Click here to switch to the map view.

The map label for this gene is trmB

Identifier: 77465594

GI number: 77465594

Start: 685745

End: 686470

Strand: Reverse

Name: trmB

Synonym: RSP_3593

Alternate gene names: 77465594

Gene position: 686470-685745 (Counterclockwise)

Preceding gene: 77465596

Following gene: 77465593

Centisome position: 72.8

GC content: 69.42

Gene sequence:

>726_bases
ATGAGTGACGACGACGCCCCCGAATCCCTACGCGGCGCGGCGGACGGCCCCGCCTGGCGCAACTTCTATGGCCGGCGGTC
GGGCAAGACGCTGCGCCCCTCGCAGAAGGTCTATCTCTCCGAGGATCTCGGCCGGCTGCGGCCCGAAGGCATCACGCGCG
AGGAGAACCCCGAGCGTCGCCCGCTCGATCTCGGCGCGCTCTTCGGCGGCCGTCCGGTCTGGCTCGAGATCGGCTTCGGC
GGCGGCGAGCACATGGTCCATATGGCTGCGCGCAATCCCGGGATCGGCCTGATCGGCTGCGAGCCCTTCGTGAACGGGGT
GGCGATGCTGCTCGGCAAGATCCGGGCGGCGGGCGTGGAGAATCTCCTCGTGCACCCGGGCGATGTGCGCGATCTCTTCG
ACGTGCTGCCGGAGGCCTCCGTCTCGAAGGCCTTCCTCAACTATCCCGATCCCTGGCCGAAGAAGCGCCACCACCGGCGC
CGGTTCGTGACGCAGGGCTATCTCGGCCCTCTGGCCCGCGTGCTCGCGCCGGGGGCCACGTTCCGCGTTGCGACCGACAT
CCCCGATTATGTGCGCCAGACGCTTGAGGAGGTGCCGCAGGCCGGCTTCGATCTCGTGTCGGAGAGCGGCGAGGCCTGGG
AGGACTGGCTCTCGACCCGCTACGAACAGAAGGCGCTCCGCGAGGGACGTGTGCCGCACTACATGACCTTCCGGCGACAG
GGCTGA

Upstream 100 bases:

>100_bases
CATCCCCCGGTTCCCGAGGCGGCAAAAACTTGCGCGGGGGGCGGTTGACCTGTGGCGCGGGGGCGTTTAGGGGCTGGCGG
AGGCTGCAGAAGGTGCCGAA

Downstream 100 bases:

>100_bases
GCGGCCTTAGAAAAAGACTTTTAACTCAGTGATCTGTGTGCTTTTGCTCGCGTGCTGCATGAGCATGGTGGGATCGGCAC
CCCGCGCTTGTGCGGTCCAC

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MSDDDAPESLRGAADGPAWRNFYGRRSGKTLRPSQKVYLSEDLGRLRPEGITREENPERRPLDLGALFGGRPVWLEIGFG
GGEHMVHMAARNPGIGLIGCEPFVNGVAMLLGKIRAAGVENLLVHPGDVRDLFDVLPEASVSKAFLNYPDPWPKKRHHRR
RFVTQGYLGPLARVLAPGATFRVATDIPDYVRQTLEEVPQAGFDLVSESGEAWEDWLSTRYEQKALREGRVPHYMTFRRQ
G

Sequences:

>Translated_241_residues
MSDDDAPESLRGAADGPAWRNFYGRRSGKTLRPSQKVYLSEDLGRLRPEGITREENPERRPLDLGALFGGRPVWLEIGFG
GGEHMVHMAARNPGIGLIGCEPFVNGVAMLLGKIRAAGVENLLVHPGDVRDLFDVLPEASVSKAFLNYPDPWPKKRHHRR
RFVTQGYLGPLARVLAPGATFRVATDIPDYVRQTLEEVPQAGFDLVSESGEAWEDWLSTRYEQKALREGRVPHYMTFRRQ
G
>Mature_240_residues
SDDDAPESLRGAADGPAWRNFYGRRSGKTLRPSQKVYLSEDLGRLRPEGITREENPERRPLDLGALFGGRPVWLEIGFGG
GEHMVHMAARNPGIGLIGCEPFVNGVAMLLGKIRAAGVENLLVHPGDVRDLFDVLPEASVSKAFLNYPDPWPKKRHHRRR
FVTQGYLGPLARVLAPGATFRVATDIPDYVRQTLEEVPQAGFDLVSESGEAWEDWLSTRYEQKALREGRVPHYMTFRRQG

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=184, Percent_Identity=35.3260869565217, Blast_Score=84, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_RHOS4 (Q3IW88)

Other databases:

- EMBL:   CP000144
- RefSeq:   YP_355097.1
- ProteinModelPortal:   Q3IW88
- SMR:   Q3IW88
- STRING:   Q3IW88
- GeneID:   3722110
- GenomeReviews:   CP000144_GR
- KEGG:   rsp:RSP_3593
- NMPDR:   fig|272943.3.peg.3341
- eggNOG:   COG0220
- HOGENOM:   HBG322782
- OMA:   YEAKALR
- PhylomeDB:   Q3IW88
- ProtClustDB:   PRK00121
- BioCyc:   RSPH272943:RSP_3593-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 26988; Mature: 26857

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: BINDING 76-76 BINDING 101-101 BINDING 128-128 BINDING 150-150 BINDING 154-154 BINDING 186-186

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDDDAPESLRGAADGPAWRNFYGRRSGKTLRPSQKVYLSEDLGRLRPEGITREENPERR
CCCCCCCHHHCCCCCCCHHHHHHHCCCCCCCCCCHHEEEHHHHHHCCCCCCCCCCCCCCC
PLDLGALFGGRPVWLEIGFGGGEHMVHMAARNPGIGLIGCEPFVNGVAMLLGKIRAAGVE
CCHHHHHCCCCEEEEEEECCCCCCEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHCHH
NLLVHPGDVRDLFDVLPEASVSKAFLNYPDPWPKKRHHRRRFVTQGYLGPLARVLAPGAT
HHEECCCCHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
FRVATDIPDYVRQTLEEVPQAGFDLVSESGEAWEDWLSTRYEQKALREGRVPHYMTFRRQ
EEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
G
C
>Mature Secondary Structure 
SDDDAPESLRGAADGPAWRNFYGRRSGKTLRPSQKVYLSEDLGRLRPEGITREENPERR
CCCCCCHHHCCCCCCCHHHHHHHCCCCCCCCCCHHEEEHHHHHHCCCCCCCCCCCCCCC
PLDLGALFGGRPVWLEIGFGGGEHMVHMAARNPGIGLIGCEPFVNGVAMLLGKIRAAGVE
CCHHHHHCCCCEEEEEEECCCCCCEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHCHH
NLLVHPGDVRDLFDVLPEASVSKAFLNYPDPWPKKRHHRRRFVTQGYLGPLARVLAPGAT
HHEECCCCHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
FRVATDIPDYVRQTLEEVPQAGFDLVSESGEAWEDWLSTRYEQKALREGRVPHYMTFRRQ
EEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA