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The map label for this gene is ramA [H]

Identifier: 77465667

GI number: 77465667

Start: 767805

End: 768647

Strand: Reverse

Name: ramA [H]

Synonym: RSP_3659

Alternate gene names: 77465667

Gene position: 768647-767805 (Counterclockwise)

Preceding gene: 77465668

Following gene: 77465666

Centisome position: 81.51

GC content: 68.33

Gene sequence:

>843_bases
ATGAAAATCGCATTCGCCCAGCTGTCGCCCGTGCACGGCGACACCCCTGCCACCGTCGCGCTCGTCGCGGAGGCCGCCCG
CGCCGCGGCGGCCGACGGGGCACGGCTCATCGTCTTTCCCGAGTGCTTCCTCACCGGCGGCTCGTTCGACGACCGCGCCG
CGCTGCTGCAGGCGGCCGTCGACATCGAGCGGGGCGATCTCGCACCCATTCTTCTGGCGGCCCGCGAGGCGGACATCCAT
GTCGTCGTGGGCTTCTATCAGAAGTCCGGCCCGCAGGCCCTGAACACCGCGGCCCTGATCGGGCCCGAGGGCATCATCGG
CCTGCATCACAAGATGCACCTGCCGTTCATGATCGGCGACCGCTTCGCCGACATCCCCCAGATCGAGGGCCCGTCGGTCT
TCGACACGGCGATCGGCCGCATCGGCCTCGCGATCTGTTACGAGATCCGCTTCCCCGAGGTGATCCGCACGCTGGCGCTG
GAAGGCGCCGAACTCGTGGTGCTGCCCGCCGCCTGGCCCGAGGCCGCCCGCATCCTGCCCGACCTCTTCAGCCGCGTGCG
CGCCGCCGAGAACTTCGTCTATTTCCTGTCCTCGAACCGCATCGACGTCGATGACGGCATGGCCTTCATGGGCAGCAGCC
ACGTCATCGGCCCGGACGGCAACGAGATCTTCAATGCCGGCATGCAGGAAGGCATCTTCACCGTCGATATCGACCTTGCG
CGCGCGCGCACCAAGTCGATCATCCGCGATCCCGGCGTCTACGAGGTGCATCCCTTCCGCGACCGCCGCCCGCAGGATTA
CCGCATCTGCGCCGCCCCCGCAGCACTCTGGCTCGAGGCATGA

Upstream 100 bases:

>100_bases
CGAGCTCGTGCGCACCGTCTTCGATGCGGTCGGCTTCTCGCTGGATTGAGCCCGGCCGGGACCCGCGCCGCGGGTCCCGC
TCCCGCAGAATGGAGTGGAC

Downstream 100 bases:

>100_bases
GCCCCGAGTGGCGCGACAGGCTCGCCGCGGGCGGGCCGGTCTTCGGCATGAACGTCTATTCGGGCAGCCCCTCCGCGGTC
GAGATCGCGGGCGCCTGGGG

Product: amidohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MKIAFAQLSPVHGDTPATVALVAEAARAAAADGARLIVFPECFLTGGSFDDRAALLQAAVDIERGDLAPILLAAREADIH
VVVGFYQKSGPQALNTAALIGPEGIIGLHHKMHLPFMIGDRFADIPQIEGPSVFDTAIGRIGLAICYEIRFPEVIRTLAL
EGAELVVLPAAWPEAARILPDLFSRVRAAENFVYFLSSNRIDVDDGMAFMGSSHVIGPDGNEIFNAGMQEGIFTVDIDLA
RARTKSIIRDPGVYEVHPFRDRRPQDYRICAAPAALWLEA

Sequences:

>Translated_280_residues
MKIAFAQLSPVHGDTPATVALVAEAARAAAADGARLIVFPECFLTGGSFDDRAALLQAAVDIERGDLAPILLAAREADIH
VVVGFYQKSGPQALNTAALIGPEGIIGLHHKMHLPFMIGDRFADIPQIEGPSVFDTAIGRIGLAICYEIRFPEVIRTLAL
EGAELVVLPAAWPEAARILPDLFSRVRAAENFVYFLSSNRIDVDDGMAFMGSSHVIGPDGNEIFNAGMQEGIFTVDIDLA
RARTKSIIRDPGVYEVHPFRDRRPQDYRICAAPAALWLEA
>Mature_280_residues
MKIAFAQLSPVHGDTPATVALVAEAARAAAADGARLIVFPECFLTGGSFDDRAALLQAAVDIERGDLAPILLAAREADIH
VVVGFYQKSGPQALNTAALIGPEGIIGLHHKMHLPFMIGDRFADIPQIEGPSVFDTAIGRIGLAICYEIRFPEVIRTLAL
EGAELVVLPAAWPEAARILPDLFSRVRAAENFVYFLSSNRIDVDDGMAFMGSSHVIGPDGNEIFNAGMQEGIFTVDIDLA
RARTKSIIRDPGVYEVHPFRDRRPQDYRICAAPAALWLEA

Specific function: Hydrolyzes (R)-piperazine-2-carboxamide and (R)- piperazine-2-tert-butylcarboxamide with strict R- stereoselectivity. Also active towards beta-alaninamide, piperidine-3-carboxmide, D-glutaminamide and slightly active towards L-glutaminamide and piperidine

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=257, Percent_Identity=29.1828793774319, Blast_Score=88, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6323383, Length=257, Percent_Identity=29.1828793774319, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21358471, Length=271, Percent_Identity=26.5682656826568, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.100 [H]

Molecular weight: Translated: 30257; Mature: 30257

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAFAQLSPVHGDTPATVALVAEAARAAAADGARLIVFPECFLTGGSFDDRAALLQAAV
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECHHEEECCCCCHHHHHHHHHH
DIERGDLAPILLAAREADIHVVVGFYQKSGPQALNTAALIGPEGIIGLHHKMHLPFMIGD
CCCCCCCCEEEEEECCCCEEEEEEEEECCCCCHHCCEEEECCCCEEECCCEEECCEEECC
RFADIPQIEGPSVFDTAIGRIGLAICYEIRFPEVIRTLALEGAELVVLPAAWPEAARILP
CCCCCCCCCCCCHHHHHHHHHEEEEEEECCCHHHHHHHHCCCCCEEEEECCCCHHHHHHH
DLFSRVRAAENFVYFLSSNRIDVDDGMAFMGSSHVIGPDGNEIFNAGMQEGIFTVDIDLA
HHHHHHHHHCCEEEEEECCEEECCCCEEECCCCEEECCCCHHHHHHHHHCCEEEEEEHHH
RARTKSIIRDPGVYEVHPFRDRRPQDYRICAAPAALWLEA
HHHHHHHHCCCCCEEECCCCCCCCCCEEEEECCHHHEECC
>Mature Secondary Structure
MKIAFAQLSPVHGDTPATVALVAEAARAAAADGARLIVFPECFLTGGSFDDRAALLQAAV
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECHHEEECCCCCHHHHHHHHHH
DIERGDLAPILLAAREADIHVVVGFYQKSGPQALNTAALIGPEGIIGLHHKMHLPFMIGD
CCCCCCCCEEEEEECCCCEEEEEEEEECCCCCHHCCEEEECCCCEEECCCEEECCEEECC
RFADIPQIEGPSVFDTAIGRIGLAICYEIRFPEVIRTLALEGAELVVLPAAWPEAARILP
CCCCCCCCCCCCHHHHHHHHHEEEEEEECCCHHHHHHHHCCCCCEEEEECCCCHHHHHHH
DLFSRVRAAENFVYFLSSNRIDVDDGMAFMGSSHVIGPDGNEIFNAGMQEGIFTVDIDLA
HHHHHHHHHCCEEEEEECCEEECCCCEEECCCCEEECCCCHHHHHHHHHCCEEEEEEHHH
RARTKSIIRDPGVYEVHPFRDRRPQDYRICAAPAALWLEA
HHHHHHHHCCCCCEEECCCCCCCCCCEEEEECCHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA