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The map label for this gene is gap [H]

Identifier: 77460086

GI number: 77460086

Start: 4367043

End: 4368506

Strand: Reverse

Name: gap [H]

Synonym: Pfl01_3865

Alternate gene names: 77460086

Gene position: 4368506-4367043 (Counterclockwise)

Preceding gene: 77460090

Following gene: 77460084

Centisome position: 67.85

GC content: 59.08

Gene sequence:

>1464_bases
ATGTGGAAGGTTACCGTGACTCAGAAGCCCGACCAGTGTCTTGGTGAATGGATCGACCGTGAAGCACTCGCAGAAGCGAT
GATTCCGCTTATCGGTCAGCTCTACCGCAACAACAACGTGGTGAGCTCGATCTATGGCCGCAGCCTGATCAACCAGTCTG
TCATCGCGATTCTCAAAGCTCACCGCTTTGCTCGCCACCGTTCTGCCGACGACAGCGAACTCTCCGTCCACGAAACATTC
CCACTGCTCAAGGCCATGAGCGAGCTCAAGCTCGGCGCGGCCTCGGTAGATCTGGGCAAACTGGCGTACAAATTCCGCAA
CGAAGGCAACGGCCGCACCGCCGAGCAGTTCGTCCGCGAAGAAATGGCTGACGTGGTTGGCCAGCAAAACGCTTCGGCCC
GCAAAGGCACCGACGTTGTTCTGTACGGCTTCGGTCGTATCGGCCGTCTGCTGGCGCGCATCCTGATCGAGAAAACCGGT
GGCGGCGACGGCCTGCGTCTGCGTGCCATCGTGGTGCGCAAGGGCGCCGAAAACGACCTGACCAAGCGCGCCAGCCTGCT
GCGTCGCGATTCGGTACATGGTTCGTTCAACGGCACCATCACCATCGACGAAGAAAACAACACCATCACCGCCAACGGCA
ACCTGATCCAGGTTATCTACGCGAAGAACCCTACCGAAGTGGATTACACCCAGTACGGCATCAAAGATGCGCTGCTGGTG
GACAACACCGGTGTATGGCGTGATGCCGATGGCCTGGGCCAGCACCTGGCGTGCCCGGGTATCGACCGCGTTGTTCTGAC
TGCGCCTGGCAAGGGCAAGCTGAAGAACATCGTTCACGGTATCAACCACAACGAAATCACTGCTGACGACAAGATCGTGT
CCGCCGCTTCCTGCACCACCAACGCCATCGTGCCGGTGCTGAAAGCCGTGAATGACAAGTTCGGCATCATCAACGGTCAC
GTCGAAACCGTTCACTCGTACACCAACGACCAGAACCTGATCGACAACTTCCACAAGGGCGATCGCCGTGGCCGTAGCGC
CGCGCTGAACATGGTGATCACCGAGACCGGTGCTGCCACCGCTGCTGCCAAGGCCCTGCCTGAGCTGGCCGGCAAGCTGA
CCGGTAACGCGATCCGTGTTCCGACGCCGAACGTGTCGATGGCCATTCTCAACCTGAACCTCGAGAAAGCCGCCACCCGT
GAAGAGATGAACGAGTACCTGCGCTACATGGCGCTGCACTCCGATCTGCACAAGCAAATCGACTTCGTCAATTCGCAGGA
AGTGGTTTCGACCGACTTCGTGGGCTCGCGTCACGCCGGTGTGGTCGACGCTGAAGCGACCATCGTTCAGGACAACCGCG
TTGTTCTGTACGTCTGGTACGACAACGAGTTCGGTTACAGCTGCCAGGTGGTTCGCGTAATGGAAGACATGGCCGGTGTA
AACCCGCCAGCGTTCCCGCGCTAA

Upstream 100 bases:

>100_bases
TATTTTTTTGGACGGATTTTTGTTACCGATTGCGCGACAAGCGCGCATTGCTACAAGAGGTTCTCGGCGGCATAATGTAG
CCCCTTTTTTCTGCCCCTAC

Downstream 100 bases:

>100_bases
GCCTTAGCTGCAAATGAAAACGCCCCGACTCTGGTCGGGGCGTTTTTGTTTGTGCGCGATTTGCCTTAGCGGGGGATGGC
GGCTTGTGCCGTGCGCAGTT

Product: glyceraldehyde-3-phosphate dehydrogenase

Products: NA

Alternate protein names: GAPDH [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MWKVTVTQKPDQCLGEWIDREALAEAMIPLIGQLYRNNNVVSSIYGRSLINQSVIAILKAHRFARHRSADDSELSVHETF
PLLKAMSELKLGAASVDLGKLAYKFRNEGNGRTAEQFVREEMADVVGQQNASARKGTDVVLYGFGRIGRLLARILIEKTG
GGDGLRLRAIVVRKGAENDLTKRASLLRRDSVHGSFNGTITIDEENNTITANGNLIQVIYAKNPTEVDYTQYGIKDALLV
DNTGVWRDADGLGQHLACPGIDRVVLTAPGKGKLKNIVHGINHNEITADDKIVSAASCTTNAIVPVLKAVNDKFGIINGH
VETVHSYTNDQNLIDNFHKGDRRGRSAALNMVITETGAATAAAKALPELAGKLTGNAIRVPTPNVSMAILNLNLEKAATR
EEMNEYLRYMALHSDLHKQIDFVNSQEVVSTDFVGSRHAGVVDAEATIVQDNRVVLYVWYDNEFGYSCQVVRVMEDMAGV
NPPAFPR

Sequences:

>Translated_487_residues
MWKVTVTQKPDQCLGEWIDREALAEAMIPLIGQLYRNNNVVSSIYGRSLINQSVIAILKAHRFARHRSADDSELSVHETF
PLLKAMSELKLGAASVDLGKLAYKFRNEGNGRTAEQFVREEMADVVGQQNASARKGTDVVLYGFGRIGRLLARILIEKTG
GGDGLRLRAIVVRKGAENDLTKRASLLRRDSVHGSFNGTITIDEENNTITANGNLIQVIYAKNPTEVDYTQYGIKDALLV
DNTGVWRDADGLGQHLACPGIDRVVLTAPGKGKLKNIVHGINHNEITADDKIVSAASCTTNAIVPVLKAVNDKFGIINGH
VETVHSYTNDQNLIDNFHKGDRRGRSAALNMVITETGAATAAAKALPELAGKLTGNAIRVPTPNVSMAILNLNLEKAATR
EEMNEYLRYMALHSDLHKQIDFVNSQEVVSTDFVGSRHAGVVDAEATIVQDNRVVLYVWYDNEFGYSCQVVRVMEDMAGV
NPPAFPR
>Mature_487_residues
MWKVTVTQKPDQCLGEWIDREALAEAMIPLIGQLYRNNNVVSSIYGRSLINQSVIAILKAHRFARHRSADDSELSVHETF
PLLKAMSELKLGAASVDLGKLAYKFRNEGNGRTAEQFVREEMADVVGQQNASARKGTDVVLYGFGRIGRLLARILIEKTG
GGDGLRLRAIVVRKGAENDLTKRASLLRRDSVHGSFNGTITIDEENNTITANGNLIQVIYAKNPTEVDYTQYGIKDALLV
DNTGVWRDADGLGQHLACPGIDRVVLTAPGKGKLKNIVHGINHNEITADDKIVSAASCTTNAIVPVLKAVNDKFGIINGH
VETVHSYTNDQNLIDNFHKGDRRGRSAALNMVITETGAATAAAKALPELAGKLTGNAIRVPTPNVSMAILNLNLEKAATR
EEMNEYLRYMALHSDLHKQIDFVNSQEVVSTDFVGSRHAGVVDAEATIVQDNRVVLYVWYDNEFGYSCQVVRVMEDMAGV
NPPAFPR

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7669492, Length=338, Percent_Identity=38.7573964497041, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI7657116, Length=338, Percent_Identity=36.9822485207101, Blast_Score=194, Evalue=2e-49,
Organism=Escherichia coli, GI1788079, Length=337, Percent_Identity=38.5756676557863, Blast_Score=198, Evalue=7e-52,
Organism=Escherichia coli, GI1789295, Length=344, Percent_Identity=31.3953488372093, Blast_Score=165, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17534677, Length=346, Percent_Identity=38.150289017341, Blast_Score=197, Evalue=8e-51,
Organism=Caenorhabditis elegans, GI17534679, Length=346, Percent_Identity=37.5722543352601, Blast_Score=195, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI17568413, Length=347, Percent_Identity=37.7521613832853, Blast_Score=188, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI32566163, Length=347, Percent_Identity=37.7521613832853, Blast_Score=188, Evalue=7e-48,
Organism=Saccharomyces cerevisiae, GI6322409, Length=343, Percent_Identity=38.7755102040816, Blast_Score=207, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6322468, Length=343, Percent_Identity=37.0262390670554, Blast_Score=197, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6321631, Length=346, Percent_Identity=37.5722543352601, Blast_Score=197, Evalue=3e-51,
Organism=Drosophila melanogaster, GI85725000, Length=337, Percent_Identity=38.2789317507418, Blast_Score=198, Evalue=8e-51,
Organism=Drosophila melanogaster, GI22023983, Length=337, Percent_Identity=38.2789317507418, Blast_Score=198, Evalue=8e-51,
Organism=Drosophila melanogaster, GI17933600, Length=337, Percent_Identity=37.9821958456973, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI18110149, Length=337, Percent_Identity=37.9821958456973, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI19922412, Length=332, Percent_Identity=38.2530120481928, Blast_Score=189, Evalue=3e-48,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 53342; Mature: 53342

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWKVTVTQKPDQCLGEWIDREALAEAMIPLIGQLYRNNNVVSSIYGRSLINQSVIAILKA
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
HRFARHRSADDSELSVHETFPLLKAMSELKLGAASVDLGKLAYKFRNEGNGRTAEQFVRE
HHHHHHCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHH
EMADVVGQQNASARKGTDVVLYGFGRIGRLLARILIEKTGGGDGLRLRAIVVRKGAENDL
HHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCHH
TKRASLLRRDSVHGSFNGTITIDEENNTITANGNLIQVIYAKNPTEVDYTQYGIKDALLV
HHHHHHHHHHCCCCCCCCEEEEECCCCEEEECCCEEEEEEECCCCCCCHHHHCCCCEEEE
DNTGVWRDADGLGQHLACPGIDRVVLTAPGKGKLKNIVHGINHNEITADDKIVSAASCTT
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHCCCCCCCCCCCCCEEHHCCCH
NAIVPVLKAVNDKFGIINGHVETVHSYTNDQNLIDNFHKGDRRGRSAALNMVITETGAAT
HHHHHHHHHHCCCCCEECCHHHHHHHHCCCHHHHHHHHCCCCCCCHHEEEEEEECCCCHH
AAAKALPELAGKLTGNAIRVPTPNVSMAILNLNLEKAATREEMNEYLRYMALHSDLHKQI
HHHHHHHHHHHHHCCCEEECCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
DFVNSQEVVSTDFVGSRHAGVVDAEATIVQDNRVVLYVWYDNEFGYSCQVVRVMEDMAGV
HCCCCCHHHHHCCCCCCCCCEEECCEEEEECCEEEEEEEECCCCCCCCHHHHHHHHHHCC
NPPAFPR
CCCCCCC
>Mature Secondary Structure
MWKVTVTQKPDQCLGEWIDREALAEAMIPLIGQLYRNNNVVSSIYGRSLINQSVIAILKA
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
HRFARHRSADDSELSVHETFPLLKAMSELKLGAASVDLGKLAYKFRNEGNGRTAEQFVRE
HHHHHHCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHH
EMADVVGQQNASARKGTDVVLYGFGRIGRLLARILIEKTGGGDGLRLRAIVVRKGAENDL
HHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCHH
TKRASLLRRDSVHGSFNGTITIDEENNTITANGNLIQVIYAKNPTEVDYTQYGIKDALLV
HHHHHHHHHHCCCCCCCCEEEEECCCCEEEECCCEEEEEEECCCCCCCHHHHCCCCEEEE
DNTGVWRDADGLGQHLACPGIDRVVLTAPGKGKLKNIVHGINHNEITADDKIVSAASCTT
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHCCCCCCCCCCCCCEEHHCCCH
NAIVPVLKAVNDKFGIINGHVETVHSYTNDQNLIDNFHKGDRRGRSAALNMVITETGAAT
HHHHHHHHHHCCCCCEECCHHHHHHHHCCCHHHHHHHHCCCCCCCHHEEEEEEECCCCHH
AAAKALPELAGKLTGNAIRVPTPNVSMAILNLNLEKAATREEMNEYLRYMALHSDLHKQI
HHHHHHHHHHHHHCCCEEECCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
DFVNSQEVVSTDFVGSRHAGVVDAEATIVQDNRVVLYVWYDNEFGYSCQVVRVMEDMAGV
HCCCCCHHHHHCCCCCCCCCEEECCEEEEECCEEEEEEEECCCCCCCCHHHHHHHHHHCC
NPPAFPR
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3680173 [H]