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The map label for this gene is dctD [H]

Identifier: 77460679

GI number: 77460679

Start: 5029815

End: 5031143

Strand: Direct

Name: dctD [H]

Synonym: Pfl01_4458

Alternate gene names: 77460679

Gene position: 5029815-5031143 (Clockwise)

Preceding gene: 77460678

Following gene: 77460680

Centisome position: 78.12

GC content: 64.48

Gene sequence:

>1329_bases
ATGCTCAATTCCGTGATGGTGGTGGATGACGAAAGCAGCATTCGCAGTGCCGTCGAGCAATGGCTGAGCCTGTCCGGGTT
CGAGGTGCAGCTGTTCAGCCGCGCCGAGGAATGCCTGGCGGCGCTGCCGGCGCATTTTGCCGGGGTGATTGTCAGCGACG
TGCGCATGCCCGGCCTCAGCGGTCTTGAACTGCTGGCCGAAGTGCAGCGCCGGGATGCCGACCTGCCGGTGATTCTGCTG
ACCGGCCACGGCGACGTACCGATGGCGGTCGAAGCGATGCGCGACGGCGCCTACGATTTTCTGGAAAAACCGTTCAGCCC
CGAGACCCTGCTCGGCAGCCTGCGCCGGGCGCTGGACAAGCGTCGGCTGGTGCTGGAAAACCGCGCCCTGCACGAGCAGG
CCGACAACCGCGCCAGACTCGATGCGACACTGCTCGGCGTGTCCCGTGGCTTGCAGACCTTGCGCCGGCAGGTACTTGAT
CTGGCGACGCTGCCGGTCAATGTGCTGATCCGTGGCGAAACCGGCAGCGGCAAGGAGCTGGTCGCCCGCTGCCTGCACGA
CTTCGGCCCGCGCGCCGACAAGCCGTTCGTGGCGCTGAACTGCGCGGCGATCCCCGAGCAGTTGTTCGAAGCCGAGTTGT
TCGGGCATGAGAGCGGCGCGTTCACCGGCGCCTCGGGCAAGCGCATCGGCAAGCTGGAATACGCCGATGGCGGCACGCTG
TTTCTCGATGAAATCGAAAGCATGCCGCTGGCCCAGCAGGTGAAACTGCTGCGGGTGTTGCAGGAGCAGAAGCTGGAGCG
GCTGGGGTCGAACCAGAGCATTCGGGTGGACTTGCGGATTGTCGCGGCGACCAAACCCGACTTGCTGGATGAAGCGCGGG
CCGGGCGTTTTCGCGAGGACCTGGCGTATCGGCTGAACGTCGCAGAGCTGCGATTGCCACCGCTGCGTGATCGTCGGGAA
GACATTCCGCTGTTGTTCGAATCCTTCGCCCAGAGTGCCGCCCAGCGTTTGGGCCGGACCTTTCCGCCGCTCAGTGGCGC
GCAGTTGAGCCACCTGCTCAGTCACGACTGGCCGGGCAATGTGCGCGAGTTGGCGAACGTGGCCGAGCGGCAGGTGCTGG
GGCTGGATGAACCGGTGCAGGGTATCGATCCGGGCCAGTCGCTGGCGGCGCAGCAAGAGGCGTTTGAGGCGCAGTGTTTG
CGGGCTGCTTTGACCCGGCACAAGGGCGATGTGAAAGCCGTGCTGGAGGAACTGCAACTGCCGCGTCGTACGTTTAATGA
AAAGATGCAGCGCCATGGTTTGACCCGGGAAATGTTTATTCCCGATTGA

Upstream 100 bases:

>100_bases
CCATCGTCCATGAATCCGGCGGGCGTCTGTGCGTCGAAAATGGTGACAATGGCGCAGTGTTCAGCCTGACCTTGCCGATC
GATCTGGAGGCGCACATCTG

Downstream 100 bases:

>100_bases
TCCTTCTTGATTGATCGTTCCCACGCTCCGCGTGGGAACGATCAGATAGGCGGAAATCCGCTCATCGCCAAGTTCCTATC
GGCAAAATCCCGCTCAACCC

Product: two component, sigma-54 specific, Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MLNSVMVVDDESSIRSAVEQWLSLSGFEVQLFSRAEECLAALPAHFAGVIVSDVRMPGLSGLELLAEVQRRDADLPVILL
TGHGDVPMAVEAMRDGAYDFLEKPFSPETLLGSLRRALDKRRLVLENRALHEQADNRARLDATLLGVSRGLQTLRRQVLD
LATLPVNVLIRGETGSGKELVARCLHDFGPRADKPFVALNCAAIPEQLFEAELFGHESGAFTGASGKRIGKLEYADGGTL
FLDEIESMPLAQQVKLLRVLQEQKLERLGSNQSIRVDLRIVAATKPDLLDEARAGRFREDLAYRLNVAELRLPPLRDRRE
DIPLLFESFAQSAAQRLGRTFPPLSGAQLSHLLSHDWPGNVRELANVAERQVLGLDEPVQGIDPGQSLAAQQEAFEAQCL
RAALTRHKGDVKAVLEELQLPRRTFNEKMQRHGLTREMFIPD

Sequences:

>Translated_442_residues
MLNSVMVVDDESSIRSAVEQWLSLSGFEVQLFSRAEECLAALPAHFAGVIVSDVRMPGLSGLELLAEVQRRDADLPVILL
TGHGDVPMAVEAMRDGAYDFLEKPFSPETLLGSLRRALDKRRLVLENRALHEQADNRARLDATLLGVSRGLQTLRRQVLD
LATLPVNVLIRGETGSGKELVARCLHDFGPRADKPFVALNCAAIPEQLFEAELFGHESGAFTGASGKRIGKLEYADGGTL
FLDEIESMPLAQQVKLLRVLQEQKLERLGSNQSIRVDLRIVAATKPDLLDEARAGRFREDLAYRLNVAELRLPPLRDRRE
DIPLLFESFAQSAAQRLGRTFPPLSGAQLSHLLSHDWPGNVRELANVAERQVLGLDEPVQGIDPGQSLAAQQEAFEAQCL
RAALTRHKGDVKAVLEELQLPRRTFNEKMQRHGLTREMFIPD
>Mature_442_residues
MLNSVMVVDDESSIRSAVEQWLSLSGFEVQLFSRAEECLAALPAHFAGVIVSDVRMPGLSGLELLAEVQRRDADLPVILL
TGHGDVPMAVEAMRDGAYDFLEKPFSPETLLGSLRRALDKRRLVLENRALHEQADNRARLDATLLGVSRGLQTLRRQVLD
LATLPVNVLIRGETGSGKELVARCLHDFGPRADKPFVALNCAAIPEQLFEAELFGHESGAFTGASGKRIGKLEYADGGTL
FLDEIESMPLAQQVKLLRVLQEQKLERLGSNQSIRVDLRIVAATKPDLLDEARAGRFREDLAYRLNVAELRLPPLRDRRE
DIPLLFESFAQSAAQRLGRTFPPLSGAQLSHLLSHDWPGNVRELANVAERQVLGLDEPVQGIDPGQSLAAQQEAFEAQCL
RAALTRHKGDVKAVLEELQLPRRTFNEKMQRHGLTREMFIPD

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. When activated by dctB acts in conjunction with sigma-54 to activate the transcription of dctA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=378, Percent_Identity=43.6507936507937, Blast_Score=305, Evalue=3e-84,
Organism=Escherichia coli, GI1790299, Length=367, Percent_Identity=45.2316076294278, Blast_Score=301, Evalue=8e-83,
Organism=Escherichia coli, GI1790437, Length=440, Percent_Identity=38.4090909090909, Blast_Score=284, Evalue=1e-77,
Organism=Escherichia coli, GI1788905, Length=447, Percent_Identity=38.7024608501119, Blast_Score=278, Evalue=7e-76,
Organism=Escherichia coli, GI1789087, Length=336, Percent_Identity=44.3452380952381, Blast_Score=251, Evalue=6e-68,
Organism=Escherichia coli, GI87082117, Length=248, Percent_Identity=52.0161290322581, Blast_Score=242, Evalue=4e-65,
Organism=Escherichia coli, GI1789233, Length=321, Percent_Identity=39.8753894080997, Blast_Score=222, Evalue=5e-59,
Organism=Escherichia coli, GI87082152, Length=230, Percent_Identity=49.5652173913044, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1786524, Length=352, Percent_Identity=41.1931818181818, Blast_Score=211, Evalue=6e-56,
Organism=Escherichia coli, GI87081872, Length=236, Percent_Identity=46.6101694915254, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1787583, Length=235, Percent_Identity=42.5531914893617, Blast_Score=177, Evalue=8e-46,
Organism=Escherichia coli, GI87081858, Length=289, Percent_Identity=30.7958477508651, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1789828, Length=215, Percent_Identity=34.8837209302326, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1789809, Length=118, Percent_Identity=28.8135593220339, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788222, Length=131, Percent_Identity=28.2442748091603, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49016; Mature: 49016

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNSVMVVDDESSIRSAVEQWLSLSGFEVQLFSRAEECLAALPAHFAGVIVSDVRMPGLS
CCCCEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
GLELLAEVQRRDADLPVILLTGHGDVPMAVEAMRDGAYDFLEKPFSPETLLGSLRRALDK
HHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHH
RRLVLENRALHEQADNRARLDATLLGVSRGLQTLRRQVLDLATLPVNVLIRGETGSGKEL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHH
VARCLHDFGPRADKPFVALNCAAIPEQLFEAELFGHESGAFTGASGKRIGKLEYADGGTL
HHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCEECCCCCCCCEEEECCCCEE
FLDEIESMPLAQQVKLLRVLQEQKLERLGSNQSIRVDLRIVAATKPDLLDEARAGRFRED
EHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHHCCHHHHH
LAYRLNVAELRLPPLRDRREDIPLLFESFAQSAAQRLGRTFPPLSGAQLSHLLSHDWPGN
HHHHCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCC
VRELANVAERQVLGLDEPVQGIDPGQSLAAQQEAFEAQCLRAALTRHKGDVKAVLEELQL
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
PRRTFNEKMQRHGLTREMFIPD
CHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLNSVMVVDDESSIRSAVEQWLSLSGFEVQLFSRAEECLAALPAHFAGVIVSDVRMPGLS
CCCCEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
GLELLAEVQRRDADLPVILLTGHGDVPMAVEAMRDGAYDFLEKPFSPETLLGSLRRALDK
HHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHH
RRLVLENRALHEQADNRARLDATLLGVSRGLQTLRRQVLDLATLPVNVLIRGETGSGKEL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHH
VARCLHDFGPRADKPFVALNCAAIPEQLFEAELFGHESGAFTGASGKRIGKLEYADGGTL
HHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCEECCCCCCCCEEEECCCCEE
FLDEIESMPLAQQVKLLRVLQEQKLERLGSNQSIRVDLRIVAATKPDLLDEARAGRFRED
EHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHHCCHHHHH
LAYRLNVAELRLPPLRDRREDIPLLFESFAQSAAQRLGRTFPPLSGAQLSHLLSHDWPGN
HHHHCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCC
VRELANVAERQVLGLDEPVQGIDPGQSLAAQQEAFEAQCLRAALTRHKGDVKAVLEELQL
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
PRRTFNEKMQRHGLTREMFIPD
CHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431 [H]