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The map label for this gene is gsiB [H]

Identifier: 77465525

GI number: 77465525

Start: 604763

End: 606370

Strand: Reverse

Name: gsiB [H]

Synonym: RSP_3525

Alternate gene names: 77465525

Gene position: 606370-604763 (Counterclockwise)

Preceding gene: 77465528

Following gene: 77465524

Centisome position: 64.3

GC content: 66.11

Gene sequence:

>1608_bases
ATGAACAAATCGCACCACCTGCTGATGGACGATCTGGTCACGCGGCTGCGGCGCGGACAGCTGTCGCGTCGCGAGTTTCT
GGCCCGCAGTTCGGCGCTGCTGGCGGCCGGCGCCATGAGCGGCCTGCCCGGTGCGGCGCTTGCCCAGCAGGCGGCACCGA
AGGCCGGCGGCTTCATGCGGCTCGGCCTGCACAATGCCTCGCAGAACGACAACCTCGATCCCGGCAGCTGGTCGACGAGT
TGGACCGGCGCCTCGTTCAACGGCGGCGTCTACAACAACCTCGTCGAGATCCTGCCCGACGGCTCGGTCGCGGGCGATCT
GGCCGAGAGCTGGGAGGCGGAGCCCGGCGCGAAGGTCTGGCGCTTCAAGCTGCGCTCGGGCGTGACCTTCCACAACGGCA
AGAGCCTCGAGGCGGAAGACGTGCGCCAGTCGCTCGAGCATCACATGAAGCCGGACTCGACCTCGGGCGCGCGCGCCATC
GTCGAGCAGATCGAGACCATCGACATCGAAGGGTCCGACACCGTCCGCATCACCCTCTCGGAGGGCAATGCCGACCTGCC
CTACCTCCTGTCGGATTATCACCTCTCGATCTATCCGGCGCTGGAGGGCGGCGGGATCGACATGGAGAGCGCCAACGGCA
CCGGCGCCTTCCTCCTCGAGAGCTTCGAGCCGGGCATCGCCACCCGCCTCAAGCGGAACCCGAACTACCACAAGAACAAC
AAGCCCTATCTCGACGAGGTCGAGTTCATCAACATCACCGACGCCACGGCGCGGCTGAACGCGCTGCTGACCGGCGAGGT
CGATTTCATCCAGGATCTCGACATCCGCAACGTGGCGATGGTCGAGCGCAGCGGCGATTTCTCGGTTCAGCGCGTGCCGA
GCCTGCGCCACTTCACCTTCGACATGGACACCCGCGTCGCGCCCTTCGACAATCCCGATGTGCGGCTGGCGCTGAAATAT
GCGCTCGACCGGGATGACGTGATCGAGAAGGTGTTCCTTGGCGAGGCCACGAAGGGGAACGACAACCCGGTCGCCTCGAT
CCAGAAATTCTACCACGACATGCCCGCGCGCGAATACAGCATCGCGAAGGCCAAGGAGCATCTGGCCAAGGCCGGGCTCG
ATCAGGTGAGCGTCGATCTGTCGGTGGCCGAGAATGCGTTTGCGGGCGCCATCGAGGCGGCGACGCTCTACCAGCGCCAT
GCGGCCGAGGCCGGCATCAACATCAACATCGTGCAGGAGGCGGCCGACGGCTACTGGGAGAACGTCTGGCGCAAGAAGCC
CTTCTGCGCGGTCGACTACTTCGGCCGCGCCACCGTCGACTGGCTGTTCTCGACGAGCTATGTCACCGGCGCGCCGTGGA
ATTCGGGCTGGTCGAACGCGCGGTTCGACGAGCTGCACCAGACGGCGCGGGCCGAGACCGACGAGGCCAAGCGCGCCGCC
TGCTACGCCGAAATGCAGGAGATCCTGCGCGACGACGGCAACGTCATCACCGTGGCCTTCGTGAGCTGGCGCAACGCCGT
CTCGAACCGCATCGGCTTCGGCGAGGTCGGCGGGCTGATGCCGCTCGACAACATGCGGATGTGCGAGCGCTGGTGGGTCA
AGGACTGA

Upstream 100 bases:

>100_bases
CGTGATTTCCATAAGGATCACCGGCAGGTCGGAAGGCTGCGCAAGACAGCTGCATCCCGGCGGGACATCCAAGGATAACC
AAACCTGACAGGGTGAACAC

Downstream 100 bases:

>100_bases
GGTCGGCAGGCCCGGTCTCAGACGGCCCGGAGCGCGAGGCGCGCCCCGGGCGACATTCCGACATTTTCGTTCAGGGCGGA
GAAAACCTGATGCGCTACCC

Product: ABC peptide transporter, periplasmic binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MNKSHHLLMDDLVTRLRRGQLSRREFLARSSALLAAGAMSGLPGAALAQQAAPKAGGFMRLGLHNASQNDNLDPGSWSTS
WTGASFNGGVYNNLVEILPDGSVAGDLAESWEAEPGAKVWRFKLRSGVTFHNGKSLEAEDVRQSLEHHMKPDSTSGARAI
VEQIETIDIEGSDTVRITLSEGNADLPYLLSDYHLSIYPALEGGGIDMESANGTGAFLLESFEPGIATRLKRNPNYHKNN
KPYLDEVEFINITDATARLNALLTGEVDFIQDLDIRNVAMVERSGDFSVQRVPSLRHFTFDMDTRVAPFDNPDVRLALKY
ALDRDDVIEKVFLGEATKGNDNPVASIQKFYHDMPAREYSIAKAKEHLAKAGLDQVSVDLSVAENAFAGAIEAATLYQRH
AAEAGININIVQEAADGYWENVWRKKPFCAVDYFGRATVDWLFSTSYVTGAPWNSGWSNARFDELHQTARAETDEAKRAA
CYAEMQEILRDDGNVITVAFVSWRNAVSNRIGFGEVGGLMPLDNMRMCERWWVKD

Sequences:

>Translated_535_residues
MNKSHHLLMDDLVTRLRRGQLSRREFLARSSALLAAGAMSGLPGAALAQQAAPKAGGFMRLGLHNASQNDNLDPGSWSTS
WTGASFNGGVYNNLVEILPDGSVAGDLAESWEAEPGAKVWRFKLRSGVTFHNGKSLEAEDVRQSLEHHMKPDSTSGARAI
VEQIETIDIEGSDTVRITLSEGNADLPYLLSDYHLSIYPALEGGGIDMESANGTGAFLLESFEPGIATRLKRNPNYHKNN
KPYLDEVEFINITDATARLNALLTGEVDFIQDLDIRNVAMVERSGDFSVQRVPSLRHFTFDMDTRVAPFDNPDVRLALKY
ALDRDDVIEKVFLGEATKGNDNPVASIQKFYHDMPAREYSIAKAKEHLAKAGLDQVSVDLSVAENAFAGAIEAATLYQRH
AAEAGININIVQEAADGYWENVWRKKPFCAVDYFGRATVDWLFSTSYVTGAPWNSGWSNARFDELHQTARAETDEAKRAA
CYAEMQEILRDDGNVITVAFVSWRNAVSNRIGFGEVGGLMPLDNMRMCERWWVKD
>Mature_535_residues
MNKSHHLLMDDLVTRLRRGQLSRREFLARSSALLAAGAMSGLPGAALAQQAAPKAGGFMRLGLHNASQNDNLDPGSWSTS
WTGASFNGGVYNNLVEILPDGSVAGDLAESWEAEPGAKVWRFKLRSGVTFHNGKSLEAEDVRQSLEHHMKPDSTSGARAI
VEQIETIDIEGSDTVRITLSEGNADLPYLLSDYHLSIYPALEGGGIDMESANGTGAFLLESFEPGIATRLKRNPNYHKNN
KPYLDEVEFINITDATARLNALLTGEVDFIQDLDIRNVAMVERSGDFSVQRVPSLRHFTFDMDTRVAPFDNPDVRLALKY
ALDRDDVIEKVFLGEATKGNDNPVASIQKFYHDMPAREYSIAKAKEHLAKAGLDQVSVDLSVAENAFAGAIEAATLYQRH
AAEAGININIVQEAADGYWENVWRKKPFCAVDYFGRATVDWLFSTSYVTGAPWNSGWSNARFDELHQTARAETDEAKRAA
CYAEMQEILRDDGNVITVAFVSWRNAVSNRIGFGEVGGLMPLDNMRMCERWWVKD

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=562, Percent_Identity=25.0889679715303, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1787762, Length=513, Percent_Identity=25.3411306042885, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1789966, Length=475, Percent_Identity=24, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1787052, Length=403, Percent_Identity=25.5583126550869, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787551, Length=514, Percent_Identity=22.7626459143969, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789887, Length=294, Percent_Identity=25.5102040816327, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1789397, Length=339, Percent_Identity=25.0737463126844, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59144; Mature: 59144

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKSHHLLMDDLVTRLRRGQLSRREFLARSSALLAAGAMSGLPGAALAQQAAPKAGGFMR
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEE
LGLHNASQNDNLDPGSWSTSWTGASFNGGVYNNLVEILPDGSVAGDLAESWEAEPGAKVW
EEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCEE
RFKLRSGVTFHNGKSLEAEDVRQSLEHHMKPDSTSGARAIVEQIETIDIEGSDTVRITLS
EEEHHCCCEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHEEECCCCCEEEEEEE
EGNADLPYLLSDYHLSIYPALEGGGIDMESANGTGAFLLESFEPGIATRLKRNPNYHKNN
CCCCCCCEEEECCCEEEEEEECCCCCEEECCCCCCEEEEECCCCCHHHHHCCCCCCCCCC
KPYLDEVEFINITDATARLNALLTGEVDFIQDLDIRNVAMVERSGDFSVQRVPSLRHFTF
CCCCCCEEEEEEECHHHHHEEEEECCCHHHHCCCCCCEEEEECCCCCCCEECCCCEEEEE
DMDTRVAPFDNPDVRLALKYALDRDDVIEKVFLGEATKGNDNPVASIQKFYHDMPAREYS
ECCCEECCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHH
IAKAKEHLAKAGLDQVSVDLSVAENAFAGAIEAATLYQRHAAEAGININIVQEAADGYWE
HHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHH
NVWRKKPFCAVDYFGRATVDWLFSTSYVTGAPWNSGWSNARFDELHQTARAETDEAKRAA
HHHCCCCCEEEECCCCCEEHHEEECCEEECCCCCCCCCCCHHHHHHHHHHCCHHHHHHHH
CYAEMQEILRDDGNVITVAFVSWRNAVSNRIGFGEVGGLMPLDNMRMCERWWVKD
HHHHHHHHHHCCCCEEEEEEEHHHHHHHHCCCCHHCCCCCCCCCHHHHHHHCCCC
>Mature Secondary Structure
MNKSHHLLMDDLVTRLRRGQLSRREFLARSSALLAAGAMSGLPGAALAQQAAPKAGGFMR
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEE
LGLHNASQNDNLDPGSWSTSWTGASFNGGVYNNLVEILPDGSVAGDLAESWEAEPGAKVW
EEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCEE
RFKLRSGVTFHNGKSLEAEDVRQSLEHHMKPDSTSGARAIVEQIETIDIEGSDTVRITLS
EEEHHCCCEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHEEECCCCCEEEEEEE
EGNADLPYLLSDYHLSIYPALEGGGIDMESANGTGAFLLESFEPGIATRLKRNPNYHKNN
CCCCCCCEEEECCCEEEEEEECCCCCEEECCCCCCEEEEECCCCCHHHHHCCCCCCCCCC
KPYLDEVEFINITDATARLNALLTGEVDFIQDLDIRNVAMVERSGDFSVQRVPSLRHFTF
CCCCCCEEEEEEECHHHHHEEEEECCCHHHHCCCCCCEEEEECCCCCCCEECCCCEEEEE
DMDTRVAPFDNPDVRLALKYALDRDDVIEKVFLGEATKGNDNPVASIQKFYHDMPAREYS
ECCCEECCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHH
IAKAKEHLAKAGLDQVSVDLSVAENAFAGAIEAATLYQRHAAEAGININIVQEAADGYWE
HHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHH
NVWRKKPFCAVDYFGRATVDWLFSTSYVTGAPWNSGWSNARFDELHQTARAETDEAKRAA
HHHCCCCCEEEECCCCCEEHHEEECCEEECCCCCCCCCCCHHHHHHHHHHCCHHHHHHHH
CYAEMQEILRDDGNVITVAFVSWRNAVSNRIGFGEVGGLMPLDNMRMCERWWVKD
HHHHHHHHHHCCCCEEEEEEEHHHHHHHHCCCCHHCCCCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA