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The map label for this gene is fsr [H]

Identifier: 77460978

GI number: 77460978

Start: 5360667

End: 5361884

Strand: Reverse

Name: fsr [H]

Synonym: Pfl01_4757

Alternate gene names: 77460978

Gene position: 5361884-5360667 (Counterclockwise)

Preceding gene: 77460984

Following gene: 77460970

Centisome position: 83.28

GC content: 59.77

Gene sequence:

>1218_bases
ATGGCTATCAGCAATGTTCAGACCGCCGCTGCCTCGGCGTCCGCTCCTCCCCAGAGCAGCCCTTTGGTGATGCGCATCAT
TGGCGCGGTGGCGCTGGCGCATTTGATCAATGACCTGATCCAGTCAGTGTTGCCGTCGATCTATCCGATGCTCAAGGCCA
ACTATGGTTTGAGTTTTACCCAGGTCGGCCTGATTACCCTGACCTTCCAATTGACCGCTTCGCTGCTGCAGCCGTGGGTC
GGTTATCACACCGATCGCCATCCGAAACCGTGGCTGTTGCCGGCAGGTTCTGTGTGCACCCTGATTGGCATCGTGATGAT
GTCGATGGTTGGCAGTTTTCCGTTGATTCTGCTGGCGGCGGCGCTGATCGGCATTGGTTCATCAACCTTTCACCCCGAGG
CTTCTCGTATCGCCCGCCTGGCTTCTGGTGGGCGTTTCGGTCTGGCGCAGTCGACGTTCCAGGTCGGTGGTAATGCCGGT
TCGGCCTTCGGCCCGTTACTCGCGGCGGCGATCATCATTCCTTTCGGGCAGGGCAACGTGGCCTGGTTCGGCTTGTTCGC
GGTGTTTGCGCTGTTCGTGCTCTATCGCATCAGCCGCTGGTACGCCCATCACCTGAACCTGTTCAAGCTCAAGGCCGGCC
AGGCCGCCACTCACGGACTGTCGAAGGGCAGGGTGATCAGCGCTCTGGTGGTGCTCGGTCTGCTGGTGTTCTCCAAGTAC
TTCTACATGGCCAGCCTGACCAGCTACTTCACCTTCTACCTGATCGAGAAGTTCGACCTGTCGGTTGCCAGTTCCCAATT
GCACCTGTTCCTGTTCCTCGGCGCAGTGGCGGCGGGCACGTTCTTTGGCGGACCTATCGGCGACAAGATCGGGCGCAAGG
CGGTGATCTGGTTCTCGATCCTTGGCGTGGCACCGTTCACGCTGCTGATGCCGCATGTCGACCTGTTCTGGACCAGCGTT
CTGAGCGTGGTGATCGGTTTCATCCTCGCTTCAGCGTTCTCGGCGATCGTGGTGTACGCGCAGGAGCTGGTGCCGGGCAA
CGTCGGAATGATTGCCGGGGTGTTCTTCGGTCTGATGTTCGGCTTTGGCGGGATTGGCGCGGCGTTGCTCGGGCATCTGG
CGGACATCCACGGCATCGAATACGTGTACTTCCTGTGCTCGTTCCTGCCGCTGTTCGGGGTGCTGACGATCTTCCTGCCG
AGAACCAAAAAAGCCTGA

Upstream 100 bases:

>100_bases
AATAGTTCGTTAGCTGGCTAAGAGGATGGCGCTGACGCACCGAAAAATGGCACATTGACGCACCTGCCCGCGTGCACCCA
TCTGCAGTCCGGAAATTCCC

Downstream 100 bases:

>100_bases
CAGGCTGAATTTCGGGCAAAAAAAAGCCGCGTATCAACGCGGCTTTTTCTTGGGTGCAGCTTTTACACGTTGAAGCGGAA
GTGCATCACGTCGCCGTCCT

Product: major facilitator transporter

Products: fosmidomycin [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MAISNVQTAAASASAPPQSSPLVMRIIGAVALAHLINDLIQSVLPSIYPMLKANYGLSFTQVGLITLTFQLTASLLQPWV
GYHTDRHPKPWLLPAGSVCTLIGIVMMSMVGSFPLILLAAALIGIGSSTFHPEASRIARLASGGRFGLAQSTFQVGGNAG
SAFGPLLAAAIIIPFGQGNVAWFGLFAVFALFVLYRISRWYAHHLNLFKLKAGQAATHGLSKGRVISALVVLGLLVFSKY
FYMASLTSYFTFYLIEKFDLSVASSQLHLFLFLGAVAAGTFFGGPIGDKIGRKAVIWFSILGVAPFTLLMPHVDLFWTSV
LSVVIGFILASAFSAIVVYAQELVPGNVGMIAGVFFGLMFGFGGIGAALLGHLADIHGIEYVYFLCSFLPLFGVLTIFLP
RTKKA

Sequences:

>Translated_405_residues
MAISNVQTAAASASAPPQSSPLVMRIIGAVALAHLINDLIQSVLPSIYPMLKANYGLSFTQVGLITLTFQLTASLLQPWV
GYHTDRHPKPWLLPAGSVCTLIGIVMMSMVGSFPLILLAAALIGIGSSTFHPEASRIARLASGGRFGLAQSTFQVGGNAG
SAFGPLLAAAIIIPFGQGNVAWFGLFAVFALFVLYRISRWYAHHLNLFKLKAGQAATHGLSKGRVISALVVLGLLVFSKY
FYMASLTSYFTFYLIEKFDLSVASSQLHLFLFLGAVAAGTFFGGPIGDKIGRKAVIWFSILGVAPFTLLMPHVDLFWTSV
LSVVIGFILASAFSAIVVYAQELVPGNVGMIAGVFFGLMFGFGGIGAALLGHLADIHGIEYVYFLCSFLPLFGVLTIFLP
RTKKA
>Mature_404_residues
AISNVQTAAASASAPPQSSPLVMRIIGAVALAHLINDLIQSVLPSIYPMLKANYGLSFTQVGLITLTFQLTASLLQPWVG
YHTDRHPKPWLLPAGSVCTLIGIVMMSMVGSFPLILLAAALIGIGSSTFHPEASRIARLASGGRFGLAQSTFQVGGNAGS
AFGPLLAAAIIIPFGQGNVAWFGLFAVFALFVLYRISRWYAHHLNLFKLKAGQAATHGLSKGRVISALVVLGLLVFSKYF
YMASLTSYFTFYLIEKFDLSVASSQLHLFLFLGAVAAGTFFGGPIGDKIGRKAVIWFSILGVAPFTLLMPHVDLFWTSVL
SVVIGFILASAFSAIVVYAQELVPGNVGMIAGVFFGLMFGFGGIGAALLGHLADIHGIEYVYFLCSFLPLFGVLTIFLPR
TKKA

Specific function: Confers the resistance against fosmidomycin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1786686, Length=403, Percent_Identity=59.3052109181141, Blast_Score=452, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43163; Mature: 43032

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAISNVQTAAASASAPPQSSPLVMRIIGAVALAHLINDLIQSVLPSIYPMLKANYGLSFT
CCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
QVGLITLTFQLTASLLQPWVGYHTDRHPKPWLLPAGSVCTLIGIVMMSMVGSFPLILLAA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
ALIGIGSSTFHPEASRIARLASGGRFGLAQSTFQVGGNAGSAFGPLLAAAIIIPFGQGNV
HHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCH
AWFGLFAVFALFVLYRISRWYAHHLNLFKLKAGQAATHGLSKGRVISALVVLGLLVFSKY
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHCCCCCCHHHHHHHHHHHHHHHHH
FYMASLTSYFTFYLIEKFDLSVASSQLHLFLFLGAVAAGTFFGGPIGDKIGRKAVIWFSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHH
LGVAPFTLLMPHVDLFWTSVLSVVIGFILASAFSAIVVYAQELVPGNVGMIAGVFFGLMF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
GFGGIGAALLGHLADIHGIEYVYFLCSFLPLFGVLTIFLPRTKKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AISNVQTAAASASAPPQSSPLVMRIIGAVALAHLINDLIQSVLPSIYPMLKANYGLSFT
CCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
QVGLITLTFQLTASLLQPWVGYHTDRHPKPWLLPAGSVCTLIGIVMMSMVGSFPLILLAA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
ALIGIGSSTFHPEASRIARLASGGRFGLAQSTFQVGGNAGSAFGPLLAAAIIIPFGQGNV
HHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCH
AWFGLFAVFALFVLYRISRWYAHHLNLFKLKAGQAATHGLSKGRVISALVVLGLLVFSKY
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHCCCCCCHHHHHHHHHHHHHHHHH
FYMASLTSYFTFYLIEKFDLSVASSQLHLFLFLGAVAAGTFFGGPIGDKIGRKAVIWFSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHH
LGVAPFTLLMPHVDLFWTSVLSVVIGFILASAFSAIVVYAQELVPGNVGMIAGVFFGLMF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
GFGGIGAALLGHLADIHGIEYVYFLCSFLPLFGVLTIFLPRTKKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: fosmidomycin [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: fosmidomycin [Cytoplasm] + Proton [Periplasm] = fosmidomycin [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8917080; 9278503 [H]