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The map label for this gene is argT [H]

Identifier: 77460513

GI number: 77460513

Start: 4848049

End: 4848822

Strand: Reverse

Name: argT [H]

Synonym: Pfl01_4292

Alternate gene names: 77460513

Gene position: 4848822-4848049 (Counterclockwise)

Preceding gene: 77460514

Following gene: 77460512

Centisome position: 75.31

GC content: 59.17

Gene sequence:

>774_bases
ATGAAGAAACTTGTGCTGCTTGGCGCCCTGGCACTGTCCGTGCTGTCCCTGCCGACATTCGCCGATGAAAAGCCTCTGAA
AATCGGTATCGAAGCGGCTTACCCTCCATTCGCCTCCAAAGCGCCGGACGGCAGCATCGTCGGTTTCGACTACGACATCG
GCAACGCGCTGTGCGAAGAGATGAAGGTCAAGTGCCAGTGGGTCGAGCAAGAGTTCGACGGTCTGATCCCGGCACTGAAA
GTGCGCAAGATCGACGCGATCCTGTCGTCCATGTCGATCACTGACGACCGCAAGAAGTCCGTGGACTTCACCAACAAGTA
CTACAACACCCCGGCCCGCCTCGTGATGAAGGAAGGCACTCAGGTCAGCGAAGGTCTGGCTGAGCTCAAGGGCAAGAACA
TCGGCGTGCAACGTGGTTCGATCCACGAGCGTTTCGCCCGCGAAGTCCTGGCCCCGCTGGGTGCCGAGATCAAGCCTTAT
GGTTCGCAGAACGAAATCTACCTCGACGTGGCCGCCGGCCGCCTCGACGGCACCGTGGCGGACGCTACGCTGTTGAATGA
CGGCTTCCTGAAAACCGACGCCGGCAAAGGCTTCGCGTTCGTGGGTCCGGCGTTCACCGACGTCAAATACTTCGGCGACG
GCGTAGGCATCGCGGTACGCAAGGGCGACGCCCTGAAAGACAAGATCAACACCGCCATCGCGGCCATCCGCGAGAACGGC
AAGTACAAGGCAATCCAGGACAAGTACTTCGACTTCGACATTTACGGCAAGTAA

Upstream 100 bases:

>100_bases
AATTGTCCCACGCACGCAGCAACCGATTTCGTTACACCCATTCGCATATTGGGCTGTCGCTCACTCTGCCGTTTTTGCCC
TTTACCGATGGAGTCCCAAG

Downstream 100 bases:

>100_bases
AGACGTCGCTCGACCCGTCCGAAATGGCGCAAGCAACAGGATCTCTGCGGTTTGCGCCATTTTTTCATCCCAACTTTCGA
GGACCTGAATCATGTTGAAA

Product: lysine-arginine-ornithine-binding periplasmic protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MKKLVLLGALALSVLSLPTFADEKPLKIGIEAAYPPFASKAPDGSIVGFDYDIGNALCEEMKVKCQWVEQEFDGLIPALK
VRKIDAILSSMSITDDRKKSVDFTNKYYNTPARLVMKEGTQVSEGLAELKGKNIGVQRGSIHERFAREVLAPLGAEIKPY
GSQNEIYLDVAAGRLDGTVADATLLNDGFLKTDAGKGFAFVGPAFTDVKYFGDGVGIAVRKGDALKDKINTAIAAIRENG
KYKAIQDKYFDFDIYGK

Sequences:

>Translated_257_residues
MKKLVLLGALALSVLSLPTFADEKPLKIGIEAAYPPFASKAPDGSIVGFDYDIGNALCEEMKVKCQWVEQEFDGLIPALK
VRKIDAILSSMSITDDRKKSVDFTNKYYNTPARLVMKEGTQVSEGLAELKGKNIGVQRGSIHERFAREVLAPLGAEIKPY
GSQNEIYLDVAAGRLDGTVADATLLNDGFLKTDAGKGFAFVGPAFTDVKYFGDGVGIAVRKGDALKDKINTAIAAIRENG
KYKAIQDKYFDFDIYGK
>Mature_257_residues
MKKLVLLGALALSVLSLPTFADEKPLKIGIEAAYPPFASKAPDGSIVGFDYDIGNALCEEMKVKCQWVEQEFDGLIPALK
VRKIDAILSSMSITDDRKKSVDFTNKYYNTPARLVMKEGTQVSEGLAELKGKNIGVQRGSIHERFAREVLAPLGAEIKPY
GSQNEIYLDVAAGRLDGTVADATLLNDGFLKTDAGKGFAFVGPAFTDVKYFGDGVGIAVRKGDALKDKINTAIAAIRENG
KYKAIQDKYFDFDIYGK

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=254, Percent_Identity=44.8818897637795, Blast_Score=232, Evalue=2e-62,
Organism=Escherichia coli, GI1788648, Length=232, Percent_Identity=43.1034482758621, Blast_Score=207, Evalue=8e-55,
Organism=Escherichia coli, GI1787085, Length=253, Percent_Identity=40.7114624505929, Blast_Score=178, Evalue=3e-46,
Organism=Escherichia coli, GI1787088, Length=251, Percent_Identity=39.8406374501992, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1788228, Length=263, Percent_Identity=28.8973384030418, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1787031, Length=248, Percent_Identity=31.0483870967742, Blast_Score=117, Evalue=6e-28,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27943; Mature: 27943

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLVLLGALALSVLSLPTFADEKPLKIGIEAAYPPFASKAPDGSIVGFDYDIGNALCEE
CCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCHHHHHHHH
MKVKCQWVEQEFDGLIPALKVRKIDAILSSMSITDDRKKSVDFTNKYYNTPARLVMKEGT
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHCCHHHEEEECCC
QVSEGLAELKGKNIGVQRGSIHERFAREVLAPLGAEIKPYGSQNEIYLDVAAGRLDGTVA
CHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCHH
DATLLNDGFLKTDAGKGFAFVGPAFTDVKYFGDGVGIAVRKGDALKDKINTAIAAIRENG
HHEEECCCCEEECCCCCEEEECCCHHHHHECCCCEEEEEECCCHHHHHHHHHHHHHHCCC
KYKAIQDKYFDFDIYGK
CEEEECCCCCCEEECCC
>Mature Secondary Structure
MKKLVLLGALALSVLSLPTFADEKPLKIGIEAAYPPFASKAPDGSIVGFDYDIGNALCEE
CCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCHHHHHHHH
MKVKCQWVEQEFDGLIPALKVRKIDAILSSMSITDDRKKSVDFTNKYYNTPARLVMKEGT
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHCCHHHEEEECCC
QVSEGLAELKGKNIGVQRGSIHERFAREVLAPLGAEIKPYGSQNEIYLDVAAGRLDGTVA
CHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCHH
DATLLNDGFLKTDAGKGFAFVGPAFTDVKYFGDGVGIAVRKGDALKDKINTAIAAIRENG
HHEEECCCCEEECCCCCEEEECCCHHHHHECCCCEEEEEECCCHHHHHHHHHHHHHHCCC
KYKAIQDKYFDFDIYGK
CEEEECCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]