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The map label for this gene is pilG [H]

Identifier: 77461529

GI number: 77461529

Start: 5973978

End: 5974382

Strand: Reverse

Name: pilG [H]

Synonym: Pfl01_5308

Alternate gene names: 77461529

Gene position: 5974382-5973978 (Counterclockwise)

Preceding gene: 77461537

Following gene: 77461528

Centisome position: 92.79

GC content: 55.56

Gene sequence:

>405_bases
ATGGAACAGCAGTCCAGCGCCTTGAAGGTCATGGTGATCGACGACTCGAAAACGATTCGTCGCACCGCCGAGACCCTGTT
GAAAAATGCAGGGTGCGAAGTCATCACCGCAATCGACGGTTTTGACGCCCTGGCGAAGATCGCCGATCACCATCCCGGAA
TCATCTTTGTCGACATCATGATGCCGCGTCTGGATGGTTATCAGACCTGCGCTTTAATCAAGAACAACAACGCGTTCAAG
GGCACGCCGGTGATCATGCTGTCGTCCCGGGACGGGCTGTTCGACAAGGCCAAGGGGCGGATTGTCGGTTCCGATCAATT
TTTGACCAAGCCTTTCAGCAAGGAAGAACTGCTCGACGCGATTTCGGCCCATGTTCCGGGCTTCGCTGCCGCTTTGCCGC
AGTAA

Upstream 100 bases:

>100_bases
AGCTGAGAACGCCCCCTGCCCTTGAGCATCAAGGGTTTCCAGCCATCAGTCATCGCAAAACGGACAAATTGATTCGCAAA
GGCGACGGTAGAGCCCCCTT

Downstream 100 bases:

>100_bases
GACACGCACAGAGACGCTCGGCCAGCGGGCCCGGTGTCGACAAGAAAAATGGGGAAGACCATGGCACGTATCCTGATCGT
CGACGATTCGCCGACCGAAA

Product: response regulator receiver domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 134; Mature: 134

Protein sequence:

>134_residues
MEQQSSALKVMVIDDSKTIRRTAETLLKNAGCEVITAIDGFDALAKIADHHPGIIFVDIMMPRLDGYQTCALIKNNNAFK
GTPVIMLSSRDGLFDKAKGRIVGSDQFLTKPFSKEELLDAISAHVPGFAAALPQ

Sequences:

>Translated_134_residues
MEQQSSALKVMVIDDSKTIRRTAETLLKNAGCEVITAIDGFDALAKIADHHPGIIFVDIMMPRLDGYQTCALIKNNNAFK
GTPVIMLSSRDGLFDKAKGRIVGSDQFLTKPFSKEELLDAISAHVPGFAAALPQ
>Mature_134_residues
MEQQSSALKVMVIDDSKTIRRTAETLLKNAGCEVITAIDGFDALAKIADHHPGIIFVDIMMPRLDGYQTCALIKNNNAFK
GTPVIMLSSRDGLFDKAKGRIVGSDQFLTKPFSKEELLDAISAHVPGFAAALPQ

Specific function: Is involved in pilus biosynthesis and twitching motility. May act as a single-domain response regulator whose function may be to receive a certain environmental signal(s) and then transduce that signal to the pilus assembly machinery via specific protein-

COG id: COG0784

COG function: function code T; FOG: CheY-like receiver

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=117, Percent_Identity=30.7692307692308, Blast_Score=69, Evalue=9e-14,
Organism=Escherichia coli, GI87082012, Length=118, Percent_Identity=32.2033898305085, Blast_Score=69, Evalue=1e-13,
Organism=Escherichia coli, GI1787375, Length=116, Percent_Identity=28.448275862069, Blast_Score=62, Evalue=1e-11,
Organism=Escherichia coli, GI1787487, Length=111, Percent_Identity=27.9279279279279, Blast_Score=60, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 14533; Mature: 14533

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQQSSALKVMVIDDSKTIRRTAETLLKNAGCEVITAIDGFDALAKIADHHPGIIFVDIM
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCCEEEEEEE
MPRLDGYQTCALIKNNNAFKGTPVIMLSSRDGLFDKAKGRIVGSDQFLTKPFSKEELLDA
CCCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCEEECCCCCCCCCCCHHHHHHH
ISAHVPGFAAALPQ
HHHCCCCCHHCCCC
>Mature Secondary Structure
MEQQSSALKVMVIDDSKTIRRTAETLLKNAGCEVITAIDGFDALAKIADHHPGIIFVDIM
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCCEEEEEEE
MPRLDGYQTCALIKNNNAFKGTPVIMLSSRDGLFDKAKGRIVGSDQFLTKPFSKEELLDA
CCCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCEEECCCCCCCCCCCHHHHHHH
ISAHVPGFAAALPQ
HHHCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8104179; 10984043 [H]