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The map label for this gene is napA [H]

Identifier: 78043983

GI number: 78043983

Start: 518216

End: 520843

Strand: Direct

Name: napA [H]

Synonym: CHY_0601

Alternate gene names: 78043983

Gene position: 518216-520843 (Clockwise)

Preceding gene: 78044793

Following gene: 78042641

Centisome position: 21.58

GC content: 47.18

Gene sequence:

>2628_bases
ATGGGTTTAAAGGCCGTCGGAAAAAGCCGCTGGATTGAAAATTTTAGCCAGGATATAGCTGAAGCTGCCAATCTTCCGGC
GGAAAAGTGGGTAAACAGCGTCTGCCCATATTGCGGTGCTGGTTGCTCCATCCTTTACGGGGTAAGGGAAGGAAAAGTGG
TAAGTGTTCGGGGTAATCCCGAGGCCTGGAACCGGGGTGGTGTTTGTATAAAAGGAGCAACCCTGGCGGATTTGTACCTT
AGCAATTCTCCCGGGGTCAAAGATCGGCTGAAAAAACCGCTTTTGCGGGACCCGGAGAAAAGGGGTAGTTTGGAGAATTT
CCGGGAAGTTTCCTGGGACGAAGCGTTAAATTATATGGTTAAGAAAGTAAAAGAATACACAAAGACAAAGTTTGGGGATA
AGCACCCCTTTGCCATGTACGGCAGTGGTCAGGTATCTACTGAATTTATGTACCTGCAGGCAAAATTCAATGTTATAGGC
ATGAATATTCATAACGACAACAACGGCCGGTTATGCCAGGCAACTAAAGCCAACGCTATGGTGATTTCCTTTGGTATCGA
TGGCCCGCCTATGAGCTTTGACGATGTTTTTGAGGCAGATAACCTGTTTATCTTTGGTTTTAACATAGCTGACACCTTAC
CGGGTTGGTTTGGCAAAATAGTGGAGGCTAAAGTCCAAAAAGGTGATAACTTAAAAATCGCCGTGATAAATCCCGTGAAG
ATTTCCGCCACCCAGATTTTAAATTATAAAGCTGGGGATTTGTATCTTCCGATTATTCCTACCACCGATGTGGCCTTTAT
TAACAGTATGGCCCATGTAATAATTTATGAACTTGAGGGGGTAAACCGGGATTATGCCGGTGATGTGAGCAGGTGGGCCG
ATGATGTAATAAGCGGCAAAGTCAAGCCTAAACATATCGACACCGAATTTATCGCCAAATACGTAAATTTCTATCGGGGT
GATTATCAAGTTTTAGCCCGGCTGGGTAAAGAAGGTCCTAAAATTTTCGAGGAAATTAAGCTTCCTTCCGGACTGGAAGG
GTTTAAAGAATATGCCAAATTTTTAGCCGCGTACAAACCGGAAGATGTAGCCAAAACTACTGGATTAACCCCGGAAGATA
TTCGCAAGGCAGCTCAAATGTTTGTCCGAAATAAAAATACCATGTCTATGTATCTGCAGGGCTTTGGCCAGCAGTCAAAT
GGGGTTTCCAAGCACTTGGCTCTGGTAACCCTGCACGCGGTAACCGGCCGGATTGGCAGAGCAGGAGCAGGGGTAATGCC
GACGGTAGGTCAGCCCAATGGACTTGGTCAAAGAGTTGGAGGTGCGGTGGTAGGCAGACTTCCGGGCAACCGTAATCACC
CGCTGGCTGCCCATCGGGCATCACTGGCCAAGGCTATGGCCCGGGGGAACCAGGAAATGGAAGCCAAAATTTTGGAACGG
CTTGAAGACCCGGTAACAAAGTATTCCCGACTTTCTCTGACCTCCGTTGATATCTTTAAGAACCTCAAAAATGGAAACAT
AAAAGGGATCTGGACGGTTTGCACTAACCCGATGGTGGCGTTTCCCAACCTCAACATGGTTCATGAGGCTTTACGGAAAG
CCGATTTAGTGGTGGTTCAAGATATCTATCCTACCGAAACCATGGCTTTTGCCGATGTGGTGCTCCCGGCAGCGGCTATT
TCCGGAGAAGCGTACGGGACATATTTAAATTCGGAGCGGCGAGTCCAGATATTAGAAAAAGCGGTGGAACCCCCCGGGGA
CGCGTTACCCGATGAAATTATTCACCTTGCTTTTGTTTACAAGTACCAGAAGGCTTTGGCCGAAGAAGGGCGTCAGGAAG
ACGCAGATTTTCTAAAGTTTTTATTAGAGCCCATAATTCAGGGTTACGAGCCATTATTTGAAAATGTCCAGGCTAATATC
CGGGAATTAAGGGAAGCTCATCAGGAAATTCAAAAACGACTGTTTAGCGAACTTGCGTATGTTTCTAAGGGAATACCAGG
CAATGATTTAAGCGGCTTATCCTATGAACGGTTAAGGACGGAAAAAGATGCTAAAGGGTATGTGGGCTTTAAGCTTCCCG
TTTTGGACAGCAGTCACAAAGGTCTTGAACGGCTTTACGATGAAAACTACGAAGCAATATACCAAAAACGTTTTGCTACT
CCGGACGGAAAACTTAGATGTTTCTTATTTGAGCAAGTTCATCCCGCAGAGGAATGGATTGATGCTGAATATCCGTTTAT
GCTCACTCTACCCCGGATTTATGAGCACTGGCATACCAGGACCCGGACGGGACGCTGCGCATTGCCCCACCGGCTGCGCC
CCGAAGCCTGGCTGTCCATAAATCCCAACGATGCGAAGAAACTTGGTATAAAGGACGGGGACTGGGTAGAAGTGGAAAGC
CGTCGGGGTAAGGTTACGGTGAAAGCCAAAATCGATACTGTAAAAACACCGCGGGAAGGGGTGGTTTGGATGCCCTGGGC
TTATGGCTTTTTAGGGAATTTGTTTACCGGCCAAAAAGAGGCGCCTCCCGGAAAATCCGCCGGAAATGTCTTAAGTACCG
ATGCTTACGACCCCGTTTCCAAACAGCCCGAACTAAAATTTGCCGCGGTGAAAATAAGAAAGGTGTGA

Upstream 100 bases:

>100_bases
ATTATTATAACAAACATAGTTAAAAAGGGGGTAGGTCAGCGTGGCTCGAAAATTTACACGGCGTAAATTTTTAAAGGGCG
CTGGTGCCGGGGCAGCGCTT

Downstream 100 bases:

>100_bases
TGACGGATGGCACGTTACGGAATGGTTATTGACCTGCGTAAATGCCGGGGGTGCCGCGGTTGTACCGCGGTTTGTGCCGT
GGAAAATAATGTTCCGGTAG

Product: nitrate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 875; Mature: 874

Protein sequence:

>875_residues
MGLKAVGKSRWIENFSQDIAEAANLPAEKWVNSVCPYCGAGCSILYGVREGKVVSVRGNPEAWNRGGVCIKGATLADLYL
SNSPGVKDRLKKPLLRDPEKRGSLENFREVSWDEALNYMVKKVKEYTKTKFGDKHPFAMYGSGQVSTEFMYLQAKFNVIG
MNIHNDNNGRLCQATKANAMVISFGIDGPPMSFDDVFEADNLFIFGFNIADTLPGWFGKIVEAKVQKGDNLKIAVINPVK
ISATQILNYKAGDLYLPIIPTTDVAFINSMAHVIIYELEGVNRDYAGDVSRWADDVISGKVKPKHIDTEFIAKYVNFYRG
DYQVLARLGKEGPKIFEEIKLPSGLEGFKEYAKFLAAYKPEDVAKTTGLTPEDIRKAAQMFVRNKNTMSMYLQGFGQQSN
GVSKHLALVTLHAVTGRIGRAGAGVMPTVGQPNGLGQRVGGAVVGRLPGNRNHPLAAHRASLAKAMARGNQEMEAKILER
LEDPVTKYSRLSLTSVDIFKNLKNGNIKGIWTVCTNPMVAFPNLNMVHEALRKADLVVVQDIYPTETMAFADVVLPAAAI
SGEAYGTYLNSERRVQILEKAVEPPGDALPDEIIHLAFVYKYQKALAEEGRQEDADFLKFLLEPIIQGYEPLFENVQANI
RELREAHQEIQKRLFSELAYVSKGIPGNDLSGLSYERLRTEKDAKGYVGFKLPVLDSSHKGLERLYDENYEAIYQKRFAT
PDGKLRCFLFEQVHPAEEWIDAEYPFMLTLPRIYEHWHTRTRTGRCALPHRLRPEAWLSINPNDAKKLGIKDGDWVEVES
RRGKVTVKAKIDTVKTPREGVVWMPWAYGFLGNLFTGQKEAPPGKSAGNVLSTDAYDPVSKQPELKFAAVKIRKV

Sequences:

>Translated_875_residues
MGLKAVGKSRWIENFSQDIAEAANLPAEKWVNSVCPYCGAGCSILYGVREGKVVSVRGNPEAWNRGGVCIKGATLADLYL
SNSPGVKDRLKKPLLRDPEKRGSLENFREVSWDEALNYMVKKVKEYTKTKFGDKHPFAMYGSGQVSTEFMYLQAKFNVIG
MNIHNDNNGRLCQATKANAMVISFGIDGPPMSFDDVFEADNLFIFGFNIADTLPGWFGKIVEAKVQKGDNLKIAVINPVK
ISATQILNYKAGDLYLPIIPTTDVAFINSMAHVIIYELEGVNRDYAGDVSRWADDVISGKVKPKHIDTEFIAKYVNFYRG
DYQVLARLGKEGPKIFEEIKLPSGLEGFKEYAKFLAAYKPEDVAKTTGLTPEDIRKAAQMFVRNKNTMSMYLQGFGQQSN
GVSKHLALVTLHAVTGRIGRAGAGVMPTVGQPNGLGQRVGGAVVGRLPGNRNHPLAAHRASLAKAMARGNQEMEAKILER
LEDPVTKYSRLSLTSVDIFKNLKNGNIKGIWTVCTNPMVAFPNLNMVHEALRKADLVVVQDIYPTETMAFADVVLPAAAI
SGEAYGTYLNSERRVQILEKAVEPPGDALPDEIIHLAFVYKYQKALAEEGRQEDADFLKFLLEPIIQGYEPLFENVQANI
RELREAHQEIQKRLFSELAYVSKGIPGNDLSGLSYERLRTEKDAKGYVGFKLPVLDSSHKGLERLYDENYEAIYQKRFAT
PDGKLRCFLFEQVHPAEEWIDAEYPFMLTLPRIYEHWHTRTRTGRCALPHRLRPEAWLSINPNDAKKLGIKDGDWVEVES
RRGKVTVKAKIDTVKTPREGVVWMPWAYGFLGNLFTGQKEAPPGKSAGNVLSTDAYDPVSKQPELKFAAVKIRKV
>Mature_874_residues
GLKAVGKSRWIENFSQDIAEAANLPAEKWVNSVCPYCGAGCSILYGVREGKVVSVRGNPEAWNRGGVCIKGATLADLYLS
NSPGVKDRLKKPLLRDPEKRGSLENFREVSWDEALNYMVKKVKEYTKTKFGDKHPFAMYGSGQVSTEFMYLQAKFNVIGM
NIHNDNNGRLCQATKANAMVISFGIDGPPMSFDDVFEADNLFIFGFNIADTLPGWFGKIVEAKVQKGDNLKIAVINPVKI
SATQILNYKAGDLYLPIIPTTDVAFINSMAHVIIYELEGVNRDYAGDVSRWADDVISGKVKPKHIDTEFIAKYVNFYRGD
YQVLARLGKEGPKIFEEIKLPSGLEGFKEYAKFLAAYKPEDVAKTTGLTPEDIRKAAQMFVRNKNTMSMYLQGFGQQSNG
VSKHLALVTLHAVTGRIGRAGAGVMPTVGQPNGLGQRVGGAVVGRLPGNRNHPLAAHRASLAKAMARGNQEMEAKILERL
EDPVTKYSRLSLTSVDIFKNLKNGNIKGIWTVCTNPMVAFPNLNMVHEALRKADLVVVQDIYPTETMAFADVVLPAAAIS
GEAYGTYLNSERRVQILEKAVEPPGDALPDEIIHLAFVYKYQKALAEEGRQEDADFLKFLLEPIIQGYEPLFENVQANIR
ELREAHQEIQKRLFSELAYVSKGIPGNDLSGLSYERLRTEKDAKGYVGFKLPVLDSSHKGLERLYDENYEAIYQKRFATP
DGKLRCFLFEQVHPAEEWIDAEYPFMLTLPRIYEHWHTRTRTGRCALPHRLRPEAWLSINPNDAKKLGIKDGDWVEVESR
RGKVTVKAKIDTVKTPREGVVWMPWAYGFLGNLFTGQKEAPPGKSAGNVLSTDAYDPVSKQPELKFAAVKIRKV

Specific function: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and peripla

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788534, Length=874, Percent_Identity=29.8627002288329, Blast_Score=308, Evalue=7e-85,
Organism=Escherichia coli, GI3868721, Length=854, Percent_Identity=27.4004683840749, Blast_Score=257, Evalue=2e-69,
Organism=Escherichia coli, GI3868720, Length=647, Percent_Identity=24.5749613601236, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI3868719, Length=657, Percent_Identity=22.9832572298326, Blast_Score=105, Evalue=2e-23,
Organism=Escherichia coli, GI87081994, Length=509, Percent_Identity=25.147347740668, Blast_Score=70, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR010051
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 97648; Mature: 97517

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLKAVGKSRWIENFSQDIAEAANLPAEKWVNSVCPYCGAGCSILYGVREGKVVSVRGNP
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHCCCCCEEEEECCCCEEEEEECCC
EAWNRGGVCIKGATLADLYLSNSPGVKDRLKKPLLRDPEKRGSLENFREVSWDEALNYMV
CCCCCCCEEEECCEEEEEEECCCCCHHHHHHCCHHCCCHHCCCHHHHHHCCHHHHHHHHH
KKVKEYTKTKFGDKHPFAMYGSGQVSTEFMYLQAKFNVIGMNIHNDNNGRLCQATKANAM
HHHHHHHHHCCCCCCCEEEECCCCCEEEEEEEEEEEEEEEEEEEECCCCCEEEECCCCEE
VISFGIDGPPMSFDDVFEADNLFIFGFNIADTLPGWFGKIVEAKVQKGDNLKIAVINPVK
EEEECCCCCCCCHHHHHCCCCEEEEEECHHHHCCHHHHHHHHHHHCCCCCEEEEEECCEE
ISATQILNYKAGDLYLPIIPTTDVAFINSMAHVIIYELEGVNRDYAGDVSRWADDVISGK
EEHHHHEEECCCCEEEEEECCCHHHHHHHHHHEEEEEECCCCCCCCCCHHHHHHHHHCCC
VKPKHIDTEFIAKYVNFYRGDYQVLARLGKEGPKIFEEIKLPSGLEGFKEYAKFLAAYKP
CCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCC
EDVAKTTGLTPEDIRKAAQMFVRNKNTMSMYLQGFGQQSNGVSKHLALVTLHAVTGRIGR
CHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCC
AGAGVMPTVGQPNGLGQRVGGAVVGRLPGNRNHPLAAHRASLAKAMARGNQEMEAKILER
CCCCCCCCCCCCCCCHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
LEDPVTKYSRLSLTSVDIFKNLKNGNIKGIWTVCTNPMVAFPNLNMVHEALRKADLVVVQ
HHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEECCCEEECCCHHHHHHHHHHCCEEEEE
DIYPTETMAFADVVLPAAAISGEAYGTYLNSERRVQILEKAVEPPGDALPDEIIHLAFVY
CCCCCHHHHHHHHHHHHHHHCCCHHEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHH
KYQKALAEEGRQEDADFLKFLLEPIIQGYEPLFENVQANIRELREAHQEIQKRLFSELAY
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSKGIPGNDLSGLSYERLRTEKDAKGYVGFKLPVLDSSHKGLERLYDENYEAIYQKRFAT
HHCCCCCCCCCCCCHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHCCC
PDGKLRCFLFEQVHPAEEWIDAEYPFMLTLPRIYEHWHTRTRTGRCALPHRLRPEAWLSI
CCCCEEEEEECCCCCHHHHCCCCCCEEEECHHHHHHHHCCCCCCCCCCCCCCCCCEEEEE
NPNDAKKLGIKDGDWVEVESRRGKVTVKAKIDTVKTPREGVVWMPWAYGFLGNLFTGQKE
CCCCHHCCCCCCCCEEEEECCCCEEEEEEEECCCCCCCCCEEECCHHHHHHHHHCCCCCC
APPGKSAGNVLSTDAYDPVSKQPELKFAAVKIRKV
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECC
>Mature Secondary Structure 
GLKAVGKSRWIENFSQDIAEAANLPAEKWVNSVCPYCGAGCSILYGVREGKVVSVRGNP
CCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHCCCCCEEEEECCCCEEEEEECCC
EAWNRGGVCIKGATLADLYLSNSPGVKDRLKKPLLRDPEKRGSLENFREVSWDEALNYMV
CCCCCCCEEEECCEEEEEEECCCCCHHHHHHCCHHCCCHHCCCHHHHHHCCHHHHHHHHH
KKVKEYTKTKFGDKHPFAMYGSGQVSTEFMYLQAKFNVIGMNIHNDNNGRLCQATKANAM
HHHHHHHHHCCCCCCCEEEECCCCCEEEEEEEEEEEEEEEEEEEECCCCCEEEECCCCEE
VISFGIDGPPMSFDDVFEADNLFIFGFNIADTLPGWFGKIVEAKVQKGDNLKIAVINPVK
EEEECCCCCCCCHHHHHCCCCEEEEEECHHHHCCHHHHHHHHHHHCCCCCEEEEEECCEE
ISATQILNYKAGDLYLPIIPTTDVAFINSMAHVIIYELEGVNRDYAGDVSRWADDVISGK
EEHHHHEEECCCCEEEEEECCCHHHHHHHHHHEEEEEECCCCCCCCCCHHHHHHHHHCCC
VKPKHIDTEFIAKYVNFYRGDYQVLARLGKEGPKIFEEIKLPSGLEGFKEYAKFLAAYKP
CCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCC
EDVAKTTGLTPEDIRKAAQMFVRNKNTMSMYLQGFGQQSNGVSKHLALVTLHAVTGRIGR
CHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCC
AGAGVMPTVGQPNGLGQRVGGAVVGRLPGNRNHPLAAHRASLAKAMARGNQEMEAKILER
CCCCCCCCCCCCCCCHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
LEDPVTKYSRLSLTSVDIFKNLKNGNIKGIWTVCTNPMVAFPNLNMVHEALRKADLVVVQ
HHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEECCCEEECCCHHHHHHHHHHCCEEEEE
DIYPTETMAFADVVLPAAAISGEAYGTYLNSERRVQILEKAVEPPGDALPDEIIHLAFVY
CCCCCHHHHHHHHHHHHHHHCCCHHEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHH
KYQKALAEEGRQEDADFLKFLLEPIIQGYEPLFENVQANIRELREAHQEIQKRLFSELAY
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSKGIPGNDLSGLSYERLRTEKDAKGYVGFKLPVLDSSHKGLERLYDENYEAIYQKRFAT
HHCCCCCCCCCCCCHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHCCC
PDGKLRCFLFEQVHPAEEWIDAEYPFMLTLPRIYEHWHTRTRTGRCALPHRLRPEAWLSI
CCCCEEEEEECCCCCHHHHCCCCCCEEEECHHHHHHHHCCCCCCCCCCCCCCCCCEEEEE
NPNDAKKLGIKDGDWVEVESRRGKVTVKAKIDTVKTPREGVVWMPWAYGFLGNLFTGQKE
CCCCHHCCCCCCCCEEEEECCCCEEEEEEEECCCCCCCCCEEECCHHHHHHHHHCCCCCC
APPGKSAGNVLSTDAYDPVSKQPELKFAAVKIRKV
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10368307; 9367852 [H]