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The map label for this gene is aes [H]

Identifier: 77465682

GI number: 77465682

Start: 794257

End: 795174

Strand: Reverse

Name: aes [H]

Synonym: RSP_3675

Alternate gene names: 77465682

Gene position: 795174-794257 (Counterclockwise)

Preceding gene: 77465683

Following gene: 77465681

Centisome position: 84.32

GC content: 72.98

Gene sequence:

>918_bases
ATGAGCCTGACCCTTGCTCCCGAAATGCGCGCCGTGCTCGACCGGCTCTTGGCCGAGGACGGGGTGCAGCCCGATCCGAC
GCTCCTGCCGCCGGCCGAGGGCCGGGCGGCGGCCGAGGCCGGCAACCGGCGCTGGAACGGGCCGGAGCCCGCCATGGCGC
GCGAGCTTCGCGTCGAGACGCCGGACGGGCGGCGGGCGCATGTCTTCCTGCCCGCGGGCGATCTCGGGCGCGAGGCGATC
CTCTTCATCCACGGCGGCGGCTGGGCCTTCTGCTCGGCCGCGACCCACGAGGGGGCGGCGCGCCAGCTTGCGGTCGAGAC
CGGCGCGGCGGTGGTGACTTTCGACTACCGGCTCGCGCCGGAACATCCCTATCCGGCGGGACTGGAGGATTGTCTCGCCG
TCTGGGCCGGGCGCGAGGCGATCCTCGCCGGACGCCGCTGGTCGGTGGCGGGCGATAGCGCGGGCGCCCATCTCGCGGTC
GCGCTGATGTTCCGGCTGAACGGCCGCGACCTGCCGGCGACGGGGCTCCTCTTCTACGGGGTGTTCGATGCCGATTTCGA
AAGCCCGAGCTATGTCGAGGTGGCCGAGGGGCCGGGCCTGACCCGCGCCAAGATGCTGCGCTACTGGGATTTCTACACGC
CCGCGGCAGGCCGGCTCGAGGATCCCTGTCTCACGCCCGCCCGCGCGCCGGATGCGATGCTGGCGGCGCTGCCGCCGCTC
TATCTCAACGCGGCCGAGATCGATCCCCTGCGCTCGGACGCCGAGCGCTTCTCGGCGCGGCTCACAGCGTTGGGGCGGAC
CGACAGGTTCGACATCGTGCCGGGGGTCGTCCACGGATTCATGCAGATGAGCCGTTGGCTGCCGCAGTCGGTGGCGGCCT
ACCGCCGGGCGGGCGAGGTGTTCCGGGCGCTCACCTGA

Upstream 100 bases:

>100_bases
ATCACATCGCGACCATCGTCGATTTCGGCCCCGAGGTCATGCCGACGCTCGCGCCCGAGGCAGGGCAGGGCATGGCGACC
CCCGCCCTGAAGGTGCATCC

Downstream 100 bases:

>100_bases
CCGCCGCCCCGGGCGGCGAACGTTTCTCGATTTTGGGAGGAGAGAGAATGAAAAGAACCATCAGCGCGCTGGCCCTTCTG
GCCGGCCTCGCGCCGGGGAT

Product: esterase/lipase/thioesterase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MSLTLAPEMRAVLDRLLAEDGVQPDPTLLPPAEGRAAAEAGNRRWNGPEPAMARELRVETPDGRRAHVFLPAGDLGREAI
LFIHGGGWAFCSAATHEGAARQLAVETGAAVVTFDYRLAPEHPYPAGLEDCLAVWAGREAILAGRRWSVAGDSAGAHLAV
ALMFRLNGRDLPATGLLFYGVFDADFESPSYVEVAEGPGLTRAKMLRYWDFYTPAAGRLEDPCLTPARAPDAMLAALPPL
YLNAAEIDPLRSDAERFSARLTALGRTDRFDIVPGVVHGFMQMSRWLPQSVAAYRRAGEVFRALT

Sequences:

>Translated_305_residues
MSLTLAPEMRAVLDRLLAEDGVQPDPTLLPPAEGRAAAEAGNRRWNGPEPAMARELRVETPDGRRAHVFLPAGDLGREAI
LFIHGGGWAFCSAATHEGAARQLAVETGAAVVTFDYRLAPEHPYPAGLEDCLAVWAGREAILAGRRWSVAGDSAGAHLAV
ALMFRLNGRDLPATGLLFYGVFDADFESPSYVEVAEGPGLTRAKMLRYWDFYTPAAGRLEDPCLTPARAPDAMLAALPPL
YLNAAEIDPLRSDAERFSARLTALGRTDRFDIVPGVVHGFMQMSRWLPQSVAAYRRAGEVFRALT
>Mature_304_residues
SLTLAPEMRAVLDRLLAEDGVQPDPTLLPPAEGRAAAEAGNRRWNGPEPAMARELRVETPDGRRAHVFLPAGDLGREAIL
FIHGGGWAFCSAATHEGAARQLAVETGAAVVTFDYRLAPEHPYPAGLEDCLAVWAGREAILAGRRWSVAGDSAGAHLAVA
LMFRLNGRDLPATGLLFYGVFDADFESPSYVEVAEGPGLTRAKMLRYWDFYTPAAGRLEDPCLTPARAPDAMLAALPPLY
LNAAEIDPLRSDAERFSARLTALGRTDRFDIVPGVVHGFMQMSRWLPQSVAAYRRAGEVFRALT

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=168, Percent_Identity=31.547619047619, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI68051721, Length=281, Percent_Identity=25.9786476868327, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI21328446, Length=94, Percent_Identity=38.2978723404255, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1786682, Length=319, Percent_Identity=28.5266457680251, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17567059, Length=176, Percent_Identity=32.9545454545455, Blast_Score=88, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 33036; Mature: 32905

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTLAPEMRAVLDRLLAEDGVQPDPTLLPPAEGRAAAEAGNRRWNGPEPAMARELRVET
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHEEEEC
PDGRRAHVFLPAGDLGREAILFIHGGGWAFCSAATHEGAARQLAVETGAAVVTFDYRLAP
CCCCEEEEEECCCCCCCEEEEEEECCCEEEEECCCCCCHHHEEEEECCCEEEEEEEEECC
EHPYPAGLEDCLAVWAGREAILAGRRWSVAGDSAGAHLAVALMFRLNGRDLPATGLLFYG
CCCCCCCHHHHHHHHCCCHHEEECCEEECCCCCCCHHHEEEEEHHCCCCCCCCCCEEEEE
VFDADFESPSYVEVAEGPGLTRAKMLRYWDFYTPAAGRLEDPCLTPARAPDAMLAALPPL
EEECCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCCH
YLNAAEIDPLRSDAERFSARLTALGRTDRFDIVPGVVHGFMQMSRWLPQSVAAYRRAGEV
HCCHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHH
FRALT
HHHCC
>Mature Secondary Structure 
SLTLAPEMRAVLDRLLAEDGVQPDPTLLPPAEGRAAAEAGNRRWNGPEPAMARELRVET
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHEEEEC
PDGRRAHVFLPAGDLGREAILFIHGGGWAFCSAATHEGAARQLAVETGAAVVTFDYRLAP
CCCCEEEEEECCCCCCCEEEEEEECCCEEEEECCCCCCHHHEEEEECCCEEEEEEEEECC
EHPYPAGLEDCLAVWAGREAILAGRRWSVAGDSAGAHLAVALMFRLNGRDLPATGLLFYG
CCCCCCCHHHHHHHHCCCHHEEECCEEECCCCCCCHHHEEEEEHHCCCCCCCCCCEEEEE
VFDADFESPSYVEVAEGPGLTRAKMLRYWDFYTPAAGRLEDPCLTPARAPDAMLAALPPL
EEECCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCCH
YLNAAEIDPLRSDAERFSARLTALGRTDRFDIVPGVVHGFMQMSRWLPQSVAAYRRAGEV
HCCHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHH
FRALT
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA