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The map label for this gene is murF [H]

Identifier: 77460898

GI number: 77460898

Start: 5279866

End: 5281233

Strand: Reverse

Name: murF [H]

Synonym: Pfl01_4677

Alternate gene names: 77460898

Gene position: 5281233-5279866 (Counterclockwise)

Preceding gene: 77460899

Following gene: 77460897

Centisome position: 82.03

GC content: 62.43

Gene sequence:

>1368_bases
ATGCTTAAGGCCCTGAAACTCAGCGAACTGACCAACGCGCTCGACGCCCGTCTGATCAGCGCCGATGCCAGTTTTGACGG
TGTGAGCATCGACAGCCGCGCGATCAAGGCTGGCCAACTGTTTATCGCCCTGACCGGTCCGCGTTTCGACGGTCATGACT
ATTTGAATGAAGTCGCCGCCAAAGGCGCCGTTGCGGCATTGGTCGAACGTGAAGTCGCCGACAGCACGCTGCCGCAATTG
CTGGTCAAGGATACCCGTCAGGCTCTCGGCCAGTTGGGTGCCTTGAACCGTGCCGCATTCACCAAACCGGTTGCGGCAGT
CACCGGCTCCAGCGGCAAGACCACGGTCAAGGAAATGCTCGCGAGCATCCTGCGCACACGCGGTTCGGTACACGCGACCC
GTGGCAACCTGAACAACGACCTCGGCGTACCGCTGACCCTGCTCGAACTCGCCCCGGAACACAGCGCGGCAGTGATCGAA
CTGGGCGCCTCGCGTCTAGGTGAAATCGCCTACACCGTAGGGCTGACCAAGCCGCACGTAGCGATCCTCAACAACGCCGG
AACCGCCCACGTCGGTGAGTTCGGCGGGCCGGAGAAAATCGTCGAAGCCAAAGGCGAAATCATCGAAGGGCTGGCGGCCG
ACGGCGTCGCTGTGCTGAACCTCGATGACAAGGCGTTTGGCATCTGGAAGACCCGCGCTGCCGGTCGCAAAGTGCTGACT
TTCGCATTGAGCAACACTCAGGCGGACTTCCATGCCAGCGATCTGGCCACCGATGCCCGTGGCTGCCCGGCGTTCAACCT
GCACAGTCCTGAAGGTGTGGAACGGGTTCAACTGAACCTGCTCGGCACTCATAACGTCGCCAACGCCATGGCCGCTGCTG
CTGCTGCGCACGCCTTGGGTGTGTCGCTGTTCGGCATCGCCACCGGGCTTGGCGCGGTACAACCGGTCAAGGGTCGCACC
GTCGCGCAACTGGCGAAAAACGGCATGCGCGTGATCGATGACACTTACAACGCGAACCCCACCTCGATGTGCGCGGCCGT
TGATATACTCGCCGGCTTTTCCGGCCGCACCGTCCTGGTGCTCGGGGATATCGGCGAGTTGGGCGACTGGGCGGAGCAGG
GGCATCGCGACGTGGGCGAATACGCCCGGGGCAAGGTTTCCGCGCTATACGCCGTCGGGCCGAACATGGTCCACGCCGTG
AGCGCATTCGGTAACGAGGCGCAGCACTTCGGCACACAGGCCGAACTGATCCAGGCTCTCGGCGCCGAGCAGGACACAAA
CACCACCATTTTGATCAAGGGTTCGCGCAGCGCAGCGATGGAAAACATCGTTGCGGCCCTGTGCGGGTCCAGTCTGGAGA
AACATTAA

Upstream 100 bases:

>100_bases
GGCAAAGGTCACGAGGACTATCAGGAAATCAACGGCGAGCGCCATGCATTCTCGGATCTGGTCGAGGCGGATCACGCCCT
GACCGCGTGGGAGGTGGCCC

Downstream 100 bases:

>100_bases
TGCTGCTGCTGCTAGCGGAGTATCTGCAACAGTTCTACAAAGGCTTCGCGGTCTTCCAGTACCTGACCCTGCGCGGGATC
CTCGGTGTGCTGACCGCGCT

Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MLKALKLSELTNALDARLISADASFDGVSIDSRAIKAGQLFIALTGPRFDGHDYLNEVAAKGAVAALVEREVADSTLPQL
LVKDTRQALGQLGALNRAAFTKPVAAVTGSSGKTTVKEMLASILRTRGSVHATRGNLNNDLGVPLTLLELAPEHSAAVIE
LGASRLGEIAYTVGLTKPHVAILNNAGTAHVGEFGGPEKIVEAKGEIIEGLAADGVAVLNLDDKAFGIWKTRAAGRKVLT
FALSNTQADFHASDLATDARGCPAFNLHSPEGVERVQLNLLGTHNVANAMAAAAAAHALGVSLFGIATGLGAVQPVKGRT
VAQLAKNGMRVIDDTYNANPTSMCAAVDILAGFSGRTVLVLGDIGELGDWAEQGHRDVGEYARGKVSALYAVGPNMVHAV
SAFGNEAQHFGTQAELIQALGAEQDTNTTILIKGSRSAAMENIVAALCGSSLEKH

Sequences:

>Translated_455_residues
MLKALKLSELTNALDARLISADASFDGVSIDSRAIKAGQLFIALTGPRFDGHDYLNEVAAKGAVAALVEREVADSTLPQL
LVKDTRQALGQLGALNRAAFTKPVAAVTGSSGKTTVKEMLASILRTRGSVHATRGNLNNDLGVPLTLLELAPEHSAAVIE
LGASRLGEIAYTVGLTKPHVAILNNAGTAHVGEFGGPEKIVEAKGEIIEGLAADGVAVLNLDDKAFGIWKTRAAGRKVLT
FALSNTQADFHASDLATDARGCPAFNLHSPEGVERVQLNLLGTHNVANAMAAAAAAHALGVSLFGIATGLGAVQPVKGRT
VAQLAKNGMRVIDDTYNANPTSMCAAVDILAGFSGRTVLVLGDIGELGDWAEQGHRDVGEYARGKVSALYAVGPNMVHAV
SAFGNEAQHFGTQAELIQALGAEQDTNTTILIKGSRSAAMENIVAALCGSSLEKH
>Mature_455_residues
MLKALKLSELTNALDARLISADASFDGVSIDSRAIKAGQLFIALTGPRFDGHDYLNEVAAKGAVAALVEREVADSTLPQL
LVKDTRQALGQLGALNRAAFTKPVAAVTGSSGKTTVKEMLASILRTRGSVHATRGNLNNDLGVPLTLLELAPEHSAAVIE
LGASRLGEIAYTVGLTKPHVAILNNAGTAHVGEFGGPEKIVEAKGEIIEGLAADGVAVLNLDDKAFGIWKTRAAGRKVLT
FALSNTQADFHASDLATDARGCPAFNLHSPEGVERVQLNLLGTHNVANAMAAAAAAHALGVSLFGIATGLGAVQPVKGRT
VAQLAKNGMRVIDDTYNANPTSMCAAVDILAGFSGRTVLVLGDIGELGDWAEQGHRDVGEYARGKVSALYAVGPNMVHAV
SAFGNEAQHFGTQAELIQALGAEQDTNTTILIKGSRSAAMENIVAALCGSSLEKH

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=449, Percent_Identity=45.8797327394209, Blast_Score=320, Evalue=1e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 47126; Mature: 47126

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKALKLSELTNALDARLISADASFDGVSIDSRAIKAGQLFIALTGPRFDGHDYLNEVAA
CCCCHHHHHHHHHHHHHEEECCCCCCCCCCCHHHEEECEEEEEEECCCCCCHHHHHHHHH
KGAVAALVEREVADSTLPQLLVKDTRQALGQLGALNRAAFTKPVAAVTGSSGKTTVKEML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHEEECCCCCHHHHHHH
ASILRTRGSVHATRGNLNNDLGVPLTLLELAPEHSAAVIELGASRLGEIAYTVGLTKPHV
HHHHHCCCCEEEECCCCCCCCCCCEEEEHHCCCCCHHHHHHHHHHHHHEEEEECCCCCCE
AILNNAGTAHVGEFGGPEKIVEAKGEIIEGLAADGVAVLNLDDKAFGIWKTRAAGRKVLT
EEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEECCCCCCEEEE
FALSNTQADFHASDLATDARGCPAFNLHSPEGVERVQLNLLGTHNVANAMAAAAAAHALG
EEECCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHH
VSLFGIATGLGAVQPVKGRTVAQLAKNGMRVIDDTYNANPTSMCAAVDILAGFSGRTVLV
HHHHHHHHCCCCCCCCCCCHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEEE
LGDIGELGDWAEQGHRDVGEYARGKVSALYAVGPNMVHAVSAFGNEAQHFGTQAELIQAL
EECCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHCCHHHHCCCHHHHHHHH
GAEQDTNTTILIKGSRSAAMENIVAALCGSSLEKH
CCCCCCCCEEEEECCHHHHHHHHHHHHHCCHHCCC
>Mature Secondary Structure
MLKALKLSELTNALDARLISADASFDGVSIDSRAIKAGQLFIALTGPRFDGHDYLNEVAA
CCCCHHHHHHHHHHHHHEEECCCCCCCCCCCHHHEEECEEEEEEECCCCCCHHHHHHHHH
KGAVAALVEREVADSTLPQLLVKDTRQALGQLGALNRAAFTKPVAAVTGSSGKTTVKEML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHEEECCCCCHHHHHHH
ASILRTRGSVHATRGNLNNDLGVPLTLLELAPEHSAAVIELGASRLGEIAYTVGLTKPHV
HHHHHCCCCEEEECCCCCCCCCCCEEEEHHCCCCCHHHHHHHHHHHHHEEEEECCCCCCE
AILNNAGTAHVGEFGGPEKIVEAKGEIIEGLAADGVAVLNLDDKAFGIWKTRAAGRKVLT
EEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEECCCCCCEEEE
FALSNTQADFHASDLATDARGCPAFNLHSPEGVERVQLNLLGTHNVANAMAAAAAAHALG
EEECCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHH
VSLFGIATGLGAVQPVKGRTVAQLAKNGMRVIDDTYNANPTSMCAAVDILAGFSGRTVLV
HHHHHHHHCCCCCCCCCCCHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEEE
LGDIGELGDWAEQGHRDVGEYARGKVSALYAVGPNMVHAVSAFGNEAQHFGTQAELIQAL
EECCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHCCHHHHCCCHHHHHHHH
GAEQDTNTTILIKGSRSAAMENIVAALCGSSLEKH
CCCCCCCCEEEEECCHHHHHHHHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2668880; 8973200; 1630901; 9278503; 9166795; 2186811; 11090285 [H]