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The map label for this gene is cusS [H]

Identifier: 77460885

GI number: 77460885

Start: 5263403

End: 5264767

Strand: Direct

Name: cusS [H]

Synonym: Pfl01_4664

Alternate gene names: 77460885

Gene position: 5263403-5264767 (Clockwise)

Preceding gene: 77460884

Following gene: 77460886

Centisome position: 81.75

GC content: 62.27

Gene sequence:

>1365_bases
GTGTCCAGTAATTCCATTGCGCTGCGCTTGAGCGGGATGTTCACGCTGGTGGCGCTGCTGGTGTTCCTGTTGATCGGCGG
CGCGCTGTATCAACAAGTGGACAAAGGCCTCGGCCTGCTGCCGGAAGCGGAGCTCGATGCGCGTTACAGCGTGCTGGAAT
CCGCGCTCAACCGCTATGGCACCCCTGAGCACTGGGTGAAGATCAACGCCAAGCTCAAGCTGCTCGGCGAAGAGGACAAG
CGCATCCGCTTCTGGGTGGTCAGCGGCAATCCGGGCTACGAGTTTGGCGAGCCGGATGCCTTGATCCGCGCGTTTGCCCA
AGGCCCCTTGGGCATGCGCGACCTGCAACTGCCCGACCATCCTTATCCGCTGAAAGTGCTGCTCACCGAATTGCCGGCCA
AGGATCAACGCCCGCCGTTGCGCTTCATGATCGGCATCGACACCGAGACCTTTCACCAGACCCAGCATCAGTTGCTGATC
GCGCTGGTGAGTCTGGCGGTGATCGGTGTGCTACTGGCGTCGGCATTGGGTTACTGGGTGGCGCGGATCGGCCTCAAACC
GTTGATCAAACTGTCCCACGAAGCCCAGCGTCTGGCCCCGCCGTTGCGGGCCGGGCGCCTGCGTCTGTCACCGTTGCCAC
CGGAACTGGAGCAGTTCGTCGAGTCGTTCAACTCGACGCTGGAGCGGGTCGAACAGGCGTACTCGCGGCTGGAGTCATTC
AACGCCGACGTCGCCCATGAACTGCGCTCGCCACTGACCAACCTGATCGGCCAGACCCAGGTCGCCCTGACGCGCGGGCG
CTCCGCCGAGCATTATTTCGAAGTGCTGCAATCCAACCTCGAAGAGCTGGAACGCCTGCGCTCGATCATCAACGACATGC
TGTTCCTGGCCAGCGCCGATCAGGGCAGCAAGGCCACCAAACTGACCTCGACCTCGCTGGCCGATGAAGTGGCAACCACC
CTCGAATACCTCGACTTCATTCTCGAAGATGCGCAGGTCCAGGTGCGGGTAAGCGGCGACGCGCGGGTACAGATCGAGAT
CGCCCATCTGCGCCGGGCCTTGATCAATCTGCTGAGCAACGCGGTGCAACACACGGCGCCCGGTCAGGTGATCGAAGTAA
AGATCGCGGCCGAGGAACATCAGGTCAGCATCGGCGTGGCCAACCCCGGTTTGCCGATTGCCAGCGAGCATTTGCCGCGC
TTGTTCGAGCGTTTCTACCGGGTCGATGCGTCGCGCAGCAACAGCGGCAACAATCACGGGTTGGGGCTGGCGATCGTCAA
GGCGATTGCGCTGATGCACGGCGGCGATGTGTTTGTGCGCAGTGATCGCGGGATGAACACCTTCGGGATCCACCTTCCGG
TCTGA

Upstream 100 bases:

>100_bases
GAAGTCGCGATCAAGCGCCTGCGAGCCAAGCTCGACGGGCCGTTCGACGAGAAACTGCTGCACACCATCCGTGGCATGGG
TTATGTGCTGGAGAGCCGTG

Downstream 100 bases:

>100_bases
GGTCAGGGATCATTGCCATTCCCGCAACAGTCATTTATGAAAATCACGCTGTTTCCCAACGCCCGGCAACCTTATCTTTG
CCCGCACCAAACAGCACTTG

Product: heavy metal sensor Signal transduction histidine kinase (STHK)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MSSNSIALRLSGMFTLVALLVFLLIGGALYQQVDKGLGLLPEAELDARYSVLESALNRYGTPEHWVKINAKLKLLGEEDK
RIRFWVVSGNPGYEFGEPDALIRAFAQGPLGMRDLQLPDHPYPLKVLLTELPAKDQRPPLRFMIGIDTETFHQTQHQLLI
ALVSLAVIGVLLASALGYWVARIGLKPLIKLSHEAQRLAPPLRAGRLRLSPLPPELEQFVESFNSTLERVEQAYSRLESF
NADVAHELRSPLTNLIGQTQVALTRGRSAEHYFEVLQSNLEELERLRSIINDMLFLASADQGSKATKLTSTSLADEVATT
LEYLDFILEDAQVQVRVSGDARVQIEIAHLRRALINLLSNAVQHTAPGQVIEVKIAAEEHQVSIGVANPGLPIASEHLPR
LFERFYRVDASRSNSGNNHGLGLAIVKAIALMHGGDVFVRSDRGMNTFGIHLPV

Sequences:

>Translated_454_residues
MSSNSIALRLSGMFTLVALLVFLLIGGALYQQVDKGLGLLPEAELDARYSVLESALNRYGTPEHWVKINAKLKLLGEEDK
RIRFWVVSGNPGYEFGEPDALIRAFAQGPLGMRDLQLPDHPYPLKVLLTELPAKDQRPPLRFMIGIDTETFHQTQHQLLI
ALVSLAVIGVLLASALGYWVARIGLKPLIKLSHEAQRLAPPLRAGRLRLSPLPPELEQFVESFNSTLERVEQAYSRLESF
NADVAHELRSPLTNLIGQTQVALTRGRSAEHYFEVLQSNLEELERLRSIINDMLFLASADQGSKATKLTSTSLADEVATT
LEYLDFILEDAQVQVRVSGDARVQIEIAHLRRALINLLSNAVQHTAPGQVIEVKIAAEEHQVSIGVANPGLPIASEHLPR
LFERFYRVDASRSNSGNNHGLGLAIVKAIALMHGGDVFVRSDRGMNTFGIHLPV
>Mature_453_residues
SSNSIALRLSGMFTLVALLVFLLIGGALYQQVDKGLGLLPEAELDARYSVLESALNRYGTPEHWVKINAKLKLLGEEDKR
IRFWVVSGNPGYEFGEPDALIRAFAQGPLGMRDLQLPDHPYPLKVLLTELPAKDQRPPLRFMIGIDTETFHQTQHQLLIA
LVSLAVIGVLLASALGYWVARIGLKPLIKLSHEAQRLAPPLRAGRLRLSPLPPELEQFVESFNSTLERVEQAYSRLESFN
ADVAHELRSPLTNLIGQTQVALTRGRSAEHYFEVLQSNLEELERLRSIINDMLFLASADQGSKATKLTSTSLADEVATTL
EYLDFILEDAQVQVRVSGDARVQIEIAHLRRALINLLSNAVQHTAPGQVIEVKIAAEEHQVSIGVANPGLPIASEHLPRL
FERFYRVDASRSNSGNNHGLGLAIVKAIALMHGGDVFVRSDRGMNTFGIHLPV

Specific function: Member of the two-component regulatory system CusS/CusR. Copper ion sensor. Could also be a silver ion sensor. Probably activates CusR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=323, Percent_Identity=40.8668730650155, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI1788279, Length=317, Percent_Identity=31.8611987381703, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI1786600, Length=217, Percent_Identity=31.3364055299539, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789403, Length=453, Percent_Identity=25.1655629139073, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788393, Length=258, Percent_Identity=27.5193798449612, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1790346, Length=260, Percent_Identity=30.7692307692308, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1787894, Length=303, Percent_Identity=26.7326732673267, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1790861, Length=299, Percent_Identity=25.0836120401338, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI145693157, Length=246, Percent_Identity=26.0162601626016, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790436, Length=221, Percent_Identity=28.5067873303167, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789149, Length=271, Percent_Identity=27.6752767527675, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI48994928, Length=249, Percent_Identity=27.710843373494, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786912, Length=262, Percent_Identity=28.2442748091603, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1790551, Length=295, Percent_Identity=26.7796610169492, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1789808, Length=297, Percent_Identity=25.5892255892256, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50177; Mature: 50045

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNSIALRLSGMFTLVALLVFLLIGGALYQQVDKGLGLLPEAELDARYSVLESALNRYG
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCC
TPEHWVKINAKLKLLGEEDKRIRFWVVSGNPGYEFGEPDALIRAFAQGPLGMRDLQLPDH
CCCCEEEEEEEEEEECCCCCEEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
PYPLKVLLTELPAKDQRPPLRFMIGIDTETFHQTQHQLLIALVSLAVIGVLLASALGYWV
CCCHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARIGLKPLIKLSHEAQRLAPPLRAGRLRLSPLPPELEQFVESFNSTLERVEQAYSRLESF
HHHCHHHHHHHHHHHHHCCCCHHHCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
NADVAHELRSPLTNLIGQTQVALTRGRSAEHYFEVLQSNLEELERLRSIINDMLFLASAD
CHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QGSKATKLTSTSLADEVATTLEYLDFILEDAQVQVRVSGDARVQIEIAHLRRALINLLSN
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEHHHHHHHHHHHHHH
AVQHTAPGQVIEVKIAAEEHQVSIGVANPGLPIASEHLPRLFERFYRVDASRSNSGNNHG
HHHHCCCCCEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
LGLAIVKAIALMHGGDVFVRSDRGMNTFGIHLPV
HHHHHHHHHHHHCCCCEEEECCCCCCEEEEEECC
>Mature Secondary Structure 
SSNSIALRLSGMFTLVALLVFLLIGGALYQQVDKGLGLLPEAELDARYSVLESALNRYG
CCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCC
TPEHWVKINAKLKLLGEEDKRIRFWVVSGNPGYEFGEPDALIRAFAQGPLGMRDLQLPDH
CCCCEEEEEEEEEEECCCCCEEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
PYPLKVLLTELPAKDQRPPLRFMIGIDTETFHQTQHQLLIALVSLAVIGVLLASALGYWV
CCCHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARIGLKPLIKLSHEAQRLAPPLRAGRLRLSPLPPELEQFVESFNSTLERVEQAYSRLESF
HHHCHHHHHHHHHHHHHCCCCHHHCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
NADVAHELRSPLTNLIGQTQVALTRGRSAEHYFEVLQSNLEELERLRSIINDMLFLASAD
CHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QGSKATKLTSTSLADEVATTLEYLDFILEDAQVQVRVSGDARVQIEIAHLRRALINLLSN
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEHHHHHHHHHHHHHH
AVQHTAPGQVIEVKIAAEEHQVSIGVANPGLPIASEHLPRLFERFYRVDASRSNSGNNHG
HHHHCCCCCEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
LGLAIVKAIALMHGGDVFVRSDRGMNTFGIHLPV
HHHHHHHHHHHHCCCCEEEECCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]