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The map label for this gene is ampR [H]

Identifier: 77459649

GI number: 77459649

Start: 3908115

End: 3909005

Strand: Direct

Name: ampR [H]

Synonym: Pfl01_3427

Alternate gene names: 77459649

Gene position: 3908115-3909005 (Clockwise)

Preceding gene: 77459641

Following gene: 77459650

Centisome position: 60.7

GC content: 62.74

Gene sequence:

>891_bases
ATGTACAAACGATACCCGTCGGTGCAGTCCATGAGCGCGTTCATCCACGCGGCGCGCAGCGGCAGTTTCTCCAGCGCCGC
GCGCAAGCTCGACCTGACCCACAGCGCGATCAGCCAGCAGATCCGCGCCCTGGAAGACTTCATCGGCCAGCCGCTGTTCG
TGCGCGAAGGCGGCGGCAGCAACCTGACCGATGCCGGGCAGCTGTTTGCCAGCGTACTGTCGGATGGGCTGGCGCAGATT
GACCGGGCGTTGTCTTCGGTGAAAAACCGCAGCGTGGCCCAGCGTCTGACCCTGGACGTGGACAGCGAACTGGCGCAGAG
CTGGCTCAATCCGCGTCTGCCGCAACTGCTCGACGGCTTGCCGGATTACGAAGTGACAATCCTGTCGATGCCGCGCAGTG
ATCGCAGCACGCTCGAACGGGTGAACCTGTTGCTGCGCTATGGCTATGGCGATTGGGACGATTGCGAAATGACGCAGATC
TGTGGGGATCGGGTGTTGGCGGTGGCGTCTCCGGCGTTGCTTGAGCGGCATGGTTTGCAGGTGCCACTGAGCCCGGCGCA
GATTCTTGAACTGCCACTGTTGGGGTATACGCGCAGGTCGTGGATTCCGTGGCTGGATGCGGCGGGGATGACACCGACCG
AACCGCCGGCGCGGGTGATTTTCGATAATGCGGCGAATCTGATTGCGGCGGCTGAAGCCGGCGTCGGTGCGGGACTGGTG
CGTGGATTGCTGGCTGCCGATGCGTTGCGCAGCGGGCGGCTGGTGGCGTTGAACCAAGCGCAGATTGCGGCGCATTACAA
TCTTTATGCGGTGTGGCCGCATGGGCAGGCTGAGCGAGTGGCACCTGTTGTTGAAGTGATCCAGCAGTTGGCCATGCAAA
CACTTCACTGA

Upstream 100 bases:

>100_bases
CGGCCCGTCTTTGTGCGGGCCTGGTGACCGATTATTGGAACCCCCGGCAACGCGCGGCCAGCGACGATTTCTGCCAACCG
GTGTAAGCAGGACTAACAAC

Downstream 100 bases:

>100_bases
CGCCATCGCTGGCAAGCCCATATCGAACAAGTATCACCACCCCACCCAATTAATATTGTACGAACCCTCCCCCGCCTTCC
AATACTCGCCTCAAGCAACC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MYKRYPSVQSMSAFIHAARSGSFSSAARKLDLTHSAISQQIRALEDFIGQPLFVREGGGSNLTDAGQLFASVLSDGLAQI
DRALSSVKNRSVAQRLTLDVDSELAQSWLNPRLPQLLDGLPDYEVTILSMPRSDRSTLERVNLLLRYGYGDWDDCEMTQI
CGDRVLAVASPALLERHGLQVPLSPAQILELPLLGYTRRSWIPWLDAAGMTPTEPPARVIFDNAANLIAAAEAGVGAGLV
RGLLAADALRSGRLVALNQAQIAAHYNLYAVWPHGQAERVAPVVEVIQQLAMQTLH

Sequences:

>Translated_296_residues
MYKRYPSVQSMSAFIHAARSGSFSSAARKLDLTHSAISQQIRALEDFIGQPLFVREGGGSNLTDAGQLFASVLSDGLAQI
DRALSSVKNRSVAQRLTLDVDSELAQSWLNPRLPQLLDGLPDYEVTILSMPRSDRSTLERVNLLLRYGYGDWDDCEMTQI
CGDRVLAVASPALLERHGLQVPLSPAQILELPLLGYTRRSWIPWLDAAGMTPTEPPARVIFDNAANLIAAAEAGVGAGLV
RGLLAADALRSGRLVALNQAQIAAHYNLYAVWPHGQAERVAPVVEVIQQLAMQTLH
>Mature_296_residues
MYKRYPSVQSMSAFIHAARSGSFSSAARKLDLTHSAISQQIRALEDFIGQPLFVREGGGSNLTDAGQLFASVLSDGLAQI
DRALSSVKNRSVAQRLTLDVDSELAQSWLNPRLPQLLDGLPDYEVTILSMPRSDRSTLERVNLLLRYGYGDWDDCEMTQI
CGDRVLAVASPALLERHGLQVPLSPAQILELPLLGYTRRSWIPWLDAAGMTPTEPPARVIFDNAANLIAAAEAGVGAGLV
RGLLAADALRSGRLVALNQAQIAAHYNLYAVWPHGQAERVAPVVEVIQQLAMQTLH

Specific function: Regulates the expression of the beta-lactamase gene. Represses cephalosporinase (AmpC) in the presence of beta-lactams and induces it in the absence of them [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=279, Percent_Identity=29.7491039426523, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788706, Length=287, Percent_Identity=27.5261324041812, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1786448, Length=268, Percent_Identity=28.7313432835821, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI145693193, Length=282, Percent_Identity=25.177304964539, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32203; Mature: 32203

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKRYPSVQSMSAFIHAARSGSFSSAARKLDLTHSAISQQIRALEDFIGQPLFVREGGGS
CCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
NLTDAGQLFASVLSDGLAQIDRALSSVKNRSVAQRLTLDVDSELAQSWLNPRLPQLLDGL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHCCC
PDYEVTILSMPRSDRSTLERVNLLLRYGYGDWDDCEMTQICGDRVLAVASPALLERHGLQ
CCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHCCCC
VPLSPAQILELPLLGYTRRSWIPWLDAAGMTPTEPPARVIFDNAANLIAAAEAGVGAGLV
CCCCHHHHHHCCCCCCCHHCCCCCCCCCCCCCCCCCHHEEECCHHHHHHHHHCCCHHHHH
RGLLAADALRSGRLVALNQAQIAAHYNLYAVWPHGQAERVAPVVEVIQQLAMQTLH
HHHHHHHHHCCCCEEEECCHHEEEECEEEEECCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYKRYPSVQSMSAFIHAARSGSFSSAARKLDLTHSAISQQIRALEDFIGQPLFVREGGGS
CCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
NLTDAGQLFASVLSDGLAQIDRALSSVKNRSVAQRLTLDVDSELAQSWLNPRLPQLLDGL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHCCC
PDYEVTILSMPRSDRSTLERVNLLLRYGYGDWDDCEMTQICGDRVLAVASPALLERHGLQ
CCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHCCCC
VPLSPAQILELPLLGYTRRSWIPWLDAAGMTPTEPPARVIFDNAANLIAAAEAGVGAGLV
CCCCHHHHHHCCCCCCCHHCCCCCCCCCCCCCCCCCHHEEECCHHHHHHHHHCCCHHHHH
RGLLAADALRSGRLVALNQAQIAAHYNLYAVWPHGQAERVAPVVEVIQQLAMQTLH
HHHHHHHHHCCCCEEEECCHHEEEECEEEEECCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2786868; 1943705 [H]