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The map label for this gene is murE [H]

Identifier: 77460899

GI number: 77460899

Start: 5281226

End: 5282689

Strand: Reverse

Name: murE [H]

Synonym: Pfl01_4678

Alternate gene names: 77460899

Gene position: 5282689-5281226 (Counterclockwise)

Preceding gene: 77460900

Following gene: 77460898

Centisome position: 82.05

GC content: 63.66

Gene sequence:

>1464_bases
ATGTCTCTGAGTCTGAACAAGATTTTCCCCCACGCCGGCCACGATCTGTTGATCCGTGAACTGGCGCTCGACAGCCGCAA
CGTGCGCGCGGGTGACCTGTTCCTCGCCGTGCCGGGCGGCAGGTTCGACGGTCGTGCGCACATTGCCGACGCCTTGCAGC
GCGGTGCTGCTGCCGTGGCTTATGAAGTGGAAGGCGCCACCGTGCTGCCGATCACCGAAGTGCCGCTGATTCCGGTCAAG
GGCCTGGCGGCGCAGTTGTCGGACATCGCCGGGCGTTTTTACGGTGAGCCGAGTCATCATCTGAATCTGATCGGCGTCAC
TGGCACCAACGGCAAGACCAGCGTGACTCAACTGGTGGCGCAGGCGCTGGATCTGCTCGGTCAGCATTGCGGCATCGTCG
GCACCTTGGGCTCCGGTTTCCATGGCTCGCTGCAAAGCGGCCTGCACACGACGCCGAATCCGATCGCCATGCAAGCGACC
CTGGGCGACCTGAAAAAGGCCGGCGCCAAAGCCGTGGCCATGGAAGTTTCGTCCCACGGCCTGGATCAGGGCCGGGTTAC
TGCGCTGGCGTTCGACGTGGCAGTGATGACCAACCTGTCCCGCGATCACCTGGATTATCACGGCACCATGGAGGCCTACG
CGGAAGCGAAGGCCAAGCTGTTTGCCTGGAATGACCTGAAGTGCCGCGTGGTCAACCTCGACGACGACTTCGGCCGGCAA
CTGGCCGCTGAAAAGCGCGAGTCGCGGCTGATCACTTACAGCCTGCTCGACAGCAGCGCCTATCTCTATGTGCGTGAAGC
GCAGTTCGACGACCATGGCGTGCGCGCCACGCTGGTCACGCCACAGGGCGAGCACCATCTGCGCAGCACGCTGCTCGGTC
GTTTCAACCTGAGCAACGTACTCGCCGCCGTCGGCGCCTTGCTCGGTCTCGACTACGCGCTCGACGAAATCCTTAAGGTA
TTGCCGAAACTCGAAGGCCCGGCCGGACGCATGCAGCGTCTCGGCGGCGGCACTCAGCCGCTGGTGGTGGTCGATTACGC
GCATACCCCGGACGCCCTGGAAAAAGTACTGACCGCGTTGCGTCCGCACGTCAAAGGTCAACTGCTGTGCCTGTTCGGCT
GCGGCGGTGATCGCGATCGCGGCAAGCGTCCGCTGATGGCCGAAGTGGTCGAGCGTCTGGCCGACCGCGTGCTGGTGACC
GATGACAATCCGCGCACCGAAGACCCGGCGGTAATTTTCGATGACATCCGCGCCGGCTTCACGGCTGTGGATAACGTGAC
GTTCGTGGCTGGCCGTGGCCAGGCCATCGCGCAACTGATCGCCGGCGCTTCGGCCGACGACGTGATTGTCCTGGCCGGCA
AAGGTCACGAGGACTATCAGGAAATCAACGGCGAGCGCCATGCATTCTCGGATCTGGTCGAGGCGGATCACGCCCTGACC
GCGTGGGAGGTGGCCCATGCTTAA

Upstream 100 bases:

>100_bases
CCCTGCGCCTGATGAACGTCACCCCGGACAACCTGCCGACCACACAACAGGCCAACGCCACCCCGGCCGTTCCGCTGAAA
GCCAATGGAGGGCGCGGCTG

Downstream 100 bases:

>100_bases
GGCCCTGAAACTCAGCGAACTGACCAACGCGCTCGACGCCCGTCTGATCAGCGCCGATGCCAGTTTTGACGGTGTGAGCA
TCGACAGCCGCGCGATCAAG

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MSLSLNKIFPHAGHDLLIRELALDSRNVRAGDLFLAVPGGRFDGRAHIADALQRGAAAVAYEVEGATVLPITEVPLIPVK
GLAAQLSDIAGRFYGEPSHHLNLIGVTGTNGKTSVTQLVAQALDLLGQHCGIVGTLGSGFHGSLQSGLHTTPNPIAMQAT
LGDLKKAGAKAVAMEVSSHGLDQGRVTALAFDVAVMTNLSRDHLDYHGTMEAYAEAKAKLFAWNDLKCRVVNLDDDFGRQ
LAAEKRESRLITYSLLDSSAYLYVREAQFDDHGVRATLVTPQGEHHLRSTLLGRFNLSNVLAAVGALLGLDYALDEILKV
LPKLEGPAGRMQRLGGGTQPLVVVDYAHTPDALEKVLTALRPHVKGQLLCLFGCGGDRDRGKRPLMAEVVERLADRVLVT
DDNPRTEDPAVIFDDIRAGFTAVDNVTFVAGRGQAIAQLIAGASADDVIVLAGKGHEDYQEINGERHAFSDLVEADHALT
AWEVAHA

Sequences:

>Translated_487_residues
MSLSLNKIFPHAGHDLLIRELALDSRNVRAGDLFLAVPGGRFDGRAHIADALQRGAAAVAYEVEGATVLPITEVPLIPVK
GLAAQLSDIAGRFYGEPSHHLNLIGVTGTNGKTSVTQLVAQALDLLGQHCGIVGTLGSGFHGSLQSGLHTTPNPIAMQAT
LGDLKKAGAKAVAMEVSSHGLDQGRVTALAFDVAVMTNLSRDHLDYHGTMEAYAEAKAKLFAWNDLKCRVVNLDDDFGRQ
LAAEKRESRLITYSLLDSSAYLYVREAQFDDHGVRATLVTPQGEHHLRSTLLGRFNLSNVLAAVGALLGLDYALDEILKV
LPKLEGPAGRMQRLGGGTQPLVVVDYAHTPDALEKVLTALRPHVKGQLLCLFGCGGDRDRGKRPLMAEVVERLADRVLVT
DDNPRTEDPAVIFDDIRAGFTAVDNVTFVAGRGQAIAQLIAGASADDVIVLAGKGHEDYQEINGERHAFSDLVEADHALT
AWEVAHA
>Mature_486_residues
SLSLNKIFPHAGHDLLIRELALDSRNVRAGDLFLAVPGGRFDGRAHIADALQRGAAAVAYEVEGATVLPITEVPLIPVKG
LAAQLSDIAGRFYGEPSHHLNLIGVTGTNGKTSVTQLVAQALDLLGQHCGIVGTLGSGFHGSLQSGLHTTPNPIAMQATL
GDLKKAGAKAVAMEVSSHGLDQGRVTALAFDVAVMTNLSRDHLDYHGTMEAYAEAKAKLFAWNDLKCRVVNLDDDFGRQL
AAEKRESRLITYSLLDSSAYLYVREAQFDDHGVRATLVTPQGEHHLRSTLLGRFNLSNVLAAVGALLGLDYALDEILKVL
PKLEGPAGRMQRLGGGTQPLVVVDYAHTPDALEKVLTALRPHVKGQLLCLFGCGGDRDRGKRPLMAEVVERLADRVLVTD
DNPRTEDPAVIFDDIRAGFTAVDNVTFVAGRGQAIAQLIAGASADDVIVLAGKGHEDYQEINGERHAFSDLVEADHALTA
WEVAHA

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=466, Percent_Identity=45.4935622317597, Blast_Score=340, Evalue=1e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 52070; Mature: 51939

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSLNKIFPHAGHDLLIRELALDSRNVRAGDLFLAVPGGRFDGRAHIADALQRGAAAVA
CCCCCCCCCCCCCHHHHHHHHHCCCCCCEECEEEEECCCCCCCCHHHHHHHHHCCCEEEE
YEVEGATVLPITEVPLIPVKGLAAQLSDIAGRFYGEPSHHLNLIGVTGTNGKTSVTQLVA
EEECCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHH
QALDLLGQHCGIVGTLGSGFHGSLQSGLHTTPNPIAMQATLGDLKKAGAKAVAMEVSSHG
HHHHHHHHHCCEEECCCCCCCCHHHCCCCCCCCCEEEEHHHHHHHHCCCCEEEEEHHHCC
LDQGRVTALAFDVAVMTNLSRDHLDYHGTMEAYAEAKAKLFAWNDLKCRVVNLDDDFGRQ
CCCCCEEEEEEHHHHHHCCCHHHCCCCCHHHHHHHHHHEEEEECCCEEEEEECCHHHHHH
LAAEKRESRLITYSLLDSSAYLYVREAQFDDHGVRATLVTPQGEHHLRSTLLGRFNLSNV
HHHHHHHCEEEEEEEECCCEEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHCCCHHHH
LAAVGALLGLDYALDEILKVLPKLEGPAGRMQRLGGGTQPLVVVDYAHTPDALEKVLTAL
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHH
RPHVKGQLLCLFGCGGDRDRGKRPLMAEVVERLADRVLVTDDNPRTEDPAVIFDDIRAGF
CCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCEEEECCCCCCCCCCEEEHHHHCCC
TAVDNVTFVAGRGQAIAQLIAGASADDVIVLAGKGHEDYQEINGERHAFSDLVEADHALT
EECCCEEEEECCCHHHHHHHHCCCCCCEEEEECCCCHHHHHHCCCHHHHHHHHHHCCCHH
AWEVAHA
HEEECCC
>Mature Secondary Structure 
SLSLNKIFPHAGHDLLIRELALDSRNVRAGDLFLAVPGGRFDGRAHIADALQRGAAAVA
CCCCCCCCCCCCHHHHHHHHHCCCCCCEECEEEEECCCCCCCCHHHHHHHHHCCCEEEE
YEVEGATVLPITEVPLIPVKGLAAQLSDIAGRFYGEPSHHLNLIGVTGTNGKTSVTQLVA
EEECCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHH
QALDLLGQHCGIVGTLGSGFHGSLQSGLHTTPNPIAMQATLGDLKKAGAKAVAMEVSSHG
HHHHHHHHHCCEEECCCCCCCCHHHCCCCCCCCCEEEEHHHHHHHHCCCCEEEEEHHHCC
LDQGRVTALAFDVAVMTNLSRDHLDYHGTMEAYAEAKAKLFAWNDLKCRVVNLDDDFGRQ
CCCCCEEEEEEHHHHHHCCCHHHCCCCCHHHHHHHHHHEEEEECCCEEEEEECCHHHHHH
LAAEKRESRLITYSLLDSSAYLYVREAQFDDHGVRATLVTPQGEHHLRSTLLGRFNLSNV
HHHHHHHCEEEEEEEECCCEEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHCCCHHHH
LAAVGALLGLDYALDEILKVLPKLEGPAGRMQRLGGGTQPLVVVDYAHTPDALEKVLTAL
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHH
RPHVKGQLLCLFGCGGDRDRGKRPLMAEVVERLADRVLVTDDNPRTEDPAVIFDDIRAGF
CCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCEEEECCCCCCCCCCEEEHHHHCCC
TAVDNVTFVAGRGQAIAQLIAGASADDVIVLAGKGHEDYQEINGERHAFSDLVEADHALT
EECCCEEEEECCCHHHHHHHHCCCCCCEEEEECCCCHHHHHHCCCHHHHHHHHHHCCCHH
AWEVAHA
HEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]