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The map label for this gene is metK

Identifier: 77465596

GI number: 77465596

Start: 687402

End: 688568

Strand: Reverse

Name: metK

Synonym: RSP_3595

Alternate gene names: 77465596

Gene position: 688568-687402 (Counterclockwise)

Preceding gene: 77465597

Following gene: 77465594

Centisome position: 73.02

GC content: 67.78

Gene sequence:

>1167_bases
ATGTCCCGCATGAACTACGTCTTCACTTCCGAGTCCGTCTCGGAAGGTCACCCCGACAAGCTGTGCGATCGCGTGTCGGA
TGCCGTCCTCGATACCTTCCTGGCCGAGGAACCCACCGCCCGGGTCGCCTGCGAAACCTTCGCCACGACCGGCCGCGTCG
TTGTCGGAGGCGAAGTCGGCCTCTCCGATCCCCAGAAGCTGGACGAGTTCATGGAGCGCGTGGACGGCATCGTCCGCGAT
TGCGTGAAGGACATCGGCTACGAGCAGCAGGAATTCCACTGGCGCACCATCGAGGTGCAGAACTTCCTGCACCGCCAGTC
GGCCCATATCGCGCAGGGCGTCGACAAGGACGGCGCGGGCGACCAAGGCATCATGTTCGGCTATGCCTGCCGCGAGACGC
CCGAGCTGATGCCGGCGCCGATCCAGTATTCGCATGCGATCCTGCGCCGGCTGGCCGAGGTGCGCAAATCGGGTCAGGAG
CCCGACCTGCGTCCCGACGCCAAGTCGCAGCTCTCGCTGCGCTACGAGAACGGCAAGCCCGTCGAGGTGCGCTCGATCGT
GCTCTCGACCCAGCACGCCCATGAGGAGCAGACCTCCGACGACATCCGCGCCATCGTCGAGCCCTACATCCGCGAAGTGC
TGCCCGAGGGCTGGATCACCGAGGCGACCGAATGGTGGGTGAACCCAACCGGGACCTTCGTCATCGGCGGGCCGGACGGG
GATGCGGGCCTGACGGGCCGCAAGATCATCGTGGACACCTACGGCGGGGCCGCGCCCCACGGCGGCGGCGCCTTCTCGGG
CAAGGATCCGACCAAGGTCGACCGCTCGGCCGCCTATGCCGCGCGCTACCTCGCCAAGAACGTCGTGGCCGCGGGCCTCG
CCGAGCGCTGCACGCTGCAGGTCTCCTATGCCATCGGCGTGGCGAAGCCGCTCTCGATCTATGTCGACACCCATGGCACG
GGCCAGGTGGATGCGGCGCAGATCGAGAAGGCCGTGGCGGACTGCATGGACCTCACGCCGCGCGGCATCCGCGAGCATCT
GAACCTCTGCCGGCCGATCTATGCCCGCACCTCGGCCTACGGTCACTTCGGCCGGGCGCCGGAAGCGGACGGCGGCTTCT
CGTGGGAACGGACGGACCTGACCGACGCCCTGCTGAAGGCCGTCTGA

Upstream 100 bases:

>100_bases
CGGGGCGCTTGACCCGCCTCCCCTCAGGGGTGTAGCGCGGAGGGCAACCGTCACAACGGCTTCCTGGCGTGACGGGGTTA
ACCCCAACGGAGCACTTCCC

Downstream 100 bases:

>100_bases
GGAACCGGCGGCAAGTCCTTGCCGCATAAGGGCGGATTGCCTCTGACAGACAAAATGAAGCCCCGGCGGATCGCCGGGGC
TTCTGTCGTTCTGAAACTCT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MSRMNYVFTSESVSEGHPDKLCDRVSDAVLDTFLAEEPTARVACETFATTGRVVVGGEVGLSDPQKLDEFMERVDGIVRD
CVKDIGYEQQEFHWRTIEVQNFLHRQSAHIAQGVDKDGAGDQGIMFGYACRETPELMPAPIQYSHAILRRLAEVRKSGQE
PDLRPDAKSQLSLRYENGKPVEVRSIVLSTQHAHEEQTSDDIRAIVEPYIREVLPEGWITEATEWWVNPTGTFVIGGPDG
DAGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYAARYLAKNVVAAGLAERCTLQVSYAIGVAKPLSIYVDTHGT
GQVDAAQIEKAVADCMDLTPRGIREHLNLCRPIYARTSAYGHFGRAPEADGGFSWERTDLTDALLKAV

Sequences:

>Translated_388_residues
MSRMNYVFTSESVSEGHPDKLCDRVSDAVLDTFLAEEPTARVACETFATTGRVVVGGEVGLSDPQKLDEFMERVDGIVRD
CVKDIGYEQQEFHWRTIEVQNFLHRQSAHIAQGVDKDGAGDQGIMFGYACRETPELMPAPIQYSHAILRRLAEVRKSGQE
PDLRPDAKSQLSLRYENGKPVEVRSIVLSTQHAHEEQTSDDIRAIVEPYIREVLPEGWITEATEWWVNPTGTFVIGGPDG
DAGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYAARYLAKNVVAAGLAERCTLQVSYAIGVAKPLSIYVDTHGT
GQVDAAQIEKAVADCMDLTPRGIREHLNLCRPIYARTSAYGHFGRAPEADGGFSWERTDLTDALLKAV
>Mature_387_residues
SRMNYVFTSESVSEGHPDKLCDRVSDAVLDTFLAEEPTARVACETFATTGRVVVGGEVGLSDPQKLDEFMERVDGIVRDC
VKDIGYEQQEFHWRTIEVQNFLHRQSAHIAQGVDKDGAGDQGIMFGYACRETPELMPAPIQYSHAILRRLAEVRKSGQEP
DLRPDAKSQLSLRYENGKPVEVRSIVLSTQHAHEEQTSDDIRAIVEPYIREVLPEGWITEATEWWVNPTGTFVIGGPDGD
AGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYAARYLAKNVVAAGLAERCTLQVSYAIGVAKPLSIYVDTHGTG
QVDAAQIEKAVADCMDLTPRGIREHLNLCRPIYARTSAYGHFGRAPEADGGFSWERTDLTDALLKAV

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=391, Percent_Identity=49.3606138107417, Blast_Score=330, Evalue=1e-90,
Organism=Homo sapiens, GI5174529, Length=388, Percent_Identity=50, Blast_Score=330, Evalue=2e-90,
Organism=Escherichia coli, GI1789311, Length=390, Percent_Identity=54.8717948717949, Blast_Score=412, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17538494, Length=392, Percent_Identity=50, Blast_Score=342, Evalue=3e-94,
Organism=Caenorhabditis elegans, GI25145633, Length=392, Percent_Identity=50.2551020408163, Blast_Score=340, Evalue=7e-94,
Organism=Caenorhabditis elegans, GI17538498, Length=392, Percent_Identity=50, Blast_Score=340, Evalue=9e-94,
Organism=Caenorhabditis elegans, GI17551082, Length=388, Percent_Identity=47.9381443298969, Blast_Score=323, Evalue=7e-89,
Organism=Caenorhabditis elegans, GI32565905, Length=387, Percent_Identity=46.5116279069767, Blast_Score=303, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI32565911, Length=313, Percent_Identity=50.4792332268371, Blast_Score=272, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI25145635, Length=313, Percent_Identity=50.4792332268371, Blast_Score=269, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI32565903, Length=296, Percent_Identity=50.6756756756757, Blast_Score=258, Evalue=4e-69,
Organism=Caenorhabditis elegans, GI32565909, Length=107, Percent_Identity=45.7943925233645, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI32565907, Length=44, Percent_Identity=68.1818181818182, Blast_Score=71, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6320710, Length=385, Percent_Identity=47.7922077922078, Blast_Score=327, Evalue=1e-90,
Organism=Saccharomyces cerevisiae, GI6323209, Length=386, Percent_Identity=48.7046632124352, Blast_Score=323, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24580527, Length=387, Percent_Identity=48.578811369509, Blast_Score=314, Evalue=7e-86,
Organism=Drosophila melanogaster, GI24580525, Length=387, Percent_Identity=48.578811369509, Blast_Score=314, Evalue=7e-86,
Organism=Drosophila melanogaster, GI45552159, Length=387, Percent_Identity=48.8372093023256, Blast_Score=310, Evalue=1e-84,
Organism=Drosophila melanogaster, GI24580529, Length=387, Percent_Identity=48.8372093023256, Blast_Score=310, Evalue=1e-84,
Organism=Drosophila melanogaster, GI24580537, Length=387, Percent_Identity=48.8372093023256, Blast_Score=310, Evalue=1e-84,
Organism=Drosophila melanogaster, GI24580533, Length=387, Percent_Identity=48.8372093023256, Blast_Score=310, Evalue=1e-84,
Organism=Drosophila melanogaster, GI24580535, Length=387, Percent_Identity=48.8372093023256, Blast_Score=310, Evalue=1e-84,
Organism=Drosophila melanogaster, GI24580531, Length=387, Percent_Identity=48.8372093023256, Blast_Score=310, Evalue=1e-84,
Organism=Drosophila melanogaster, GI24580541, Length=374, Percent_Identity=47.5935828877005, Blast_Score=291, Evalue=4e-79,
Organism=Drosophila melanogaster, GI24580539, Length=128, Percent_Identity=42.96875, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_RHOS1 (A3PPW0)

Other databases:

- EMBL:   CP000578
- RefSeq:   YP_001045148.1
- ProteinModelPortal:   A3PPW0
- SMR:   A3PPW0
- STRING:   A3PPW0
- GeneID:   4898283
- GenomeReviews:   CP000578_GR
- KEGG:   rsh:Rsph17029_3280
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   QDGFHWK
- ProtClustDB:   PRK05250
- BioCyc:   RSPH349101:RSPH17029_3280-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 42467; Mature: 42336

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRMNYVFTSESVSEGHPDKLCDRVSDAVLDTFLAEEPTARVACETFATTGRVVVGGEVG
CCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEECCCCC
LSDPQKLDEFMERVDGIVRDCVKDIGYEQQEFHWRTIEVQNFLHRQSAHIAQGVDKDGAG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEHHHHHHHHHHHHHHHCCCCCCCC
DQGIMFGYACRETPELMPAPIQYSHAILRRLAEVRKSGQEPDLRPDAKSQLSLRYENGKP
CCCEEEEEHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCC
VEVRSIVLSTQHAHEEQTSDDIRAIVEPYIREVLPEGWITEATEWWVNPTGTFVIGGPDG
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEECCCCEEEEECCCC
DAGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYAARYLAKNVVAAGLAERCTLQ
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
VSYAIGVAKPLSIYVDTHGTGQVDAAQIEKAVADCMDLTPRGIREHLNLCRPIYARTSAY
EEEHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
GHFGRAPEADGGFSWERTDLTDALLKAV
CCCCCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SRMNYVFTSESVSEGHPDKLCDRVSDAVLDTFLAEEPTARVACETFATTGRVVVGGEVG
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEECCCCC
LSDPQKLDEFMERVDGIVRDCVKDIGYEQQEFHWRTIEVQNFLHRQSAHIAQGVDKDGAG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEHHHHHHHHHHHHHHHCCCCCCCC
DQGIMFGYACRETPELMPAPIQYSHAILRRLAEVRKSGQEPDLRPDAKSQLSLRYENGKP
CCCEEEEEHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCC
VEVRSIVLSTQHAHEEQTSDDIRAIVEPYIREVLPEGWITEATEWWVNPTGTFVIGGPDG
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEECCCCEEEEECCCC
DAGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYAARYLAKNVVAAGLAERCTLQ
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
VSYAIGVAKPLSIYVDTHGTGQVDAAQIEKAVADCMDLTPRGIREHLNLCRPIYARTSAY
EEEHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
GHFGRAPEADGGFSWERTDLTDALLKAV
CCCCCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA