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The map label for this gene is trmB [H]

Identifier: 77461550

GI number: 77461550

Start: 5990177

End: 5990902

Strand: Reverse

Name: trmB [H]

Synonym: Pfl01_5329

Alternate gene names: 77461550

Gene position: 5990902-5990177 (Counterclockwise)

Preceding gene: 77461551

Following gene: 77461539

Centisome position: 93.05

GC content: 62.67

Gene sequence:

>726_bases
ATGACTGAATCGAACGACACGCCAATCCAGACGGAAGAAGGCGACGAGCGCCAACACCGCCGCATCAAGAGTTTCGTGAT
GCGCGCCGGGCGCATGACCGAAGGCCAGCAGCGTGGCCTGGATCAGGGCGCGCCGAAGTTCGTCCTGCCGCTGGCCGATG
CGCCGGTGGATTTCGACCAGGTGTTCGGCCGTTCCGCGCCGCGTTCGCTGGAGATCGGTTTCGGTATGGGCCACTCGCTG
CTGGAAATGGCAGCGGCTGCGCCGGAACAGGATTTCATCGGTGTCGAGGTTCACCGTCCGGGTGTCGGCGCGCTGCTCAA
TGGCGTGCTGACTCAGGGCCTGACCAACCTGCGGGTCTACGATTGCGACGCGATCGAAGTGCTCAACCGCTGCATTGCCG
ACAACAGCCTCGATCGCCTGATGCTGTTCTTCCCGGATCCGTGGCACAAGAGCCGTCACCACAAGCGCCGTATCGTTCAG
GCGTCTTTCGCTGAGCTGGTGCGCAGCAAGCTGAAGGTTGGCGGCATTCTGCACATGGCCACCGACTGGGAGCCGTACGC
CGAATACATGCTGGAAGTGATGAACGTCGCCCCGGGCTATCGCAACCTCGCCGAAGACGGCAAGTGCGTGCCACGCCCGG
CCGAGCGTCCGATCACCAAGTTCGAACGCCGTGGCGAACGTCTTGGGCATGGCGTGTGGGATCTGAAGTTCGAAAAGCTC
GCTTAA

Upstream 100 bases:

>100_bases
CGTCGCGGGCCGCCTGGCCTACCTCGCCGGCCGCATGCCGAAAAAACTCTATGCCAGCGCCTCTTCGCCGCTGGATGGTC
TGATCAAGTAAGAGCTATTG

Downstream 100 bases:

>100_bases
ACGTTACGCAATAAAAAACTGTGGGAGCCAATGGCTCCCACAGTTGTTTTGGGTAGTAGCTGAAAATCAGCGGCGATCCG
CGACCACGCCAATCAACACA

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MTESNDTPIQTEEGDERQHRRIKSFVMRAGRMTEGQQRGLDQGAPKFVLPLADAPVDFDQVFGRSAPRSLEIGFGMGHSL
LEMAAAAPEQDFIGVEVHRPGVGALLNGVLTQGLTNLRVYDCDAIEVLNRCIADNSLDRLMLFFPDPWHKSRHHKRRIVQ
ASFAELVRSKLKVGGILHMATDWEPYAEYMLEVMNVAPGYRNLAEDGKCVPRPAERPITKFERRGERLGHGVWDLKFEKL
A

Sequences:

>Translated_241_residues
MTESNDTPIQTEEGDERQHRRIKSFVMRAGRMTEGQQRGLDQGAPKFVLPLADAPVDFDQVFGRSAPRSLEIGFGMGHSL
LEMAAAAPEQDFIGVEVHRPGVGALLNGVLTQGLTNLRVYDCDAIEVLNRCIADNSLDRLMLFFPDPWHKSRHHKRRIVQ
ASFAELVRSKLKVGGILHMATDWEPYAEYMLEVMNVAPGYRNLAEDGKCVPRPAERPITKFERRGERLGHGVWDLKFEKL
A
>Mature_240_residues
TESNDTPIQTEEGDERQHRRIKSFVMRAGRMTEGQQRGLDQGAPKFVLPLADAPVDFDQVFGRSAPRSLEIGFGMGHSLL
EMAAAAPEQDFIGVEVHRPGVGALLNGVLTQGLTNLRVYDCDAIEVLNRCIADNSLDRLMLFFPDPWHKSRHHKRRIVQA
SFAELVRSKLKVGGILHMATDWEPYAEYMLEVMNVAPGYRNLAEDGKCVPRPAERPITKFERRGERLGHGVWDLKFEKLA

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=236, Percent_Identity=58.8983050847458, Blast_Score=287, Evalue=5e-79,
Organism=Saccharomyces cerevisiae, GI6320000, Length=168, Percent_Identity=27.3809523809524, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 27163; Mature: 27032

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTESNDTPIQTEEGDERQHRRIKSFVMRAGRMTEGQQRGLDQGAPKFVLPLADAPVDFDQ
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCEEEEECCCCCCCHHH
VFGRSAPRSLEIGFGMGHSLLEMAAAAPEQDFIGVEVHRPGVGALLNGVLTQGLTNLRVY
HHCCCCCCEEEECCCCCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEE
DCDAIEVLNRCIADNSLDRLMLFFPDPWHKSRHHKRRIVQASFAELVRSKLKVGGILHMA
CCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEC
TDWEPYAEYMLEVMNVAPGYRNLAEDGKCVPRPAERPITKFERRGERLGHGVWDLKFEKL
CCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHC
A
C
>Mature Secondary Structure 
TESNDTPIQTEEGDERQHRRIKSFVMRAGRMTEGQQRGLDQGAPKFVLPLADAPVDFDQ
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCEEEEECCCCCCCHHH
VFGRSAPRSLEIGFGMGHSLLEMAAAAPEQDFIGVEVHRPGVGALLNGVLTQGLTNLRVY
HHCCCCCCEEEECCCCCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEE
DCDAIEVLNRCIADNSLDRLMLFFPDPWHKSRHHKRRIVQASFAELVRSKLKVGGILHMA
CCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEC
TDWEPYAEYMLEVMNVAPGYRNLAEDGKCVPRPAERPITKFERRGERLGHGVWDLKFEKL
CCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA