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The map label for this gene is gidA1

Identifier: 78043339

GI number: 78043339

Start: 3754

End: 5649

Strand: Direct

Name: gidA1

Synonym: CHY_0007

Alternate gene names: 78043339

Gene position: 3754-5649 (Clockwise)

Preceding gene: 78044593

Following gene: 78044839

Centisome position: 0.16

GC content: 43.99

Gene sequence:

>1896_bases
ATGGAATATCATGCTGGCGATTATGATGTTATTGTAGTGGGAGCCGGTCATGCTGGGGTGGAAGCGGCCCTGGCCTCTGC
CCGTATGGGACTTAAAACATTGCTTTTAACTTTGTCCCTGGATAACGTGGCTTTAATGCCCTGCAATCCTGCCGTTGGTG
GTCCCGCAAAAGGTGTGGTGGTTCGGGAAGTGGATGCTTTAGGCGGAGAAATGGGAATTAACACCGACAAAACTTATATT
CAAATACGGATGCTTAATACCGGAAAAGGACCGGCGGTTCGTACTTTGCGGGCGCAAGCGGATAAAAGACGTTACCAGGA
GGAAATGAAAAAAACCATTGAACGTCAGGAAAACCTTTACTTAAAACAGGCAGAAACCATCAAAATCTTGGTCGAAGGGG
AAAAAGTTCGGGGCGTCCTCACTCGAACCGGGGCTGTTTTTACTTCGCGAGCGGTTGTCGTTACCTCCGGGACTTATTTA
AATTCCCGGATTATTATTGGAGATGTCCACTATCCTTCCGGACCGGCGGGATTTCCTTACGCAAGTCTCCTGTCAAAAAG
TCTGGCCGATTTAGGATTTAAGCTTGGGCGTTTTAAAACCGGAACTCCTGCCCGGGTTGATAAAAGAACCGTTGATTTTT
CAAAAATGATTGAACAACCGGGCGATGACCGGCCTCTTTATTTTTCTTATATGAGCGAAGGAATAAAGCGACCTAATGTA
TCCTGCTGGCTTACCTACACCAATTTAGAAACTCATACGATTATCTTAGAAAATTTGCACCGTTCCCCTCTTTATTCGGG
GGAAATTAAAGGAGTTGGTCCCCGGTATTGTCCCTCAATTGAAGATAAGGTTGTACGTTTTTCCGATAAACCCAGACATC
AGGTGTTTTTAGAGCCGGAAGGTCTTGATACCTATGAAATGTATGTCCAGGGAATGTCTACAAGCCTTCCCGAAGACTTG
CAGATAAAAATGCTAAGAACCCTTCCCGGCCTTGAACGGGTGGAAATAATGCGCCCTGCTTATGCGATCGAGTATGACTA
TATTGATCCGACTCAGTTAAAGTTAACCTTAGAAGCAAAACACATTCAAGGACTTTTCTTTGCCGGGCAAATTAACGGGA
CTTCCGGTTACGAAGAAGCTGCCGGTCAGGGGATAGTCGCCGGAATTAATGCTGCTTTGTACGTTAAAGAAAAAGAACCG
TTTATCCTCAAAAGATCTGAAGCTTACATCGGGGTCATGATTGATGATCTTGTAACCAAGGGTGTTACTGATCCCTACCG
CCTTTTAACTTCCCGAGCGGAATATCGACTACTTTTGCGCCATGATAATGCTGATCTCCGCCTCACCGAAAAAGGTTATA
AAATTGGCCTTGTTACCGAAGAGCGGTGGCGAAAGTTCAATGAGCGGGTCGAGAAAATCAATAAATTGATGGAGTTTTTG
GAAGAGAACCAGGTAACTCCTACAAAAAGAAATCTTGAAATAATGGAGGAATACGGTACCTCTCCGCCCAAACACGGGAT
TTCCGGTAAAGAGTTTTTGAGGCGTCCGGAAATTAACATGGAAGCAATCGAAAAAATTTTTGCCATCGATGGGCAGTTTC
CCGATGATGTAAAAGAACAGGTGGAAATCTTAGTAAAATATGAAGGATACATCGAAAAACAACTGAAGGAAATCGAGCGC
TTTAATAAATACGAAGGTAAAAAATTACCTCCCGATTTTGATTACAGCAAGGTAAAAGGTTTATCTGCGGAGGCGGTCCA
AAAACTTAATGCTATAAAACCGGAAAATATTGGGCAGGCTTCGCGGGTTTCCGGGGTAACTCCAGCCGATATTTCGGTGC
TATTAATTTATTTAGAGAGTAGAAAAAGTGAGGATAACAATGGAAGCGAAGCTTAA

Upstream 100 bases:

>100_bases
GGCCTTAAGGGAATTAGGGGCAATTACCGGCGATGCTCTCGGTGATGAGATCATAAACGAAATATTTTCCCAATTTTGTG
TTGGAAAGTAGGGGTGTGAA

Downstream 100 bases:

>100_bases
AACTTTTTTTGCCGAGTTTGGACTGTCAATTGAAAAGGAAAAAATCCAGCTTTTTGAACGGTATTATCGCTTATTAATAT
CTGAAAATGAAAAATACAAC

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 631; Mature: 631

Protein sequence:

>631_residues
MEYHAGDYDVIVVGAGHAGVEAALASARMGLKTLLLTLSLDNVALMPCNPAVGGPAKGVVVREVDALGGEMGINTDKTYI
QIRMLNTGKGPAVRTLRAQADKRRYQEEMKKTIERQENLYLKQAETIKILVEGEKVRGVLTRTGAVFTSRAVVVTSGTYL
NSRIIIGDVHYPSGPAGFPYASLLSKSLADLGFKLGRFKTGTPARVDKRTVDFSKMIEQPGDDRPLYFSYMSEGIKRPNV
SCWLTYTNLETHTIILENLHRSPLYSGEIKGVGPRYCPSIEDKVVRFSDKPRHQVFLEPEGLDTYEMYVQGMSTSLPEDL
QIKMLRTLPGLERVEIMRPAYAIEYDYIDPTQLKLTLEAKHIQGLFFAGQINGTSGYEEAAGQGIVAGINAALYVKEKEP
FILKRSEAYIGVMIDDLVTKGVTDPYRLLTSRAEYRLLLRHDNADLRLTEKGYKIGLVTEERWRKFNERVEKINKLMEFL
EENQVTPTKRNLEIMEEYGTSPPKHGISGKEFLRRPEINMEAIEKIFAIDGQFPDDVKEQVEILVKYEGYIEKQLKEIER
FNKYEGKKLPPDFDYSKVKGLSAEAVQKLNAIKPENIGQASRVSGVTPADISVLLIYLESRKSEDNNGSEA

Sequences:

>Translated_631_residues
MEYHAGDYDVIVVGAGHAGVEAALASARMGLKTLLLTLSLDNVALMPCNPAVGGPAKGVVVREVDALGGEMGINTDKTYI
QIRMLNTGKGPAVRTLRAQADKRRYQEEMKKTIERQENLYLKQAETIKILVEGEKVRGVLTRTGAVFTSRAVVVTSGTYL
NSRIIIGDVHYPSGPAGFPYASLLSKSLADLGFKLGRFKTGTPARVDKRTVDFSKMIEQPGDDRPLYFSYMSEGIKRPNV
SCWLTYTNLETHTIILENLHRSPLYSGEIKGVGPRYCPSIEDKVVRFSDKPRHQVFLEPEGLDTYEMYVQGMSTSLPEDL
QIKMLRTLPGLERVEIMRPAYAIEYDYIDPTQLKLTLEAKHIQGLFFAGQINGTSGYEEAAGQGIVAGINAALYVKEKEP
FILKRSEAYIGVMIDDLVTKGVTDPYRLLTSRAEYRLLLRHDNADLRLTEKGYKIGLVTEERWRKFNERVEKINKLMEFL
EENQVTPTKRNLEIMEEYGTSPPKHGISGKEFLRRPEINMEAIEKIFAIDGQFPDDVKEQVEILVKYEGYIEKQLKEIER
FNKYEGKKLPPDFDYSKVKGLSAEAVQKLNAIKPENIGQASRVSGVTPADISVLLIYLESRKSEDNNGSEA
>Mature_631_residues
MEYHAGDYDVIVVGAGHAGVEAALASARMGLKTLLLTLSLDNVALMPCNPAVGGPAKGVVVREVDALGGEMGINTDKTYI
QIRMLNTGKGPAVRTLRAQADKRRYQEEMKKTIERQENLYLKQAETIKILVEGEKVRGVLTRTGAVFTSRAVVVTSGTYL
NSRIIIGDVHYPSGPAGFPYASLLSKSLADLGFKLGRFKTGTPARVDKRTVDFSKMIEQPGDDRPLYFSYMSEGIKRPNV
SCWLTYTNLETHTIILENLHRSPLYSGEIKGVGPRYCPSIEDKVVRFSDKPRHQVFLEPEGLDTYEMYVQGMSTSLPEDL
QIKMLRTLPGLERVEIMRPAYAIEYDYIDPTQLKLTLEAKHIQGLFFAGQINGTSGYEEAAGQGIVAGINAALYVKEKEP
FILKRSEAYIGVMIDDLVTKGVTDPYRLLTSRAEYRLLLRHDNADLRLTEKGYKIGLVTEERWRKFNERVEKINKLMEFL
EENQVTPTKRNLEIMEEYGTSPPKHGISGKEFLRRPEINMEAIEKIFAIDGQFPDDVKEQVEILVKYEGYIEKQLKEIER
FNKYEGKKLPPDFDYSKVKGLSAEAVQKLNAIKPENIGQASRVSGVTPADISVLLIYLESRKSEDNNGSEA

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=650, Percent_Identity=40.4615384615385, Blast_Score=445, Evalue=1e-125,
Organism=Homo sapiens, GI19882217, Length=675, Percent_Identity=38.962962962963, Blast_Score=431, Evalue=1e-120,
Organism=Homo sapiens, GI183227703, Length=690, Percent_Identity=38.1159420289855, Blast_Score=426, Evalue=1e-119,
Organism=Escherichia coli, GI2367273, Length=621, Percent_Identity=51.207729468599, Blast_Score=635, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=619, Percent_Identity=38.9337641357027, Blast_Score=451, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6321202, Length=617, Percent_Identity=41.3290113452188, Blast_Score=471, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24658174, Length=634, Percent_Identity=41.6403785488959, Blast_Score=449, Evalue=1e-126,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_CARHZ (Q3AG55)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_358879.1
- HSSP:   Q5SH33
- ProteinModelPortal:   Q3AG55
- SMR:   Q3AG55
- STRING:   Q3AG55
- GeneID:   3728528
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_0007
- NMPDR:   fig|246194.3.peg.105
- TIGR:   CHY_0007
- eggNOG:   COG0445
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- ProtClustDB:   PRK05192
- BioCyc:   CHYD246194:CHY_0007-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 70814; Mature: 70814

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYHAGDYDVIVVGAGHAGVEAALASARMGLKTLLLTLSLDNVALMPCNPAVGGPAKGVV
CCCCCCCEEEEEEECCCCCHHHHHHHHHHCHHHEEEEEEECCEEEEECCCCCCCCCCCEE
VREVDALGGEMGINTDKTYIQIRMLNTGKGPAVRTLRAQADKRRYQEEMKKTIERQENLY
EEEHHHCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEE
LKQAETIKILVEGEKVRGVLTRTGAVFTSRAVVVTSGTYLNSRIIIGDVHYPSGPAGFPY
EECCCEEEEEEECCHHCEEHHHCCCEEECCEEEEECCCEEECEEEEEEECCCCCCCCCCH
ASLLSKSLADLGFKLGRFKTGTPARVDKRTVDFSKMIEQPGDDRPLYFSYMSEGIKRPNV
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHCCCCCCCEEHHHHHHHCCCCCC
SCWLTYTNLETHTIILENLHRSPLYSGEIKGVGPRYCPSIEDKVVRFSDKPRHQVFLEPE
EEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHEECCCCCCCEEEECCC
GLDTYEMYVQGMSTSLPEDLQIKMLRTLPGLERVEIMRPAYAIEYDYIDPTQLKLTLEAK
CCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHCCEEEEEECCCCCCEEEEEEEEE
HIQGLFFAGQINGTSGYEEAAGQGIVAGINAALYVKEKEPFILKRSEAYIGVMIDDLVTK
EECEEEEEEEECCCCCHHHHCCCCEEEECCEEEEEECCCCEEEEECCCEEEEEHHHHHHC
GVTDPYRLLTSRAEYRLLLRHDNADLRLTEKGYKIGLVTEERWRKFNERVEKINKLMEFL
CCCCHHHHHHCCCCEEEEEEECCCCEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHH
EENQVTPTKRNLEIMEEYGTSPPKHGISGKEFLRRPEINMEAIEKIFAIDGQFPDDVKEQ
HHCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHH
VEILVKYEGYIEKQLKEIERFNKYEGKKLPPDFDYSKVKGLSAEAVQKLNAIKPENIGQA
HHEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCHHHHHHHHCCCCCCCCCH
SRVSGVTPADISVLLIYLESRKSEDNNGSEA
HHCCCCCCCCEEEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MEYHAGDYDVIVVGAGHAGVEAALASARMGLKTLLLTLSLDNVALMPCNPAVGGPAKGVV
CCCCCCCEEEEEEECCCCCHHHHHHHHHHCHHHEEEEEEECCEEEEECCCCCCCCCCCEE
VREVDALGGEMGINTDKTYIQIRMLNTGKGPAVRTLRAQADKRRYQEEMKKTIERQENLY
EEEHHHCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEE
LKQAETIKILVEGEKVRGVLTRTGAVFTSRAVVVTSGTYLNSRIIIGDVHYPSGPAGFPY
EECCCEEEEEEECCHHCEEHHHCCCEEECCEEEEECCCEEECEEEEEEECCCCCCCCCCH
ASLLSKSLADLGFKLGRFKTGTPARVDKRTVDFSKMIEQPGDDRPLYFSYMSEGIKRPNV
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHCCCCCCCEEHHHHHHHCCCCCC
SCWLTYTNLETHTIILENLHRSPLYSGEIKGVGPRYCPSIEDKVVRFSDKPRHQVFLEPE
EEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHEECCCCCCCEEEECCC
GLDTYEMYVQGMSTSLPEDLQIKMLRTLPGLERVEIMRPAYAIEYDYIDPTQLKLTLEAK
CCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHCCEEEEEECCCCCCEEEEEEEEE
HIQGLFFAGQINGTSGYEEAAGQGIVAGINAALYVKEKEPFILKRSEAYIGVMIDDLVTK
EECEEEEEEEECCCCCHHHHCCCCEEEECCEEEEEECCCCEEEEECCCEEEEEHHHHHHC
GVTDPYRLLTSRAEYRLLLRHDNADLRLTEKGYKIGLVTEERWRKFNERVEKINKLMEFL
CCCCHHHHHHCCCCEEEEEEECCCCEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHH
EENQVTPTKRNLEIMEEYGTSPPKHGISGKEFLRRPEINMEAIEKIFAIDGQFPDDVKEQ
HHCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHH
VEILVKYEGYIEKQLKEIERFNKYEGKKLPPDFDYSKVKGLSAEAVQKLNAIKPENIGQA
HHEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCHHHHHHHHCCCCCCCCCH
SRVSGVTPADISVLLIYLESRKSEDNNGSEA
HHCCCCCCCCEEEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA