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The map label for this gene is ald [H]

Identifier: 78043495

GI number: 78043495

Start: 578428

End: 579546

Strand: Direct

Name: ald [H]

Synonym: CHY_0666

Alternate gene names: 78043495

Gene position: 578428-579546 (Clockwise)

Preceding gene: 78043971

Following gene: 78042901

Centisome position: 24.09

GC content: 49.96

Gene sequence:

>1119_bases
ATGATTATTGGTGTACCAAAAGAAATTAAAAATAATGAAAACCGGGTGGCGATTACTCCTGCCGGAGTTGAGGCTTTGGT
TGCGGCGGGGCATAAAGTGGTGATTGAAAATAACGCCGGTCTTGGCAGCGGGATAACCAATGAAGAATACATAAAAGCTG
GGGCGGAAATTCTTCCAACTGCTGCCGATGTCTTTGCTGCTGCAGATATGATTATGAAGGTAAAGGAGCCACTGCCACCG
GAATACCCGTTATTTAAGGAAGGGCAAATTCTCTTTACTTATCTTCATCTCGCGCCGGAGCCGGAACTTACCCGGGCTTT
AATGGAGAAAAAGGTTGTAGCTATTGCTTACGAAACCATTCAGCTTCCCAACGGCAGCCTGCCATTACTAACTCCAATGA
GCGAGGTTGCCGGGCGGATGGCGGTCCAGATCGGTGCTCGTTTCCTGGAAAAGCCTCAGGGCGGTCGGGGTATGCTATTG
GGGGGCGTTCCCGGAGTTCCACCGGCCGAAGTGGTGATTATTGGTGGCGGTGTCGTCGGTACCAATGCTGCTAAGATGGC
TATGGGCATGGGAGCTCACGTGACCATTCTTGATAAGAGTGCCGACCGGCTTAGATACCTGGATGATATCTTTTTCGGCA
AAATAACCACCATGATGTCCAACAGCTACAATATTGCCGAAGCGGTGAAAAAAGCTGACTTATTGATCGGCGCCGTCTTA
ATTCCGGGAGCCCGGGCGCCCAAATTGGTAACGGAAGAGATGGTTAAGACCATGAAACCCGGTTCGGTTATCGTTGACGT
GGCTATTGACCAGGGTGGTTGCGTCGAAACTATTGACCGGGTAACTACCCACCAGGATCCGATTTACGTTAAACACGGAG
TTGTCCATTATGCTGTAGCCAACATGCCCGGTGCCGTACCGAGAACTTCAACTTTTGCTTTAACCAACGTGACCTTGCCG
TATGCTTTGGCTATTGCCAATAAAGGCTGGGAGCGGGCGGTAAGGGAAGACCGGGCTTTAGCTTTAGGCGTTAACGTTTT
AGACGGAAAAGTTACCTGTAAAGCGGTAGCCGATTCTTTGGGCTTACCTTATACTCCGCTGGAAGAAATCCTCGGTTAG

Upstream 100 bases:

>100_bases
TTTTTAACGCCAACTGATATTGGTACGAAAATTGCTTAAAATAAATATAGCATAAAAATAGCATTTGTTTTAACATAAAA
AATTTGGGGGTGAATTTTGC

Downstream 100 bases:

>100_bases
AGGTAAAAGGGGTGGATAATTCCACCCCTTTGCTATATCATTAACATTAAAAAAGAAAAGTTTTCTGGTAGGTGGAAAAA
ATGCGTCCGGTATGGGCAGA

Product: alanine dehydrogenase

Products: NA

Alternate protein names: Stage V sporulation protein N [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MIIGVPKEIKNNENRVAITPAGVEALVAAGHKVVIENNAGLGSGITNEEYIKAGAEILPTAADVFAAADMIMKVKEPLPP
EYPLFKEGQILFTYLHLAPEPELTRALMEKKVVAIAYETIQLPNGSLPLLTPMSEVAGRMAVQIGARFLEKPQGGRGMLL
GGVPGVPPAEVVIIGGGVVGTNAAKMAMGMGAHVTILDKSADRLRYLDDIFFGKITTMMSNSYNIAEAVKKADLLIGAVL
IPGARAPKLVTEEMVKTMKPGSVIVDVAIDQGGCVETIDRVTTHQDPIYVKHGVVHYAVANMPGAVPRTSTFALTNVTLP
YALAIANKGWERAVREDRALALGVNVLDGKVTCKAVADSLGLPYTPLEEILG

Sequences:

>Translated_372_residues
MIIGVPKEIKNNENRVAITPAGVEALVAAGHKVVIENNAGLGSGITNEEYIKAGAEILPTAADVFAAADMIMKVKEPLPP
EYPLFKEGQILFTYLHLAPEPELTRALMEKKVVAIAYETIQLPNGSLPLLTPMSEVAGRMAVQIGARFLEKPQGGRGMLL
GGVPGVPPAEVVIIGGGVVGTNAAKMAMGMGAHVTILDKSADRLRYLDDIFFGKITTMMSNSYNIAEAVKKADLLIGAVL
IPGARAPKLVTEEMVKTMKPGSVIVDVAIDQGGCVETIDRVTTHQDPIYVKHGVVHYAVANMPGAVPRTSTFALTNVTLP
YALAIANKGWERAVREDRALALGVNVLDGKVTCKAVADSLGLPYTPLEEILG
>Mature_372_residues
MIIGVPKEIKNNENRVAITPAGVEALVAAGHKVVIENNAGLGSGITNEEYIKAGAEILPTAADVFAAADMIMKVKEPLPP
EYPLFKEGQILFTYLHLAPEPELTRALMEKKVVAIAYETIQLPNGSLPLLTPMSEVAGRMAVQIGARFLEKPQGGRGMLL
GGVPGVPPAEVVIIGGGVVGTNAAKMAMGMGAHVTILDKSADRLRYLDDIFFGKITTMMSNSYNIAEAVKKADLLIGAVL
IPGARAPKLVTEEMVKTMKPGSVIVDVAIDQGGCVETIDRVTTHQDPIYVKHGVVHYAVANMPGAVPRTSTFALTNVTLP
YALAIANKGWERAVREDRALALGVNVLDGKVTCKAVADSLGLPYTPLEEILG

Specific function: This enzyme is a key factor in the assimilation of L- alanine as an energy source through the tricarboxylic acid cycle during sporulation [H]

COG id: COG0686

COG function: function code E; Alanine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=386, Percent_Identity=31.6062176165803, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI122939153, Length=386, Percent_Identity=31.6062176165803, Blast_Score=156, Evalue=4e-38,
Organism=Escherichia coli, GI1787887, Length=379, Percent_Identity=31.3984168865435, Blast_Score=139, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17550456, Length=373, Percent_Identity=32.171581769437, Blast_Score=159, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR008141 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.4.1.1 [H]

Molecular weight: Translated: 39377; Mature: 39377

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIGVPKEIKNNENRVAITPAGVEALVAAGHKVVIENNAGLGSGITNEEYIKAGAEILPT
CEECCCHHHCCCCCEEEEECCCHHHHHHCCCEEEEECCCCCCCCCCCHHHHHCCHHHCCC
AADVFAAADMIMKVKEPLPPEYPLFKEGQILFTYLHLAPEPELTRALMEKKVVAIAYETI
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHEEEEEEEEE
QLPNGSLPLLTPMSEVAGRMAVQIGARFLEKPQGGRGMLLGGVPGVPPAEVVIIGGGVVG
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEECCCEEC
TNAAKMAMGMGAHVTILDKSADRLRYLDDIFFGKITTMMSNSYNIAEAVKKADLLIGAVL
CCHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEHEE
IPGARAPKLVTEEMVKTMKPGSVIVDVAIDQGGCVETIDRVTTHQDPIYVKHGVVHYAVA
CCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCCCEEEECCEEEEEEC
NMPGAVPRTSTFALTNVTLPYALAIANKGWERAVREDRALALGVNVLDGKVTCKAVADSL
CCCCCCCCCCEEEEEECCHHEEEEECCCCHHHHHHHCCEEEEEEEEECCCEEHHHHHHHC
GLPYTPLEEILG
CCCCCCHHHHCC
>Mature Secondary Structure
MIIGVPKEIKNNENRVAITPAGVEALVAAGHKVVIENNAGLGSGITNEEYIKAGAEILPT
CEECCCHHHCCCCCEEEEECCCHHHHHHCCCEEEEECCCCCCCCCCCHHHHHCCHHHCCC
AADVFAAADMIMKVKEPLPPEYPLFKEGQILFTYLHLAPEPELTRALMEKKVVAIAYETI
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHEEEEEEEEE
QLPNGSLPLLTPMSEVAGRMAVQIGARFLEKPQGGRGMLLGGVPGVPPAEVVIIGGGVVG
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEECCCEEC
TNAAKMAMGMGAHVTILDKSADRLRYLDDIFFGKITTMMSNSYNIAEAVKKADLLIGAVL
CCHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEHEE
IPGARAPKLVTEEMVKTMKPGSVIVDVAIDQGGCVETIDRVTTHQDPIYVKHGVVHYAVA
CCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCCCEEEECCEEEEEEC
NMPGAVPRTSTFALTNVTLPYALAIANKGWERAVREDRALALGVNVLDGKVTCKAVADSL
CCCCCCCCCCEEEEEECCHHEEEEECCCCHHHHHHHCCEEEEEEEEECCCEEHHHHHHHC
GLPYTPLEEILG
CCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226620; 9384377 [H]