAACTGCGCCAGTCGGTGGATGCCGGTGCAGACTTTCTGGTGACCCCGGGCACGCCGGCGCCGCTGGCGCGCCTGCTGGC
GGATGCACCGATCCCGGCGGTTCCAGGCGCGGCCACCCCGACCGAGCTGCTGACGTTGATGGGCCTGGGCTTTCGCGTCT
GCAAGTTGTTCCCGGCCACCGCCGTGGGCGGTCTGCAGATGCTCAAGGGCCTGGCTGGCCCGTTGTCCGAGCTCAAGCTC
TGCCCCACCGGCGGCATCAGCGAGTCCAACGCCGCCGAGTTCCTGGCGCAGCCGAACGTGCTGTGCATCGGCGGCTCGTG
GATGGTGCCCAAGGACTGGCTGGCGCAGGGCCAGTGGGACAAGGTCAAGGAAAGTTCGGCCAAGGCGGCGGCGATCGTGC
GGCAGGTGCGTGCAGGCTGA

Upstream 100 bases:

>100_bases
GCCGATGCTGCCGCTGCGGCAGCACCCCACGAGTCCAAGGACGCCTGATCCCCTTCCCTCTGTGCGGCCGCTCCGGCGGT
CGCCGTCACCCGGAGTTGCA

Downstream 100 bases:

>100_bases
CGCGCTGACGCCATCTCCAGCGGGTTGATCGTTGGATGACTTCGAATCTGTACGCCGATACAGATGTACGGATTCGCTAA
GGACGGCATTGGAGATCGAG

Product: keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MTIAQTQNTAERLLRDAGILPVVTVDTLDQARRVADALLEGGLPAIELTLRTPVAIDALAMLKRELPNIVIGAGTVLSEL
QLRQSVDAGADFLVTPGTPAPLARLLADAPIPAVPGAATPTELLTLMGLGFRVCKLFPATAVGGLQMLKGLAGPLSELKL
CPTGGISESNAAEFLAQPNVLCIGGSWMVPKDWLAQGQWDKVKESSAKAAAIVRQVRAG

Sequences:

>Translated_219_residues
MTIAQTQNTAERLLRDAGILPVVTVDTLDQARRVADALLEGGLPAIELTLRTPVAIDALAMLKRELPNIVIGAGTVLSEL
QLRQSVDAGADFLVTPGTPAPLARLLADAPIPAVPGAATPTELLTLMGLGFRVCKLFPATAVGGLQMLKGLAGPLSELKL
CPTGGISESNAAEFLAQPNVLCIGGSWMVPKDWLAQGQWDKVKESSAKAAAIVRQVRAG
>Mature_218_residues
TIAQTQNTAERLLRDAGILPVVTVDTLDQARRVADALLEGGLPAIELTLRTPVAIDALAMLKRELPNIVIGAGTVLSELQ
LRQSVDAGADFLVTPGTPAPLARLLADAPIPAVPGAATPTELLTLMGLGFRVCKLFPATAVGGLQMLKGLAGPLSELKLC
PTGGISESNAAEFLAQPNVLCIGGSWMVPKDWLAQGQWDKVKESSAKAAAIVRQVRAG

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=203, Percent_Identity=41.871921182266, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI48994954, Length=200, Percent_Identity=29, Blast_Score=64, Evalue=6e-12,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 22788; Mature: 22657

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIAQTQNTAERLLRDAGILPVVTVDTLDQARRVADALLEGGLPAIELTLRTPVAIDALA
CCCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
MLKRELPNIVIGAGTVLSELQLRQSVDAGADFLVTPGTPAPLARLLADAPIPAVPGAATP
HHHHHCCCEEEECHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHCCCCCCCCCCCCH
TELLTLMGLGFRVCKLFPATAVGGLQMLKGLAGPLSELKLCPTGGISESNAAEFLAQPNV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCEECCCCCCCCCHHHHHHCCCCE
LCIGGSWMVPKDWLAQGQWDKVKESSAKAAAIVRQVRAG
EEECCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TIAQTQNTAERLLRDAGILPVVTVDTLDQARRVADALLEGGLPAIELTLRTPVAIDALA
CCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
MLKRELPNIVIGAGTVLSELQLRQSVDAGADFLVTPGTPAPLARLLADAPIPAVPGAATP
HHHHHCCCEEEECHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHCCCCCCCCCCCCH
TELLTLMGLGFRVCKLFPATAVGGLQMLKGLAGPLSELKLCPTGGISESNAAEFLAQPNV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCEECCCCCCCCCHHHHHHCCCCE
LCIGGSWMVPKDWLAQGQWDKVKESSAKAAAIVRQVRAG
EEECCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8145647 [H]