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The map label for this gene is rnfA

Identifier: 77465206

GI number: 77465206

Start: 246232

End: 246813

Strand: Direct

Name: rnfA

Synonym: RSP_3192

Alternate gene names: 77465206

Gene position: 246232-246813 (Clockwise)

Preceding gene: 77465200

Following gene: 77465207

Centisome position: 26.11

GC content: 65.12

Gene sequence:

>582_bases
ATGGGCGATTTCTTCTTCATCCTGCTCAGCACCGCGCTCGTGAACAACGTGGTTCTGGTCAAGTTTCTGGGGCTCTGTCC
CTTCATGGGCGTGTCGCGCAAGACCGACGCGGCCATCGGCATGGGCCTCGCCACCACCTTCGTGCTGACCCTTGCCGCCG
GCGCGAGCTGGATGGTCGAGGCGCTGATCCTCGAGCCGCTCGATCTCACCTTCCTGCGCATCCTGAGCCTCATCCTCGTG
ATCGCGGCGATCGTGCAGTTCATCGAAGTGGTGATGCGCAAGCTGGCCCCGGGGCTGCACAGGGCGCTCGGGATCTACCT
GCCGCTCATCACGACCAACTGTGCCGTCCTCGGCGTGGCGCTCCTGAACATCCAGGAGGGGCACGGGCTTGCCTCGAGCC
TGCTCTACGGCTTCGGTTCGGCCTCGGGCTTCACCCTCGTCCTCGTGATCTTCGCCGGTATGCGCGAGCGTCTGGCGCAG
CTTTCGGTGCCCGGTCCCTTCGCGGGCGCGCCCATCGCCTTCATTTCCGCAGGTCTCCTGTCGATGGCCTTCATGGGCTT
CGCGGGACTTGCCCCGAACTGA

Upstream 100 bases:

>100_bases
CTTGGCACGGCGGCTGCTTCTTCCGGGTCGTGACAGATGTGCGACAGCGCCGCTCCCCTCCCGCGATCTCCGCGGAGGCG
GGCGTCAGACGGGACAGGCC

Downstream 100 bases:

>100_bases
CCCGGACCGGAGCCCCACCATGATCGAAGCCGCCGTCTCCATGTCTGCCCTGGGCCTCGGGCTGGGCCTCCTGCTCGGGG
TGGCCGCCCGCAGGTTCCAT

Product: Na(+)-translocating NADH-quinone reductase subunit E

Products: NA

Alternate protein names: Nitrogen fixation protein rnfA

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MGDFFFILLSTALVNNVVLVKFLGLCPFMGVSRKTDAAIGMGLATTFVLTLAAGASWMVEALILEPLDLTFLRILSLILV
IAAIVQFIEVVMRKLAPGLHRALGIYLPLITTNCAVLGVALLNIQEGHGLASSLLYGFGSASGFTLVLVIFAGMRERLAQ
LSVPGPFAGAPIAFISAGLLSMAFMGFAGLAPN

Sequences:

>Translated_193_residues
MGDFFFILLSTALVNNVVLVKFLGLCPFMGVSRKTDAAIGMGLATTFVLTLAAGASWMVEALILEPLDLTFLRILSLILV
IAAIVQFIEVVMRKLAPGLHRALGIYLPLITTNCAVLGVALLNIQEGHGLASSLLYGFGSASGFTLVLVIFAGMRERLAQ
LSVPGPFAGAPIAFISAGLLSMAFMGFAGLAPN
>Mature_192_residues
GDFFFILLSTALVNNVVLVKFLGLCPFMGVSRKTDAAIGMGLATTFVLTLAAGASWMVEALILEPLDLTFLRILSLILVI
AAIVQFIEVVMRKLAPGLHRALGIYLPLITTNCAVLGVALLNIQEGHGLASSLLYGFGSASGFTLVLVIFAGMRERLAQL
SVPGPFAGAPIAFISAGLLSMAFMGFAGLAPN

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase

COG id: COG4657

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrDE/rnfAE family

Homologues:

Organism=Escherichia coli, GI1787914, Length=190, Percent_Identity=63.1578947368421, Blast_Score=241, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNFA_RHOS1 (A3PRP3)

Other databases:

- EMBL:   CP000578
- RefSeq:   YP_001045781.1
- STRING:   A3PRP3
- GeneID:   4898487
- GenomeReviews:   CP000578_GR
- KEGG:   rsh:Rsph17029_3931
- eggNOG:   COG4657
- HOGENOM:   HBG624915
- OMA:   VTTRVDT
- ProtClustDB:   PRK05151
- BioCyc:   RSPH349101:RSPH17029_3931-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_00459
- InterPro:   IPR003667
- InterPro:   IPR011293
- PIRSF:   PIRSF006102
- TIGRFAMs:   TIGR01943

Pfam domain/function: PF02508 Rnf-Nqr

EC number: NA

Molecular weight: Translated: 20230; Mature: 20099

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xe9ccf48)-; HASH(0xde4c36c)-; HASH(0xeee70a8)-; HASH(0xef34d7c)-; HASH(0xeee6ebc)-; HASH(0xeee6e5c)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDFFFILLSTALVNNVVLVKFLGLCPFMGVSRKTDAAIGMGLATTFVLTLAAGASWMVE
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
ALILEPLDLTFLRILSLILVIAAIVQFIEVVMRKLAPGLHRALGIYLPLITTNCAVLGVA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
LLNIQEGHGLASSLLYGFGSASGFTLVLVIFAGMRERLAQLSVPGPFAGAPIAFISAGLL
HHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
SMAFMGFAGLAPN
HHHHHHHHCCCCH
>Mature Secondary Structure 
GDFFFILLSTALVNNVVLVKFLGLCPFMGVSRKTDAAIGMGLATTFVLTLAAGASWMVE
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
ALILEPLDLTFLRILSLILVIAAIVQFIEVVMRKLAPGLHRALGIYLPLITTNCAVLGVA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
LLNIQEGHGLASSLLYGFGSASGFTLVLVIFAGMRERLAQLSVPGPFAGAPIAFISAGLL
HHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
SMAFMGFAGLAPN
HHHHHHHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA