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The map label for this gene is potD-B [H]

Identifier: 77465053

GI number: 77465053

Start: 74227

End: 75384

Strand: Reverse

Name: potD-B [H]

Synonym: RSP_3040

Alternate gene names: 77465053

Gene position: 75384-74227 (Counterclockwise)

Preceding gene: 77465054

Following gene: 77465052

Centisome position: 7.99

GC content: 66.58

Gene sequence:

>1158_bases
ATGACGCAGCACCGTGACCACATCCCGGCGAAAGAGTTTCTTCAGCGGCTGGAAAGCTACCGCAAGGGCTCGATCTCGCG
CCGGCACTTCCTGAACGTGACCGGCCTCGGCGCCGCGACCATGGCGATGGCGGGTGCGATGCCGGGCTTCGCCCGCCGCG
CGCAGGCCCAGGGCGCGATCGGCGACCGCGTCGTGATCGCCACCTGGCCGAACTACCACGACCCGGCCGACCTCGACGCC
TTCCGGGCCGCCACCGGCGCCGCGGTCGACGTCAACGTCTTCGGCTCCAACGAGGAGATGCTCGCGAAGCTGCAGGCGGG
CGGCACGGGTTGGGACGTGGTGGTGGCCACGAACTACACGATCTCGACCTATGTCGAGGCGGGGATCATCGAGGAGCTCG
ACCTGTCGCGCATCCCGAATTTCGACAGGGCTTCGACCGACCCGCGCTTCGCCGATCCGGGCGTCATCGACGGCAAGACC
TACGCCATCCCGCGCAACATCGGCACGACCGGCTATTGCATCAACACCGCCGAGATCGACGGCGAGACGCCGACCACCTG
GAAGGAATTCTGGGATCTGGCGCGCGACCGGCTGTCGGGCCGCGGCATAGTGCATGACTATCAGCTGACCGCCATCGGCA
ACGCACTGAAATACTACGGCTATTCCTTCAACTCGGTCGATCCGGCGGAACTCGCGAAGGCGGAGGAGCTGCTGATCGAC
GCCAAGCCGCATCTCTTCGCGATCACCTCGGATTACCAGCCCTCGATGCGTTCCGGCGATGCGGCTCTGTCGATGTGCTG
GACCGGCGACGCGGTGCAGCTTCAGCGCGACATCCCCGAGATTGCCTATGTGCTCGGCCGCGAGGGCGGCGAGCTCTGGT
CGGACTTCTTCACCATCCCCGCCTCGGCCCCGCACAAGGATGCGGCCTATGCGCTGATCAACTTCCTGCTCGAGCCGAAG
ATGGCCGCGCAGGAGGCCATGTTCCACGGCTATCCGACCGGAGACGCCCGGGTCGACGCGATGCTGCCCGCCGAGATGCG
CGACAGCCCGATCCTGTTCCCGGCTGCGGATCTCCTGAATGCGCTCGAGTTCGGCGCCGCCGTCACCCTGACCAACCCGG
AACGCGCCGAGGTCATGGCGCGCTTCAAATCGGCGTAA

Upstream 100 bases:

>100_bases
CCTGGCGCGACGGCGCCGCTCTGGCGCTCGACGCCGGCTGACTTCAACGACCGGGCCGGCAAGGTCCCGGCGCCACTCCG
ACAACAACAGGGGAATCGAG

Downstream 100 bases:

>100_bases
GGGACGGAGTCGATGCGATCCTCGGTGCGCACCACCCTGTTCCTCGCGCCGGCCACGGCCTGGCTCCTGTGCATGCTGGT
GCTGCCGCTGGTGGTCGTCA

Product: ABC permidine/putrescine transporter, periplasmic substrate-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MTQHRDHIPAKEFLQRLESYRKGSISRRHFLNVTGLGAATMAMAGAMPGFARRAQAQGAIGDRVVIATWPNYHDPADLDA
FRAATGAAVDVNVFGSNEEMLAKLQAGGTGWDVVVATNYTISTYVEAGIIEELDLSRIPNFDRASTDPRFADPGVIDGKT
YAIPRNIGTTGYCINTAEIDGETPTTWKEFWDLARDRLSGRGIVHDYQLTAIGNALKYYGYSFNSVDPAELAKAEELLID
AKPHLFAITSDYQPSMRSGDAALSMCWTGDAVQLQRDIPEIAYVLGREGGELWSDFFTIPASAPHKDAAYALINFLLEPK
MAAQEAMFHGYPTGDARVDAMLPAEMRDSPILFPAADLLNALEFGAAVTLTNPERAEVMARFKSA

Sequences:

>Translated_385_residues
MTQHRDHIPAKEFLQRLESYRKGSISRRHFLNVTGLGAATMAMAGAMPGFARRAQAQGAIGDRVVIATWPNYHDPADLDA
FRAATGAAVDVNVFGSNEEMLAKLQAGGTGWDVVVATNYTISTYVEAGIIEELDLSRIPNFDRASTDPRFADPGVIDGKT
YAIPRNIGTTGYCINTAEIDGETPTTWKEFWDLARDRLSGRGIVHDYQLTAIGNALKYYGYSFNSVDPAELAKAEELLID
AKPHLFAITSDYQPSMRSGDAALSMCWTGDAVQLQRDIPEIAYVLGREGGELWSDFFTIPASAPHKDAAYALINFLLEPK
MAAQEAMFHGYPTGDARVDAMLPAEMRDSPILFPAADLLNALEFGAAVTLTNPERAEVMARFKSA
>Mature_384_residues
TQHRDHIPAKEFLQRLESYRKGSISRRHFLNVTGLGAATMAMAGAMPGFARRAQAQGAIGDRVVIATWPNYHDPADLDAF
RAATGAAVDVNVFGSNEEMLAKLQAGGTGWDVVVATNYTISTYVEAGIIEELDLSRIPNFDRASTDPRFADPGVIDGKTY
AIPRNIGTTGYCINTAEIDGETPTTWKEFWDLARDRLSGRGIVHDYQLTAIGNALKYYGYSFNSVDPAELAKAEELLIDA
KPHLFAITSDYQPSMRSGDAALSMCWTGDAVQLQRDIPEIAYVLGREGGELWSDFFTIPASAPHKDAAYALINFLLEPKM
AAQEAMFHGYPTGDARVDAMLPAEMRDSPILFPAADLLNALEFGAAVTLTNPERAEVMARFKSA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=310, Percent_Identity=31.9354838709677, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1787078, Length=316, Percent_Identity=28.4810126582279, Blast_Score=111, Evalue=6e-26,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 42024; Mature: 41893

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQHRDHIPAKEFLQRLESYRKGSISRRHFLNVTGLGAATMAMAGAMPGFARRAQAQGAI
CCCCCCCCCHHHHHHHHHHHHCCCCCHHHEEEEECCCHHHHHHHHCCCHHHHHHHHCCCC
GDRVVIATWPNYHDPADLDAFRAATGAAVDVNVFGSNEEMLAKLQAGGTGWDVVVATNYT
CCEEEEEECCCCCCCCCHHHHHHCCCCEEEEEEECCCHHHHHHHHCCCCCEEEEEEECCE
ISTYVEAGIIEELDLSRIPNFDRASTDPRFADPGVIDGKTYAIPRNIGTTGYCINTAEID
EEEHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCEECCCEEEECCCCCCCEEEEEEEEEC
GETPTTWKEFWDLARDRLSGRGIVHDYQLTAIGNALKYYGYSFNSVDPAELAKAEELLID
CCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHEEE
AKPHLFAITSDYQPSMRSGDAALSMCWTGDAVQLQRDIPEIAYVLGREGGELWSDFFTIP
CCCCEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHEECC
ASAPHKDAAYALINFLLEPKMAAQEAMFHGYPTGDARVDAMLPAEMRDSPILFPAADLLN
CCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEHHHHHH
ALEFGAAVTLTNPERAEVMARFKSA
HHHHCCEEEECCCHHHHHHHHHHCC
>Mature Secondary Structure 
TQHRDHIPAKEFLQRLESYRKGSISRRHFLNVTGLGAATMAMAGAMPGFARRAQAQGAI
CCCCCCCCHHHHHHHHHHHHCCCCCHHHEEEEECCCHHHHHHHHCCCHHHHHHHHCCCC
GDRVVIATWPNYHDPADLDAFRAATGAAVDVNVFGSNEEMLAKLQAGGTGWDVVVATNYT
CCEEEEEECCCCCCCCCHHHHHHCCCCEEEEEEECCCHHHHHHHHCCCCCEEEEEEECCE
ISTYVEAGIIEELDLSRIPNFDRASTDPRFADPGVIDGKTYAIPRNIGTTGYCINTAEID
EEEHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCEECCCEEEECCCCCCCEEEEEEEEEC
GETPTTWKEFWDLARDRLSGRGIVHDYQLTAIGNALKYYGYSFNSVDPAELAKAEELLID
CCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHEEE
AKPHLFAITSDYQPSMRSGDAALSMCWTGDAVQLQRDIPEIAYVLGREGGELWSDFFTIP
CCCCEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHEECC
ASAPHKDAAYALINFLLEPKMAAQEAMFHGYPTGDARVDAMLPAEMRDSPILFPAADLLN
CCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEHHHHHH
ALEFGAAVTLTNPERAEVMARFKSA
HHHHCCEEEECCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]