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The map label for this gene is emrA [H]

Identifier: 77459670

GI number: 77459670

Start: 3931980

End: 3933122

Strand: Reverse

Name: emrA [H]

Synonym: Pfl01_3448

Alternate gene names: 77459670

Gene position: 3933122-3931980 (Counterclockwise)

Preceding gene: 77459671

Following gene: 77459666

Centisome position: 61.09

GC content: 63.43

Gene sequence:

>1143_bases
ATGACCGAACCGACCACCACAACCACCAATGCCATCGCCGCCACCCCTGAAGGCGTGGCGCCGCCGTCATCACCGAATAC
CGAGCCGCGGTCGTTGCGGGTGCGGATCATCTCGTCGCTGGGCTTTGCCGGGGTCGCCATCGTCGGTGTGCTGATCGTGC
TCTATGCCTGGCAGTTGCCGCCGTTCAGCAGCGCGGTGGAGACCACTGAAAACGCCCTGGTGCGCGGGCAGGTGACGATC
ATCGGCCCGCAGCTCAGTGGCTATGTGTTTGAGGTGCCGGTACAGGACTTTCAGTTTGTGAAGGAGGGCGATTTGCTGGT
ACGTCTCGATGACCGCATCTATCAGCAACGCCTCGACCAGTCGCTGGCACAACTGGCGGTGCAGAAGGCTTCGCTGGCCA
ACGTGGTGCAGCAGCGCAACAGCGCCGAAGCCACGATAAAACTGCGGCAGGCGGTGGTGGCCGACAGCGAAGCGCAGTTG
CGCAAAAGCGAGGCTGACTTGCGACGCAACAAAGCGCTGGTCAATGACGGCTCCGTGTCCAAACGCGAGATGGACGTAGC
GCTGGCCGCCAACGCGCAAAGCATTGCAGCGGTGGCGCAGGCCAAGGCCAATCTGGAAATCGCCCGGCAGGATCTGCAAA
CGGTGATCGTCAATCGCGGTTCGCTGGAGGCGGCGGTGGCCAGTGCCGAGGCGGCGGTGCAGTTGGCGCGGATCGACTTG
TCCAACACCCGGATCATCGCCCCGCGTGACGGACAGCTCGGGCAGATCGGCGTGCGCCTCGGCGCCTACGTCAACTCTGG
GGCGCAGTTGATGGCGCTGGTGCCGAACCAGAAATGGGTAATCGCCAACCTCAAGGAGACCCAGATGGACAACGTGCGCG
TGGGGCAGCCGGCGAGCTTCACCGTCGATGCCTTGAACCACCGTAAATTCACCGGCCACGTTCAGCACATCTCTCCGGGC
ACCGGTTCCGAGTTCGCGTTGTTGCAGGCCGACAACGCCACCGGCAACTTCGTGAAGATCGCCCAGCGTGTGCCGGTGCG
CATTACCATCGATCCGGATCAGCAGGAGAGCGAGCGTTTGCGGCCGGGGATGTCGGTGGTGGTCAGCATTGATACGGCGG
CGGGCGACACCCAGAAACACTGA

Upstream 100 bases:

>100_bases
TGACCATGATCTGGATTTCCATCCGTGCGCTGTGGCTGATCAGCACCACCCGAGCCGTCGCCCCGACATCTTCCGTACCT
TCCAGCGGTGCCACCTCTTC

Downstream 100 bases:

>100_bases
CCTCAGGCAGCGATCATTGGCGGCAGGGTGCCTAGCACGGACACCGCCGCCACGGCGCCGATCCCCAGCAGCCATTCGAG
CATGACGCTGCGCTTGAGGG

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MTEPTTTTTNAIAATPEGVAPPSSPNTEPRSLRVRIISSLGFAGVAIVGVLIVLYAWQLPPFSSAVETTENALVRGQVTI
IGPQLSGYVFEVPVQDFQFVKEGDLLVRLDDRIYQQRLDQSLAQLAVQKASLANVVQQRNSAEATIKLRQAVVADSEAQL
RKSEADLRRNKALVNDGSVSKREMDVALAANAQSIAAVAQAKANLEIARQDLQTVIVNRGSLEAAVASAEAAVQLARIDL
SNTRIIAPRDGQLGQIGVRLGAYVNSGAQLMALVPNQKWVIANLKETQMDNVRVGQPASFTVDALNHRKFTGHVQHISPG
TGSEFALLQADNATGNFVKIAQRVPVRITIDPDQQESERLRPGMSVVVSIDTAAGDTQKH

Sequences:

>Translated_380_residues
MTEPTTTTTNAIAATPEGVAPPSSPNTEPRSLRVRIISSLGFAGVAIVGVLIVLYAWQLPPFSSAVETTENALVRGQVTI
IGPQLSGYVFEVPVQDFQFVKEGDLLVRLDDRIYQQRLDQSLAQLAVQKASLANVVQQRNSAEATIKLRQAVVADSEAQL
RKSEADLRRNKALVNDGSVSKREMDVALAANAQSIAAVAQAKANLEIARQDLQTVIVNRGSLEAAVASAEAAVQLARIDL
SNTRIIAPRDGQLGQIGVRLGAYVNSGAQLMALVPNQKWVIANLKETQMDNVRVGQPASFTVDALNHRKFTGHVQHISPG
TGSEFALLQADNATGNFVKIAQRVPVRITIDPDQQESERLRPGMSVVVSIDTAAGDTQKH
>Mature_379_residues
TEPTTTTTNAIAATPEGVAPPSSPNTEPRSLRVRIISSLGFAGVAIVGVLIVLYAWQLPPFSSAVETTENALVRGQVTII
GPQLSGYVFEVPVQDFQFVKEGDLLVRLDDRIYQQRLDQSLAQLAVQKASLANVVQQRNSAEATIKLRQAVVADSEAQLR
KSEADLRRNKALVNDGSVSKREMDVALAANAQSIAAVAQAKANLEIARQDLQTVIVNRGSLEAAVASAEAAVQLARIDLS
NTRIIAPRDGQLGQIGVRLGAYVNSGAQLMALVPNQKWVIANLKETQMDNVRVGQPASFTVDALNHRKFTGHVQHISPGT
GSEFALLQADNATGNFVKIAQRVPVRITIDPDQQESERLRPGMSVVVSIDTAAGDTQKH

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=338, Percent_Identity=36.094674556213, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1788711, Length=338, Percent_Identity=36.094674556213, Blast_Score=161, Evalue=5e-41,
Organism=Escherichia coli, GI1790519, Length=317, Percent_Identity=29.3375394321767, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI87081951, Length=321, Percent_Identity=26.1682242990654, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1789637, Length=327, Percent_Identity=28.4403669724771, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1790012, Length=294, Percent_Identity=25.8503401360544, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790024, Length=293, Percent_Identity=26.2798634812287, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40789; Mature: 40658

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPTTTTTNAIAATPEGVAPPSSPNTEPRSLRVRIISSLGFAGVAIVGVLIVLYAWQLP
CCCCCCCCCCEEEECCCCCCCCCCCCCCCHHEEEEEEHHCCHHHHHHHHHHHHHHHHCCC
PFSSAVETTENALVRGQVTIIGPQLSGYVFEVPVQDFQFVKEGDLLVRLDDRIYQQRLDQ
CCCHHHHHHHHHEEEEEEEEECCCCCCEEEEECCHHHHHHCCCCEEEEEHHHHHHHHHHH
SLAQLAVQKASLANVVQQRNSAEATIKLRQAVVADSEAQLRKSEADLRRNKALVNDGSVS
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEECCCCCC
KREMDVALAANAQSIAAVAQAKANLEIARQDLQTVIVNRGSLEAAVASAEAAVQLARIDL
HHHEEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEEEEEEC
SNTRIIAPRDGQLGQIGVRLGAYVNSGAQLMALVPNQKWVIANLKETQMDNVRVGQPASF
CCCEEEECCCCCCCEEHEEEEHEECCCCEEEEECCCCCEEEEECCCCCCCCEECCCCCCE
TVDALNHRKFTGHVQHISPGTGSEFALLQADNATGNFVKIAQRVPVRITIDPDQQESERL
EEECCCCCEEECEEEEECCCCCCCEEEEEECCCCCCEEEHCCCCCEEEEECCCCCHHHHC
RPGMSVVVSIDTAAGDTQKH
CCCCEEEEEEECCCCCCCCC
>Mature Secondary Structure 
TEPTTTTTNAIAATPEGVAPPSSPNTEPRSLRVRIISSLGFAGVAIVGVLIVLYAWQLP
CCCCCCCCCEEEECCCCCCCCCCCCCCCHHEEEEEEHHCCHHHHHHHHHHHHHHHHCCC
PFSSAVETTENALVRGQVTIIGPQLSGYVFEVPVQDFQFVKEGDLLVRLDDRIYQQRLDQ
CCCHHHHHHHHHEEEEEEEEECCCCCCEEEEECCHHHHHHCCCCEEEEEHHHHHHHHHHH
SLAQLAVQKASLANVVQQRNSAEATIKLRQAVVADSEAQLRKSEADLRRNKALVNDGSVS
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEECCCCCC
KREMDVALAANAQSIAAVAQAKANLEIARQDLQTVIVNRGSLEAAVASAEAAVQLARIDL
HHHEEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEEEEEEC
SNTRIIAPRDGQLGQIGVRLGAYVNSGAQLMALVPNQKWVIANLKETQMDNVRVGQPASF
CCCEEEECCCCCCCEEHEEEEHEECCCCEEEEECCCCCEEEEECCCCCCCCEECCCCCCE
TVDALNHRKFTGHVQHISPGTGSEFALLQADNATGNFVKIAQRVPVRITIDPDQQESERL
EEECCCCCEEECEEEEECCCCCCCEEEEEECCCCCCEEEHCCCCCEEEEECCCCCHHHHC
RPGMSVVVSIDTAAGDTQKH
CCCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]