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The map label for this gene is bioH [H]

Identifier: 77461395

GI number: 77461395

Start: 5819299

End: 5820030

Strand: Reverse

Name: bioH [H]

Synonym: Pfl01_5174

Alternate gene names: 77461395

Gene position: 5820030-5819299 (Counterclockwise)

Preceding gene: 77461396

Following gene: 77461394

Centisome position: 90.4

GC content: 65.85

Gene sequence:

>732_bases
ATGCGTGATCGCCTGATTCTGCTACCGGGCTGGGGCCTCGGCGTTTCGCCGCTGGAGCCGCTGGCCGCTGCGTTGCGCGG
ACTCGACGAACACCTGCAGGTCGACATCGAGCCGTTGCCGGAGCTGGACTCCAGCGATCTGCAAGACTGGCTCGACGAGC
TCGATGAGCGCATCTCCGAAGATGCATGGCTCGGTGGCTGGTCACTGGGAGGCATGCTCGCGTCCGAGCTGGCGGCGCGG
CGTGGCGAACATTGTTGCGGCTTGCTGACCCTGGCGAGCAATCCTTCGTTTGTCGCCCATGAACAGTGGCAGAGCGCGAT
GCCCGGCGAGACCTTCGATGCGTTTCTGGCCGGCTGCAGCGCCGACCCGCGCACGACCCTCAAGCGATTCTCTCTGTTGT
GCGCCCAGGGCGCGCAAGATGCGCGCGGCTTGTCGCGGCTGTTGCTCGGCGGCGCGCCGAATACCTCGCCGGCCGCGTTG
ATGGCCGGTCTGGAATTGTTGGCGCAGATCGATACGCGCGCTGCCTTGCAGGCGTTTCGTGGCCCGCAATTGCATCTGTT
CGGCGGGCTGGACGGGTTGGTGCCGGCGGAGGCCGCGGGCGAGCTGTTCGCGTTGCTGGCGGATGTCGAGGTCGGCCTGA
TCGAGCAGGCCAGCCATGCGTTTCTTCTGGAGGATCCCCACGGTGTGGCGGGTGCAATCCAGGCGTTTTTAAGTGAGTGC
GGTGATGACTGA

Upstream 100 bases:

>100_bases
TTGCGGGTGACGCTGACCGCCGCTCACAGCGAGGCACAGGTGCAGCTATTGTTGGAAGGACTGGCCGATTGCTTTGCGCA
ACTCAAGTCGGAGCCAAGCC

Downstream 100 bases:

>100_bases
TTTGTCTGTCGCGGCGTTGCCCGGCGGCTTGCCTGACAAGCGCCAGGTCGCGGCTTCTTTTTCTCGCGCAGCGGCCAGCT
ACGACAGCGTCGCCGAGTTG

Product: alpha/beta hydrolase fold protein

Products: NA

Alternate protein names: Biotin synthesis protein BioH [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MRDRLILLPGWGLGVSPLEPLAAALRGLDEHLQVDIEPLPELDSSDLQDWLDELDERISEDAWLGGWSLGGMLASELAAR
RGEHCCGLLTLASNPSFVAHEQWQSAMPGETFDAFLAGCSADPRTTLKRFSLLCAQGAQDARGLSRLLLGGAPNTSPAAL
MAGLELLAQIDTRAALQAFRGPQLHLFGGLDGLVPAEAAGELFALLADVEVGLIEQASHAFLLEDPHGVAGAIQAFLSEC
GDD

Sequences:

>Translated_243_residues
MRDRLILLPGWGLGVSPLEPLAAALRGLDEHLQVDIEPLPELDSSDLQDWLDELDERISEDAWLGGWSLGGMLASELAAR
RGEHCCGLLTLASNPSFVAHEQWQSAMPGETFDAFLAGCSADPRTTLKRFSLLCAQGAQDARGLSRLLLGGAPNTSPAAL
MAGLELLAQIDTRAALQAFRGPQLHLFGGLDGLVPAEAAGELFALLADVEVGLIEQASHAFLLEDPHGVAGAIQAFLSEC
GDD
>Mature_243_residues
MRDRLILLPGWGLGVSPLEPLAAALRGLDEHLQVDIEPLPELDSSDLQDWLDELDERISEDAWLGGWSLGGMLASELAAR
RGEHCCGLLTLASNPSFVAHEQWQSAMPGETFDAFLAGCSADPRTTLKRFSLLCAQGAQDARGLSRLLLGGAPNTSPAAL
MAGLELLAQIDTRAALQAFRGPQLHLFGGLDGLVPAEAAGELFALLADVEVGLIEQASHAFLLEDPHGVAGAIQAFLSEC
GDD

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family [H]

Homologues:

Organism=Escherichia coli, GI1789817, Length=237, Percent_Identity=32.9113924050633, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010076 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 25713; Mature: 25713

Theoretical pI: Translated: 4.06; Mature: 4.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDRLILLPGWGLGVSPLEPLAAALRGLDEHLQVDIEPLPELDSSDLQDWLDELDERISE
CCCCEEEECCCCCCCCCHHHHHHHHHCCHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHH
DAWLGGWSLGGMLASELAARRGEHCCGLLTLASNPSFVAHEQWQSAMPGETFDAFLAGCS
CCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEHHHHHHCCCCCHHHHHHHCCC
ADPRTTLKRFSLLCAQGAQDARGLSRLLLGGAPNTSPAALMAGLELLAQIDTRAALQAFR
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GPQLHLFGGLDGLVPAEAAGELFALLADVEVGLIEQASHAFLLEDPHGVAGAIQAFLSEC
CCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHH
GDD
CCC
>Mature Secondary Structure
MRDRLILLPGWGLGVSPLEPLAAALRGLDEHLQVDIEPLPELDSSDLQDWLDELDERISE
CCCCEEEECCCCCCCCCHHHHHHHHHCCHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHH
DAWLGGWSLGGMLASELAARRGEHCCGLLTLASNPSFVAHEQWQSAMPGETFDAFLAGCS
CCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEHHHHHHCCCCCHHHHHHHCCC
ADPRTTLKRFSLLCAQGAQDARGLSRLLLGGAPNTSPAALMAGLELLAQIDTRAALQAFR
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GPQLHLFGGLDGLVPAEAAGELFALLADVEVGLIEQASHAFLLEDPHGVAGAIQAFLSEC
CCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHH
GDD
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA