Click here to switch to the map view.

The map label for this gene is thcD [H]

Identifier: 78047735

GI number: 78047735

Start: 2506737

End: 2507957

Strand: Reverse

Name: thcD [H]

Synonym: XCV2179

Alternate gene names: 78047735

Gene position: 2507957-2506737 (Counterclockwise)

Preceding gene: 78047736

Following gene: 78047733

Centisome position: 48.43

GC content: 68.14

Gene sequence:

>1221_bases
ATGAGCGGCATGGTCATCGTCGGTGCGGGTCAGGCGGGCCTGCAGACCGCCGAATCCCTGCGCAGCGGCGGCTATACGGG
CTCCATCGTCTTATTGGGGGACGAGCCCTGCGCGCCCTATCACCGTCCATCGCTCTCCAAGGGGTTCCTGCTTGGCGAGG
TGAGCGACACCCAGCTGTACATTCGCGCACCCGGGGCCTTGAACCGCAAGGGCATCCAGTGGCATGCCAGCGCGCGGGTG
ATGCGCATCGAACACGCCACCCGCACGCTGCACCTGGACGACGGCAGCACCCTGGGCTACACGGGGCTGTGTCTTGCCAC
CGGCGCACGCGCCCGCCGGCTGGAGGTCCCAGGCGCCGGGCTCGGCCATGTCTGCATGTTGCGCGGCATGGCCGACACTC
GCGCGCTTGCCGCGATCCTGCCGCACACCTCGCGGGTGGTGGTGATCGGTGGCGGCTTCATCGGGCTGGAATTCGCCTCC
ATCGCCAGGCGGCTCGGCAAGCAGGTCGTTGTGCTGGAGGCGGCCGATCGGCTGATGGCGCGCGTCGTCAGCCCGCAACT
GGCGGACTTCTTCCTTCGCCTGCATCGAGACAACGGCGCGACCATCGAACTGGGCAGCAACGTCAGCGCATTGAGCGGCA
ATCGCGGCGTGGTGACTGCCGTCCACACCGCGGACGGGCGCGTGTTCCCGGCAGACCTGGTCGTCGTCGGGATCGGCGTC
ATCCCCAACGGCGAACTGGCCCAACAGGCCGGACTGGCTTGCGATCGCGGGGCATTGATCGTGGACGCGTGCGCACGCAG
TAGCAGCCCCGGCATCGTCGGTGCCGGCGACTGCACGGTGCGGCAGCGGGCAGGCAGCGGTTTGCTGCGGCTGGAGTCGG
TCCAGAATGCAATCGAACAAGCCAAATCCGCTGCCGCCTCACTGCTGGGCGAGCACCGCCCCTACCCTGCGTTGCCCTGG
TTCTGGTCGGAGCAGTACGAGGTCAGGCTGCAAATGGCCGGCTTTGCCGCAGGCCACACGCAGGCGGTGGTGCGCGGCGA
CCTGGCGACCAGCACGTTCTCCCTCTTCTACTACGTCGATGGCGAACTCTGCGCCGTGGACTCGATCAATCGGCCACAGG
ATCATCTGGCGGCCCGCAAGCTGCTCGAACTGGGACGCTCGCCGACGCCGCAGCAAGCCGCCGACCCCAACTTCGCGTTG
ACCAGCCTCATCCCCGCCTAG

Upstream 100 bases:

>100_bases
CGCAGCAACAGCCGGCTGGCGTGCCAGCTCGTACCGACATCGGACATGACCGTACGCTTTCCGGATCGGCAGACATGAGC
CTGCGTACGGCAGGTGCGCG

Downstream 100 bases:

>100_bases
CAATGCGGCTGGTCGATCCGGCTCGAGCCCGCGCGCCTGCGCTTCGGCGGTGCGGCTGCACGCGCGCCTTGCGTTCAAGA
CCGCACTGCAGCGGCTTGGC

Product: putative NAD(FAD)-dependent oxidoreductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MSGMVIVGAGQAGLQTAESLRSGGYTGSIVLLGDEPCAPYHRPSLSKGFLLGEVSDTQLYIRAPGALNRKGIQWHASARV
MRIEHATRTLHLDDGSTLGYTGLCLATGARARRLEVPGAGLGHVCMLRGMADTRALAAILPHTSRVVVIGGGFIGLEFAS
IARRLGKQVVVLEAADRLMARVVSPQLADFFLRLHRDNGATIELGSNVSALSGNRGVVTAVHTADGRVFPADLVVVGIGV
IPNGELAQQAGLACDRGALIVDACARSSSPGIVGAGDCTVRQRAGSGLLRLESVQNAIEQAKSAAASLLGEHRPYPALPW
FWSEQYEVRLQMAGFAAGHTQAVVRGDLATSTFSLFYYVDGELCAVDSINRPQDHLAARKLLELGRSPTPQQAADPNFAL
TSLIPA

Sequences:

>Translated_406_residues
MSGMVIVGAGQAGLQTAESLRSGGYTGSIVLLGDEPCAPYHRPSLSKGFLLGEVSDTQLYIRAPGALNRKGIQWHASARV
MRIEHATRTLHLDDGSTLGYTGLCLATGARARRLEVPGAGLGHVCMLRGMADTRALAAILPHTSRVVVIGGGFIGLEFAS
IARRLGKQVVVLEAADRLMARVVSPQLADFFLRLHRDNGATIELGSNVSALSGNRGVVTAVHTADGRVFPADLVVVGIGV
IPNGELAQQAGLACDRGALIVDACARSSSPGIVGAGDCTVRQRAGSGLLRLESVQNAIEQAKSAAASLLGEHRPYPALPW
FWSEQYEVRLQMAGFAAGHTQAVVRGDLATSTFSLFYYVDGELCAVDSINRPQDHLAARKLLELGRSPTPQQAADPNFAL
TSLIPA
>Mature_405_residues
SGMVIVGAGQAGLQTAESLRSGGYTGSIVLLGDEPCAPYHRPSLSKGFLLGEVSDTQLYIRAPGALNRKGIQWHASARVM
RIEHATRTLHLDDGSTLGYTGLCLATGARARRLEVPGAGLGHVCMLRGMADTRALAAILPHTSRVVVIGGGFIGLEFASI
ARRLGKQVVVLEAADRLMARVVSPQLADFFLRLHRDNGATIELGSNVSALSGNRGVVTAVHTADGRVFPADLVVVGIGVI
PNGELAQQAGLACDRGALIVDACARSSSPGIVGAGDCTVRQRAGSGLLRLESVQNAIEQAKSAAASLLGEHRPYPALPWF
WSEQYEVRLQMAGFAAGHTQAVVRGDLATSTFSLFYYVDGELCAVDSINRPQDHLAARKLLELGRSPTPQQAADPNFALT
SLIPA

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=372, Percent_Identity=29.3010752688172, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI226437568, Length=372, Percent_Identity=29.3010752688172, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI21389617, Length=372, Percent_Identity=29.3010752688172, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI22202629, Length=339, Percent_Identity=25.3687315634218, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI4757732, Length=339, Percent_Identity=25.3687315634218, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1788892, Length=401, Percent_Identity=33.9152119700748, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1789065, Length=320, Percent_Identity=28.125, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1789765, Length=280, Percent_Identity=26.4285714285714, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17559934, Length=369, Percent_Identity=27.3712737127371, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24639257, Length=400, Percent_Identity=26, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI281359715, Length=400, Percent_Identity=26, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI281359713, Length=400, Percent_Identity=26, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24639250, Length=400, Percent_Identity=26, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI18543267, Length=400, Percent_Identity=26, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24639252, Length=400, Percent_Identity=26, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24585130, Length=410, Percent_Identity=24.1463414634146, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 42714; Mature: 42583

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGMVIVGAGQAGLQTAESLRSGGYTGSIVLLGDEPCAPYHRPSLSKGFLLGEVSDTQLY
CCCEEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEE
IRAPGALNRKGIQWHASARVMRIEHATRTLHLDDGSTLGYTGLCLATGARARRLEVPGAG
EECCCCCCCCCEEEECCEEEEEEEECEEEEECCCCCCCCCCCEEEECCCCCEEEECCCCC
LGHVCMLRGMADTRALAAILPHTSRVVVIGGGFIGLEFASIARRLGKQVVVLEAADRLMA
CCHHHHHCCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHCCCEEEEEHHHHHHHH
RVVSPQLADFFLRLHRDNGATIELGSNVSALSGNRGVVTAVHTADGRVFPADLVVVGIGV
HHHCHHHHHHHHHEECCCCCEEEECCCEEEECCCCCEEEEEEECCCEEECCEEEEEEEEE
IPNGELAQQAGLACDRGALIVDACARSSSPGIVGAGDCTVRQRAGSGLLRLESVQNAIEQ
CCCCHHHHHHCCCCCCCCEEEEECCCCCCCCEEECCCCEEEHHCCCCCEEHHHHHHHHHH
AKSAAASLLGEHRPYPALPWFWSEQYEVRLQMAGFAAGHTQAVVRGDLATSTFSLFYYVD
HHHHHHHHHHCCCCCCCCCCEECCCEEEEEEEEEECCCCCEEEEECCCCCCEEEEEEEEC
GELCAVDSINRPQDHLAARKLLELGRSPTPQQAADPNFALTSLIPA
CCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHCCCC
>Mature Secondary Structure 
SGMVIVGAGQAGLQTAESLRSGGYTGSIVLLGDEPCAPYHRPSLSKGFLLGEVSDTQLY
CCEEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEE
IRAPGALNRKGIQWHASARVMRIEHATRTLHLDDGSTLGYTGLCLATGARARRLEVPGAG
EECCCCCCCCCEEEECCEEEEEEEECEEEEECCCCCCCCCCCEEEECCCCCEEEECCCCC
LGHVCMLRGMADTRALAAILPHTSRVVVIGGGFIGLEFASIARRLGKQVVVLEAADRLMA
CCHHHHHCCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHCCCEEEEEHHHHHHHH
RVVSPQLADFFLRLHRDNGATIELGSNVSALSGNRGVVTAVHTADGRVFPADLVVVGIGV
HHHCHHHHHHHHHEECCCCCEEEECCCEEEECCCCCEEEEEEECCCEEECCEEEEEEEEE
IPNGELAQQAGLACDRGALIVDACARSSSPGIVGAGDCTVRQRAGSGLLRLESVQNAIEQ
CCCCHHHHHHCCCCCCCCEEEEECCCCCCCCEEECCCCEEEHHCCCCCEEHHHHHHHHHH
AKSAAASLLGEHRPYPALPWFWSEQYEVRLQMAGFAAGHTQAVVRGDLATSTFSLFYYVD
HHHHHHHHHHCCCCCCCCCCEECCCEEEEEEEEEECCCCCEEEEECCCCCCEEEEEEEEC
GELCAVDSINRPQDHLAARKLLELGRSPTPQQAADPNFALTSLIPA
CCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]