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The map label for this gene is apbE [H]

Identifier: 77460084

GI number: 77460084

Start: 4364582

End: 4365631

Strand: Reverse

Name: apbE [H]

Synonym: Pfl01_3863

Alternate gene names: 77460084

Gene position: 4365631-4364582 (Counterclockwise)

Preceding gene: 77460086

Following gene: 77460083

Centisome position: 67.81

GC content: 59.14

Gene sequence:

>1050_bases
ATGGCCGGCGCGGTTTTGCGGGGAGATGAAGAATTGTTAACTGGACGGTGGGGGATTCTCGTGCTGCTGGGCTGGGTTTT
GGCCGGCTGTGGCAACGGCGATTCCCTTGAGACTTTCGGTGGCCCGACGATGGGCAGCACCTATTCGATCAAGTACGTGC
GCCACGCCCATCTGCCTGCCCCCCAGACGGTGCGTATCGAAGTGGAAAAAATCCTCGCGGACGTCGACCGGCAGATGTCG
ACTTACCGCGCAGACTCCGATATCTCGCGCTTCAATGCATTGCCGGCTGATCGCTGTCAGGTCATGCCCGCACCGATCCT
TGAATTGATCCGCGTGGGCGAACAGCTGTCGCAACAAAGCGAAGGCTCCTACGACCTCACGGTTGAGCCGCTGATGAATC
TGTGGGGTTTCGGCCCGCAGGGGCGTGAAGAAAAAGTCCCGGATGCGGCGGCGCTGGCCAAAGTGCGCCAACGAGTCGGT
CATCAACACCTGCACATCGACGGCGACCGGTTATGCAAGGATGCGGCGGTCGAGGTGGACGTCAACAGCATTGCCGCCGG
GTACGCGGTTGACACCATTACCGCGCGGCTCGAGGCTCTGGGCATCCATGACTATCTCGCGGAAGCCACCGGCGAGCTCA
AGGCTTCCGGCAAGAAGCTTGATGGTTCGCCGTGGCGTATCGCTCTGGAAGAACCGCAAGACGATCAGCAGGTGGCAGAG
CGCGTCATTAATGTGGATGGCTACGGCCTGTCCACCTCCGGCGACTATCGCAACTATTTTCAGCAGGATGGCCGTCGCTA
TTCCCACACCTTCGATGCCCGGACCGGTGCGCCGGTCCTACACGATCTGGCGTCGGTCACGGTAATTCATCCTTCAGCGC
TGATGGCCGATGGACTATCGACGCTGTTGCTGATTCTCGGGCCTGAAAGAGCTTGGGACTATGCCGAAAAACACGACATT
GGTGCATTCTTTGTGATTCGTGCCGATACAGGTTTCGTCACACGCACCAGTCACGCTTTTGAACGGCTCAGTGGCGCAAA
AGTTGACTGA

Upstream 100 bases:

>100_bases
CTGACAACGCGAAAAATCGCCTTGCCTTTGCCGGTCGTTGAGGACATGGGTAGGTACTCACTCTAAATCTTGTAAGAAAG
CGTTGCGCTCGTAGCCCATA

Downstream 100 bases:

>100_bases
GAACAGTACGAAAGCTGGCGTTGTAGTGCAGGCAAAAGTAGCCTACGACGCGACCAAGGGTTAATGTGCGCGGCGTTGAC
GCTTCTATAGACTGTGTCCG

Product: ApbE-like lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MAGAVLRGDEELLTGRWGILVLLGWVLAGCGNGDSLETFGGPTMGSTYSIKYVRHAHLPAPQTVRIEVEKILADVDRQMS
TYRADSDISRFNALPADRCQVMPAPILELIRVGEQLSQQSEGSYDLTVEPLMNLWGFGPQGREEKVPDAAALAKVRQRVG
HQHLHIDGDRLCKDAAVEVDVNSIAAGYAVDTITARLEALGIHDYLAEATGELKASGKKLDGSPWRIALEEPQDDQQVAE
RVINVDGYGLSTSGDYRNYFQQDGRRYSHTFDARTGAPVLHDLASVTVIHPSALMADGLSTLLLILGPERAWDYAEKHDI
GAFFVIRADTGFVTRTSHAFERLSGAKVD

Sequences:

>Translated_349_residues
MAGAVLRGDEELLTGRWGILVLLGWVLAGCGNGDSLETFGGPTMGSTYSIKYVRHAHLPAPQTVRIEVEKILADVDRQMS
TYRADSDISRFNALPADRCQVMPAPILELIRVGEQLSQQSEGSYDLTVEPLMNLWGFGPQGREEKVPDAAALAKVRQRVG
HQHLHIDGDRLCKDAAVEVDVNSIAAGYAVDTITARLEALGIHDYLAEATGELKASGKKLDGSPWRIALEEPQDDQQVAE
RVINVDGYGLSTSGDYRNYFQQDGRRYSHTFDARTGAPVLHDLASVTVIHPSALMADGLSTLLLILGPERAWDYAEKHDI
GAFFVIRADTGFVTRTSHAFERLSGAKVD
>Mature_348_residues
AGAVLRGDEELLTGRWGILVLLGWVLAGCGNGDSLETFGGPTMGSTYSIKYVRHAHLPAPQTVRIEVEKILADVDRQMST
YRADSDISRFNALPADRCQVMPAPILELIRVGEQLSQQSEGSYDLTVEPLMNLWGFGPQGREEKVPDAAALAKVRQRVGH
QHLHIDGDRLCKDAAVEVDVNSIAAGYAVDTITARLEALGIHDYLAEATGELKASGKKLDGSPWRIALEEPQDDQQVAER
VINVDGYGLSTSGDYRNYFQQDGRRYSHTFDARTGAPVLHDLASVTVIHPSALMADGLSTLLLILGPERAWDYAEKHDIG
AFFVIRADTGFVTRTSHAFERLSGAKVD

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=277, Percent_Identity=35.3790613718412, Blast_Score=195, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 38125; Mature: 37994

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGAVLRGDEELLTGRWGILVLLGWVLAGCGNGDSLETFGGPTMGSTYSIKYVRHAHLPA
CCCCEECCCHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCC
PQTVRIEVEKILADVDRQMSTYRADSDISRFNALPADRCQVMPAPILELIRVGEQLSQQS
CCEEEEEHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHC
EGSYDLTVEPLMNLWGFGPQGREEKVPDAAALAKVRQRVGHQHLHIDGDRLCKDAAVEVD
CCCCEEEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECHHHHHHHCEEEEE
VNSIAAGYAVDTITARLEALGIHDYLAEATGELKASGKKLDGSPWRIALEEPQDDQQVAE
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHH
RVINVDGYGLSTSGDYRNYFQQDGRRYSHTFDARTGAPVLHDLASVTVIHPSALMADGLS
HHHCCCCCCCCCCCHHHHHHHHCCCEECEECCCCCCCHHHHHHHHHEEECCHHHHHHHHH
TLLLILGPERAWDYAEKHDIGAFFVIRADTGFVTRTSHAFERLSGAKVD
HEEHEECCCHHHCCHHHCCCCEEEEEEECCCCEEEHHHHHHHHCCCCCC
>Mature Secondary Structure 
AGAVLRGDEELLTGRWGILVLLGWVLAGCGNGDSLETFGGPTMGSTYSIKYVRHAHLPA
CCCEECCCHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCC
PQTVRIEVEKILADVDRQMSTYRADSDISRFNALPADRCQVMPAPILELIRVGEQLSQQS
CCEEEEEHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHC
EGSYDLTVEPLMNLWGFGPQGREEKVPDAAALAKVRQRVGHQHLHIDGDRLCKDAAVEVD
CCCCEEEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECHHHHHHHCEEEEE
VNSIAAGYAVDTITARLEALGIHDYLAEATGELKASGKKLDGSPWRIALEEPQDDQQVAE
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHH
RVINVDGYGLSTSGDYRNYFQQDGRRYSHTFDARTGAPVLHDLASVTVIHPSALMADGLS
HHHCCCCCCCCCCCHHHHHHHHCCCEECEECCCCCCCHHHHHHHHHEEECCHHHHHHHHH
TLLLILGPERAWDYAEKHDIGAFFVIRADTGFVTRTSHAFERLSGAKVD
HEEHEECCCHHHCCHHHCCCCEEEEEEECCCCEEEHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]