Click here to switch to the map view.

The map label for this gene is otsB [H]

Identifier: 78044698

GI number: 78044698

Start: 585780

End: 586526

Strand: Direct

Name: otsB [H]

Synonym: CHY_0671

Alternate gene names: 78044698

Gene position: 585780-586526 (Clockwise)

Preceding gene: 78044465

Following gene: 78043188

Centisome position: 24.39

GC content: 43.37

Gene sequence:

>747_bases
TTGACTAAGCTTTCTGGCCGAAAAATACTGCTCATGACCGATTTTGACGGAACGATAGCGGCTTACAGAAAAGACCCGCG
CAAGGTAAGTTTAGCCAAAGATATGGTGGCAATCCTGCAGAAATTTACCCGGCAGCCAAATCTTAAATTAGCGGTGGTTT
CCGGACGGGGGCTTCAGGATTTAGAAAAAATGGTAAATATCCGGGGAATTATTTTAGCCGGCTGTTTTGGAGGAGTATTT
CGGGATGAACAGGGAAAAGTTCATACCTGGGAAAAGGCCCCCGACTATTTTGGACCGGTGGAGGAGTTAGCGGAATTTTT
TTCCCGTTCTCCCGTTTTTAAAGGAGTTTATATTGAAAAAAAAGAAATTGCCTTAACTTTACATTACAAAGATTTGGGAG
TAAAGAAACGGCGGGAGGTTTTAAAGGTAATTGAAGAAGCCCGGGAAAAAAACCCTATCTTTAACTTCCACGTGGGAGAT
AAAGGAACCGAAATTATCCCGCAGGGATTGGGCAAAGGATGGTTTATTCAGGAAATGTTAAAAAAATACCCGGACTTTTA
TCCGGTGTTTTTAGGGAATGACTGGGTAGACCTTGAGGGAATTGAAGTTTTAAGGGGGCAGGGTTTAGCATTTTACGTAG
GGGATACCCCGCCCCCGGGAAGTCACGGGCTTTCGGGGTTAAAAGAGGTGAAAAAGCTTTTTAAGAAGCTTTTGGCAGTA
AACCAAGCCGCTTTAAATGCATCTTAA

Upstream 100 bases:

>100_bases
GAATGGTGGCTGGAAAATTTCCTGGGAAAAGAACTACGGTTGATGGATACAGGTTTTGGCAAATACTACCGCAAACGGGG
TGAAAAGAAAGTTGGAGCTA

Downstream 100 bases:

>100_bases
TGCCGATGATTTCCCACATCCGTACCCAGGGGTCAATTTTTTGAAACTTTAAAGGATAAAAAATTGAATTATCGGCTATT
TCTTCGATGGTATATCCCTT

Product: trehalose-phosphatase

Products: NA

Alternate protein names: TPP; Osmoregulatory trehalose synthesis protein B; Trehalose 6-phosphate phosphatase; Trehalose-phosphatase [H]

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MTKLSGRKILLMTDFDGTIAAYRKDPRKVSLAKDMVAILQKFTRQPNLKLAVVSGRGLQDLEKMVNIRGIILAGCFGGVF
RDEQGKVHTWEKAPDYFGPVEELAEFFSRSPVFKGVYIEKKEIALTLHYKDLGVKKRREVLKVIEEAREKNPIFNFHVGD
KGTEIIPQGLGKGWFIQEMLKKYPDFYPVFLGNDWVDLEGIEVLRGQGLAFYVGDTPPPGSHGLSGLKEVKKLFKKLLAV
NQAALNAS

Sequences:

>Translated_248_residues
MTKLSGRKILLMTDFDGTIAAYRKDPRKVSLAKDMVAILQKFTRQPNLKLAVVSGRGLQDLEKMVNIRGIILAGCFGGVF
RDEQGKVHTWEKAPDYFGPVEELAEFFSRSPVFKGVYIEKKEIALTLHYKDLGVKKRREVLKVIEEAREKNPIFNFHVGD
KGTEIIPQGLGKGWFIQEMLKKYPDFYPVFLGNDWVDLEGIEVLRGQGLAFYVGDTPPPGSHGLSGLKEVKKLFKKLLAV
NQAALNAS
>Mature_247_residues
TKLSGRKILLMTDFDGTIAAYRKDPRKVSLAKDMVAILQKFTRQPNLKLAVVSGRGLQDLEKMVNIRGIILAGCFGGVFR
DEQGKVHTWEKAPDYFGPVEELAEFFSRSPVFKGVYIEKKEIALTLHYKDLGVKKRREVLKVIEEAREKNPIFNFHVGDK
GTEIIPQGLGKGWFIQEMLKKYPDFYPVFLGNDWVDLEGIEVLRGQGLAFYVGDTPPPGSHGLSGLKEVKKLFKKLLAVN
QAALNAS

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788207, Length=220, Percent_Identity=26.8181818181818, Blast_Score=73, Evalue=2e-14,
Organism=Drosophila melanogaster, GI221473368, Length=229, Percent_Identity=30.5676855895196, Blast_Score=103, Evalue=9e-23,
Organism=Drosophila melanogaster, GI20129307, Length=229, Percent_Identity=30.5676855895196, Blast_Score=103, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24582490, Length=229, Percent_Identity=30.5676855895196, Blast_Score=103, Evalue=9e-23,
Organism=Drosophila melanogaster, GI19920676, Length=247, Percent_Identity=25.5060728744939, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 27870; Mature: 27739

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLSGRKILLMTDFDGTIAAYRKDPRKVSLAKDMVAILQKFTRQPNLKLAVVSGRGLQD
CCCCCCCEEEEEECCCCCEEHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHH
LEKMVNIRGIILAGCFGGVFRDEQGKVHTWEKAPDYFGPVEELAEFFSRSPVFKGVYIEK
HHHHHHHHHHEEEHHHCCCEECCCCCEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEEEE
KEIALTLHYKDLGVKKRREVLKVIEEAREKNPIFNFHVGDKGTEIIPQGLGKGWFIQEML
EEEEEEEEEHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHCCCCHHHHHHH
KKYPDFYPVFLGNDWVDLEGIEVLRGQGLAFYVGDTPPPGSHGLSGLKEVKKLFKKLLAV
HHCCCEEEEEECCCEECCCCHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
NQAALNAS
HHHHCCCC
>Mature Secondary Structure 
TKLSGRKILLMTDFDGTIAAYRKDPRKVSLAKDMVAILQKFTRQPNLKLAVVSGRGLQD
CCCCCCEEEEEECCCCCEEHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHH
LEKMVNIRGIILAGCFGGVFRDEQGKVHTWEKAPDYFGPVEELAEFFSRSPVFKGVYIEK
HHHHHHHHHHEEEHHHCCCEECCCCCEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEEEE
KEIALTLHYKDLGVKKRREVLKVIEEAREKNPIFNFHVGDKGTEIIPQGLGKGWFIQEML
EEEEEEEEEHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHCCCCHHHHHHH
KKYPDFYPVFLGNDWVDLEGIEVLRGQGLAFYVGDTPPPGSHGLSGLKEVKKLFKKLLAV
HHCCCEEEEEECCCEECCCCHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
NQAALNAS
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8045430; 9097040; 9278503 [H]