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The map label for this gene is queC

Identifier: 77460619

GI number: 77460619

Start: 4959678

End: 4960370

Strand: Reverse

Name: queC

Synonym: Pfl01_4398

Alternate gene names: 77460619

Gene position: 4960370-4959678 (Counterclockwise)

Preceding gene: 77460620

Following gene: 77460618

Centisome position: 77.04

GC content: 61.04

Gene sequence:

>693_bases
ATGACTGAACAACTGAACACTAGCCAAAAACGTGCGGTAATCCTGCTGTCGGGTGGTCTGGACTCGGCTACGGTGGTGGC
CATGGCCCGCGCTGAAGGCTACGCCTGCTACACCATGAGTTTCGATTACGGTCAGCGTTCCCACGCCGAACTGCATGCCG
CTGCGCGCGTTGCCCGCGATCTGGGTGTGGTCGAGCACAAGGTGATCGGCCTGAACCTGAACGGCATGGGCGGCTCGGCC
CTGACCGACACCAGCATCGACATCCCGGAAGAGCTGGGCGAAGGCATCCCGGTGACTTACGTGCCGGCACGCAACACGGT
ATTCCTGTCGCTGGCTCTCGGCTGGGCTGAAGTGCTCGGCGCCCGTGACATCTTTATCGGCGTCAACGCAGTGGATTACT
CCGGTTACCCGGATTGCCGTCCCGAGTTCATCGAGTCGTTCGAGCGCATGGCCAATCTGGCGACCAAGGCTGGCGTCGAA
GGTAATGGTTTCCGCATCCAGGCACCGCTGCAGAACTTGAGCAAGGCGCAGATCGTCCAGGCGGGCGTGAAGCTTGGCGT
TGATTACGGGCTGACCGTTTCCTGCTATCAGGCTGACGATGACGGCCGTGCTTGCGGTAAATGCGACAGCTGCCGACTGC
GTGCAGAAGGCTTCGCGGCGGCCGGAATCAGCGACCCGACACGTTATTTTTGA

Upstream 100 bases:

>100_bases
GCTCGGGATCTGGCGGACTGGGTGGTGGCGGATAACCTGCCAGTGCGCCTGCAACTGCAGCTGCATAAATATCTATGGAA
TGATGAGCCGGGGCGCTGAG

Downstream 100 bases:

>100_bases
TTTTTTTCAAATTAGGTGTTGAATAGTCCTTAAAAATCAGTATTATACGCGCCACCACACAGCGGGTCGTTAGCTCAGTT
GGTAGAGCAGTTGGCTTTTA

Product: ExsB

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine synthase; PreQ(0) synthase; Queuosine biosynthesis protein queC

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MTEQLNTSQKRAVILLSGGLDSATVVAMARAEGYACYTMSFDYGQRSHAELHAAARVARDLGVVEHKVIGLNLNGMGGSA
LTDTSIDIPEELGEGIPVTYVPARNTVFLSLALGWAEVLGARDIFIGVNAVDYSGYPDCRPEFIESFERMANLATKAGVE
GNGFRIQAPLQNLSKAQIVQAGVKLGVDYGLTVSCYQADDDGRACGKCDSCRLRAEGFAAAGISDPTRYF

Sequences:

>Translated_230_residues
MTEQLNTSQKRAVILLSGGLDSATVVAMARAEGYACYTMSFDYGQRSHAELHAAARVARDLGVVEHKVIGLNLNGMGGSA
LTDTSIDIPEELGEGIPVTYVPARNTVFLSLALGWAEVLGARDIFIGVNAVDYSGYPDCRPEFIESFERMANLATKAGVE
GNGFRIQAPLQNLSKAQIVQAGVKLGVDYGLTVSCYQADDDGRACGKCDSCRLRAEGFAAAGISDPTRYF
>Mature_229_residues
TEQLNTSQKRAVILLSGGLDSATVVAMARAEGYACYTMSFDYGQRSHAELHAAARVARDLGVVEHKVIGLNLNGMGGSAL
TDTSIDIPEELGEGIPVTYVPARNTVFLSLALGWAEVLGARDIFIGVNAVDYSGYPDCRPEFIESFERMANLATKAGVEG
NGFRIQAPLQNLSKAQIVQAGVKLGVDYGLTVSCYQADDDGRACGKCDSCRLRAEGFAAAGISDPTRYF

Specific function: Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0))

COG id: COG0603

COG function: function code R; Predicted PP-loop superfamily ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queC family

Homologues:

Organism=Escherichia coli, GI1786648, Length=224, Percent_Identity=37.0535714285714, Blast_Score=135, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEC_PSEPF (Q3K7W9)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_350126.1
- ProteinModelPortal:   Q3K7W9
- SMR:   Q3K7W9
- STRING:   Q3K7W9
- GeneID:   3712842
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_4398
- eggNOG:   COG0603
- HOGENOM:   HBG553284
- OMA:   GWAEVLG
- ProtClustDB:   CLSK866256
- BioCyc:   PFLU205922:PFL_4398-MONOMER
- HAMAP:   MF_01633_B
- InterPro:   IPR018317
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF006293
- TIGRFAMs:   TIGR00364

Pfam domain/function: PF06508 ExsB

EC number: NA

Molecular weight: Translated: 24511; Mature: 24380

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQLNTSQKRAVILLSGGLDSATVVAMARAEGYACYTMSFDYGQRSHAELHAAARVARD
CCCCCCCCCCEEEEEEECCCCCEEEEEEEECCCEEEEEEEECCCCCHHHHHHHHHHHHHH
LGVVEHKVIGLNLNGMGGSALTDTSIDIPEELGEGIPVTYVPARNTVFLSLALGWAEVLG
HCCHHEEEEEEEECCCCCCCCCCCCCCCHHHHCCCCCEEEECCCCEEEEEEHHHHHHHHC
ARDIFIGVNAVDYSGYPDCRPEFIESFERMANLATKAGVEGNGFRIQAPLQNLSKAQIVQ
CCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHCCHHHHHHH
AGVKLGVDYGLTVSCYQADDDGRACGKCDSCRLRAEGFAAAGISDPTRYF
HHHHCCCCCCCEEEEEECCCCCCCCCCCCCCEEECCCEEECCCCCCCCCC
>Mature Secondary Structure 
TEQLNTSQKRAVILLSGGLDSATVVAMARAEGYACYTMSFDYGQRSHAELHAAARVARD
CCCCCCCCCEEEEEEECCCCCEEEEEEEECCCEEEEEEEECCCCCHHHHHHHHHHHHHH
LGVVEHKVIGLNLNGMGGSALTDTSIDIPEELGEGIPVTYVPARNTVFLSLALGWAEVLG
HCCHHEEEEEEEECCCCCCCCCCCCCCCHHHHCCCCCEEEECCCCEEEEEEHHHHHHHHC
ARDIFIGVNAVDYSGYPDCRPEFIESFERMANLATKAGVEGNGFRIQAPLQNLSKAQIVQ
CCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHCCHHHHHHH
AGVKLGVDYGLTVSCYQADDDGRACGKCDSCRLRAEGFAAAGISDPTRYF
HHHHCCCCCCCEEEEEECCCCCCCCCCCCCCEEECCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA