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The map label for this gene is lysC [H]

Identifier: 77460495

GI number: 77460495

Start: 4821551

End: 4822792

Strand: Reverse

Name: lysC [H]

Synonym: Pfl01_4274

Alternate gene names: 77460495

Gene position: 4822792-4821551 (Counterclockwise)

Preceding gene: 77460496

Following gene: 77460494

Centisome position: 74.91

GC content: 58.94

Gene sequence:

>1242_bases
ATGGCTTTGATCGTACAGAAATTTGGAGGCACCTCGGTCGGCACTGTCGAGAGAATCGAGCAGGTCGCCGACAAGGTTAA
GAAATTCCGCGATGCCGGCGATGACCTGGTGGTTGTGCTGTCTGCAATGAGCGGCGAAACCAACCGTCTGATCGATCTGG
CCAAGCAAATCAGTGGCGAAGATCAACCGGTTCCGCGCGAACTGGATGTGATCGTTTCCACAGGTGAGCAGGTGACGATC
GCCCTGTTGGCCATGGCGCTGATCAAGCGCGGTGTGCCGGCGGTGTCGTACACCGGCAACCAGGTGCGGATTCTGACGGA
CAGTGCGCACAATAAAGCGCGTATCTTGCAGATTGATGACCAGAAGATTCGCGGTGACCTGAAAGCCGGTCGCGTGGTGG
TGGTCGCTGGTTTCCAGGGCGTCGACGAGCACGGCAACATCACGACCCTGGGTCGTGGCGGTTCCGACACCACCGGTGTG
GCGCTGGCCGCTGCGCTGAAGGCTGACGAGTGCCAGATCTACACCGACGTCGATGGTGTCTACACCACTGACCCGCGTGT
GGTATCGGTTGCTCAGCGTCTGGACAAGATCACCTTCGAAGAGATGCTGGAAATGGCCAGCCTCGGTTCCAAGGTGCTGC
AGATCCGTGCGGTGGAATTCGCCGGCAAGTACAACGTTCCGCTGCGCGTACTGCACAGCTTCAAGGAGGGTCCGGGCACC
CTCATTACTATTGATGAAGAGGAAACCATGGAACAGCCGATCATTTCCGGCATCGCTTTCAACCGCGATGAAGCCAAGCT
GACCATCCGTGGCGTGCCAGACACCCCGGGCGTGGCGTTCAAGATTCTCGGCCCGATCAGTGCCGCGAACATCGAAGTCG
ACATGATCGTGCAGAACGTCGCGCACGATAACACCACCGACTTCACCTTCACCGTGCACCGCAACGACTACCAGTCCGCG
CAAACCGTGCTGGAAAACACCGCTCGCGAGATCGGTGCCCGTGAAGTGGTGGGCGATACCAAGATTGCCAAGGTCTCGAT
CGTCGGCGTCGGCATGCGTTCCCACGCAGGCGTGGCCAGCCGCATGTTTGAATCCCTGGCAAAAGAAAGCATCAACATCC
AGATGATCTCGACCTCGGAAATCAAGGTTTCCGTGGTGATCGAAGAGAAGTACCTGGAACTGGCCGTGCGCGCCCTGCAC
ACGGCTTTCGAACTCGACGCTCCGGCCCGTCAGGGCGAGTGA

Upstream 100 bases:

>100_bases
GGGTTTATAAGACGGCCCGTCGGGCCCGCAGTCTAGTGGCGGACCCGGCGGCTGTTCGAGTGATTATTGGGCGCCCTTCA
TGGGCAGAGGCGGCTTTGAA

Downstream 100 bases:

>100_bases
TGCGTTGTCTGAAGGGCGCGGTCTGACCGCGCCCTTTATTTTTTGAATGGCGTGAGCCCTGAACTGTTCTTTTGCTCGCG
CTGGTCAATACTCAGGCATG

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MALIVQKFGGTSVGTVERIEQVADKVKKFRDAGDDLVVVLSAMSGETNRLIDLAKQISGEDQPVPRELDVIVSTGEQVTI
ALLAMALIKRGVPAVSYTGNQVRILTDSAHNKARILQIDDQKIRGDLKAGRVVVVAGFQGVDEHGNITTLGRGGSDTTGV
ALAAALKADECQIYTDVDGVYTTDPRVVSVAQRLDKITFEEMLEMASLGSKVLQIRAVEFAGKYNVPLRVLHSFKEGPGT
LITIDEEETMEQPIISGIAFNRDEAKLTIRGVPDTPGVAFKILGPISAANIEVDMIVQNVAHDNTTDFTFTVHRNDYQSA
QTVLENTAREIGAREVVGDTKIAKVSIVGVGMRSHAGVASRMFESLAKESINIQMISTSEIKVSVVIEEKYLELAVRALH
TAFELDAPARQGE

Sequences:

>Translated_413_residues
MALIVQKFGGTSVGTVERIEQVADKVKKFRDAGDDLVVVLSAMSGETNRLIDLAKQISGEDQPVPRELDVIVSTGEQVTI
ALLAMALIKRGVPAVSYTGNQVRILTDSAHNKARILQIDDQKIRGDLKAGRVVVVAGFQGVDEHGNITTLGRGGSDTTGV
ALAAALKADECQIYTDVDGVYTTDPRVVSVAQRLDKITFEEMLEMASLGSKVLQIRAVEFAGKYNVPLRVLHSFKEGPGT
LITIDEEETMEQPIISGIAFNRDEAKLTIRGVPDTPGVAFKILGPISAANIEVDMIVQNVAHDNTTDFTFTVHRNDYQSA
QTVLENTAREIGAREVVGDTKIAKVSIVGVGMRSHAGVASRMFESLAKESINIQMISTSEIKVSVVIEEKYLELAVRALH
TAFELDAPARQGE
>Mature_412_residues
ALIVQKFGGTSVGTVERIEQVADKVKKFRDAGDDLVVVLSAMSGETNRLIDLAKQISGEDQPVPRELDVIVSTGEQVTIA
LLAMALIKRGVPAVSYTGNQVRILTDSAHNKARILQIDDQKIRGDLKAGRVVVVAGFQGVDEHGNITTLGRGGSDTTGVA
LAAALKADECQIYTDVDGVYTTDPRVVSVAQRLDKITFEEMLEMASLGSKVLQIRAVEFAGKYNVPLRVLHSFKEGPGTL
ITIDEEETMEQPIISGIAFNRDEAKLTIRGVPDTPGVAFKILGPISAANIEVDMIVQNVAHDNTTDFTFTVHRNDYQSAQ
TVLENTAREIGAREVVGDTKIAKVSIVGVGMRSHAGVASRMFESLAKESINIQMISTSEIKVSVVIEEKYLELAVRALHT
AFELDAPARQGE

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=467, Percent_Identity=29.5503211991435, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1790455, Length=275, Percent_Identity=31.2727272727273, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790376, Length=350, Percent_Identity=23.1428571428571, Blast_Score=65, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6320893, Length=490, Percent_Identity=26.3265306122449, Blast_Score=108, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 44597; Mature: 44465

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALIVQKFGGTSVGTVERIEQVADKVKKFRDAGDDLVVVLSAMSGETNRLIDLAKQISGE
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCC
DQPVPRELDVIVSTGEQVTIALLAMALIKRGVPAVSYTGNQVRILTDSAHNKARILQIDD
CCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCCEEEEEECC
QKIRGDLKAGRVVVVAGFQGVDEHGNITTLGRGGSDTTGVALAAALKADECQIYTDVDGV
HHHCCCCCCCCEEEEECCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCEEEEECCCCE
YTTDPRVVSVAQRLDKITFEEMLEMASLGSKVLQIRAVEFAGKYNVPLRVLHSFKEGPGT
EECCCHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHCCCCE
LITIDEEETMEQPIISGIAFNRDEAKLTIRGVPDTPGVAFKILGPISAANIEVDMIVQNV
EEEECCHHHHHHHHHHCEEECCCCCEEEEECCCCCCCEEEEEECCCCCCCEEHHHHHHHH
AHDNTTDFTFTVHRNDYQSAQTVLENTAREIGAREVVGDTKIAKVSIVGVGMRSHAGVAS
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCHHHCCCCEEEEEEEEEECCCCCCHHHH
RMFESLAKESINIQMISTSEIKVSVVIEEKYLELAVRALHTAFELDAPARQGE
HHHHHHHHCCCEEEEEEECCEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ALIVQKFGGTSVGTVERIEQVADKVKKFRDAGDDLVVVLSAMSGETNRLIDLAKQISGE
EEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCC
DQPVPRELDVIVSTGEQVTIALLAMALIKRGVPAVSYTGNQVRILTDSAHNKARILQIDD
CCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCCEEEEEECC
QKIRGDLKAGRVVVVAGFQGVDEHGNITTLGRGGSDTTGVALAAALKADECQIYTDVDGV
HHHCCCCCCCCEEEEECCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCEEEEECCCCE
YTTDPRVVSVAQRLDKITFEEMLEMASLGSKVLQIRAVEFAGKYNVPLRVLHSFKEGPGT
EECCCHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHCCCCE
LITIDEEETMEQPIISGIAFNRDEAKLTIRGVPDTPGVAFKILGPISAANIEVDMIVQNV
EEEECCHHHHHHHHHHCEEECCCCCEEEEECCCCCCCEEEEEECCCCCCCEEHHHHHHHH
AHDNTTDFTFTVHRNDYQSAQTVLENTAREIGAREVVGDTKIAKVSIVGVGMRSHAGVAS
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCHHHCCCCEEEEEEEEEECCCCCCHHHH
RMFESLAKESINIQMISTSEIKVSVVIEEKYLELAVRALHTAFELDAPARQGE
HHHHHHHHCCCEEEEEEECCEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]