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The map label for this gene is rnfB [H]

Identifier: 77465207

GI number: 77465207

Start: 246833

End: 247399

Strand: Direct

Name: rnfB [H]

Synonym: RSP_3193

Alternate gene names: 77465207

Gene position: 246833-247399 (Clockwise)

Preceding gene: 77465206

Following gene: 77465208

Centisome position: 26.17

GC content: 72.13

Gene sequence:

>567_bases
ATGATCGAAGCCGCCGTCTCCATGTCTGCCCTGGGCCTCGGGCTGGGCCTCCTGCTCGGGGTGGCCGCCCGCAGGTTCCA
TGTCGAGAGCCCGCCCATCGTCGATGCGATCGAGGGCATCCTGCCCGGCACCAACTGCGGCGCCTGCGGCTATCCCGGCT
GCCGGGGCCTCGCCGAGGCCATGTCGGAGGGCGCGGCCCCCGTCACCGCCTGCGCGCCCGGCGGACGCGACGTGGCGCTG
GCGCTGGCGGCCATCGTCGAGACCGACGGCGGCGGCGGGGCGGTTCCGGGCATGGCCGAGGCCGAGCCCACCGTGGCCTT
CATCTTCGAGGACCACTGCACGGGCTGCATGCGCTGCTTCAAGCGCTGTCCCACCGATGCGATCATCGGCGCGAACAGGC
AGATCCACACCGTGGTCACCGACGCCTGCATCGGCTGCAACGCCTGCATCGAGGCCTGCCCGACCGAGGCCATCGTGGCG
CGCGTGAAGCCGAAGACCCTCAAGAGCTGGTACTGGGACAAGCCCCGCACGGCCTTCGAGGCCCGGGGCACGGAGGTGGC
GGCATGA

Upstream 100 bases:

>100_bases
GCGGGCGCGCCCATCGCCTTCATTTCCGCAGGTCTCCTGTCGATGGCCTTCATGGGCTTCGCGGGACTTGCCCCGAACTG
ACCCGGACCGGAGCCCCACC

Downstream 100 bases:

>100_bases
GCTTTCCCCATTTCGCGGCCCTCTTCCGGCCCTCGCAGTTCTTCGGCATCCGCGGCGGCGTCCACCCCGAGACGCGCAAG
CATCTGAGCTCGGAAAGCCC

Product: electron transport complex protein rnfB

Products: NA

Alternate protein names: Nitrogen fixation protein rnfB [H]

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MIEAAVSMSALGLGLGLLLGVAARRFHVESPPIVDAIEGILPGTNCGACGYPGCRGLAEAMSEGAAPVTACAPGGRDVAL
ALAAIVETDGGGGAVPGMAEAEPTVAFIFEDHCTGCMRCFKRCPTDAIIGANRQIHTVVTDACIGCNACIEACPTEAIVA
RVKPKTLKSWYWDKPRTAFEARGTEVAA

Sequences:

>Translated_188_residues
MIEAAVSMSALGLGLGLLLGVAARRFHVESPPIVDAIEGILPGTNCGACGYPGCRGLAEAMSEGAAPVTACAPGGRDVAL
ALAAIVETDGGGGAVPGMAEAEPTVAFIFEDHCTGCMRCFKRCPTDAIIGANRQIHTVVTDACIGCNACIEACPTEAIVA
RVKPKTLKSWYWDKPRTAFEARGTEVAA
>Mature_188_residues
MIEAAVSMSALGLGLGLLLGVAARRFHVESPPIVDAIEGILPGTNCGACGYPGCRGLAEAMSEGAAPVTACAPGGRDVAL
ALAAIVETDGGGGAVPGMAEAEPTVAFIFEDHCTGCMRCFKRCPTDAIIGANRQIHTVVTDACIGCNACIEACPTEAIVA
RVKPKTLKSWYWDKPRTAFEARGTEVAA

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase [H]

COG id: COG2878

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfB

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787915, Length=172, Percent_Identity=45.9302325581395, Blast_Score=153, Evalue=7e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR010207
- InterPro:   IPR016463
- InterPro:   IPR007202 [H]

Pfam domain/function: PF00037 Fer4; PF04060 FeS [H]

EC number: NA

Molecular weight: Translated: 19249; Mature: 19249

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
9.0 %Cys+Met (Translated Protein)
6.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
9.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEAAVSMSALGLGLGLLLGVAARRFHVESPPIVDAIEGILPGTNCGACGYPGCRGLAEA
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHH
MSEGAAPVTACAPGGRDVALALAAIVETDGGGGAVPGMAEAEPTVAFIFEDHCTGCMRCF
HHCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHH
KRCPTDAIIGANRQIHTVVTDACIGCNACIEACPTEAIVARVKPKTLKSWYWDKPRTAFE
HHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHCCCCCCCHHH
ARGTEVAA
CCCCCCCC
>Mature Secondary Structure
MIEAAVSMSALGLGLGLLLGVAARRFHVESPPIVDAIEGILPGTNCGACGYPGCRGLAEA
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHH
MSEGAAPVTACAPGGRDVALALAAIVETDGGGGAVPGMAEAEPTVAFIFEDHCTGCMRCF
HHCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHH
KRCPTDAIIGANRQIHTVVTDACIGCNACIEACPTEAIVARVKPKTLKSWYWDKPRTAFE
HHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHCCCCCCCHHH
ARGTEVAA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA