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The map label for this gene is hmeA [H]

Identifier: 78042641

GI number: 78042641

Start: 520850

End: 521461

Strand: Direct

Name: hmeA [H]

Synonym: CHY_0602

Alternate gene names: 78042641

Gene position: 520850-521461 (Clockwise)

Preceding gene: 78043983

Following gene: 78044845

Centisome position: 21.69

GC content: 47.22

Gene sequence:

>612_bases
ATGGCACGTTACGGAATGGTTATTGACCTGCGTAAATGCCGGGGGTGCCGCGGTTGTACCGCGGTTTGTGCCGTGGAAAA
TAATGTTCCGGTAGATAGAACCGGTGAAGAGGCCGGTGAGCAGTTAGCCCGGTGGGCAGCCGGGGAGCTTGATTATTACA
ACATGCGGGTATTAACCGCAACGGTATATGGTCAAATTCGCTCCATACCCATGCCGTGCATGCACTGCGACAACGCACCC
TGTATCGCCGTTTGTCCTGTAAAAGCAAGTTACAAAAGAAAAGACGGGATAGTAGTCATCGATAAATATAAATGTATCGG
CTGCCGTCTTTGCTTAAAGGCCTGTCCCTATCAGGCCCGGTATATACGCAAAGTGCCGGTAAAGGGTGAACCCAAGGGAG
TAGCTGGAAAATGCGAGTTTTGCGCCCATTTGGTAGATAAAGGGGAAAAAACCATGTGCAGTAGAAGTTGTCACTCCGGG
GCGATAAAATTTGGCGACTTAGATGATCCCAATAGTGAAGTAAGCCAGCTTTTAAAGCAAAACGCTAAAAAGGTAAAGAG
GCTTAAACCAGAATATAAAACGTCACCCAGAGTTTATTACATTACCTTTTAG

Upstream 100 bases:

>100_bases
ATCCGCCGGAAATGTCTTAAGTACCGATGCTTACGACCCCGTTTCCAAACAGCCCGAACTAAAATTTGCCGCGGTGAAAA
TAAGAAAGGTGTGATGACGG

Downstream 100 bases:

>100_bases
GGGGGAGAGCGGTGAAAAAAAAGCTCCGGCTTTTTATTCTGTTCATTTTTTTAATGGTTGTGGCAACTCCCGTAATGGCC
AAATCCGATAGGAGTTACGG

Product: molybdopterin oxidoreductase, iron-sulfur binding subunit

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: Hme subunit A [H]

Number of amino acids: Translated: 203; Mature: 202

Protein sequence:

>203_residues
MARYGMVIDLRKCRGCRGCTAVCAVENNVPVDRTGEEAGEQLARWAAGELDYYNMRVLTATVYGQIRSIPMPCMHCDNAP
CIAVCPVKASYKRKDGIVVIDKYKCIGCRLCLKACPYQARYIRKVPVKGEPKGVAGKCEFCAHLVDKGEKTMCSRSCHSG
AIKFGDLDDPNSEVSQLLKQNAKKVKRLKPEYKTSPRVYYITF

Sequences:

>Translated_203_residues
MARYGMVIDLRKCRGCRGCTAVCAVENNVPVDRTGEEAGEQLARWAAGELDYYNMRVLTATVYGQIRSIPMPCMHCDNAP
CIAVCPVKASYKRKDGIVVIDKYKCIGCRLCLKACPYQARYIRKVPVKGEPKGVAGKCEFCAHLVDKGEKTMCSRSCHSG
AIKFGDLDDPNSEVSQLLKQNAKKVKRLKPEYKTSPRVYYITF
>Mature_202_residues
ARYGMVIDLRKCRGCRGCTAVCAVENNVPVDRTGEEAGEQLARWAAGELDYYNMRVLTATVYGQIRSIPMPCMHCDNAPC
IAVCPVKASYKRKDGIVVIDKYKCIGCRLCLKACPYQARYIRKVPVKGEPKGVAGKCEFCAHLVDKGEKTMCSRSCHSGA
IKFGDLDDPNSEVSQLLKQNAKKVKRLKPEYKTSPRVYYITF

Specific function: Has menaquinol-oxidizing activity. HmeA, hmeB and hmeE subunits may together catalyze electron transfer from menaquinol to cytochrome c [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Extracellular side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=203, Percent_Identity=36.4532019704434, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI226510944, Length=180, Percent_Identity=35.5555555555556, Blast_Score=106, Evalue=9e-25,
Organism=Escherichia coli, GI1787122, Length=168, Percent_Identity=36.9047619047619, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1787872, Length=168, Percent_Identity=35.1190476190476, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1787740, Length=117, Percent_Identity=39.3162393162393, Blast_Score=80, Evalue=6e-17,
Organism=Escherichia coli, GI1787478, Length=131, Percent_Identity=35.8778625954198, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1789370, Length=203, Percent_Identity=32.0197044334975, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1787749, Length=188, Percent_Identity=27.1276595744681, Blast_Score=70, Evalue=7e-14,
Organism=Escherichia coli, GI2367245, Length=168, Percent_Identity=29.1666666666667, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790326, Length=173, Percent_Identity=27.1676300578035, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87082179, Length=159, Percent_Identity=28.3018867924528, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1788811, Length=165, Percent_Identity=28.4848484848485, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI87082180, Length=130, Percent_Identity=30.7692307692308, Blast_Score=61, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 22661; Mature: 22530

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
10.8 %Cys+Met (Translated Protein)
7.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
10.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARYGMVIDLRKCRGCRGCTAVCAVENNVPVDRTGEEAGEQLARWAAGELDYYNMRVLTA
CCCCCEEEEHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEE
TVYGQIRSIPMPCMHCDNAPCIAVCPVKASYKRKDGIVVIDKYKCIGCRLCLKACPYQAR
EEHHHHHCCCCCCEECCCCCEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCHH
YIRKVPVKGEPKGVAGKCEFCAHLVDKGEKTMCSRSCHSGAIKFGDLDDPNSEVSQLLKQ
HEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCCCCHHHHHHHHHH
NAKKVKRLKPEYKTSPRVYYITF
HHHHHHHCCCCCCCCCEEEEEEC
>Mature Secondary Structure 
ARYGMVIDLRKCRGCRGCTAVCAVENNVPVDRTGEEAGEQLARWAAGELDYYNMRVLTA
CCCCEEEEHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEE
TVYGQIRSIPMPCMHCDNAPCIAVCPVKASYKRKDGIVVIDKYKCIGCRLCLKACPYQAR
EEHHHHHCCCCCCEECCCCCEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCHH
YIRKVPVKGEPKGVAGKCEFCAHLVDKGEKTMCSRSCHSGAIKFGDLDDPNSEVSQLLKQ
HEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCCCCHHHHHHHHHH
NAKKVKRLKPEYKTSPRVYYITF
HHHHHHHCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9389475; 11952791 [H]