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The map label for this gene is kgtP [H]

Identifier: 77460680

GI number: 77460680

Start: 5031358

End: 5032677

Strand: Direct

Name: kgtP [H]

Synonym: Pfl01_4459

Alternate gene names: 77460680

Gene position: 5031358-5032677 (Clockwise)

Preceding gene: 77460679

Following gene: 77460682

Centisome position: 78.15

GC content: 60.38

Gene sequence:

>1320_bases
ATGGATAACTCCAACGCCCTGCCTCTTGGGTCGGCTGCCGTGCCGGCCAAAGAACGAACCACCGCCAGTCGCATCAAGTC
GATCTTCAGCGGTTCCGTCGGCAACATGGTCGAGTGGTACGACTGGTACGTCTATGCCGCCTTCTCGCTGTATTTCGCCA
AAGCCTTCTTCCCCAAAGGCGACACCACCGCCCAACTGCTCAACACCGCCGCGATCTTCGCCGTGGGCTTCCTGATGCGC
CCGATCGGTGGCTGGCTGATGGGGCTGTACGCCGACAAGGTCGGACGCAAAAAAGCCCTGATGGCCTCGGTTTACCTGAT
GTGCTTCGGCTCGCTGATCATCGCCCTGAGCCCGGGCTATGAAACCATCGGTGTCGGCGCGCCGATCCTGCTGGTGTTCG
CCCGCCTGCTGCAAGGCCTGTCGGTCGGTGGCGAATACGGCACCTCGGCGACCTACCTGTCGGAGATGGCGACCAAGGAT
CGTCGCGGCTTCTTCTCCAGCTTCCAGTACGTGACTTTGATTTCCGGCCAGCTCATCGCGCTGGGCGTGCTGATCGTGCT
GCAACAGTTCCTGACCACCGAAGAGCTTTACGCGTGGGGCTGGCGTATTCCGTTCGCCATCGGTGCGCTGTGCGCAATCG
TGGCGCTGTACCTGCGTCGTGGCATGGAAGAAACCGAGTCGTTCACCAAGAAAGAGAAGTCCAAGGAAAGCGCCATGCGC
ACCTTGCTTCGTCATCCGAAGGAACTGCTGACCGTGGTCGGCCTGACCATGGGCGGTACGCTGGCGTTCTACACCTACAC
CACCTACATGCAGAAATACCTGGTGAACACCGTCGGCATGAGCATCTCCGACTCCACCACCATTTCCGCCGCCACGCTGT
TCCTGTTCATGTGCCTGCAACCGATCATCGGCGGCCTGTCGGATAAAATCGGTCGTCGCCCGATCCTGATCGCCTTCGGC
GTGCTCGGTACGATCTTCACCGTGCCGATCCTGATGACCCTGCACACCATCCAGACCTGGTGGGGCGCGTTCTTCCTGAT
CATGGCGGCGCTGATCATTGTCAGCGGCTACACCTCGATCAACGCCGTGGTGAAGGCCGAGCTGTTCCCGACCGAAATCC
GCGCCCTGGGCGTCGGCCTGCCGTACGCACTGACCGTGTCGATCTTCGGCGGCACCGCCGAATACATCGCGCTGTGGTTC
AAGAGCATCGGCATGGAAACCGGTTATTACTGGTACGTGACCGCGTGCATCGCGGTGTCCCTGCTGGTGTACATCACCAT
GAAAGACACGCAGAAACATTCTCGGATCGTCACTGACTGA

Upstream 100 bases:

>100_bases
TCTAAACCGGCCCTCCCCCCGTTGGCACAGCTCCTGCTATAGCCCTCGCAGGCTGCGTTCCAACGCGCTCCACAAAAACA
ATTAAACGAAGGATCCTTCA

Downstream 100 bases:

>100_bases
TCACAAAAAAACGCGCGAAGGTGCAAGACCTTCGCGCGTTTTCTTTCCTGTCCGATTTCCCGATCAGGACAATTCGGCAA
CGCCCTGTTTGCTGCCATAG

Product: citrate-proton symport

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MDNSNALPLGSAAVPAKERTTASRIKSIFSGSVGNMVEWYDWYVYAAFSLYFAKAFFPKGDTTAQLLNTAAIFAVGFLMR
PIGGWLMGLYADKVGRKKALMASVYLMCFGSLIIALSPGYETIGVGAPILLVFARLLQGLSVGGEYGTSATYLSEMATKD
RRGFFSSFQYVTLISGQLIALGVLIVLQQFLTTEELYAWGWRIPFAIGALCAIVALYLRRGMEETESFTKKEKSKESAMR
TLLRHPKELLTVVGLTMGGTLAFYTYTTYMQKYLVNTVGMSISDSTTISAATLFLFMCLQPIIGGLSDKIGRRPILIAFG
VLGTIFTVPILMTLHTIQTWWGAFFLIMAALIIVSGYTSINAVVKAELFPTEIRALGVGLPYALTVSIFGGTAEYIALWF
KSIGMETGYYWYVTACIAVSLLVYITMKDTQKHSRIVTD

Sequences:

>Translated_439_residues
MDNSNALPLGSAAVPAKERTTASRIKSIFSGSVGNMVEWYDWYVYAAFSLYFAKAFFPKGDTTAQLLNTAAIFAVGFLMR
PIGGWLMGLYADKVGRKKALMASVYLMCFGSLIIALSPGYETIGVGAPILLVFARLLQGLSVGGEYGTSATYLSEMATKD
RRGFFSSFQYVTLISGQLIALGVLIVLQQFLTTEELYAWGWRIPFAIGALCAIVALYLRRGMEETESFTKKEKSKESAMR
TLLRHPKELLTVVGLTMGGTLAFYTYTTYMQKYLVNTVGMSISDSTTISAATLFLFMCLQPIIGGLSDKIGRRPILIAFG
VLGTIFTVPILMTLHTIQTWWGAFFLIMAALIIVSGYTSINAVVKAELFPTEIRALGVGLPYALTVSIFGGTAEYIALWF
KSIGMETGYYWYVTACIAVSLLVYITMKDTQKHSRIVTD
>Mature_439_residues
MDNSNALPLGSAAVPAKERTTASRIKSIFSGSVGNMVEWYDWYVYAAFSLYFAKAFFPKGDTTAQLLNTAAIFAVGFLMR
PIGGWLMGLYADKVGRKKALMASVYLMCFGSLIIALSPGYETIGVGAPILLVFARLLQGLSVGGEYGTSATYLSEMATKD
RRGFFSSFQYVTLISGQLIALGVLIVLQQFLTTEELYAWGWRIPFAIGALCAIVALYLRRGMEETESFTKKEKSKESAMR
TLLRHPKELLTVVGLTMGGTLAFYTYTTYMQKYLVNTVGMSISDSTTISAATLFLFMCLQPIIGGLSDKIGRRPILIAFG
VLGTIFTVPILMTLHTIQTWWGAFFLIMAALIIVSGYTSINAVVKAELFPTEIRALGVGLPYALTVSIFGGTAEYIALWF
KSIGMETGYYWYVTACIAVSLLVYITMKDTQKHSRIVTD

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=432, Percent_Identity=55.5555555555556, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI1790550, Length=425, Percent_Identity=32.2352941176471, Blast_Score=222, Evalue=4e-59,
Organism=Escherichia coli, GI1788292, Length=428, Percent_Identity=31.5420560747664, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1789941, Length=427, Percent_Identity=31.615925058548, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI87082404, Length=379, Percent_Identity=25.065963060686, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI87082231, Length=262, Percent_Identity=28.2442748091603, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 48188; Mature: 48188

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNSNALPLGSAAVPAKERTTASRIKSIFSGSVGNMVEWYDWYVYAAFSLYFAKAFFPKG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
DTTAQLLNTAAIFAVGFLMRPIGGWLMGLYADKVGRKKALMASVYLMCFGSLIIALSPGY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCC
ETIGVGAPILLVFARLLQGLSVGGEYGTSATYLSEMATKDRRGFFSSFQYVTLISGQLIA
CCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LGVLIVLQQFLTTEELYAWGWRIPFAIGALCAIVALYLRRGMEETESFTKKEKSKESAMR
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
TLLRHPKELLTVVGLTMGGTLAFYTYTTYMQKYLVNTVGMSISDSTTISAATLFLFMCLQ
HHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
PIIGGLSDKIGRRPILIAFGVLGTIFTVPILMTLHTIQTWWGAFFLIMAALIIVSGYTSI
HHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
NAVVKAELFPTEIRALGVGLPYALTVSIFGGTAEYIALWFKSIGMETGYYWYVTACIAVS
HHHHHHHHHHHHHHHHCCCCCHHEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LLVYITMKDTQKHSRIVTD
HHHHHHCCCCHHCCCCCCC
>Mature Secondary Structure
MDNSNALPLGSAAVPAKERTTASRIKSIFSGSVGNMVEWYDWYVYAAFSLYFAKAFFPKG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
DTTAQLLNTAAIFAVGFLMRPIGGWLMGLYADKVGRKKALMASVYLMCFGSLIIALSPGY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCC
ETIGVGAPILLVFARLLQGLSVGGEYGTSATYLSEMATKDRRGFFSSFQYVTLISGQLIA
CCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LGVLIVLQQFLTTEELYAWGWRIPFAIGALCAIVALYLRRGMEETESFTKKEKSKESAMR
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
TLLRHPKELLTVVGLTMGGTLAFYTYTTYMQKYLVNTVGMSISDSTTISAATLFLFMCLQ
HHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
PIIGGLSDKIGRRPILIAFGVLGTIFTVPILMTLHTIQTWWGAFFLIMAALIIVSGYTSI
HHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
NAVVKAELFPTEIRALGVGLPYALTVSIFGGTAEYIALWFKSIGMETGYYWYVTACIAVS
HHHHHHHHHHHHHHHHCCCCCHHEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LLVYITMKDTQKHSRIVTD
HHHHHHCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]