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The map label for this gene is hsrA [H]

Identifier: 77461468

GI number: 77461468

Start: 5906790

End: 5908217

Strand: Direct

Name: hsrA [H]

Synonym: Pfl01_5247

Alternate gene names: 77461468

Gene position: 5906790-5908217 (Clockwise)

Preceding gene: 77461467

Following gene: 77461469

Centisome position: 91.74

GC content: 65.34

Gene sequence:

>1428_bases
ATGCCCAACCGCCCGCCTCTCGACGCCATCACCGCCCGCTGGTTGCCATGGGTCGTCGCCATCGCTTTCTTCATGCAGTC
CCTCGACGGGACCATCCTCAACACCGCCCTGCCAGCCATGGCCAGGGATCTGGCCGAAGACCCGCTGCGCATGCAGGGCG
TGATCATCGCCTACATGCTCACCGTGGCCCTGCTGATTCCCGCCTCGGGCTGGATCGCCGACCGCTTCGGCACCAAGAAA
ATCTTCTTCGGCGCGATCCTGCTGTTCAGCCTCGGCTCGCTGCTGTGTGCCTTGTCGAGCAGCCTGAGCATGCTGATCGG
CGCGCGAGTGATCCAGGGCCTCGGCGGTGCGCTGATGCTGCCGGTCGGACGCCTGGTGGTGCTGCGCGCCTACCCGCGTT
CGGAACTGGTGCGGATCATGGGCTTCATCACCATTCCCGGCCTGCTCGGCCCGCTGATCGGTCCGACCATGGGCGGCTGG
ATGGTCGAATACCTGACGTGGCACTGGATCTTCCTGATCAACCTGCCGGTCGGCGTCATCGGTTGCTACGCGGTGTGGAA
ATTCATCCCCGACCTGCGCGGTACCGAGCGCACGCGTTTCGATAGCCTCGGTTTCCTGCTGTTCGGCGCGGCGATGGTGC
TGATCACCATCGCCATGGAAGGCCTCGGCGAACTGCACCTGCCGCACCTGCGGGTGATGCTGTTGCTGTTCGGCGGCATG
GCATGTCTGGCGGCGTACTGGCTGCGCGCCGGGCACATTGAAAACCCGCTGTTCGCGCCGTCGCTGTTCAAGACCCGCAC
CTTCGCGGTCGGCATCCTCGGCAACCTGTTCGCCCGACTGGGCAGCGGCGCCCTGCCGTTTCTGGTGCCGTTGCTGCTGC
AAGTGGCGCTGGGTTATTCGCCGTCCCAGGCCGGGATGAGCATGCTGCCGCTGGCGGCGGCCGCGATGGTTGCCAAGTGG
GGCGCGCGGCCACTGATCGAGCGCTTGGGCTATCGCATCGTGCTGACCGGCAATACCCTGCTTCTGGGAATCATGCTGGC
GAGCATGGGGCTGGTCAGCGAGCAGACGCCGTACTGGCTGCTGCTGTGCCTGCTGGCGGTTCTCGGGGCGATCAACTCGC
TGCAATTCACCGCGATGAACACCGTGACCCTGATCGACCTCGACGACGCCAGCGCCAGCAGCGGCAACAGCCTGCTGTCG
GTGGTGGCGCAGTTGTCGCTGAGCCTCGGCGTGGCGTGCGCCGGTGCGCTGCTTGGCGGCTTTACGGCGGAAGTCGGCAA
CGACGGCGTTGAGACCGTGTTGGGCGCATTTCAGCTCACGTTCGTGACCGTGGGGATCATGGCGATGCTGGCGGCGACGA
TTTTCTCGCAGCTATCGAAAGAGGACGGACGGCGCGCCAAACGCCGGGAAGAACATATCGAGCATTGA

Upstream 100 bases:

>100_bases
CGTGACGCTCCGCGTCACTTTACAGCGGACGCAGAGCGTCCATGGCGGCATTCCCACGCGGAGCGTGGGAACGATCATCC
AACAACCAACAAGAGGTTCC

Downstream 100 bases:

>100_bases
CCGGGGTCTGCGTGGCGAGGGAACCGGCCCTCGTCACAGGCTTTGCGTTCGTCCAGAACTTTCGAGGAAAGTGGCCAGAG
GCTGCTACACTGCGCGACAT

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MPNRPPLDAITARWLPWVVAIAFFMQSLDGTILNTALPAMARDLAEDPLRMQGVIIAYMLTVALLIPASGWIADRFGTKK
IFFGAILLFSLGSLLCALSSSLSMLIGARVIQGLGGALMLPVGRLVVLRAYPRSELVRIMGFITIPGLLGPLIGPTMGGW
MVEYLTWHWIFLINLPVGVIGCYAVWKFIPDLRGTERTRFDSLGFLLFGAAMVLITIAMEGLGELHLPHLRVMLLLFGGM
ACLAAYWLRAGHIENPLFAPSLFKTRTFAVGILGNLFARLGSGALPFLVPLLLQVALGYSPSQAGMSMLPLAAAAMVAKW
GARPLIERLGYRIVLTGNTLLLGIMLASMGLVSEQTPYWLLLCLLAVLGAINSLQFTAMNTVTLIDLDDASASSGNSLLS
VVAQLSLSLGVACAGALLGGFTAEVGNDGVETVLGAFQLTFVTVGIMAMLAATIFSQLSKEDGRRAKRREEHIEH

