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The map label for this gene is otsA [H]

Identifier: 78044465

GI number: 78044465

Start: 584354

End: 585787

Strand: Direct

Name: otsA [H]

Synonym: CHY_0670

Alternate gene names: 78044465

Gene position: 584354-585787 (Clockwise)

Preceding gene: 78044038

Following gene: 78044698

Centisome position: 24.33

GC content: 43.79

Gene sequence:

>1434_bases
ATGAGAGGTAATCTTTTGGTGGTATCTAACCGGGGACCCTTTACCATTGAAGAAAATAACGGTAGTTTAAAATACCGTCC
GGCGGTAAGTGGACTTGTATCGGCGGTCCTTCCCGCCGTAAAAAAAATCGGAGGAACCTGGTTAGCCTGGGCAGGGAGAA
TTGCGGAGAAGCCTGCGGAAGTTCAGGCGGTAATTAAAGACGAGTTTCAATTTGTGGAAATCCTCCTATCCAGGGAAGAA
GTAGAGGGGTATTATGAAGGTTATGCCAACGGAGTGCTCTGGCCGCTCTGCCACCTGATGCCGGAAAAAGTTAGGATGTA
CGAAGAAAATTACCGAATTTACCGGGAAGTTAACCAGAAATTTGCCCAAAAGGCTGCTACCTTATTAAGAACCGGGACGC
TTTTATGGATTCATGACTACCACTTAGCTTTAATGCCCCACTTTATTCGCCAAAAAAATCCCTGGCAGCGAATTGCCTTT
TTCTGGCATATACCCTTTCCGCCGGTAGAACTGTTTACCATTCAGCCCTGGGCGGAAGAAATTTTACGCGGCCTTTTAGG
TGCGGATATCATTGGTTTTCATATTGATGACTACCGGGAAAACTTTTTACGGGCGGTGGAGCGGGTATTAAAGCTTCCGG
TAAATTACGAGTGGGGTACCGTTTATTATAATGGACGAGAAGTCCTGGTCAAGGCGGTGCCGATTGGTATTGAAACTAAA
AACTTCCAAAAGGAAGTGCCTGAAATTACTGAAATTGAGCAAGAAATCGCAGGATTGAAAATCTTTCTGGGAGTCGAAAG
ATTGGATTATACCAAAGGTTTAAAAGAAAAAATCCAGGGGTTTGCCCGTTTTTTGGAGAAAAATCCCGAGTATCGGGGTA
AAGTTAAGCTTTTACAGGTGGCGGTACCGACCCGGGAAAATATTGACCAATACCAAACTTATGCCCGGGAGGTTTTGGCC
GAAGCCGCTAAGGTAAATGAACGTTTTGGCGATAACCACTGGCAGCCCATAAAGATTTTAAAGCGAAATTATAGCCAGGA
AGAACTTATCTATTTATACCGCATTGCCGATGCGCTTGTTATTACTTCCCTGGAAGATGGGCTTAACTTGGTGGCTAAAG
AATATATTGCTTCCAGAAAAGAGCCGGGAGTGCTTATTTTAAGTAAACGGACGGGAGTTGCCCGGCAACTTAATGCTGCT
CTTTTGGTCAATCCCTACTCGCCGGAAGAAATTGCTCTAAAAATGAAAGAAGCCATAGAAATGCCGGATACCCTCAAGAA
AAAGAGTTTTTCTTTATTAAAGGAACAGGTTATCTTAAAAAACAATGAATGGTGGCTGGAAAATTTCCTGGGAAAAGAAC
TACGGTTGATGGATACAGGTTTTGGCAAATACTACCGCAAACGGGGTGAAAAGAAAGTTGGAGCTATTGACTAA

Upstream 100 bases:

>100_bases
TTGATTTAGAGTTTAGCCGGAGCCGGGGTAAAACTTACTGTAACGTTGTGAAAAGGGAGGGAGAGGTGAAAGTCTTGATT
GAACCGTAGGAGGTGGATAG

Downstream 100 bases:

>100_bases
GCTTTCTGGCCGAAAAATACTGCTCATGACCGATTTTGACGGAACGATAGCGGCTTACAGAAAAGACCCGCGCAAGGTAA
GTTTAGCCAAAGATATGGTG

Product: alpha, alpha-trehalose-phosphate synthase

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MRGNLLVVSNRGPFTIEENNGSLKYRPAVSGLVSAVLPAVKKIGGTWLAWAGRIAEKPAEVQAVIKDEFQFVEILLSREE
VEGYYEGYANGVLWPLCHLMPEKVRMYEENYRIYREVNQKFAQKAATLLRTGTLLWIHDYHLALMPHFIRQKNPWQRIAF
FWHIPFPPVELFTIQPWAEEILRGLLGADIIGFHIDDYRENFLRAVERVLKLPVNYEWGTVYYNGREVLVKAVPIGIETK
NFQKEVPEITEIEQEIAGLKIFLGVERLDYTKGLKEKIQGFARFLEKNPEYRGKVKLLQVAVPTRENIDQYQTYAREVLA
EAAKVNERFGDNHWQPIKILKRNYSQEELIYLYRIADALVITSLEDGLNLVAKEYIASRKEPGVLILSKRTGVARQLNAA
LLVNPYSPEEIALKMKEAIEMPDTLKKKSFSLLKEQVILKNNEWWLENFLGKELRLMDTGFGKYYRKRGEKKVGAID

Sequences:

>Translated_477_residues
MRGNLLVVSNRGPFTIEENNGSLKYRPAVSGLVSAVLPAVKKIGGTWLAWAGRIAEKPAEVQAVIKDEFQFVEILLSREE
VEGYYEGYANGVLWPLCHLMPEKVRMYEENYRIYREVNQKFAQKAATLLRTGTLLWIHDYHLALMPHFIRQKNPWQRIAF
FWHIPFPPVELFTIQPWAEEILRGLLGADIIGFHIDDYRENFLRAVERVLKLPVNYEWGTVYYNGREVLVKAVPIGIETK
NFQKEVPEITEIEQEIAGLKIFLGVERLDYTKGLKEKIQGFARFLEKNPEYRGKVKLLQVAVPTRENIDQYQTYAREVLA
EAAKVNERFGDNHWQPIKILKRNYSQEELIYLYRIADALVITSLEDGLNLVAKEYIASRKEPGVLILSKRTGVARQLNAA
LLVNPYSPEEIALKMKEAIEMPDTLKKKSFSLLKEQVILKNNEWWLENFLGKELRLMDTGFGKYYRKRGEKKVGAID
>Mature_477_residues
MRGNLLVVSNRGPFTIEENNGSLKYRPAVSGLVSAVLPAVKKIGGTWLAWAGRIAEKPAEVQAVIKDEFQFVEILLSREE
VEGYYEGYANGVLWPLCHLMPEKVRMYEENYRIYREVNQKFAQKAATLLRTGTLLWIHDYHLALMPHFIRQKNPWQRIAF
FWHIPFPPVELFTIQPWAEEILRGLLGADIIGFHIDDYRENFLRAVERVLKLPVNYEWGTVYYNGREVLVKAVPIGIETK
NFQKEVPEITEIEQEIAGLKIFLGVERLDYTKGLKEKIQGFARFLEKNPEYRGKVKLLQVAVPTRENIDQYQTYAREVLA
EAAKVNERFGDNHWQPIKILKRNYSQEELIYLYRIADALVITSLEDGLNLVAKEYIASRKEPGVLILSKRTGVARQLNAA
LLVNPYSPEEIALKMKEAIEMPDTLKKKSFSLLKEQVILKNNEWWLENFLGKELRLMDTGFGKYYRKRGEKKVGAID

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=454, Percent_Identity=31.057268722467, Blast_Score=251, Evalue=8e-68,
Organism=Caenorhabditis elegans, GI115533560, Length=363, Percent_Identity=32.2314049586777, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI115533558, Length=363, Percent_Identity=32.2314049586777, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI71987012, Length=417, Percent_Identity=28.7769784172662, Blast_Score=173, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6319602, Length=465, Percent_Identity=37.8494623655914, Blast_Score=316, Evalue=4e-87,
Organism=Saccharomyces cerevisiae, GI6323537, Length=452, Percent_Identity=27.212389380531, Blast_Score=198, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6323917, Length=450, Percent_Identity=27.3333333333333, Blast_Score=186, Evalue=8e-48,
Organism=Saccharomyces cerevisiae, GI6320279, Length=366, Percent_Identity=30.6010928961749, Blast_Score=162, Evalue=1e-40,
Organism=Drosophila melanogaster, GI19920676, Length=482, Percent_Identity=35.6846473029046, Blast_Score=303, Evalue=1e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 55263; Mature: 55263

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGNLLVVSNRGPFTIEENNGSLKYRPAVSGLVSAVLPAVKKIGGTWLAWAGRIAEKPAE
CCCCEEEEECCCCEEEECCCCCEEECHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHH
VQAVIKDEFQFVEILLSREEVEGYYEGYANGVLWPLCHLMPEKVRMYEENYRIYREVNQK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
FAQKAATLLRTGTLLWIHDYHLALMPHFIRQKNPWQRIAFFWHIPFPPVELFTIQPWAEE
HHHHHHHHHHCCCEEEEECHHHHHHHHHHHCCCCHHEEEEEEECCCCCEEEEEECHHHHH
ILRGLLGADIIGFHIDDYRENFLRAVERVLKLPVNYEWGTVYYNGREVLVKAVPIGIETK
HHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEECCCCCCC
NFQKEVPEITEIEQEIAGLKIFLGVERLDYTKGLKEKIQGFARFLEKNPEYRGKVKLLQV
CHHHHCCHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
AVPTRENIDQYQTYAREVLAEAAKVNERFGDNHWQPIKILKRNYSQEELIYLYRIADALV
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHH
ITSLEDGLNLVAKEYIASRKEPGVLILSKRTGVARQLNAALLVNPYSPEEIALKMKEAIE
HHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHCCCEEEECCCCCHHHHHHHHHHHC
MPDTLKKKSFSLLKEQVILKNNEWWLENFLGKELRLMDTGFGKYYRKRGEKKVGAID
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHEEHHCCHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRGNLLVVSNRGPFTIEENNGSLKYRPAVSGLVSAVLPAVKKIGGTWLAWAGRIAEKPAE
CCCCEEEEECCCCEEEECCCCCEEECHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHH
VQAVIKDEFQFVEILLSREEVEGYYEGYANGVLWPLCHLMPEKVRMYEENYRIYREVNQK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
FAQKAATLLRTGTLLWIHDYHLALMPHFIRQKNPWQRIAFFWHIPFPPVELFTIQPWAEE
HHHHHHHHHHCCCEEEEECHHHHHHHHHHHCCCCHHEEEEEEECCCCCEEEEEECHHHHH
ILRGLLGADIIGFHIDDYRENFLRAVERVLKLPVNYEWGTVYYNGREVLVKAVPIGIETK
HHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEECCCCCCC
NFQKEVPEITEIEQEIAGLKIFLGVERLDYTKGLKEKIQGFARFLEKNPEYRGKVKLLQV
CHHHHCCHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
AVPTRENIDQYQTYAREVLAEAAKVNERFGDNHWQPIKILKRNYSQEELIYLYRIADALV
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHH
ITSLEDGLNLVAKEYIASRKEPGVLILSKRTGVARQLNAALLVNPYSPEEIALKMKEAIE
HHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHCCCEEEECCCCCHHHHHHHHHHHC
MPDTLKKKSFSLLKEQVILKNNEWWLENFLGKELRLMDTGFGKYYRKRGEKKVGAID
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHEEHHCCHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA