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The map label for this gene is dgoD [H]

Identifier: 77460344

GI number: 77460344

Start: 4665208

End: 4666356

Strand: Reverse

Name: dgoD [H]

Synonym: Pfl01_4123

Alternate gene names: 77460344

Gene position: 4666356-4665208 (Counterclockwise)

Preceding gene: 77460345

Following gene: 77460343

Centisome position: 72.48

GC content: 61.62

Gene sequence:

>1149_bases
ATGAAAATCACCAAACTCACCACCTTCATCGTCCCGCCGCGCTGGTGCTTCCTGAAAGTCGAAACCGACGAAGGCGTGAC
CGGTTGGGGCGAGCCCGTGGTCGAGGGCCGCGCCCACACGGTCGCTGCTGCGGTTGAGGAATTGTCCGACTACCTGATCG
GCAAAGACCCACGCAATATCGAAGACATCTGGACCGTGCTGTATCGCGGTGGCTTCTACCGAGGCGGCGCGATCCACATG
AGCGCGCTGGCCGGTATCGATCAGGCGCTGTGGGACATCAAGGGCAAGGCCCTCGGTGTATCGGTCAGCGATCTGCTCGG
CGGTCAGGTGCGGGACAAGATCCGCGTGTATTCGTGGATCGGCGGCGACCGCCCGGCGGACACTGCGCGGGCCGCGAAAG
AGGCGGTGGGCCGAGGTTTTACGGCGGTGAAAATGAACGGCACCGAGGAACTGCAATTCCTCGATACCTTCGAAAAGGTC
GATCTGGCCCTGGCCAACGTTGCCGCCGTGCGCGATGCGGTCGGGCCGAACGTCGGCATCGGCGTCGACTTCCATGGCCG
GGTGCACAAACCCATGGCCAAGGTGCTGATGAAGGAACTCGACCCGTACAAACTGATGTTCATCGAAGAGCCGGTGCTCA
GCGAAAACTACGAAGCGCTGAAAGAACTGGCTCCGCTGACCAGCACCCCGATTGCCCTCGGTGAGCGGCTGTTTTCGCGC
TGGGACTTTAAACGGGTGTTGAGCGAAGGTTACGTCGACATCATCCAGCCGGACGCGTCCCACGCCGGCGGCATCACCGA
GACCCGCAAGATCGCCAACATGGCCGAAGCCTACGACGTGGCGCTGGCACTGCATTGCCCGCTGGGGCCGATTGCACTGG
CGGCGTGTCTGCAACTGGACGCCGCTTGTTACAACGCGTTTATCCAGGAGCAGAGCCTGGGCATCCATTACAACGAGAGC
AATGACTTGCTCGATTATGTGAAGGATCCGCGGGTGTTCGACTACGACAAGGGCTTCGTGAAAATCCCGAACGGCCCGGG
CCTGGGGATCGAGATCAACGAGGAATACGTGATCGAACGCGCGGCGGTCGGTCATCGCTGGCGCAACCCGATCTGGCGGC
ATGCCGATGGCAGTTTTGCCGAGTGGTGA

Upstream 100 bases:

>100_bases
AGTGGCGACCAATGCCAAGGCCTACGTGGCGGCCTGGAAAGCCCTTCGCTAAGACTTTTCCGGCGCTGCGTGCGCTGCAT
CTAACAAGAGAGACAAGCAG

Downstream 100 bases:

>100_bases
GTCGCTGACTCTCTGCCGCATCCAGTAAGTCAGATCTCCAGGGAGCGGAGATCTGCATAACCCTCGACCTCAATAAACAT
AAAAAGAGGCGCTCCCCATG

Product: galactonate dehydratase

Products: NA

Alternate protein names: GalD [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MKITKLTTFIVPPRWCFLKVETDEGVTGWGEPVVEGRAHTVAAAVEELSDYLIGKDPRNIEDIWTVLYRGGFYRGGAIHM
SALAGIDQALWDIKGKALGVSVSDLLGGQVRDKIRVYSWIGGDRPADTARAAKEAVGRGFTAVKMNGTEELQFLDTFEKV
DLALANVAAVRDAVGPNVGIGVDFHGRVHKPMAKVLMKELDPYKLMFIEEPVLSENYEALKELAPLTSTPIALGERLFSR
WDFKRVLSEGYVDIIQPDASHAGGITETRKIANMAEAYDVALALHCPLGPIALAACLQLDAACYNAFIQEQSLGIHYNES
NDLLDYVKDPRVFDYDKGFVKIPNGPGLGIEINEEYVIERAAVGHRWRNPIWRHADGSFAEW

Sequences:

