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The map label for this gene is ndhF [H]

Identifier: 78047733

GI number: 78047733

Start: 2503979

End: 2505580

Strand: Reverse

Name: ndhF [H]

Synonym: XCV2177

Alternate gene names: 78047733

Gene position: 2505580-2503979 (Counterclockwise)

Preceding gene: 78047735

Following gene: 78047732

Centisome position: 48.38

GC content: 69.16

Gene sequence:

>1602_bases
TTGTCGCTCTATCTACTCCCGCTGTTCGCACCACTCCTGTTGCTGACCGCCGCTGTCGTCGCCTTGGGGCGCGGCGGTCG
GCCTCCACTGAAGATTCCCCTCCTCGCCGAAGGCGCCGCGCTCGGCGCGCTCTGCATCGCGATCGCATCGCTGGCGCTGG
TGCTCCTGCGAGGAACCGGCGCCAGCCCGCTGATCGGATGGGGCGATATCGGCCTGTCCGCGCGGCTGGACGCGGTCAGC
GTGACGATGCTCCTGCTGGTGGCCTTCGTTGGCTGGATCGTGGTGCGGTACGCGCGCACCTATCTCGACGGCGAGGCACG
CCAGGGCTATTTCACCGGCTGGCTGTGCATGGCGCTGGCGGCAGTGCTGCTGCTGGTGCAGGCCGGCAACCTGGTCCAGG
TCGTCATCGCCTGGATCGCCACCAGCATCGCGATGCATCGCCTGCTGCTGTTCTATCCCGAGCGCGTTGAGGCGCAGCGC
GCCGCACGCAAGAAGCGCGTGTTCTCCACCACCGGCGGTATCGCCCTGACCTGCGGCGCGGTGCTGATCTGGAAAAGCAT
GGGCACGACCGATATCGCCACGATCAATGCGCTGGCCGCGCGCGGGCAGCACTCCTGGGCGCTGGCCGCGCGCGGGCAGC
ACTCCTGGGCGCTGGTCGCGGCGGCCGCCCTGATCGCACTCGCGGCGGTGCTGAAATCGGCGCAATTCCCGACCCACGGC
TGGCTCACCGAGATGATGGAAGCGCCCACGCCGGTATCGGCCTTGCTGCACGCCGGCGTGGTCAACGGCGGCGGCTTCCT
GCTGATCCGCTTTGCCGATCTGATGCTCGCCGCCCCCGGCGTGCTCGCCGTGCTGGCCATGCTGGGCGGGTTCACCGCCT
TGTTCGGCGCGCTAGTGATGCTGACCCAGCCGGCGGTCAAGACCTCGCTGGCCTGGTCCACGGTCGCGCAGATGGGATTC
ATGATGCTCCAGTGCGGTCTGGCGCTGTTTCCGCTGGCCCTGCTGCACATCGTGGCGCACTCGCTCTACAAGGCGCACGC
GTTCCTGGCCTCCGGCGGCGCGGTCGAGCAAGTGGCGGCCATTCGCCGGCCCGGGCCGGTCGCCGTTGCGAACGGGTCGG
CGGTCGGCCGCGCATTTTTGATCGCACTGGCGATCTATATCGGCATCGGCACTGCCTTCGGGTTCACCTTCGGCTTCGAG
CACAAGTCGCCACAGGCGCTGGCACTGGGCGCGATCCTGATCTTTGGTGTCGCCTACCTGCTGGCCCAGGGCCTTGCCGA
TGCCGCGCCACGCCCTCTCACCCGCATGACGGCGATCTACGCCAGTGCCGCAGCGATCGGCTACTTCGCGCTGCAGACCG
CTGCCGAATGGCTGACGGCCGGCGTGCTGCCGGGCACGCCCGCACCGGGGCGGCTGGAGTGGACGCTGATGACGCTTGCG
GTACTGAGCTTCGGGCTGGTGGCGGTGGCGCAGGCGCTGTTCCCGCTCTGGGCCTCGCACCCCGCGGCCGCCGGGCTCCG
CGTCCACCTCTCCAATGGGCTGTATGCCAACGCCACGATCGATCGCCTGCTCGGCGGCTGGTCCATTCGCAAGACCTCCT
GA

Upstream 100 bases:

>100_bases
TAATAACGAACGAATCTGCAAGAACAATGAATTTTTATTCAACCCCACCGATCGCTATCGTGGCGCGGCCCGCCACCACG
TTCGTTCTTAGGGGATTGCC

Downstream 100 bases:

>100_bases
ACCCCTTCACCCCAGGCCCCCACGCTGATGACCACCACGCCCACCGCCATGCCTGTGTCCCCGTCCCACGATGTGATCAT
CGCCGCCGCCCAACGTGCGG

Product: NADH dehydrogenase (ubiquinone)

Products: NAD+; ubiquinol [C]

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 533; Mature: 532

Protein sequence:

>533_residues
MSLYLLPLFAPLLLLTAAVVALGRGGRPPLKIPLLAEGAALGALCIAIASLALVLLRGTGASPLIGWGDIGLSARLDAVS
VTMLLLVAFVGWIVVRYARTYLDGEARQGYFTGWLCMALAAVLLLVQAGNLVQVVIAWIATSIAMHRLLLFYPERVEAQR
AARKKRVFSTTGGIALTCGAVLIWKSMGTTDIATINALAARGQHSWALAARGQHSWALVAAAALIALAAVLKSAQFPTHG
WLTEMMEAPTPVSALLHAGVVNGGGFLLIRFADLMLAAPGVLAVLAMLGGFTALFGALVMLTQPAVKTSLAWSTVAQMGF
MMLQCGLALFPLALLHIVAHSLYKAHAFLASGGAVEQVAAIRRPGPVAVANGSAVGRAFLIALAIYIGIGTAFGFTFGFE
HKSPQALALGAILIFGVAYLLAQGLADAAPRPLTRMTAIYASAAAIGYFALQTAAEWLTAGVLPGTPAPGRLEWTLMTLA
VLSFGLVAVAQALFPLWASHPAAAGLRVHLSNGLYANATIDRLLGGWSIRKTS

Sequences:

>Translated_533_residues
MSLYLLPLFAPLLLLTAAVVALGRGGRPPLKIPLLAEGAALGALCIAIASLALVLLRGTGASPLIGWGDIGLSARLDAVS
VTMLLLVAFVGWIVVRYARTYLDGEARQGYFTGWLCMALAAVLLLVQAGNLVQVVIAWIATSIAMHRLLLFYPERVEAQR
AARKKRVFSTTGGIALTCGAVLIWKSMGTTDIATINALAARGQHSWALAARGQHSWALVAAAALIALAAVLKSAQFPTHG
WLTEMMEAPTPVSALLHAGVVNGGGFLLIRFADLMLAAPGVLAVLAMLGGFTALFGALVMLTQPAVKTSLAWSTVAQMGF
MMLQCGLALFPLALLHIVAHSLYKAHAFLASGGAVEQVAAIRRPGPVAVANGSAVGRAFLIALAIYIGIGTAFGFTFGFE
HKSPQALALGAILIFGVAYLLAQGLADAAPRPLTRMTAIYASAAAIGYFALQTAAEWLTAGVLPGTPAPGRLEWTLMTLA
VLSFGLVAVAQALFPLWASHPAAAGLRVHLSNGLYANATIDRLLGGWSIRKTS
>Mature_532_residues
SLYLLPLFAPLLLLTAAVVALGRGGRPPLKIPLLAEGAALGALCIAIASLALVLLRGTGASPLIGWGDIGLSARLDAVSV
TMLLLVAFVGWIVVRYARTYLDGEARQGYFTGWLCMALAAVLLLVQAGNLVQVVIAWIATSIAMHRLLLFYPERVEAQRA
ARKKRVFSTTGGIALTCGAVLIWKSMGTTDIATINALAARGQHSWALAARGQHSWALVAAAALIALAAVLKSAQFPTHGW
LTEMMEAPTPVSALLHAGVVNGGGFLLIRFADLMLAAPGVLAVLAMLGGFTALFGALVMLTQPAVKTSLAWSTVAQMGFM
MLQCGLALFPLALLHIVAHSLYKAHAFLASGGAVEQVAAIRRPGPVAVANGSAVGRAFLIALAIYIGIGTAFGFTFGFEH
KSPQALALGAILIFGVAYLLAQGLADAAPRPLTRMTAIYASAAAIGYFALQTAAEWLTAGVLPGTPAPGRLEWTLMTLAV
LSFGLVAVAQALFPLWASHPAAAGLRVHLSNGLYANATIDRLLGGWSIRKTS

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=312, Percent_Identity=32.3717948717949, Blast_Score=132, Evalue=1e-30,
Organism=Escherichia coli, GI1788614, Length=303, Percent_Identity=33.3333333333333, Blast_Score=138, Evalue=9e-34,
Organism=Escherichia coli, GI1788829, Length=307, Percent_Identity=29.9674267100977, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788831, Length=308, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 55477; Mature: 55346

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLYLLPLFAPLLLLTAAVVALGRGGRPPLKIPLLAEGAALGALCIAIASLALVLLRGTG
CCEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCC
ASPLIGWGDIGLSARLDAVSVTMLLLVAFVGWIVVRYARTYLDGEARQGYFTGWLCMALA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
AVLLLVQAGNLVQVVIAWIATSIAMHRLLLFYPERVEAQRAARKKRVFSTTGGIALTCGA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
VLIWKSMGTTDIATINALAARGQHSWALAARGQHSWALVAAAALIALAAVLKSAQFPTHG
HHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHH
WLTEMMEAPTPVSALLHAGVVNGGGFLLIRFADLMLAAPGVLAVLAMLGGFTALFGALVM
HHHHHHCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
LTQPAVKTSLAWSTVAQMGFMMLQCGLALFPLALLHIVAHSLYKAHAFLASGGAVEQVAA
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
IRRPGPVAVANGSAVGRAFLIALAIYIGIGTAFGFTFGFEHKSPQALALGAILIFGVAYL
HCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
LAQGLADAAPRPLTRMTAIYASAAAIGYFALQTAAEWLTAGVLPGTPAPGRLEWTLMTLA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
VLSFGLVAVAQALFPLWASHPAAAGLRVHLSNGLYANATIDRLLGGWSIRKTS
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SLYLLPLFAPLLLLTAAVVALGRGGRPPLKIPLLAEGAALGALCIAIASLALVLLRGTG
CEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCC
ASPLIGWGDIGLSARLDAVSVTMLLLVAFVGWIVVRYARTYLDGEARQGYFTGWLCMALA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
AVLLLVQAGNLVQVVIAWIATSIAMHRLLLFYPERVEAQRAARKKRVFSTTGGIALTCGA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
VLIWKSMGTTDIATINALAARGQHSWALAARGQHSWALVAAAALIALAAVLKSAQFPTHG
HHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHH
WLTEMMEAPTPVSALLHAGVVNGGGFLLIRFADLMLAAPGVLAVLAMLGGFTALFGALVM
HHHHHHCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
LTQPAVKTSLAWSTVAQMGFMMLQCGLALFPLALLHIVAHSLYKAHAFLASGGAVEQVAA
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
IRRPGPVAVANGSAVGRAFLIALAIYIGIGTAFGFTFGFEHKSPQALALGAILIFGVAYL
HCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
LAQGLADAAPRPLTRMTAIYASAAAIGYFALQTAAEWLTAGVLPGTPAPGRLEWTLMTLA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
VLSFGLVAVAQALFPLWASHPAAAGLRVHLSNGLYANATIDRLLGGWSIRKTS
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]