Sequences:

>Translated_475_residues
MPNRPPLDAITARWLPWVVAIAFFMQSLDGTILNTALPAMARDLAEDPLRMQGVIIAYMLTVALLIPASGWIADRFGTKK
IFFGAILLFSLGSLLCALSSSLSMLIGARVIQGLGGALMLPVGRLVVLRAYPRSELVRIMGFITIPGLLGPLIGPTMGGW
MVEYLTWHWIFLINLPVGVIGCYAVWKFIPDLRGTERTRFDSLGFLLFGAAMVLITIAMEGLGELHLPHLRVMLLLFGGM
ACLAAYWLRAGHIENPLFAPSLFKTRTFAVGILGNLFARLGSGALPFLVPLLLQVALGYSPSQAGMSMLPLAAAAMVAKW
GARPLIERLGYRIVLTGNTLLLGIMLASMGLVSEQTPYWLLLCLLAVLGAINSLQFTAMNTVTLIDLDDASASSGNSLLS
VVAQLSLSLGVACAGALLGGFTAEVGNDGVETVLGAFQLTFVTVGIMAMLAATIFSQLSKEDGRRAKRREEHIEH
>Mature_474_residues
PNRPPLDAITARWLPWVVAIAFFMQSLDGTILNTALPAMARDLAEDPLRMQGVIIAYMLTVALLIPASGWIADRFGTKKI
FFGAILLFSLGSLLCALSSSLSMLIGARVIQGLGGALMLPVGRLVVLRAYPRSELVRIMGFITIPGLLGPLIGPTMGGWM
VEYLTWHWIFLINLPVGVIGCYAVWKFIPDLRGTERTRFDSLGFLLFGAAMVLITIAMEGLGELHLPHLRVMLLLFGGMA
CLAAYWLRAGHIENPLFAPSLFKTRTFAVGILGNLFARLGSGALPFLVPLLLQVALGYSPSQAGMSMLPLAAAAMVAKWG
ARPLIERLGYRIVLTGNTLLLGIMLASMGLVSEQTPYWLLLCLLAVLGAINSLQFTAMNTVTLIDLDDASASSGNSLLSV
VAQLSLSLGVACAGALLGGFTAEVGNDGVETVLGAFQLTFVTVGIMAMLAATIFSQLSKEDGRRAKRREEHIEH

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=458, Percent_Identity=49.3449781659389, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI1788392, Length=466, Percent_Identity=44.6351931330472, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1789042, Length=337, Percent_Identity=30.2670623145401, Blast_Score=131, Evalue=9e-32,
Organism=Escherichia coli, GI1788710, Length=324, Percent_Identity=30.5555555555556, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI87081983, Length=313, Percent_Identity=30.0319488817891, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI1788509, Length=136, Percent_Identity=32.3529411764706, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI87082312, Length=183, Percent_Identity=31.1475409836066, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI87082320, Length=153, Percent_Identity=27.4509803921569, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6321663, Length=121, Percent_Identity=35.5371900826446, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6325455, Length=129, Percent_Identity=34.1085271317829, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6322958, Length=160, Percent_Identity=31.25, Blast_Score=67, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6319770, Length=334, Percent_Identity=22.4550898203593, Blast_Score=66, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50956; Mature: 50825

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNRPPLDAITARWLPWVVAIAFFMQSLDGTILNTALPAMARDLAEDPLRMQGVIIAYML
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
TVALLIPASGWIADRFGTKKIFFGAILLFSLGSLLCALSSSLSMLIGARVIQGLGGALML
HHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
PVGRLVVLRAYPRSELVRIMGFITIPGLLGPLIGPTMGGWMVEYLTWHWIFLINLPVGVI
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
GCYAVWKFIPDLRGTERTRFDSLGFLLFGAAMVLITIAMEGLGELHLPHLRVMLLLFGGM
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
ACLAAYWLRAGHIENPLFAPSLFKTRTFAVGILGNLFARLGSGALPFLVPLLLQVALGYS
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
PSQAGMSMLPLAAAAMVAKWGARPLIERLGYRIVLTGNTLLLGIMLASMGLVSEQTPYWL
CCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCCHHHH
LLCLLAVLGAINSLQFTAMNTVTLIDLDDASASSGNSLLSVVAQLSLSLGVACAGALLGG
HHHHHHHHHHHCCCEEEEECEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FTAEVGNDGVETVLGAFQLTFVTVGIMAMLAATIFSQLSKEDGRRAKRREEHIEH
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PNRPPLDAITARWLPWVVAIAFFMQSLDGTILNTALPAMARDLAEDPLRMQGVIIAYML
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH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HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]