Click here to switch to the map view.

The map label for this gene is trmE

Identifier: 78044593

GI number: 78044593

Start: 2359

End: 3744

Strand: Direct

Name: trmE

Synonym: CHY_0006

Alternate gene names: 78044593

Gene position: 2359-3744 (Clockwise)

Preceding gene: 78045166

Following gene: 78043339

Centisome position: 0.1

GC content: 40.55

Gene sequence:

>1386_bases
ATGATGGAAGATACCATCGCTGCCATTTCCACCCCTTTGGGGGAAGGAGGCATTGGAATCGTAAGGGTTAGCGGTCCGGG
CGCGATTGAAGCTGTAAAAAATGTTTTTATACCTCGACAAAGCAAAGATTTGTCTAAAGTTCCAAGCTTTACTCTACATT
ATGGAAAAATCGTTGACCCTGCTGACGGCAAAATCGTTGATGAGGTTTTGGTATCGGTTATGCGGGCCCCTAAAAGCTAT
ACCGGTGAAGACGTGGTAGAAATAAACTGTCACGGCGGTATTGTGGCGGTGGAAAAAGTTTTAGAACTTATTTTAAAACA
AGGAATACGTTTAGCCGAACCCGGTGAATTTACCAAAAGGGCTTTTTTAAATGGACGCATCGATTTATCCCAGGCCGAAG
CGGTTATAGATATTATCCGGGCCAAGACCGAAGCAAGTTTGAAGCTTGCGGGACGACAACTTTCTGGCGAACTTCGGGAA
AAAATAAATGCTGTACGGCAAAAAATAATCAACATTTTGGCTTTCATTGAAGTAAGCATTGACTATCCGGAATACGAGTT
TGATGAAGTAACTCCCGAAACTGCCCTTAAAAACATCGATGAAATAATAAACGATGTCCGAAGACTTCTTTCTTCCTATG
AACGGGGACGGATTTTAAGAGAAGGTATTACCGCCGTTATAGCCGGAAAACCCAATGTGGGTAAATCGTCTTTATTAAAT
GCTTTATTACGGAAAAAAAGGGCAATAGTTACCGATATACCCGGCACTACCCGGGACGTGATTGAAGATTATTTAAATTT
AAAAGGCATTCCGGTTAAAATCGTCGATACCGCGGGTATCCGGGAAACGGAAGATTTAGTGGAAAAACTTGGAGTAGAAA
AGACCCGGGAGTATTTAAATCAGGCGGATGTGACGTTATTTGTTGTCGACGTTAGTATTGGGATTGATGAAGATGATGAG
AAGATTTTAAGTTTAATTAATAAAGATAAAAGCTTGTTGGTCATCAATAAAATTGACTTGCTGCAGGGTAAGGTAAATTT
TGAGCAATATGCAGTAAAAACAGGTATTAAAAATTTCGTTCCTTTTTCAGCCAGGAACTTTGAAGGTCTGGAAATTTTGG
AAAATAAGCTTTACGAAATTCTCATTCCCGAGCAGGAAGGAGAAGGTGAATCCGCTTTAATTTCCAACCTTCGCCATAAA
AATTACCTGGAAAAAGCTTTAAATTCGTTATTAAGTGCCAAGGAAAGTATAGCTTCTGGCGAGCCGGTAGATCTTGTGGC
CATTGATTTAAACGAGGCCTTAAGGGAATTAGGGGCAATTACCGGCGATGCTCTCGGTGATGAGATCATAAACGAAATAT
TTTCCCAATTTTGTGTTGGAAAGTAG

Upstream 100 bases:

>100_bases
GTAATAATAGCACCTAAAAGATAGGCAGGTTTAACACCTGCCTTTTTTCATCGATTAAATATTGTTATAATTTTTTAAAA
AGCTTTTAGGAGTGAAGGAA

Downstream 100 bases:

>100_bases
GGGTGTGAAATGGAATATCATGCTGGCGATTATGATGTTATTGTAGTGGGAGCCGGTCATGCTGGGGTGGAAGCGGCCCT
GGCCTCTGCCCGTATGGGAC

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MMEDTIAAISTPLGEGGIGIVRVSGPGAIEAVKNVFIPRQSKDLSKVPSFTLHYGKIVDPADGKIVDEVLVSVMRAPKSY
TGEDVVEINCHGGIVAVEKVLELILKQGIRLAEPGEFTKRAFLNGRIDLSQAEAVIDIIRAKTEASLKLAGRQLSGELRE
KINAVRQKIINILAFIEVSIDYPEYEFDEVTPETALKNIDEIINDVRRLLSSYERGRILREGITAVIAGKPNVGKSSLLN
ALLRKKRAIVTDIPGTTRDVIEDYLNLKGIPVKIVDTAGIRETEDLVEKLGVEKTREYLNQADVTLFVVDVSIGIDEDDE
KILSLINKDKSLLVINKIDLLQGKVNFEQYAVKTGIKNFVPFSARNFEGLEILENKLYEILIPEQEGEGESALISNLRHK
NYLEKALNSLLSAKESIASGEPVDLVAIDLNEALRELGAITGDALGDEIINEIFSQFCVGK

Sequences:

>Translated_461_residues
MMEDTIAAISTPLGEGGIGIVRVSGPGAIEAVKNVFIPRQSKDLSKVPSFTLHYGKIVDPADGKIVDEVLVSVMRAPKSY
TGEDVVEINCHGGIVAVEKVLELILKQGIRLAEPGEFTKRAFLNGRIDLSQAEAVIDIIRAKTEASLKLAGRQLSGELRE
KINAVRQKIINILAFIEVSIDYPEYEFDEVTPETALKNIDEIINDVRRLLSSYERGRILREGITAVIAGKPNVGKSSLLN
ALLRKKRAIVTDIPGTTRDVIEDYLNLKGIPVKIVDTAGIRETEDLVEKLGVEKTREYLNQADVTLFVVDVSIGIDEDDE
KILSLINKDKSLLVINKIDLLQGKVNFEQYAVKTGIKNFVPFSARNFEGLEILENKLYEILIPEQEGEGESALISNLRHK
NYLEKALNSLLSAKESIASGEPVDLVAIDLNEALRELGAITGDALGDEIINEIFSQFCVGK
>Mature_461_residues
MMEDTIAAISTPLGEGGIGIVRVSGPGAIEAVKNVFIPRQSKDLSKVPSFTLHYGKIVDPADGKIVDEVLVSVMRAPKSY
TGEDVVEINCHGGIVAVEKVLELILKQGIRLAEPGEFTKRAFLNGRIDLSQAEAVIDIIRAKTEASLKLAGRQLSGELRE
KINAVRQKIINILAFIEVSIDYPEYEFDEVTPETALKNIDEIINDVRRLLSSYERGRILREGITAVIAGKPNVGKSSLLN
ALLRKKRAIVTDIPGTTRDVIEDYLNLKGIPVKIVDTAGIRETEDLVEKLGVEKTREYLNQADVTLFVVDVSIGIDEDDE
KILSLINKDKSLLVINKIDLLQGKVNFEQYAVKTGIKNFVPFSARNFEGLEILENKLYEILIPEQEGEGESALISNLRHK
NYLEKALNSLLSAKESIASGEPVDLVAIDLNEALRELGAITGDALGDEIINEIFSQFCVGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=474, Percent_Identity=35.4430379746835, Blast_Score=239, Evalue=4e-63,
Organism=Homo sapiens, GI193082993, Length=463, Percent_Identity=35.6371490280778, Blast_Score=232, Evalue=7e-61,
Organism=Homo sapiens, GI193082988, Length=503, Percent_Identity=33.5984095427435, Blast_Score=231, Evalue=1e-60,
Organism=Escherichia coli, GI2367268, Length=464, Percent_Identity=36.4224137931034, Blast_Score=282, Evalue=4e-77,
Organism=Escherichia coli, GI87082120, Length=128, Percent_Identity=41.40625, Blast_Score=84, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17507259, Length=474, Percent_Identity=30.379746835443, Blast_Score=190, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6323665, Length=521, Percent_Identity=31.4779270633397, Blast_Score=196, Evalue=9e-51,
Organism=Drosophila melanogaster, GI45550813, Length=481, Percent_Identity=31.1850311850312, Blast_Score=214, Evalue=1e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_CARHZ (Q3AG56)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_358878.1
- HSSP:   Q9WYA4
- ProteinModelPortal:   Q3AG56
- SMR:   Q3AG56
- STRING:   Q3AG56
- GeneID:   3727714
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_0006
- NMPDR:   fig|246194.3.peg.104
- TIGR:   CHY_0006
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- BioCyc:   CHYD246194:CHY_0006-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 50657; Mature: 50657

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: BINDING 22-22 BINDING 87-87 BINDING 126-126 BINDING 461-461

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMEDTIAAISTPLGEGGIGIVRVSGPGAIEAVKNVFIPRQSKDLSKVPSFTLHYGKIVDP
CCCHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCHHHCCCCEEECCCEECC
ADGKIVDEVLVSVMRAPKSYTGEDVVEINCHGGIVAVEKVLELILKQGIRLAEPGEFTKR
CCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHH
AFLNGRIDLSQAEAVIDIIRAKTEASLKLAGRQLSGELREKINAVRQKIINILAFIEVSI
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
DYPEYEFDEVTPETALKNIDEIINDVRRLLSSYERGRILREGITAVIAGKPNVGKSSLLN
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCEEEEECCCCCCHHHHHH
ALLRKKRAIVTDIPGTTRDVIEDYLNLKGIPVKIVDTAGIRETEDLVEKLGVEKTREYLN
HHHHHCCCEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCHHHHHHHHC
QADVTLFVVDVSIGIDEDDEKILSLINKDKSLLVINKIDLLQGKVNFEQYAVKTGIKNFV
CCCEEEEEEEEEECCCCCHHHHHHHHCCCCCEEEEEHHHHHHCCCCHHHHHHHHHHHHCC
PFSARNFEGLEILENKLYEILIPEQEGEGESALISNLRHKNYLEKALNSLLSAKESIASG
CCCCCCCCHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EPVDLVAIDLNEALRELGAITGDALGDEIINEIFSQFCVGK
CCCEEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMEDTIAAISTPLGEGGIGIVRVSGPGAIEAVKNVFIPRQSKDLSKVPSFTLHYGKIVDP
CCCHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCHHHCCCCEEECCCEECC
ADGKIVDEVLVSVMRAPKSYTGEDVVEINCHGGIVAVEKVLELILKQGIRLAEPGEFTKR
CCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHH
AFLNGRIDLSQAEAVIDIIRAKTEASLKLAGRQLSGELREKINAVRQKIINILAFIEVSI
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
DYPEYEFDEVTPETALKNIDEIINDVRRLLSSYERGRILREGITAVIAGKPNVGKSSLLN
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCEEEEECCCCCCHHHHHH
ALLRKKRAIVTDIPGTTRDVIEDYLNLKGIPVKIVDTAGIRETEDLVEKLGVEKTREYLN
HHHHHCCCEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCHHHHHHHHC
QADVTLFVVDVSIGIDEDDEKILSLINKDKSLLVINKIDLLQGKVNFEQYAVKTGIKNFV
CCCEEEEEEEEEECCCCCHHHHHHHHCCCCCEEEEEHHHHHHCCCCHHHHHHHHHHHHCC
PFSARNFEGLEILENKLYEILIPEQEGEGESALISNLRHKNYLEKALNSLLSAKESIASG
CCCCCCCCHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EPVDLVAIDLNEALRELGAITGDALGDEIINEIFSQFCVGK
CCCEEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA