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The map label for this gene is glmU

Identifier: 78044455

GI number: 78044455

Start: 160130

End: 161470

Strand: Direct

Name: glmU

Synonym: CHY_0192

Alternate gene names: 78044455

Gene position: 160130-161470 (Clockwise)

Preceding gene: 78043335

Following gene: 78044887

Centisome position: 6.67

GC content: 45.12

Gene sequence:

>1341_bases
GTGGAAGGAATTATCTTAGCGGCCGGTAAAGGCACGCGAATGAAATCGGATTTACCCAAAGTAGTACACGAAGTTGCCGA
AAAACCAATGGTATTGAGGGTGTATGAAGCTTTAGTTGGTGCCGGGGTTAAGCGGGTGGTCGCGGTGGTGGGCTACCGCA
AAGAAAAGGTGGAGGAAATTCTCCGCGGCCGGGCGGTAATTGCAGTTCAGGAAGAACAGTTAGGAACCGGGCATGCAGCT
TTGGTGGCTATGCCTTACGTTGAAGACGAGAATGTTATTATTGTCCCCGGAGATACACCCCTTTTAAAAGCTTCTACCCT
TCAAGCTTTAATCAAAAAACATCTTGAGACCGGGGCTTATGCCACGGTATTAACCTGTTTTTTAAGTAATCCCTATGGTT
ACGGCCGTATTGTCCGGGACGGATACGGGAAAATAATAAAAATAGTCGAGGAAAAGGATGCAACCCTGGAAGAAAAGCAA
ATAGCGGAGGTTAACACGGGAATTTACTGTTTTAATACAAAAATTTTAAAAGAAATCTTGCCGTTATTAAAAGCGGAAAA
TGCCCAAAAGGAGTATTACCTGACCGATGTTATTCCCCTTCTTTTAGAACGGGGTAAGGTAGTTGAAACGATTACTATAC
AGGATGAAACGGAAGTTTATGGGGTAAATGACCGGGTGCAGCTTGCTCGCTTGACCAAGGGAGTTTACCGGAGAAAAGCT
GAGGCGTTAATGCAGGAAGGGGTTACAATAATTGACCCGGAGACCGTGTATATTGGTGAAGAAGTAGTGGTGGGCAGTGA
TACGGTTATTTATCCCAATACTTACCTTGAAGGGAAAACGGTTATTGGGAGTGGGTGCCGCCTTGGACCCAATACCCGCA
TTACCGATAGCGTGATTGGCAACAATACCGAAATTACTTTTTCCGTAATTATCCAGGCCCGGGTTGGCGATGAGGTGAAC
GTGGGACCGTTTGCTTATCTTCGACCTGGGACCGAAATTGCTAACGGGGTAAAAATTGGAGATTTTGTGGAAATTAAAAA
ATCGTTCATTGGCGAAGGCTCCAAGGTTCCCCACCTGTCTTACATTGGGGATGCGGTAGTGGGTAAAGGAGTTAATATTG
GGGCCGGAACAATTACATGTAATTACGACGGGAAAAATAAGTGGGAAACGGTTATTGAAGATGGAGCGTTTATCGGAAGC
AATACCAATTTGGTGGCACCGATAAAGATTGGGAAAAATGCTGTAGTTGGGGCAGGCTCCACATTAACCGAAGATGTTCC
GGAAAAAGCTTTGGCTATAGCCCGTTCCCGCCAGGTGAATAAAGAAGATTATGTTAAATAA

Upstream 100 bases:

>100_bases
CTGGCTCTGGAAACAAGGGATTTATCTCACATTGAAAAAATTCTGGCGGTTTTAAGAGAAGCGGGTTATCAGATTCTACA
AAATTAAGGAGGGCTTTTTA

Downstream 100 bases:

>100_bases
TATTTATTTTTTCGTTAAAGCGGCAGGAAGTTTTCCTATTGTAGGAGAATAAAAAAACTGTCGAATTTTGCTCGGAACAT
GGATTAAAGTAAATGGAGGT

Product: UDP-N-acetylglucosamine pyrophosphorylase

Products: NA

Alternate protein names: UDP-N-acetylglucosamine pyrophosphorylase; N-acetylglucosamine-1-phosphate uridyltransferase; Glucosamine-1-phosphate N-acetyltransferase

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MEGIILAAGKGTRMKSDLPKVVHEVAEKPMVLRVYEALVGAGVKRVVAVVGYRKEKVEEILRGRAVIAVQEEQLGTGHAA
LVAMPYVEDENVIIVPGDTPLLKASTLQALIKKHLETGAYATVLTCFLSNPYGYGRIVRDGYGKIIKIVEEKDATLEEKQ
IAEVNTGIYCFNTKILKEILPLLKAENAQKEYYLTDVIPLLLERGKVVETITIQDETEVYGVNDRVQLARLTKGVYRRKA
EALMQEGVTIIDPETVYIGEEVVVGSDTVIYPNTYLEGKTVIGSGCRLGPNTRITDSVIGNNTEITFSVIIQARVGDEVN
VGPFAYLRPGTEIANGVKIGDFVEIKKSFIGEGSKVPHLSYIGDAVVGKGVNIGAGTITCNYDGKNKWETVIEDGAFIGS
NTNLVAPIKIGKNAVVGAGSTLTEDVPEKALAIARSRQVNKEDYVK

Sequences:

>Translated_446_residues
MEGIILAAGKGTRMKSDLPKVVHEVAEKPMVLRVYEALVGAGVKRVVAVVGYRKEKVEEILRGRAVIAVQEEQLGTGHAA
LVAMPYVEDENVIIVPGDTPLLKASTLQALIKKHLETGAYATVLTCFLSNPYGYGRIVRDGYGKIIKIVEEKDATLEEKQ
IAEVNTGIYCFNTKILKEILPLLKAENAQKEYYLTDVIPLLLERGKVVETITIQDETEVYGVNDRVQLARLTKGVYRRKA
EALMQEGVTIIDPETVYIGEEVVVGSDTVIYPNTYLEGKTVIGSGCRLGPNTRITDSVIGNNTEITFSVIIQARVGDEVN
VGPFAYLRPGTEIANGVKIGDFVEIKKSFIGEGSKVPHLSYIGDAVVGKGVNIGAGTITCNYDGKNKWETVIEDGAFIGS
NTNLVAPIKIGKNAVVGAGSTLTEDVPEKALAIARSRQVNKEDYVK
>Mature_446_residues
MEGIILAAGKGTRMKSDLPKVVHEVAEKPMVLRVYEALVGAGVKRVVAVVGYRKEKVEEILRGRAVIAVQEEQLGTGHAA
LVAMPYVEDENVIIVPGDTPLLKASTLQALIKKHLETGAYATVLTCFLSNPYGYGRIVRDGYGKIIKIVEEKDATLEEKQ
IAEVNTGIYCFNTKILKEILPLLKAENAQKEYYLTDVIPLLLERGKVVETITIQDETEVYGVNDRVQLARLTKGVYRRKA
EALMQEGVTIIDPETVYIGEEVVVGSDTVIYPNTYLEGKTVIGSGCRLGPNTRITDSVIGNNTEITFSVIIQARVGDEVN
VGPFAYLRPGTEIANGVKIGDFVEIKKSFIGEGSKVPHLSYIGDAVVGKGVNIGAGTITCNYDGKNKWETVIEDGAFIGS
NTNLVAPIKIGKNAVVGAGSTLTEDVPEKALAIARSRQVNKEDYVK

Specific function: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-GlcNAc. Responsible for the acetylation of Glc-N-1-P to give GlcNAc-1-P and for the uridyl transfer from UTP to GlcNAc-1-P which produces UDP-GlcNAc

COG id: COG1207

COG function: function code M; N-acetylglucosamine-1-phosphate uridyltransferase (contains nucleotidyltransferase and I-patch acetyltransferase domains)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transferase hexapeptide repeat family

Homologues:

Organism=Homo sapiens, GI11761621, Length=377, Percent_Identity=22.5464190981432, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI11761619, Length=333, Percent_Identity=22.5225225225225, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1790168, Length=447, Percent_Identity=44.7427293064877, Blast_Score=357, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI133931050, Length=390, Percent_Identity=27.6923076923077, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320148, Length=390, Percent_Identity=27.9487179487179, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21355443, Length=335, Percent_Identity=24.1791044776119, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24644084, Length=335, Percent_Identity=24.1791044776119, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLMU_CARHZ (Q3AFM0)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_359064.1
- ProteinModelPortal:   Q3AFM0
- SMR:   Q3AFM0
- STRING:   Q3AFM0
- GeneID:   3727574
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_0192
- NMPDR:   fig|246194.3.peg.1011
- TIGR:   CHY_0192
- eggNOG:   COG1207
- HOGENOM:   HBG688195
- OMA:   GSKVNHL
- BioCyc:   CHYD246194:CHY_0192-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01631
- InterPro:   IPR005882
- InterPro:   IPR018357
- InterPro:   IPR005835
- InterPro:   IPR011004
- TIGRFAMs:   TIGR01173

Pfam domain/function: PF00483 NTP_transferase; SSF51161 Trimer_LpxA_like

EC number: =2.7.7.23; =2.3.1.157

Molecular weight: Translated: 48381; Mature: 48381

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: ACT_SITE 358-358 BINDING 70-70 BINDING 135-135 BINDING 150-150 BINDING 165-165 BINDING 382-382 BINDING 400-400 BINDING 418-418 BINDING 435-435

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGIILAAGKGTRMKSDLPKVVHEVAEKPMVLRVYEALVGAGVKRVVAVVGYRKEKVEEI
CCCEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHCCCHHHHHHH
LRGRAVIAVQEEQLGTGHAALVAMPYVEDENVIIVPGDTPLLKASTLQALIKKHLETGAY
HCCCEEEEEEHHHCCCCCEEEEEECEECCCCEEEECCCCCCCHHHHHHHHHHHHHCCCHH
ATVLTCFLSNPYGYGRIVRDGYGKIIKIVEEKDATLEEKQIAEVNTGIYCFNTKILKEIL
HHHHHHHHCCCCCCCEEEECCCCCEEEEEECCCCCCCHHHHHHHCCCEEEECHHHHHHHH
PLLKAENAQKEYYLTDVIPLLLERGKVVETITIQDETEVYGVNDRVQLARLTKGVYRRKA
HHHHCCCCCCCEEHHHHHHHHHCCCCEEEEEEEECCCEEEECCCHHHHHHHHHHHHHHHH
EALMQEGVTIIDPETVYIGEEVVVGSDTVIYPNTYLEGKTVIGSGCRLGPNTRITDSVIG
HHHHHCCCEEECCCEEEECCEEEECCCEEECCCCEECCCEEECCCCCCCCCCCEEHHHCC
NNTEITFSVIIQARVGDEVNVGPFAYLRPGTEIANGVKIGDFVEIKKSFIGEGSKVPHLS
CCCEEEEEEEEEEECCCCCCCCCEEEECCCHHHHCCCCCCHHHHHHHHHCCCCCCCCCHH
YIGDAVVGKGVNIGAGTITCNYDGKNKWETVIEDGAFIGSNTNLVAPIKIGKNAVVGAGS
HHCHHHHCCCCEECCCEEEEECCCCCHHHHHHCCCCEECCCCCEEEEEEECCCEEEECCC
TLTEDVPEKALAIARSRQVNKEDYVK
CHHHHHHHHHHHHHHHCCCCHHHCCC
>Mature Secondary Structure
MEGIILAAGKGTRMKSDLPKVVHEVAEKPMVLRVYEALVGAGVKRVVAVVGYRKEKVEEI
CCCEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHCCCHHHHHHH
LRGRAVIAVQEEQLGTGHAALVAMPYVEDENVIIVPGDTPLLKASTLQALIKKHLETGAY
HCCCEEEEEEHHHCCCCCEEEEEECEECCCCEEEECCCCCCCHHHHHHHHHHHHHCCCHH
ATVLTCFLSNPYGYGRIVRDGYGKIIKIVEEKDATLEEKQIAEVNTGIYCFNTKILKEIL
HHHHHHHHCCCCCCCEEEECCCCCEEEEEECCCCCCCHHHHHHHCCCEEEECHHHHHHHH
PLLKAENAQKEYYLTDVIPLLLERGKVVETITIQDETEVYGVNDRVQLARLTKGVYRRKA
HHHHCCCCCCCEEHHHHHHHHHCCCCEEEEEEEECCCEEEECCCHHHHHHHHHHHHHHHH
EALMQEGVTIIDPETVYIGEEVVVGSDTVIYPNTYLEGKTVIGSGCRLGPNTRITDSVIG
HHHHHCCCEEECCCEEEECCEEEECCCEEECCCCEECCCEEECCCCCCCCCCCEEHHHCC
NNTEITFSVIIQARVGDEVNVGPFAYLRPGTEIANGVKIGDFVEIKKSFIGEGSKVPHLS
CCCEEEEEEEEEEECCCCCCCCCEEEECCCHHHHCCCCCCHHHHHHHHHCCCCCCCCCHH
YIGDAVVGKGVNIGAGTITCNYDGKNKWETVIEDGAFIGSNTNLVAPIKIGKNAVVGAGS
HHCHHHHCCCCEECCCEEEEECCCCCHHHHHHCCCCEECCCCCEEEEEEECCCEEEECCC
TLTEDVPEKALAIARSRQVNKEDYVK
CHHHHHHHHHHHHHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA