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The map label for this gene is mltB [H]

Identifier: 77461189

GI number: 77461189

Start: 5595930

End: 5596940

Strand: Reverse

Name: mltB [H]

Synonym: Pfl01_4968

Alternate gene names: 77461189

Gene position: 5596940-5595930 (Counterclockwise)

Preceding gene: 77461190

Following gene: 77461188

Centisome position: 86.93

GC content: 60.83

Gene sequence:

>1011_bases
ATGCAAGTAATGCGTGACTGGGCGACACGATACGCACCGTGGCTCGGCCTGGTAGGCATCCTTGGCAGCGTGCAGGAAGC
GTTGGCCGGCGATTACGAAGGCTCGCCCCAGGTGGCCGAATTCGTCGGTGAAATGACCCGCGACTACGGTTTCGCCGGCG
AACAGCTGATGGCCGTGTTCCGCGAGGCCCAGCGCAAACAGGCGATTCTCGACGCCATTTCCAAACCCGCCGAACGGGTC
AAGCAGTGGAAAGAATACCGGCCGATGTTCATCACCGACGCGCGCATCGCCCGCGGTGTGGATTTCTGGCGTCAGCACGA
GGCCACACTGGCCCGCGCCGAGCAGGAATACGGCGTTCCGGCCCAAGTGATCGTGTCGATCATCGGCGTTGAGACCTTTT
TCGGACGTAATACCGGTAATTACCGGGTGATCGATGCCTTGTCCACGCTGGGTTTCGACTATCCTCCTCGTGCCGAATTT
TTCCGCAAGGAGCTGCGTGAGTTCCTGCTGCTGGCGCGCGAGGAGCAGGTCGACCCGCTGACCCTCAAGGGTTCCTACGC
CGGGGCAATGGGTTTGCCACAGTTCATGCCGAGCAGTTTCCGCGCCTACGCGGTGGATTTCGACGGTGATGGCCACATCA
ACATCTGGAACAACCCGGATGATGCGATCGGCAGCGTTGCCAGCTATTTCAAACGTCACGGCTGGGTCGCCGGCGAGCCG
GTGGTCAGTCGCGCGGATGTACGCGGTGAGCAGGTGGATGAAGGCCTGACCACCGGCATCGAGCCGACGAAAACCGTCGG
GGAGTTGCGAGCGCTGGGCTGGTCGAGTCATGATGCGCTGCGCGATGACATGCCGGTCACCGCATTCCGCCTGGAAGGCG
ACAATGGCCCGGAATACTGGATGGGCCTGAAGAATTTCTACGCGATCACGCGTTATAACCGCAGCGTGATGTACGCCATG
GCCGTACATCAACTGTCTGAACAGCTGGTACAAGCACGGGGCGTCAAGTAA

Upstream 100 bases:

>100_bases
GAACTTCGCTGGTGACGCTGCTGTCAGCGTTTGGGGTTTTGATGTCGATCCATACCCATCGCAAGTGGATCGCACAGGTT
TGAATAAGGTGAAGAGTTCA

Downstream 100 bases:

>100_bases
TGCGGGCATTGCCTATCAATAAACCCCTGAAGCTGGTGGCTTTCGCTGCGTTGGCGGTGCTGGTCGCCAGTTGTTCGACC
AGCCGTTCGCCGAGCCAGAA

Product: lytic murein transglycosylase B

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MQVMRDWATRYAPWLGLVGILGSVQEALAGDYEGSPQVAEFVGEMTRDYGFAGEQLMAVFREAQRKQAILDAISKPAERV
KQWKEYRPMFITDARIARGVDFWRQHEATLARAEQEYGVPAQVIVSIIGVETFFGRNTGNYRVIDALSTLGFDYPPRAEF
FRKELREFLLLAREEQVDPLTLKGSYAGAMGLPQFMPSSFRAYAVDFDGDGHINIWNNPDDAIGSVASYFKRHGWVAGEP
VVSRADVRGEQVDEGLTTGIEPTKTVGELRALGWSSHDALRDDMPVTAFRLEGDNGPEYWMGLKNFYAITRYNRSVMYAM
AVHQLSEQLVQARGVK

Sequences:

>Translated_336_residues
MQVMRDWATRYAPWLGLVGILGSVQEALAGDYEGSPQVAEFVGEMTRDYGFAGEQLMAVFREAQRKQAILDAISKPAERV
KQWKEYRPMFITDARIARGVDFWRQHEATLARAEQEYGVPAQVIVSIIGVETFFGRNTGNYRVIDALSTLGFDYPPRAEF
FRKELREFLLLAREEQVDPLTLKGSYAGAMGLPQFMPSSFRAYAVDFDGDGHINIWNNPDDAIGSVASYFKRHGWVAGEP
VVSRADVRGEQVDEGLTTGIEPTKTVGELRALGWSSHDALRDDMPVTAFRLEGDNGPEYWMGLKNFYAITRYNRSVMYAM
AVHQLSEQLVQARGVK
>Mature_336_residues
MQVMRDWATRYAPWLGLVGILGSVQEALAGDYEGSPQVAEFVGEMTRDYGFAGEQLMAVFREAQRKQAILDAISKPAERV
KQWKEYRPMFITDARIARGVDFWRQHEATLARAEQEYGVPAQVIVSIIGVETFFGRNTGNYRVIDALSTLGFDYPPRAEF
FRKELREFLLLAREEQVDPLTLKGSYAGAMGLPQFMPSSFRAYAVDFDGDGHINIWNNPDDAIGSVASYFKRHGWVAGEP
VVSRADVRGEQVDEGLTTGIEPTKTVGELRALGWSSHDALRDDMPVTAFRLEGDNGPEYWMGLKNFYAITRYNRSVMYAM
AVHQLSEQLVQARGVK

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=315, Percent_Identity=46.6666666666667, Blast_Score=282, Evalue=2e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 37826; Mature: 37826

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVMRDWATRYAPWLGLVGILGSVQEALAGDYEGSPQVAEFVGEMTRDYGFAGEQLMAVF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHH
REAQRKQAILDAISKPAERVKQWKEYRPMFITDARIARGVDFWRQHEATLARAEQEYGVP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AQVIVSIIGVETFFGRNTGNYRVIDALSTLGFDYPPRAEFFRKELREFLLLAREEQVDPL
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCE
TLKGSYAGAMGLPQFMPSSFRAYAVDFDGDGHINIWNNPDDAIGSVASYFKRHGWVAGEP
EECCCCCCCCCCHHHCCCCCEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCC
VVSRADVRGEQVDEGLTTGIEPTKTVGELRALGWSSHDALRDDMPVTAFRLEGDNGPEYW
CHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHCCCCEEEEEEECCCCHHHH
MGLKNFYAITRYNRSVMYAMAVHQLSEQLVQARGVK
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQVMRDWATRYAPWLGLVGILGSVQEALAGDYEGSPQVAEFVGEMTRDYGFAGEQLMAVF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHH
REAQRKQAILDAISKPAERVKQWKEYRPMFITDARIARGVDFWRQHEATLARAEQEYGVP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AQVIVSIIGVETFFGRNTGNYRVIDALSTLGFDYPPRAEFFRKELREFLLLAREEQVDPL
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCE
TLKGSYAGAMGLPQFMPSSFRAYAVDFDGDGHINIWNNPDDAIGSVASYFKRHGWVAGEP
EECCCCCCCCCCHHHCCCCCEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCC
VVSRADVRGEQVDEGLTTGIEPTKTVGELRALGWSSHDALRDDMPVTAFRLEGDNGPEYW
CHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHCCCCEEEEEEECCCCHHHH
MGLKNFYAITRYNRSVMYAMAVHQLSEQLVQARGVK
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]