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The map label for this gene is pilT [H]

Identifier: 77461539

GI number: 77461539

Start: 5982168

End: 5983202

Strand: Reverse

Name: pilT [H]

Synonym: Pfl01_5318

Alternate gene names: 77461539

Gene position: 5983202-5982168 (Counterclockwise)

Preceding gene: 77461550

Following gene: 77461537

Centisome position: 92.93

GC content: 61.06

Gene sequence:

>1035_bases
ATGGATATCACTGAACTGCTGGCCTTCAGCGCCAAACAGGGCGCTTCCGACCTGCACCTGTCGGCCGGTCTGCCGCCGAT
GATCCGCGTGGATGGCGATGTGCGGCGGATCAATCTGCCGGCGCTGGATCACAAACAGGTGCACGAGCTGATCTACGACA
TCATGAACGACACCCAGCGGGTGGACTTCGAGAAACATCTGGAAACCGACTTTTCCTTCGAAGTCCCCGGCGTGGCGCGC
TTCCGGGTCAATGCCTTCAACCAGAACCGTGGCGCTGGCGCGGTGTTCCGGACCATTCCGTCGAAAGTGCTGAGCATGGA
AGACCTCGCCATGGGCGACGTCTTTCGCAAGATCACCGATGCGCCACGCGGGCTGGTGCTGGTGACCGGGCCGACCGGCT
CCGGCAAGTCAACGACCCTGGCGGCGATGATCGATTACCTCAACACCCATCGCCATCACCACATCCTGACCATTGAAGAT
CCGATCGAATTTGTCCACGAATCGCGCAAATGCCTGATCAATCAGCGCGAAGTCCATCGCGATACCCGCAGTTTCGCCAC
CGCATTGCGTTCGGCATTGCGCGAAGACCCGGACGTGATCCTGGTGGGCGAGATGCGCGATCTGGAAACCATTCGCCTGG
CGCTGACGGCGGCCGAGACCGGGCACCTGGTGTTCGGCACCTTGCACACTACTTCGGCAGCCAAGACCATCGACCGTGTC
GTGGACGTGTTCCCCGGGGACGAAAAATCCATGGTGCGCTCGATGCTGTCGGAGTCGTTGCTGGCGGTGGTGTCGCAGAC
GCTGATCAAGAAGATCGGCGGTGGTCGGGTGGCGGCGCACGAGATCATGCTGGGCACTTCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATACAGACCGGCGGCTCGCTGGGGATGCAGACACTCGACATGTGCCTG
AAGGATCTGGTGACCAAAGGCTTGATCAGCCGCGAACATGCGCGGGAGAAGGCGCGCACGCCGGATAACTTTTAG

Upstream 100 bases:

>100_bases
ATACGCGAACTAACCTGGAGAATGTTGTCTGCTATCGTGGACATCAAGAGGCGCCAGCGGTCTGAAGGTTCGCGGCATTC
TACTGGAATTGAGGGACGCT

Downstream 100 bases:

>100_bases
GCAAAAAAAACGCAGCCCGTGGGCTGCGTTTTTTTACCTGAACAATCAGCGCTGAACGACGCGCATCTGCCCCTGTTCTT
TCGGCAATACGCGTTTGGCT

Product: pilus retraction protein PilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDTQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLAMGDVFRKITDAPRGLVLVTGPTGSGKSTTLAAMIDYLNTHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KDLVTKGLISREHAREKARTPDNF

Sequences:

>Translated_344_residues
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDTQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLAMGDVFRKITDAPRGLVLVTGPTGSGKSTTLAAMIDYLNTHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KDLVTKGLISREHAREKARTPDNF
>Mature_344_residues
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDTQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLAMGDVFRKITDAPRGLVLVTGPTGSGKSTTLAAMIDYLNTHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KDLVTKGLISREHAREKARTPDNF

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=327, Percent_Identity=46.4831804281346, Blast_Score=285, Evalue=2e-78,
Organism=Escherichia coli, GI1789723, Length=284, Percent_Identity=37.3239436619718, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1786296, Length=212, Percent_Identity=38.6792452830189, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 38072; Mature: 38072

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDTQR
CCHHHHHHHHHHCCCCCEEECCCCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHCCHHH
VDFEKHLETDFSFEVPGVARFRVNAFNQNRGAGAVFRTIPSKVLSMEDLAMGDVFRKITD
CCHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
APRGLVLVTGPTGSGKSTTLAAMIDYLNTHRHHHILTIEDPIEFVHESRKCLINQREVHR
CCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
DTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHEEEECCCCCEEEEEEHHHHHHHHHHHH
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQ
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MYSAIQTGGSLGMQTLDMCLKDLVTKGLISREHAREKARTPDNF
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDTQR
CCHHHHHHHHHHCCCCCEEECCCCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHCCHHH
VDFEKHLETDFSFEVPGVARFRVNAFNQNRGAGAVFRTIPSKVLSMEDLAMGDVFRKITD
CCHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
APRGLVLVTGPTGSGKSTTLAAMIDYLNTHRHHHILTIEDPIEFVHESRKCLINQREVHR
CCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
DTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHEEEECCCCCEEEEEEHHHHHHHHHHHH
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQ
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MYSAIQTGGSLGMQTLDMCLKDLVTKGLISREHAREKARTPDNF
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]