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The map label for this gene is potB [H]

Identifier: 77465052

GI number: 77465052

Start: 73363

End: 74214

Strand: Reverse

Name: potB [H]

Synonym: RSP_3039

Alternate gene names: 77465052

Gene position: 74214-73363 (Counterclockwise)

Preceding gene: 77465053

Following gene: 77465051

Centisome position: 7.87

GC content: 66.78

Gene sequence:

>852_bases
ATGCGATCCTCGGTGCGCACCACCCTGTTCCTCGCGCCGGCCACGGCCTGGCTCCTGTGCATGCTGGTGCTGCCGCTGGT
GGTCGTCATCGTCTTCAGCTTCGGCGAAAGGGGGGCCGCGGGCGGCTACGTCCCGGCCTTCACCCTGGACAATTACGCCA
ACCTCGGCTCCCGCTGGGGCGCCTTCCGCAACACGCTCACCCTCGCCCCGCTGGGCACGGTGCTCTGCCTGGTCATCGCC
TGGCCGCTTGCCTATTTCCTGGCGCTGAAGGCCGACCCGCGCTGGCGGACCCTGCTGCTGATCCTCGTGATCGTGCCGTT
CTGGACCTCGATCCTGATCCGCTCCTATGCCTGGATCCAGATCCTCGGCGGCAACGGCCTGCCCTCGATCATCGCGAGCC
TCGGTCTGCCGCGCCCCCGCCTGATCAACACGCCCTTCGCCGTGATGGTGGGCATCGTCTACGGCTATCTGCCGCTGATG
GTCTTCCCGATCTATGTCGCTCTGGAAAAGCTCGACCGGCGGTTGCTGGAGGCCTCGGCGGATCTCGGCAGCCCGCCCTG
GCGCACCTTCCTGCAGGTGACGCTGCCCCTGTCGATGGCGGGCGTGGCGACAGGCTCCATGCTCGTCTTCATCCTGCTGA
TGGGCGAATTCCTGATCCCGCAGATCCTCGGCGGCGGCAAGGTGTTCTTCGTCGGCAACGCCCTGGTGGACCTGTTCCTG
CAATCGAGGAACTGGCCCTTCGGCGCCGCGGTCGCCGCCGTGCTGGTGATCGTGATGCTGATCGCCGTGACGCTCTATCT
GAAGGCCATCAAGCGGGTCTCGGGCGGGCGTGACGATGTCGCCCTGATGTGA

Upstream 100 bases:

>100_bases
TCTCCTGAATGCGCTCGAGTTCGGCGCCGCCGTCACCCTGACCAACCCGGAACGCGCCGAGGTCATGGCGCGCTTCAAAT
CGGCGTAAGGGACGGAGTCG

Downstream 100 bases:

>100_bases
GGCCTTCGATGCGGATCTATGCCATCGCCGTCTACCTGTTCCTCTACATTCCCATCGGCATCATCGCGCTGTTCTCGCTG
AACGCCGGGCGGCACGCCAC

Product: ABC permidine/putrescine transporter, inner membrane subunit

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MRSSVRTTLFLAPATAWLLCMLVLPLVVVIVFSFGERGAAGGYVPAFTLDNYANLGSRWGAFRNTLTLAPLGTVLCLVIA
WPLAYFLALKADPRWRTLLLILVIVPFWTSILIRSYAWIQILGGNGLPSIIASLGLPRPRLINTPFAVMVGIVYGYLPLM
VFPIYVALEKLDRRLLEASADLGSPPWRTFLQVTLPLSMAGVATGSMLVFILLMGEFLIPQILGGGKVFFVGNALVDLFL
QSRNWPFGAAVAAVLVIVMLIAVTLYLKAIKRVSGGRDDVALM

Sequences:

>Translated_283_residues
MRSSVRTTLFLAPATAWLLCMLVLPLVVVIVFSFGERGAAGGYVPAFTLDNYANLGSRWGAFRNTLTLAPLGTVLCLVIA
WPLAYFLALKADPRWRTLLLILVIVPFWTSILIRSYAWIQILGGNGLPSIIASLGLPRPRLINTPFAVMVGIVYGYLPLM
VFPIYVALEKLDRRLLEASADLGSPPWRTFLQVTLPLSMAGVATGSMLVFILLMGEFLIPQILGGGKVFFVGNALVDLFL
QSRNWPFGAAVAAVLVIVMLIAVTLYLKAIKRVSGGRDDVALM
>Mature_283_residues
MRSSVRTTLFLAPATAWLLCMLVLPLVVVIVFSFGERGAAGGYVPAFTLDNYANLGSRWGAFRNTLTLAPLGTVLCLVIA
WPLAYFLALKADPRWRTLLLILVIVPFWTSILIRSYAWIQILGGNGLPSIIASLGLPRPRLINTPFAVMVGIVYGYLPLM
VFPIYVALEKLDRRLLEASADLGSPPWRTFLQVTLPLSMAGVATGSMLVFILLMGEFLIPQILGGGKVFFVGNALVDLFL
QSRNWPFGAAVAAVLVIVMLIAVTLYLKAIKRVSGGRDDVALM

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=258, Percent_Identity=41.0852713178295, Blast_Score=167, Evalue=5e-43,
Organism=Escherichia coli, GI1787080, Length=245, Percent_Identity=39.5918367346939, Blast_Score=167, Evalue=7e-43,
Organism=Escherichia coli, GI1787713, Length=246, Percent_Identity=32.1138211382114, Blast_Score=112, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30825; Mature: 30825

Theoretical pI: Translated: 10.42; Mature: 10.42

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSSVRTTLFLAPATAWLLCMLVLPLVVVIVFSFGERGAAGGYVPAFTLDNYANLGSRWG
CCCCHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHH
AFRNTLTLAPLGTVLCLVIAWPLAYFLALKADPRWRTLLLILVIVPFWTSILIRSYAWIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEE
ILGGNGLPSIIASLGLPRPRLINTPFAVMVGIVYGYLPLMVFPIYVALEKLDRRLLEASA
EECCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DLGSPPWRTFLQVTLPLSMAGVATGSMLVFILLMGEFLIPQILGGGKVFFVGNALVDLFL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHH
QSRNWPFGAAVAAVLVIVMLIAVTLYLKAIKRVSGGRDDVALM
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRSSVRTTLFLAPATAWLLCMLVLPLVVVIVFSFGERGAAGGYVPAFTLDNYANLGSRWG
CCCCHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHH
AFRNTLTLAPLGTVLCLVIAWPLAYFLALKADPRWRTLLLILVIVPFWTSILIRSYAWIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEE
ILGGNGLPSIIASLGLPRPRLINTPFAVMVGIVYGYLPLMVFPIYVALEKLDRRLLEASA
EECCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DLGSPPWRTFLQVTLPLSMAGVATGSMLVFILLMGEFLIPQILGGGKVFFVGNALVDLFL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHH
QSRNWPFGAAVAAVLVIVMLIAVTLYLKAIKRVSGGRDDVALM
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]