>Translated_382_residues
MKITKLTTFIVPPRWCFLKVETDEGVTGWGEPVVEGRAHTVAAAVEELSDYLIGKDPRNIEDIWTVLYRGGFYRGGAIHM
SALAGIDQALWDIKGKALGVSVSDLLGGQVRDKIRVYSWIGGDRPADTARAAKEAVGRGFTAVKMNGTEELQFLDTFEKV
DLALANVAAVRDAVGPNVGIGVDFHGRVHKPMAKVLMKELDPYKLMFIEEPVLSENYEALKELAPLTSTPIALGERLFSR
WDFKRVLSEGYVDIIQPDASHAGGITETRKIANMAEAYDVALALHCPLGPIALAACLQLDAACYNAFIQEQSLGIHYNES
NDLLDYVKDPRVFDYDKGFVKIPNGPGLGIEINEEYVIERAAVGHRWRNPIWRHADGSFAEW
>Mature_382_residues
MKITKLTTFIVPPRWCFLKVETDEGVTGWGEPVVEGRAHTVAAAVEELSDYLIGKDPRNIEDIWTVLYRGGFYRGGAIHM
SALAGIDQALWDIKGKALGVSVSDLLGGQVRDKIRVYSWIGGDRPADTARAAKEAVGRGFTAVKMNGTEELQFLDTFEKV
DLALANVAAVRDAVGPNVGIGVDFHGRVHKPMAKVLMKELDPYKLMFIEEPVLSENYEALKELAPLTSTPIALGERLFSR
WDFKRVLSEGYVDIIQPDASHAGGITETRKIANMAEAYDVALALHCPLGPIALAACLQLDAACYNAFIQEQSLGIHYNES
NDLLDYVKDPRVFDYDKGFVKIPNGPGLGIEINEEYVIERAAVGHRWRNPIWRHADGSFAEW

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=382, Percent_Identity=62.0418848167539, Blast_Score=485, Evalue=1e-138,
Organism=Escherichia coli, GI1787864, Length=417, Percent_Identity=32.1342925659472, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI226510960, Length=266, Percent_Identity=31.5789473684211, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 42216; Mature: 42216

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00908 MR_MLE_1 ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITKLTTFIVPPRWCFLKVETDEGVTGWGEPVVEGRAHTVAAAVEELSDYLIGKDPRNI
CCCEEEEEEEECCCEEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCCCCH
EDIWTVLYRGGFYRGGAIHMSALAGIDQALWDIKGKALGVSVSDLLGGQVRDKIRVYSWI
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEECEEHHHHHCCHHHHHEEEEEEC
GGDRPADTARAAKEAVGRGFTAVKMNGTEELQFLDTFEKVDLALANVAAVRDAVGPNVGI
CCCCCCHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
GVDFHGRVHKPMAKVLMKELDPYKLMFIEEPVLSENYEALKELAPLTSTPIALGERLFSR
EECCCCCHHHHHHHHHHHHCCCEEEEEEECCHHCCCHHHHHHHCCCCCCHHHHHHHHHHH
WDFKRVLSEGYVDIIQPDASHAGGITETRKIANMAEAYDVALALHCPLGPIALAACLQLD
HHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHH
AACYNAFIQEQSLGIHYNESNDLLDYVKDPRVFDYDKGFVKIPNGPGLGIEINEEYVIER
HHHHHHHHHHCCCCEEECCCCCHHHHHCCCCEEECCCCEEECCCCCCCCEEECHHHHHHH
AAVGHRWRNPIWRHADGSFAEW
HHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKITKLTTFIVPPRWCFLKVETDEGVTGWGEPVVEGRAHTVAAAVEELSDYLIGKDPRNI
CCCEEEEEEEECCCEEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCCCCH
EDIWTVLYRGGFYRGGAIHMSALAGIDQALWDIKGKALGVSVSDLLGGQVRDKIRVYSWI
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEECEEHHHHHCCHHHHHEEEEEEC
GGDRPADTARAAKEAVGRGFTAVKMNGTEELQFLDTFEKVDLALANVAAVRDAVGPNVGI
CCCCCCHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
GVDFHGRVHKPMAKVLMKELDPYKLMFIEEPVLSENYEALKELAPLTSTPIALGERLFSR
EECCCCCHHHHHHHHHHHHCCCEEEEEEECCHHCCCHHHHHHHCCCCCCHHHHHHHHHHH
WDFKRVLSEGYVDIIQPDASHAGGITETRKIANMAEAYDVALALHCPLGPIALAACLQLD
HHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHH
AACYNAFIQEQSLGIHYNESNDLLDYVKDPRVFDYDKGFVKIPNGPGLGIEINEEYVIER
HHHHHHHHHHCCCCEEECCCCCHHHHHCCCCEEECCCCEEECCCCCCCCEEECHHHHHHH
AAVGHRWRNPIWRHADGSFAEW
HHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA